Simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcusspecies

ABSTRACT

The antifungal activity of some statins against different fungal species has been reported. Thus, at the first moment, the in vitro antifungal activity of simvastatin, atorvastatin and pravastatin was tested againstCandida spp. and Cryptococcus spp. Then, in a second approach, considering that the best results were obtained for simvastatin, this drug was evaluated in combination with antifungal drugs against planktonic growth and tested against biofilms ofCandida spp. and Cryptococcus spp. Drug susceptibility testing was performed using the microdilution broth method, as described by the Clinical and Laboratory Standards Institute. The interaction between simvastatin and antifungals against planktonic cells was analyzed by calculating the fractional inhibitory concentration index. Regarding biofilm susceptibility, simvastatin was tested against growing biofilm and mature biofilm of one strain of each tested yeast species. Simvastatin showed inhibitory effect against Candida spp. andCryptococcus spp. with minimum inhibitory concentration values ranging from 15.6 to 1000 mg L^-1 and from 62.5 to 1000 mg L^-1, respectively. The combination of simvastatin with itraconazole and fluconazole showed synergism against Candidaspp. and Cryptococcus spp., while the combination of simvastatin with amphotericin B was synergistic only againstCryptococcus spp. Concerning the biofilm assays, simvastatin was able to inhibit both growing biofilm and mature biofilm ofCandida spp. and Cryptococcus spp. The present study showed that simvastatin inhibits planktonic cells and biofilms ofCandida and Cryptococcus species.

Keywords:
Candida; Cryptococcus; Simvastatin; Antifungal activity; Biofilm

Introduction

The incidence of invasive fungal infections, especially those caused by opportunistic fungi of the genus Candida and Cryptococcus, has proportionally increased with the increase in the number of hosts with impaired immunity. ^{1 2 3 and 4} In addition, in vitroresistance to antifungal drugs among Candida spp. andCryptococcus spp. strains recovered from humans and animals has been reported. ^{5 6 7 8 9 10 and 11}

This scenario motivates the search for new compounds with antifungal potential. Originally, the first statins were described as metabolites of microorganisms with the ability to lower blood cholesterol.¹²Later, it was demonstrated that these compounds reduce the growth of several fungal species,^{13 14 and 15} including the yeastsCandida spp. and Cryptococcus neoformans ¹⁶ and the filamentous fungiMucor spp. and Rhizopus spp. ¹⁷ In addition, it has also been reported that the administration of statins to hospitalized patients increases survival ¹⁸ and decreases Candidaburden in diabetic patients. ¹⁹

Although the antifungal potential of statins has already been addressed in previous reports, studies involving the effect of statins on fungal biofilms are needed to obtain a better knowledge on the antifungal potential of these compounds. Hence, this study evaluated the effect of the statins simvastatin, atorvastatin, and pravastatin on planktonic cells of Candida spp. andCryptococcus spp. Considering that the best results were obtained for simvastatin, this drug was evaluated in combination with antifungal drugs against planktonic growth. In addition, simvastatin was tested against biofilms of Candida spp. and Cryptococcus spp.

Materials and methods

Microorganisms

For this study, 51 strains of Candida spp. (16 Candida albicans; 12 Candida tropicalis; 11Candida krusei; 12 Candida parapsilosis sensu lato), and 25 strains of Cryptococcus spp. (13C. neoformans - serotypes A, D and AD; and 12Cryptococcus gattii - serotypes B and C) isolated from animals were used. The isolates belong to the culture collection of the Specialized Medical Mycology Center, Brazil. The purity and identity of theCandida spp. strains were confirmed by growth on chromogenic medium and microscopical and biochemical features. ²⁰ For the Cryptococcusspp. strains, capsule formation, melanin production, and biochemical testing were evaluated and the serotype of each strain was assessed by PCR. ²¹

Susceptibility testing of planktonic cells

Susceptibility assays were performed using the broth microdilution method, as described by the document M27-A3 of the Clinical and Laboratory Standards Institute.²² The tests were performed in duplicate and visually read after 48 h of incubation at 35 °C. The strainsC. parapsilosis ATCC 22019 and C. kruseiATCC 6258 were included as quality control for all tests. ²²

Inocula were prepared to obtain a final concentration of 0.5-2.5 × 10³cells mL^-1.²² The statins simvastatin (Medley Indústria Farmacêutica Ltda, Campinas, SP, Brazil), atorvastatin (Laboratórios Pfizer Ltda, São Paulo, SP, Brazil), and pravastatin (Bristol-Myers-Squibb, Nova York, NY, USA) and the antifungal drugs amphotericin B (Sigma Chemical Corporation, St Louis, USA), itraconazole (Janssen Pharmaceutica, Beerse, Belgium), and fluconazole (Pfizer Pharmaceuticals, New York, USA) were tested against all strains.

