Exogenous application of phytohormones mitigates the effect of salt stress on Carica papaya plants

Aplicação exógena de fitormônios mitiga o efeito do estresse salino em plantas de Carica papaya

ABSTRACT

The salinity has complex effects on the physiological, nutritional and hormonal interactions of plants. This study aimed to evaluate the mitigating action of exogenous application of different types and concentrations of phytohormones on Carica papaya under salt stress. The experiment was conducted in greenhouse, using a randomized block design with treatments formed from a 2 x 3 x 5 factorial scheme, relative to two concentrations of salts in the irrigation water (0.6 and 2.4 dS m^-1), three types of phytohormones [Auxin = indole-3-acetic acid; Cytokinin = CPPU (N-(2-chloro-pyridyl)-N-phenylurea) and Gibberellin = gibberellic acid(AG₃)], and five concentrations of the phytohormone (0, 12.5, 25.0, 37.5 and 50.0 µM L^-1), with three repetitions, totaling 90 plots, and the experimental unit consisted of six plants. C. papaya cv. ‘Sunrise Solo’ was grown on polyethylene trays of 162 cells, with capacity for 50 mL. The plants were evaluated for survival percentage and phytomass accumulation until 25 days after sowing. Increase in irrigation water salinity has deleterious effects on C. papaya plants regardless of the application of phytohormones. Exogenous application of gibberellin has a positive effect in mitigating salt stress on C. papaya plants, greater than the effects of auxins and cytokinins. Exogenous application of gibberellin and auxin at concentration of 37.5 µM L^-1 mitigates the effects of salinity on C. papaya plants. Exogenous application of cytokinin at concentrations of 12.5 to 50.0 µM L^-1 is not viable for C. papaya plants.

Key words:
auxins; cytokinins; gibberellins; salinity

RESUMO

A salinidade tem efeitos complexos nas interações fisiológicas, nutricionais e hormonais das plantas. Objetivou-se avaliar a ação mitigadora da aplicação exógena de diferentes tipos e concentrações de hormônios vegetais em plantas de Carica papaya sob estresse salino. O experimento foi realizado em casa de vegetação por meio do delineamento experimental de blocos casualizados com tratamentos formados a partir de esquema fatorial 2 x 3 x 5, relativo a duas concentrações de sais na água de irrigação (0,6 e 2,4 dS m^-1), três tipos de hormônios vegetais [Auxina = ácido indol-3-acético; Citocinina = CPPU (N-(2-cloro-piridil)-N-feniluréia) e Giberelina = ácido giberélico(AG₃)], e cinco concentrações do hormônio vegetal (0; 12,5; 25,0; 37,5 50,0 µM L^-1), com três repetições, totalizando 90 parcelas; sendo a unidade experimental composta por seis plantas. O cultivo do C. papaya, cv. Sunrise Solo, foi realizado em bandejas de polietileno de 162 cédulas, com capacidade de 50 mL. As plântulas foram avaliadas quanto a percentagem de sobrevivência e o acúmulo de fitomassa até aos 25 dias após semeadura. O aumento da salinidade da água de irrigação exerce efeitos danosos nas plantas de C. papaya independente da aplicação de hormônios vegetais. A aplicação exógena da giberelina tem efeito positivo na mitigação do estresse salino em plântulas de C. papaya, superiores aos efeitos das auxinas e citocininas. As aplicações exógenas de giberelina e auxina na concentração de 37,5 µM L^-1 mitigam os efeitos da salinidade sobre as plântulas de C. papaya. A aplicação exógena da citocinina nas concentrações de 12,5 a 50,0 µM L^-1 é inviável para as plântulas de C. papaya.

Palavras-chave:
auxinas; citocininas; giberelinas; salinidade

Introduction

Carica papaya L. is a plant typical of tropical climate, belonging to the Caricaceae family, with great socioeconomic and food importance in Brazil and worldwide. Currently, Brazil is the second largest papaya producer in the world, being responsible for 12.5% of the global production, with annual production estimated at 1,582,638 tons (FAO, 2018). C. papaya plants are moderately sensitive to salt stress since Sá et al. (2013a,b), evaluating the initial growth of two C. papaya cultivars irrigated with saline water, state that waters with salinity above 2.0 dS m^-1 are not suitable for irrigation of C. papaya in the seedling stage, drastically liming its growth.

