SciELO - Scientific Electronic Library Online

vol.32 issue3Genomic rearrangements in BRCA1 and BRCA2: a literature reviewHaptoglobin gene subtypes in three Brazilian population groups of different ethnicities author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Genetics and Molecular Biology

Print version ISSN 1415-4757On-line version ISSN 1678-4685

Genet. Mol. Biol. vol.32 no.3 São Paulo  2009  Epub July 10, 2009 



Population prevalence of hereditary breast cancer phenotypes and implementation of a genetic cancer risk assessment program in southern Brazil



Edenir I. PalmeroI, II; Maira CaleffiIII; Lavínia Schüler-FacciniI, IV, V, VI; Fernanda L. RothVII; Luciane KalakunVIII; Cristina Brinkmann Oliveira NettoIV; Giovana SkonieskiIII; Juliana GiacomazziII, VII; Bernadete WeberIII; Roberto GiuglianiI, II, IV, V, VI, VII; Suzi A. CameyVIII, IX; Patricia Ashton-ProllaI, II, IV, V, VII, VII

IPrograma de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
IILaboratório de Medicina Genômica, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil
IIINúcleo Mama Porto Alegre and Associação Hospitalar Moinhos de Vento, Porto Alegre, RS, Brazil
IVServiço de Genética Médica, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil
VDepartamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
VIInstituto Nacional de Genética Médica Populacional, Porto Alegre, RS, Brazil
VIIPrograma de Pós-Graduação em Ciências Médicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
VIIIPrograma de Pós-Graduação em Epidemiologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil
IXDepartamento de Estatística, Instituto de Matemática, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

Send correspondence to




In 2004, a population-based cohort (the Núcleo Mama Porto Alegre - NMPOA Cohort) was started in Porto Alegre, southern Brazil and within that cohort, a hereditary breast cancer study was initiated, aiming to determine the prevalence of hereditary breast cancer phenotypes and evaluate acceptance of a genetic cancer risk assessment (GCRA) program. Women from that cohort who reported a positive family history of cancer were referred to GCRA. Of the 9218 women enrolled, 1286 (13.9%) reported a family history of cancer. Of the 902 women who attended GCRA, 55 (8%) had an estimated lifetime risk of breast cancer 3 20% and 214 (23.7%) had pedigrees suggestive of a breast cancer predisposition syndrome; an unexpectedly high number of these fulfilled criteria for Li-Fraumeni-like syndrome (122 families, 66.7%). The overall prevalence of a hereditary breast cancer phenotype was 6.2% (95%CI: 5.67-6.65). These findings identified a problem of significant magnitude in the region and indicate that genetic cancer risk evaluation should be undertaken in a considerable proportion of the women from this community. The large proportion of women who attended GCRA (72.3%) indicates that the program was well-accepted by the community, regardless of the potential cultural, economic and social barriers.

Key words: breast cancer, genetic counseling, hereditary cancer syndromes.




Latin America is formed by low-to-medium income countries with health indicators that are evolving towards patterns seen in developed nations. The extensive ethnic and cultural diversity seen in these countries reflects their history and different degrees of admixture between native and immigrant populations. Brazil is the largest and most populated country in Latin America, with ~170 million inhabitants. Although Brazil has the eighth largest gross national product in the world, the average per capita income ranks only 39th. A significant challenge in Brazil and other Latin American countries is the inclusion of new health technologies, including genetic risk assessment and testing, in health care systems that have significant budget constraints.

The Brazilian constitution guarantees the right to medical assistance to every citizen and at least 75% of the population depends almost entirely on health care provided by the government (SUS - Sistema Único de Saúde). One special SUS program designed for community-based health care is the Programa Saúde da Família (PSF or Family Health program), created in the mid-90s and based upon multidisciplinary teams composed of a physician, nurse, 1-2 nursing assistants and 4-6 lay community health workers. Each team provides primary health care for a geographically defined group of approximately 600 families. The Family Health program has expanded rapidly and currently provides health care to about half of the population (Ramalho and Silva, 2000; Brasil, Ministério da Saúde, 2003a, 2003b; Harzheim et al., 2006).