To obtain the stock-solutions of each drug, atorvastatin, pravastatin, and fluconazole were diluted with sterile distilled water, and amphotericin B and itraconazole were diluted with dimethylsulfoxide (DMSO). Simvastatin was activated from its lactone prodrug form through hydrolysis in ethanolic NaOH (15% (v/v) ethanol, 0.25% (w/v) NaOH), at 60 °C, for 1 h.¹⁵ The concentration range tested was 3.9-2000 µg mL^-1 for simvastatin, 19.5-10,000 µg mL^-1 for atorvastatin, 97.6-50,000 µg mL^-1 for pravastatin, 0.0312-64 µg mL^-1 for amphotericin B and itraconazole, and 0.25-256 µg mL^-1 for fluconazole. The minimum inhibitory concentration (MIC) was defined as the lowest drug concentration able to inhibit 100% of fungal growth for amphotericin B and 50% inhibition of fungal growth when compared to the free-drug azoles²² and statins control.

As simvastatin provided the best antifungal results, we evaluated the interaction between this drug and the antifungal drugs against the tested yeasts. For drug interaction studies, simvastatin was tested with each azole by broth microdilution method, using the MIC of each tested drug alone as the highest concentrations tested in combination. The concentrations of the drugs in combination ranged from 0.03 to 1000, 0.00024 to 2, 0.00006 to 64 and 0.00048 to 256 µg mL^-1 for simvastatin, amphotericin B, itraconazole and fluconazole, respectively. The reading criteria were the same as for the antifungal drugs alone, namely 100% inhibition when combined with amphotericin B and 50% inhibition when combined with azoles. The interaction between the drugs was analyzed by calculating the fractional inhibitory concentration index (FICI), with values ≤0.5 indicating synergism.²³

Susceptibility test of sessile cells

Simvastatin was tested against growing biofilms and mature biofilms ofCandida spp. and Cryptococcus spp. Amphotericin B and itraconazole were used in all tests as control drugs for biofilm inhibition. The tests were performed in triplicate using one biofilm-producing strain of each tested fungal species (C. albicans, C. tropicalis, C. parapsilosis, C. krusei, C. neoformans andC. gattii), according to the methodology described by Chatzimoschou et al., ²⁴ with some modifications. Briefly, strains were grown on Sabouraud dextrose agar for 48 h at 30 °C and then subcultured into Sabouraud dextrose broth for 24 h, at 30 °C, under agitation at 150 rpm. After this period, the suspensions were centrifuged at 3000 rpm for 10 min, the supernatant was discarded, and the pellet was washed twice with sterile PBS. Then, the pellet was resuspended in RPMI 1640 medium (Gibco-BRL, USA), reaching a concentration of 1 × 10⁶ cells mL^-1. Tests were performed in 96-well polystyrene plates.

To evaluate the effect of simvastatin, amphotericin B, and itraconazole on growing biofilm, 100 µL of the fungal suspension was exposed to 100 µL of simvastatin and incubated at 35 °C for 48 h. The tested concentrations were based on the MIC obtained for each drug against fungal planktonic growth, including MIC, 10xMIC and 50xMIC. On the other hand, to evaluate the effect of simvastatin and the antifungals alone against mature biofilm, 100 µL of the fungal suspension was added to 100 µL of RPMI 1640 medium and incubated at 35 °C for 48 h. Then, the mature biofilms were exposed to simvastatin, amphotericin B, and itraconazole and incubated at 35 °C for 48 h. The tested concentrations against mature biofilms were 10xMIC, 50xMIC and 100xMIC. For all tests, drug-free growth control for each strain was included.

After 48 h of drug exposure, the growing and mature biofilms were submitted to the following procedures: supernatants were collected and reserved for further analysis, and plates were washed twice with sterile PBS Tween 20 (0.05%, v/v) solution to remove non-adhered cells. Then, the biofilm viability was evaluated through XTT assay, according to Martinez and Casadevall,²⁵ with modifications. Stock solutions of XTT (1 mg mL^-1) were previously prepared, filtrated and stocked at -20 °C, until used. Menadione (Sigma) (0.4 mM in acetone) was prepared at the moment of use. Afterwards, 50 µL of sterile PBS, 75 µL of XTT solution, and 6 µL of menadione solution were added to each well. Plates were incubated at 35 °C during 5 h, in the dark, and then XTT was all transferred to a new plate and read in a spectrophotometer at 492 nm.