The osmotic and ionic effects imposed by salt stress directly affect plant development, both by the limitation in water absorption and the toxicity by specific ions, especially sodium and chloride. Thus, excessive accumulation of salts has complex effects on the physiological, nutritional and hormonal interactions of plants (Muns & Tester, 2008; Syvertsen & Garcia-Sanchez, 2014; Taiz et al., 2015). Hormonal interactions are complex and several researchers currently use plant-growth regulators (phytohormones) to reduce or eliminate the negative effects of salinity and therefore try to maintain the rates of cell expansion and division, as well as the translocation of compatible solutes (Tabur & Demir, 2010; Javid et al., 2011). Auxins and cytokinins are phytohormones indispensable for vitality, growth and development of plants, and any alteration affecting their synthesis and homeostasis leads to death of the plant tissue. These phytohormones have recently been shown to be able to control plant adaptation to salt stress (Ha et al., 2012; Taiz et al., 2015). Javid et al. (2011) observed that exogenous application of indole-3-acetic acid (auxin) and kinetin (cytokinin) increase the production and concentration of starch in rice cultivars subjected to salt stress. Gibberellin (GA) is a plant regulator with regulatory functions in growth, influencing the growth rates of leaves, internodes, hypocotyls and roots (Nelissen et al., 2012; Taiz et al., 2015; Ubeda-Tomás et al., 2009). Xu et al. (2016) found that exogenous application of gibberellic acid mitigates salt stress in the initial growth of Festuca arundinacea genotypes. Given the above, this study aimed to evaluate the mitigating action of exogenous application of different types and concentrations of phytohormones in C. papaya plants under salt stress.

Material and Methods

The experiment was conducted in a greenhouse at the Centro de Ciências e Tecnologia Agro-alimentar da Universidade Federal de Campina Grande (CCTA-UFCG), Campus of Pombal, PB, located in the semi-arid region of Brazil (6º 48’ 16’’ S, 37º 49’ 15” W, at altitude of 175 m).

The experimental design was randomized blocks, with treatments formed from a 2 x 3 x 5 factorial scheme, relative to two concentrations of salts in the irrigation water based on water electrical conductivity (ECw) (S₁ = 0.6 and S₂ = 2.4 dS m-1) (Sá et al., 2013b), three types of phytohormones [Auxin = indole-3-acetic acid; Cytokinin = CPPU (N-(2-chloro-pyridyl)-N-phenylurea) and Gibberellin = gibberellic acid(AG₃)], and five concentrations of phytohormone (0, 12.5, 25.0, 37.5 and 50.0 µM L^-1), which were repeated in three blocks, in a total of 90 plots, each one with six plants.

Plants were grown on polyethylene trays of 162 cells, with capacity for 50 mL, washed with sodium hypochlorite at 10%, containing substrate composed of a mixture of soil (Entisol fluvent) and commercial substrate (Top Plant®), at 1:1 proportion, respectively, whose chemical characteristics were determined based on Donagema et al. (2011) and are described in Table 1.

Table 1
Chemical characteristics of the substrate used in the production of Carica papaya seedlings

K+	Na+	Ca+2	Mg+2	Al+3	H+ + Al+3	SB	CEC	pH (H2O)	ECse (dS m-1)	P (mg dm-3)	ESP (%)	OM (g kg-1)
(cmolc dm-3)
0.45	0.34	9.20	7.50	0	1.16	17.49	17.49	6.42	0.82	19	1.82	52

• P, K⁺, Na⁺ - Extracted with Mehlich-1; Al⁺³, Ca⁺², Mg⁺² - Extracted with 1.0 mol L^-1 KCl; SB = Ca⁺² + Mg⁺² + K⁺ + Na⁺; H⁺ + Al⁺³ - Extracted with 0.5 mol L^-1 calcium acetate at pH 7.0; CEC = SB + H⁺ +Al⁺³; OM - Walkley-Black wet digestion; ECse - Electrical conductivity of the saturation extract; ESP - Exchangeable sodium percentage

The cultivar of C. papaya used in the experiment was ‘Sunrise Solo’, belonging to the group ‘Solo’, described as the most tolerant in a study conducted by Sá et al. (2013b), evaluating the tolerance to salt stress of C. papaya cultivars from the groups ‘Solo’ and ‘Formosa’ in the stage of seedling production. Sowing was carried out using three seeds per cell, and thinning was performed when plants had one true leaf, leaving only one plant in each pot. The period for seedling emergence stabilization was 15 days.