With few exceptions, departments of health at city, state and national levels do not have explicit policies for the prevention and care of people with genetic disorders. Genetic services are mainly centered in teaching hospitals of major cities and cancer genetic testing per se is not yet covered by SUS or private health insurance. In Porto Alegre, the two existing public GCRA services are located in tertiary care centers (Penchaszadeh, 2000; Llerena, 2002; Palmero et al., 2007a).

Breast cancer is a significant public health problem throughout Brazil, and is currently the first cause of cancer-related deaths in Brazilian women of all ages. Rio Grande do Sul (RS), Brazil's southernmost state, has one of the highest incidences of breast cancer in the country and an increasing breast cancer mortality rate, despite efforts to improve the coverage for breast cancer screening (Brazil, 2003b). Breast cancer is also the leading cause of death by cancer in young women (30-49 years) from this region (Cadaval Gonçalves et al., 2007). The estimated incidence for breast cancer in RS and Brazil in 2008 was 85.5 and 51.2 per 100,000, respectively (Brazilian National Cancer Institute, 2008; Brasil, Ministério da Saúde, 2003b). Recently, SUS has anticipated the recommended age at initiation for annual mammographic screening from 50 to 40 years. However, routine mammographic screening is not yet readily available.

One of the most important risk factors for breast cancer is a positive family history, and an estimated 5%-10% of all diagnosed cases is hereditary, i.e., caused by germline mutations in high penetrance predisposition genes (De la Chapelle and Peltromaki, 1998; Offit, 1998; Margolin and Lindblom, 2006). Germline mutations in the breast cancer genes BRCA1 and BRCA2 are related to an increased risk for breast, ovarian and other cancers in a syndrome known as hereditary breast and ovarian cancer (HBOC) which accounts for most cases of hereditary breast cancer (HBC) worldwide (Miki et al., 1994; Wooster et al., 1994; Peto et al., 1999; Anglian Breast Cancer Study Group, 2000; Antoniou et al., 2000, 2002). Other genes that predispose to breast cancer, such as TP53 (associated with Li-Fraumeni and Li-Fraumeni-like syndromes, LFS/LFL) (Li and Fraumeni, 1969; Birch et al., 1994; Eeles, 1995) PTEN (associated with Cowden's syndrome) (Eng, 1997) and CHEK2 (associated with hereditary breast and colon cancer syndrome, HBCC) (Meijers-Heijboer et al., 2003) have been identified and are thought to have important, albeit lower, contributions to the phenotype (Li et al., 1988; Vahteristo et al., 2002). The identification of individuals with these syndromes is important to ensure that appropriate strategies to reduce the risk of cancer are recommended to these persons and their families (Nelson et al., 2005; Guillem et al., 2006).

Little attention has been given to the identification and study of hereditary breast cancer phenotypes in the community and primary health care services (De Silva et al., 1995; Pharoah et al., 2000; Hall et al., 2001; Hoskins et al., 2006). In this context, the main purpose of this study was to assess the prevalence of a significant family history of cancer and of hereditary breast cancer phenotypes in an underserved community with high breast cancer incidence and mortality rates in southern Brazil.


Subjects and Methods

In April 2004, a large population-based cohort study (the Núcleo Mama Porto Alegre - NMPOA Cohort) was started in Porto Alegre, the capital of the southern Brazilian state of Rio Grande do Sul. The cohort intends to collect demographic, epidemiologic and risk factor data from a large sample of women 15 years old and test a model for community-based breast cancer screening for women between the ages of 40 and 69 years, as described elsewhere (Caleffi et al., 2009 Smith et al., 2006). Women > 15 years old who visited primary health care units as part of the Family Health program in seven underserved areas of Porto Alegre were included in the NMPOA cohort from April 2004 through March 2006.