Statistical analysis

For analysis of the MIC data of drugs against planktonic cells, Student'st test for independent and paired samples was used. To check the variation of the MIC values of the drugs in combination, as well as the FICI value, Student's t test for paired samples was also used. Regarding the biofilm assay, all tests were made in triplicate and results were evaluated by ANOVA and Tukey's multiple comparison post-test.p-Values < 0.05 were considered statistically significant.

Results

Susceptibility test of planktonic cells

Among the tested statins, simvastatin showed the lowest MIC, with geometric means varying from 29.45 to 567.16 and from 62.5 to 500 mg L^-1 against the genera Candida and Cryptococcus, respectively ( Table 1). Atorvastatin showed better results against Candida species, when compared toCryptococcus spp., with MIC geometric means varying from 52.06 to 1682.37 mg L^-1 against Candida spp. and from 3886.02 to >10,000 µg mL^-1 againstCryptococcus spp. ( Table 1). As for pravastatin, the MIC geometric means varied from 2159.24 to >50,000 mg L^-1 against Candida spp. and from 44079.56 to >50,000 mg L^-1 against Cryptococcus (Table 1). MIC geometric means for the classic antifungals against Candida spp. varied from 0.343 to 1.624 mg L^-1 for amphotericin B, from 0.046 to 15.021 mg L^-1 for itraconazole, and from 0.659 to 82.346 mg L^-1for fluconazole. MIC geometric means for classic antifungals againstCryptococcus spp. varied from 0.125 to 0.735 mg L^-1 for amphotericin B, from 0.189 to 1 mg L^-1 for itraconazole, and from 6.498 to 64 mg L^-1 for fluconazole ( Table 1).

Table 1 -
Geometric means of the minimum inhibitory concentrations (MIC) of statins and antifungal agents against Candida spp. and Cryptococcus spp.

Strains (n)	MIC geometric mean (µg mL-1)
	Statins			Antifungals
	Simvastatin	Atorvastatin	Pravastatin	Amphotericin B	Itraconazole	Fluconazole
Candida species
C. albicans (16)	29.45	52.06	2159.24	0.561	5.992	21.357
C. tropicalis (12)	70.12	165.34	21022.41	0.343	15.021	82.346
C. krusei (11)	567.16	755.06	>50,000	1.624	0.072	12.126
C. parapsilosis sensu lato (12)	235.97	1491.37	>50,000	0.707	0.059	0.891
Cryptococcus species
C. neoformans A a (11)	500	3886.02	44079.56	0.536	0.189	6.498
C. neoformans D (1)	250	>10,000	>50,000	0.25	0.25	8
C. neoformans AD (1)	62.5	>10,000	>50,000	0.125	0.5	8
C. gatti B (11)	500	5325.21	44079.56	0.735	0.315	34.562
C. gatti C (1)	500	>10,000	>50,000	1	1	64

Results for the in vitro interaction between simvastatin and antifungal drugs against these yeasts are shown in Table 2. In general, a synergistic interaction was observed between simvastatin and both azoles against C. albicans(n = 10/10), C. tropicalis(n = 11/11) and C. parapsilosis sensu lato (n = 12/12) (p < 0.05). Concerning C. krusei, only the combination of simvastatin and itraconazole was synergistic (n = 9/10) (p < 0.05). As for Cryptococcus spp., synergistic interactions were observed between simvastatin and the three antifungals tested against C. gattii (simvastatin/amphotericin B and simvastatin/fluconazole: n = 7/7; simvastatin/itraconazole: n = 5/7) and C. neoformans (simvastatin/amphotericin B: n = 11/11; simvastatin/itraconazole: n = 9/11; simvastatin/fluconazole:n = 10/11) (p < 0.05).

Table 2 -
Geometric means of the minimum inhibitory concentrations (MIC) of the combination of simvastatin and the antifungal amphotericin B, itraconazole, or fluconazole against Candida spp. and Cryptococcus spp.