At 15 days after sowing, irrigations with salinized waters began and were performed twice a day, so as to maintain the soil with moisture content close to the maximum retention capacity, based on drainage lysimetry, and to the water depth calculated was added a leaching fraction (LF) of 0.20 (Sá et al., 2016). The volume applied (Va) per pot was obtained by the difference between the previous irrigation depth applied (La) and mean drainage (d), divided by the number of pots (n), as shown in Eq. 1:

$$Va=\frac{La-\left(\frac{d}{n}\right)}{\left(1-LF\right)}$$ (1)

Saline waters were prepared by the addition of salts to the local supply water, ECw = 0.27 dS m^-1. The salts used in the water salinization process were NaCl, CaCl₂.2(H₂O) and MgCl₂.6(H₂O), at proportion of 7 x 2 x 1, respectively, as recommended by Medeiros (1992), because it represents the average composition of the waters in Northeast Brazil. The salts were added until obtaining the desired level of salinity, which was calibrated with a benchtop conductivity meter, and the electrical conductivity was adjusted at 25 ºC.

Exogenous application of phytohormones was carried out as the irrigation with saline water began. The different concentrations of each phytohormone were prepared (diluted) in deionized water at the Laboratory of Plant Physiology of the Universidade Federal de Campina Grande. Phytohormones were applied using manual sprayers on plant shoots, 15 mL per plot.

At 5 and 10 days of treatment application, corresponding to 20 and 25 days after sowing, the percentage of survival of C. papaya plants was determined based on the ratio between the number of living plants in the plot and the number of dead plants, with values expressed in percentage. At 10 days after applying the treatments, total dry matter accumulation (TDM) was determined. Plants were collected, placed in paper bags and dried in a forced-air oven at temperature of 65 ºC until constant weight. Then, the material was weighed on an analytical scale with 0.0001 g precision at the Laboratory of Plant Physiology of the Universidade Federal de Campina Grande.

At the end of the experiment, electrical conductivity and pH were measured in the saturation extract following the methodology of Richards (1954), and the data are shown in Table 2. It can be observed that, even with leaching fraction of 0.20, substrate salinity increased from 0.82 dS m^-1 at the beginning of the experiment to 1.65 and 3.4 dS m^-1 at the end of the experiment, on average, due to the irrigation with saline waters of 0.6 and 2.4 dS m^-1, respectively (Tables 1 and 2).

Table 2
Chemical characteristics of the substrate used in the production of Carica papaya seedlings at the end of the experiment

Water salinity (dS m-1)	Phytohormones	ECse (dS m-1)	pHse
0.6	Auxin	1.65	6.05
0.6	Cytokinin	1.46	7.08
0.6	Gibberellin	1.75	6.86
Mean	-----------	1.65	6.66
2.4	Auxin	3.54	7.11
2.4	Cytokinin	3.03	6.60
2.4	Gibberellin	3.64	6.89
Mean	------------	3.40	6.87

The obtained data were evaluated by analysis of variance (ANOVA) based on the significance of F test (p ≤ 0.05). In cases of significance, a test of means (Tukey, p ≤ 0.05) was carried out for the factor salt concentrations in the water and types of phytohormones, whereas a regression analysis was performed for the factor concentrations of phytohormones, using the software SISVAR 5.6 (Ferreira, 2011).

Results and Discussion

There was significant interaction (p ≤ 0.05) between the factors salinity, types of phytohormones and concentrations of phytohormones on survival percentage at 25 DAS. The double interactions between salinity and types of phytohormones and between types of phytohormones and their concentrations had significant effects (p ≤ 0.05) on survival percentage at 20 DAS and total dry matter. Total dry matter was also significantly affected (p ≤ 0.05) by the interaction between water salinity and phytohormone concentrations (Table 3).