Patient recruitment

The family history of breast cancer and other tumors was assessed in all of the patients included in the NMPOA cohort by using a seven question instrument (Table 1) and considered both maternal and paternal histories in first-, second- and third-degree relatives. The instrument has been validated for this population (manuscript submitted) (Ashton-Prolla P, Giacomazzi J, Schmidt AV, Roth FL, Palmero EI, Kalakun L, Aguiar E, Moreira SM, Batassini E, Belo-Reyes V, Caleffi M, Camey S; unpublished data) and was based on features associated with an increased likelihood of clinically significant BRCA mutations (Couch et al., 1997; Shattuck-Eidens et al., 1997; Srivastava et al., 2001; Frank et al., 2002; Nelson et al., 2005). Furthermore, a question about the family history of breast cancer and/or colon cancer was included because of previous evidence indicating a higher than expected prevalence of such an association based on patients followed at cancer genetics clinics in Porto Alegre (Palmero et al., 2007b).



Women above the age of 18 years old who replied positively to at least one of the questions at the primary health care unit were referred for GCRA and invited to participate in this study. Ethical approval (Protocol number 04-170, coordinating center IRB, Hospital de Clínicas de Porto Alegre) was obtained from the institutions involved and enrollment in the study required signature of informed consent. Active recruitment was initiated after six months if the patients referred by the Family Health program did not reach NMPOA. In this case, three attempts were made to schedule a visit by telephone, followed by a letter of invitation and a search for the patient by community agents. If all of these strategies failed, or if three scheduled appointments were not kept, no further contact was attempted.

Patient sample for weighted prevalence analysis

To determine the weighted prevalence of a hereditary breast cancer phenotype in the population being studied, two groups of patients were evaluated: (a) 885 unrelated women with a family history of cancer and (b) 910 unrelated women of the same cohort with no family history of cancer upon recruitment at the primary health care unit and who were invited to participate in this study during their annual mammographic screening examinations. Recruitment was done consecutively during a period of 12 months. The evaluation included an interview, an estimation of the risk of breast cancer, and registration of the family history in pedigrees of at least three generations. The presence of criteria for breast cancer predisposition syndromes was assessed by three clinical geneticists (PAP, FLR and CBON) who independently reviewed each pedigree. The group of patients referred for genetic risk evaluation was also the group used for the validation of the seven question instrument for identification of hereditary breast cancer families (Ashton-Prolla P, Giacomazzi J, Schmidt AV, Roth FL, Palmero EI, Kalakun L, Aguiar E, Moreira SM, Batassini E, Belo-Reyes V, Caleffi M, Camey S; unpublished data).

Genetic cancer risk assessment (GCRA)

Genetic evaluation included recording of the medical and family histories of each index case in detailed pedigrees, with information traced as far back (minimum of three generations) and laterally as possible and including paternal lineages. Confirmation of the family history of cancer was attempted in all cases and pathology reports, medical records and/or death certificates were obtained whenever possible (unpublished data). Estimated lifetime risks of breast cancer (ELTR) were obtained by using Claus tables and the Gail and Tyrer-Cuzick models (Gail et al., 1989; Claus et al., 1994; Domchek et al., 2003; Tyrer et al., 2005). The clinical diagnosis of Hereditary Breast and Ovarian Cancer (HBOC) Syndrome was based on the criteria of the American Society of Clinical Oncology (ASCO, 1996; Ford et al., 1998). In addition, the prior probabilities of carrying a BRCA1 or BRCA2 mutation were determined for each patient by using mutation prevalence tables and a modified Couch (Penn II) mutation probability model (Frank et al., 2002; Domchek et al., 2003, 2004). All of the pedigrees were reviewed by at least two clinical geneticists to assess the presence of criteria for LFS, LFL, HBCC or other cancer predisposition syndromes. For LFS, LFL and HBCC, previously published criteria for their clinical diagnosis were used (Li and Fraumeni, 1969; Birch et al., 1994; Eeles, 1995; Meijers-Heijboer et al., 2003). Three breast cancer risk categories were established, namely, Risk I: < 0.2 using all three models of ELTR, Risk II: 3 0.2 with at least one of the models, and Risk III: women with a family history that fulfilled the criteria for a breast cancer predisposition syndrome.