Species (n)	Drugs	MIC (isolated) (µg mL-1)		MIC (combination) (µg mL-1)		FICI	Number of strains showing synergism
		SIM	Antifungal	SIM	Antifungal
Candida albicans (10)
	SIM/AMB	29.11	0.616	24.06	0.595	1.741	0/10
	SIM/ITC	29.11	9.189	1.042	0.329	0.071	10/10
	SIM/FLC	29.11	21.112	1.695	0.116	0.116	10/10
Candida tropicalis (11)
	SIM/AMB	75.47	0.3426	55.637	0.354	1.37	1/11
	SIM/ITC	75.47	15.021	1.327	0.266	0.035	11/11
	SIM/FLC	75.47	82.347	4.425	4.832	0.117	11/11
Candida krusei (10)
	SIM/AMB	574.35	1.624	435.28	1.231	1.515	0/10
	SIM/ITC	574.35	0.072	116.61	0.014	0.416	9/10
	SIM/FLC	574.35	12.126	233.26	4.925	0.812	3/10
Candida parapsilosis sensu lato(12)
	SIM/AMB	235.97	0.707	78.74	0.236	0.667	7/12
	SIM/ITC	235.97	0.059	41.68	0.010	0.353	12/12
	SIM/FLC	235.97	0.891	49.58	0.187	0.420	12/12
Cryptococcus neoformans(11)
Serotypes: A(9); D(1); AD(1)	SIM/AMB	388.6	0.4139	35.42	0.0377	0.182	11/11
	SIM/ITC	388.6	0.1943	70.86	0.0354	0.365	9/11
	SIM/FLC	388.6	6.622	21.38	0.3648	0.11	10/11
Cryptococcus gattii (7)
Serotypes: B(6); C(1)	SIM/AMB	500	0.8203	19.025	0.0312	0.076	7/7
	SIM/ITC	500	0.4102	84.0803	0.0689	0.336	5/7
	SIM/FLC	500	39.008	38.051	2.972	0.152	7/7

Susceptibility test of sessile cells

Regarding the action of simvastatin against biofilm of Candidaspp., simvastatin inhibited growing biofilms at concentrations greater than 10xMIC ( Fig. 1). Amphotericin B caused significant decrease in metabolic activity of growing biofilms at 10xMIC and 50xMIC, while itraconazole caused inhibition at all tested concentrations (p < 0.05). As for mature biofilms, simvastatin caused significant decrease in metabolic activity ( Fig. 1) (p < 0.05), at concentrations above 50xMIC. Amphotericin B inhibited mature biofilms at all tested concentrations, while itraconazole only at 50xMIC and 100xMIC (p < 0.05).

Fig. 1 -.
In vitro effect of simvastatin (SIM), amphotericin B (AMB), and itraconazole (ITC) at 3 different concentrations on biofilm formation (A) and mature biofilm (B) ofCandida spp. Black bars: positive control; MIC: minimum inhibitory concentration. Absorbance of XTT (492 nm). *Represents statistically significant difference (p< 0.05) when compared to the positive control. Data expressed as mean ± SEM.

Regarding the genus Cryptococcus, simvastatin inhibited growing biofilms at all tested concentrations ( Fig. 2) (p < 0.05), similar to what was observed when amphotericin B and itraconazole were used (p < 0.05). Concerning mature biofilms, simvastatin caused significant decrease in metabolic activity of Cryptococcus biofilm at 50xMIC and 100xMIC ( Fig. 2) (p < 0.05). Amphotericin B inhibited mature biofilms at all tested concentrations (p < 0.05), while itraconazole did not decrease biofilm metabolic activity at any tested concentration.

Fig. 2 -.
In vitro effect of simvastatin (SIM), amphotericin B (AMB), and itraconazole (ITC) at 3 different concentrations on biofilm formation (A) and mature biofilm (B) ofCryptococcus spp. strains. Black bars: positive control; MIC, minimum inhibitory concentration. Absorbance of XTT (492 nm). *Represents statistical significant difference (p < 0.05) when compared to the positive control. Data expressed as mean ± SEM.