Table 3
Summary of analysis of variance for survival percentage (SV) at 20 DAS and at 25 DAS, and total dry matter (TDM) of Carica papaya plants under salt stress and exogenous application of different concentrations and types of phytohormones

Sources of variation	DF	Mean square
		SV 20 days	SV 25 days	TDM (mg)
		(%)
Block	2	310.06ns	160.54ns	5.91ns
Sal	1	65809.95*	47476.69*	4765.49*
Phyt	2	1569.91*	43892.69*	1184.84*
Conc	4	697.41*	543.41*	19.73ns
Sal × Phyt	2	829.47*	12796.02*	70.82*
Sal × Conc	3	204.05ns	543.41*	60.16*
Phyt × Conc	8	503.10*	228.67*	46.25*
Sal × Phyt × Conc	8	195.00ns	228.67*	33.84ns
Error	58	130.20	96.58	21.41
CV (%)	-	15.96	22.49	16.32
Mean	-	71.47	43.70	28.35

• Sal - Salinity levels; Phyt - Phytohormones; Conc - Concentrations; DF - Degrees of freedom; CV - Coefficient of variation; ^ns Not significant and * - Significant at p ≤ 0.05

Considering the effect of the interaction between water salinity levels and types of phytohormone on the survival percentage at 20 DAS, it can be observed that the increase in water salinity from 0.6 to 2.4 dS m^-1 led to the death of approximately 50% of C. papaya plants (Figure 1A). Irrigation with high-salinity water increased substrate salinity from 0.82 to 3.4 dS m^-1 and this increment is due to the small volume of substrate, which was easily salinized to critical levels for the C. papaya crop. According to Sá et al. (2013a), C. papaya plants are highly sensitive to water electrical conductivity above 2.0 dS m^-1 in the initial growth stage. However, plants receiving exogenous application of gibberellin obtained higher survival percentage compared with those in the other treatments, with 57% of survival (Figure 1A).

Figure 1
Survival percentage of Carica papaya plants at 20 days after application of treatments: interaction between salinity and types of phytohormones (A) and interaction between types of phytohormones and concentrations of phytohormones (B)

In the interaction between types of phytohormones and their concentrations, there was no significant fit of survival percentage at the levels of gibberellin, and a mean survival of 78.89% was found at 20 days after treatment application (Figure 1B). Auxin application up to concentration of 42.3 µM L^-1 increased the survival percentage of C. papaya plants by 9.7% (Figure 1B). Exogenous application of cytokinin above the estimated concentration of 6.2 µM L^-1 reduced the survival percentage of C. papaya plants, reaching values lower than 40% of living plants at 20 days after application of 50.0 µM L^-1 of cytokinin, applied in the form of CPPU (Figure 1B).

For survival percentage at 10 days after treatment application, it can be noted that the increase in water salinity from 0.6 to 2.4 dS m^-1 caused death of 100% of C. papaya plants which received exogenous application of phytohormone (Table 4). The effect of salt stress on C. papaya plants was progressively intensified by irrigation with high-salinity water, reaching levels that are unviable for this species.

Table 4
Test of means for survival percentage of Carica papaya plants at 25 days after being subjected to salt stress and exogenous applications of different concentrations and types of phytohormones

Survival at 25 days (%)
Phytohormones	Concentrations (µM L-1)	Water salinity (dS m-1)
		0.6	2.4
Auxin	0.0	100.0 xAa	0.0 yAb
	12.5	100.0 xAa	22.2 yBab
	25.0	100.0 xAa	22.2 yBab
	37.5	100.0 xAa	33.3 yBa
	50.0	100.0 xAa	22.2 yAab
Cytokinin	0.0	96.0 xAa	0.0 yAa
	12.5	0.0 xBb	0.0 xCa
	25.0	0.0 xBb	0.0 xCa
	37.5	0.0 xBb	0.0 xCa
	50.0	0.0 xBb	0.0 xBa
Gibberellin	0.0	100.0 xAa	0.0 yAb
	12.5	100.0 xAa	44.4 yAb
	25.0	100.0 xAa	44.4 yAb
	37.5	100.0 xAa	77.8 yAa
	50.0	100.0 xAa	22.2 yAb

• ** x and y letters in the row compare the interaction of salinity for each phytohormone x concentrations; uppercase letters A, B and C in the column compare the interaction of phytohormones at each salinity x concentrations; lowercase letters a, b and c in the column compare concentrations at each salinity x phytohormones, by Tukey test at p ≤ 0.05

On the other hand, exogenous application of 37.5 µM L^-1 of auxin and particularly gibberellin were efficient at maintaining the vigor and tolerance of C. papaya plants to the salt stress, leading to survival percentages of 33.3 and 77.8%, respectively (Table 4).

Exogenous application of cytokinin within the range used here (12.5 to 50.0 µM L^-1) is not viable for C. papaya, as 100% of the plants died even in the absence of salt stress. Since cytokinins are responsible for regulating the rates of cell division and expansion (Taiz et al., 2015) and considering that plants in the present study under exogenous application of cytokinin tripled their size in the first two days after phytohormone application, there may have been an inordinate synthesis and expansion of cells, which may have led to excessive absorption of water and salts from the substrate, causing death.

In addition, lower concentration of salts was observed in the substrate cultivated with plants under cytokinin application, probably due to the absorption by plants (Table 2).

Thus, absorption and excessive accumulation of salts in the tissues consequently led to ionic imbalance at levels which caused plant death. Absorption and excessive accumulation of salts can also be related to the capacity and/or function of cytokinins to facilitate the absorption of nutrients by the plant (Taiz et al., 2015).

Auxins and cytokinins are different from the other phytohormones because they are signaling agents necessary to make metabolism viable (Taiz et al., 2015). Thus, any mutation or external factor which limits their synthesis will lead to plant death, as observed in plants that did not receive exogenous application of these phytohormones in the presence of salt stress (Table 4). On the other hand, the synthesis of gibberellins is conditioned by the genetic factor, which is greatly influenced by the environmental factor (Taiz et al., 2015), the salt stress in the present study.

Thus, since plant metabolism maintains cytokinin at satisfactorily concentrations, the exogenous application of this phytohormone (high concentrations) caused physiological disorders in C. papaya plants, including toxicity (Table 4). Smaller damage was observed in plants under exogenous application of auxin, compared with those under exogenous application of cytokinin (Table 4). Although plant metabolism maintenance is also conditioned on optimal levels of auxin in the tissues, exogenous application of auxin may signal and stimulate the synthesis of gibberellins (Taiz et al., 2015), thus causing less deleterious effects on the metabolism of C. papaya plants, in comparison with cytokinins (Table 4).

In a study by Javid et al. (2011), it was reported that exogenous application of indole-3-acetic acid (auxin) and kinetin (cytokinin) increased the production and concentration of starch in rice cultivars subjected to salt stress, in comparison to the control. Exogenous application of auxins and cytokinins probably promotes better responses in grasses and limited responses in dicotyledons.

As already cited previously, the synthesis of gibberellins is conditioned by genetic factors, which are influenced by the environment. Therefore, it can be concluded that the synthesis of gibberellins in C. papaya plants under salt stress is limited, and the exogenous application of this phytohormone can benefit C. papaya plants by increasing their vigor and tolerance to salt stress. Xu et al. (2016), evaluating the exogenous application of gibberellic acid to mitigate salt stress in genotypes of Festuca arundinacea, found that it reduced the effect of the stress on leaf production and expansion rates. These authors also found that the exogenous application of this phytohormone stimulated the expression of four genes (xyloglucan endotransglycosylase 1 and 3, expansin A4 and A7) responsible for the increase in the tolerance of these plants to salinity.

Based on the production of total phytomass in C. papaya plants affected by the interaction between salinity and types of phytohormones, it can be observed that the exogenous application of cytokinin led to lower accumulation of dry matter compared to the other phytohormones, regardless of salinity (Figure 2A). This fact is related to the excessive absorption of water, caused by the high cell expansion rate in this treatment, which caused plants to be mainly composed of water, showing a low accumulation of photoassimilates. There was no significant difference in the dry matter accumulation between plants under exogenous applications of auxin and gibberellin (Figure 2A).