Discussion

This study shows the inhibitory activity of simvastatin on the growth of yeasts of the genera Candida and Cryptococcus with an inhibitory effect against both planktonic cells and biofilms. The MICs of simvastatin against C. albicans and C. tropicaliswere similar to the serum levels of the drug, when administered to control blood cholesterol. ²⁶ C. albicans and C. tropicalis are important fungal pathogens commonly isolated from candidemia. ^{27 and 28} Simvastatin and atorvastatin have been described inhibiting Candida spp. and the filamentous fungusAspergillus fumigatus. ¹³ This work confirms the action of these two drugs, especially simvastatin against Candida spp. and Cryptococcusspp. However, the growth of these fungi was not inhibited by pravastatin. The use of statins deregulates cellular production of isoprenoid, ¹³ which leads to mitochondrial dysfunction, respiratory deficit, ²⁹ and changes in lipid structure and in the dynamics of plasma membrane of C. albicans cells. ³⁰

There is no synergistic interaction when simvastatin is associated with amphotericin B against most Candida spp. strains. Statins reduce the amount of fungal ergosterol, which may lead to decreased activity of amphotericin B, since ergosterol is the target molecule for this antifungal drug and a decrease in the amount of this molecule is one of the mechanisms developed by amphotericin B resistant Candida spp. However, synergism between simvastatin and amphotericin B was observed against strains of Cryptococcus spp., in line with previous reports with the filamentous fungi Rhizopus oryzae and Aspergillus flavus. ³¹ These contradictory findings still need to be elucidated.

In general, when simvastatin is associated with azoles (i.e. itraconazole or fluconazole) there is synergism against strains of Candida spp. andCryptococcus spp. However, probably due to the intrinsic resistance of C. krusei to fluconazole, synergism between simvastatin and fluconazole was not observed against thisCandida species. The interaction between statins and azoles has been reported against the yeasts Saccharomyces cerevisiae ³² and Candida spp., ³³ and the filamentous fungiAspergillus spp., Mucor spp. andRhizopus spp. ^{17 and 33} Synergism between these two pharmacological groups is most likely associated with the combined action of the drugs in reducing fungal ergosterol, by acting at different moments in the pathway of ergosterol biosynthesis. ¹¹ In addition, the reduction of endogenous sterol due to the action of statins increases cell membrane permeability in order to increase absorption of exogenous sterol, as a compensatory mechanism, and, simultaneously, the entrance of azoles in the cell is facilitated. ³⁴

Biofilm production is considered an important virulence factor ofCandida spp. ³⁵ and it contributes for the persistence of infections. ³⁶ It has been demonstrated that simvastatin also inhibits growing and mature biofilms of Candida spp. and Cryptococcusspp. strains when used alone. Liu et al. ³⁷showed that simvastatin inhibited biofilm production of C. albicansafter 16-h-incubation, suggesting that at least one mechanism of inhibition involves interference with ergosterol biosynthesis. On the other hand, there are no reports of the effect of simvastatin on biofilms of Cryptococcus spp. Additional studies are needed to better understand the action of simvastatin against yeast biofilms.

Studies have shown that amphotericin B inhibits fungal biofilms^{25 and 38} causing apoptosis of the cells inCandida biofilms. ³⁹ In our study, growing and mature biofilms of Candida spp. andCryptococcus spp. were inhibited by amphotericin B. Although many authors have reported that azoles do not inhibit fungal biofilms, ^{25 and 38} the present study demonstrated the inhibition of growing and mature biofilms of Candida spp. by itraconazole. As for Cryptococcus spp., itraconazole only inhibited growing biofilms.

Although several reports have demonstrated in vitro activity of statins agents against many clinical relevant yeast and mold species, as well as the synergistic effect of statins with different antifungal drugs, clinical studies are scarce. ⁴⁰ Spanakis et al. ¹⁹ showed that use of statins decreased the incidence of cultures positive for Candida species among patients with type 2 diabetes mellitus who underwent gastrointestinal surgery. In contrast, no beneficial effects were observed for statins in a study of patients with candidemia. ⁴¹ However, these studies were performed with different patient groups and were inconclusive. Thus, further studies aiming to evaluate the benefits of statin in antifungal therapy are required.

The present study showed the activity of statins against Candida andCryptococcus species, with particular emphasis on simvastatin, isolated and combined with classical antifungals. In addition, it was also demonstrated that simvastatin was able to inhibit growing and mature biofilms ofCandida spp. and Cryptococcus spp.

Acknowledgements

This work was supported by grants from the National Council for Scientific and Technological Development (CNPq; Brazil; Processes 307606/2013-9; 443167/2014-1; 445670/2014-2) and CAPES (AE1 - 0052-000650100/11).

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Publication Dates

• Publication in this collection
  Sep-Oct 2015

History

• Received
  02 Apr 2015
• Accepted
  01 June 2015