Figure 2
Total dry matter (TDM) of Carica papaya plants at 10 days after treatment application: interaction between salinity and types of phytohormones (A); interaction between salinity and concentrations of phytohormones (B) and interaction between types of phytohormones and their concentrations (C)

In general, the increase in salinity reduced the dry matter accumulation of C. papaya plants, regardless of the exogenous application of phytohormones (Figures 2A and B). The reduction in phytomass accumulation in C. papaya plants under salt stress has also been reported by Sá et al. (2013a,b; 2016). These authors attribute such reduction to the osmotic and ionic effects caused by the excessive accumulation of salts in the substrate. The osmotic and ionic effects imposed by the salt stress directly affect plant development, by either limiting water absorption or through the toxicity by specific ions, particularly sodium and chloride ones. Excessive accumulation of salts has complex effect on the physiological, nutritional and hormonal interactions of plants (Munns & Tester, 2008; Syvertsen & Garcia-Sanchez, 2014; Taiz et al., 2015).

In the interaction between salinity and concentrations of phytohormone, there was a progressive linear reduction of dry matter accumulation in plants irrigated with low-salinity water as the concentration of phytohormone increased. However, in plants irrigated with high-salinity water, a quadratic behavior was observed, and highest dry matter accumulation (23.31 mg) was found at phytohormone concentration of 27.6 µM L^-1 (Figure 2B). Cunha et al. (2016) and Souza Neta et al. (2018) observed that the use of the biostimulant Stimulate®, composed of a mixture of substances [0.005% of indolebutyric acid (auxin), 0.009% of kinetin (cytokinin) and 0.005% of gibberellic acid (gibberellin)], was not efficient at mitigating the effect of salinity on sweet corn and gherkin, and found benefits only in plants grown in the absence of salt stress. The opposite was observed in the present study, which may be related to the low concentration of phytohormones in the biostimulant evaluated by these authors.

In the interaction between types and concentrations of phytohormones, cytokinin application caused a linear reduction in dry matter accumulation, whereas the concentrations of auxin and gibberellin caused a quadratic effect, with highest dry matter accumulation at concentrations of 31.7 and 26.4 µM L^-1, respectively (Figure 2C).

It should be highlighted that the highest survival rate was found at concentration of 37.5 µM L^-1 of gibberellin, which had the greatest potential to mitigate salt stress on C. papaya plants (Table 4). Thus, the greater survival of plants at the concentration of 37.5 µM L^-1 of gibberellin under salt stress conditions is probably conditioned by a high expenditure of energy to promote ionic homeostasis, affecting the accumulation of photoassimilates.

Gibberellins play an essential role in plant growth and development, including seed germination, stem elongation, leaf expansion, production of flowers and development of fruits. In addition, exogenous application of gibberellins has been able to enhance the performance of plants under salt stress conditions (Khan et al., 2012; Iqbal & Ashraf, 2013; Chunthaburee et al., 2014). Chunthaburee et al. (2014), evaluating the pre-treatment of rice seeds with gibberellic acid subjected to salt stress, observed that exogenous application of gibberellin enhances the growth of all rice cultivars evaluated under salt stress, and the effects had greater potential in the sensitive cultivars. These authors also observed that the pre-soaking of seeds in gibberellic acid contributed to improvements in ionic homeostasis and delay in the loss of pigments, besides reinforcing the antioxidant systems, by reducing the production of H₂O₂, in rice plants exposed to salt stress.

Conclusions

1. The increase in irrigation water salinity has deleterious effects on C. papaya plants, regardless of the application of phytohormones.

2. Exogenous application of gibberellin has greater potential to mitigate salt stress in C. papaya plants in comparison to auxins and cytokinins.

3. Exogenous application of gibberellin (gibberellic acid) and auxin (indole-3-acetic acid) at concentration of 37.5 µM L^-1 mitigates the effects of salinity on C. papaya plants.

4. Exogenous application of cytokinin (N-(2-chloro-pyridyl)-N-phenylurea) at concentrations of 12.5 to 50.0 µM L^-1 is not viable for C. papaya plants.

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Publication Dates

• Publication in this collection
  02 Mar 2020
• Date of issue
  Mar 2020

History

• Received
  09 Nov 2018
• Accepted
  31 Dec 2019
• Published
  12 Feb 2020