Follow up

All of the patients were encouraged to perform monthly breast self-examinations. Women with ELTR < 0.2 (Risk I) were referred back to their primary health care unit for prospective follow-up as determined by their age and other non-genetic risk factors (monthly breast self-examination, annual clinical exam and annual mammography for women 40-69 years old). Patients with ELTR > 0.2 (Risk II) and those who fulfilled criteria for hereditary breast cancer syndrome (Risk III) were referred for clinical breast evaluations at 6-month intervals. In addition, for women in risk categories II and III, a recommendation was made for annual mammography intercalated with breast magnetic resonance imaging at 6-month intervals; breast ultrasound was recommended for those with very dense breasts and/or whenever clinically indicated. Women in risk category III began screening at the age of 25 years or as soon as a risk was identified. Those with an increased risk for tumors other than breast cancer were referred to tertiary care centers for inclusion in comprehensive cancer screening programs. Those with criteria for a breast cancer predisposition syndrome were offered genetic testing and the results of this investigation will be described elsewhere.

After conclusion of the genetic evaluation, written reports and a brochure with key information about the prevalence, treatment and prevention of breast cancer, as well as information about hereditary breast cancer, were mailed to all of the patients. The vast majority of the women included in this study relied exclusively upon the public health care system. Although mammographic screening and clinical follow-up are available in this system outside the cohort study described here, access to these facilities is limited and most women would not have been able to comply with the recommended screening guidelines if they had not been included in this study. Magnetic resonance imaging of the breast is not routinely available in the public health care system.

Statistical analysis

Quantitative variables were expressed as the mean ± SD, whereas categorical variables (descriptive analyses) were recorded as absolute and/or relative frequencies. The χ2 test was used to compare the distribution of the answers to the family history questionnaire between attenders and non-attenders, whereas ANOVA was used to compare the mean values of the risk estimates and the history of breast cancer among the different risk categories. Kappa coefficients were used to assess the agreement between results provided by the instrument and the estimated genetic cancer risk. The weighted prevalence of hereditary breast cancer phenotypes was calculated using the following weighting: group with a family history of cancer (885/1285) and group with no family history of cancer (910/7933). All data handling and statistical analyses were done using the statistical software package SPSS (version 14.0), and a value of p < 0.05 indicated significance.



Description of the population served by the program

The women enrolled in this study were residents of seven regions in Porto Alegre. These regions correspond to 64% of the total area of the city and ~35% of its population. All women relied almost exclusively on the Family Health Program for health care. Although specific demographic data for this population of the Family Health Program clients was unavailable, data from the most recent municipal census indicate that 49.2% of the inhabitants in these regions have < 8 years of education (5.6% are illiterate), 20% live in temporary homes and 6.5% of family providers have no income (Porto Alegre, Prefeitura Municipal 2004).

Patient recruitment

Of the 9218 women enrolled in the cohort, 1286 (13.9%) answered positively to at least one of the seven questions about a family history of cancer (Figure 1) and those above the age of 18 years old (n = 1247) were referred for GCRA. Of these, 260 (21%) did not reach NMPOA and did not respond to the active recruitment strategies within a 12 month period after inclusion in the cohort. In addition, 41 (3.3%) women scheduled an appointment three times but did not show up, and another 43 (3.4%) did not wish to participate in the study. The remaining 902 women from 829 families were compliant with the referral and attended GCRA.



The demographic data of women with a positive family history who underwent GCRA (attenders) differed significantly in some aspects from those of women who did not undergo GCRA (non-attenders). Non-attenders were generally younger, less educated, and had undergone a breast biopsy less often than attenders (p < 0.001). For most of the questions in the family history questionnaire, there was no significant difference between the proportion of positive answers between attenders and non-attenders. Exceptions to this included: (a) the question about a family history of breast and/or ovarian cancer in > 2 relatives, for which attenders provided a greater number of positive answers, and (b) the question about the combined occurrence of breast and ovarian cancer in a relative, for which non-attenders provided a greater number of positive answers (Table 1). The latter observation may reflect a bias of ascertainment with consequent deficiency in providing GCRA to some individuals with a high risk of HBOC. However, when the answers to this question (family member with multiple tumors) at the primary health care unit were compared to the family history reported by attenders during GCRA, and confirmed by review of medical records, there was low agreement between the responses observed, i.e. only one diagnosis (usually breast cancer) remained in most cases (Kappa coefficient = 0.069).

Risk assessment

Table 2 summarizes the demographic and other variables related to the estimation of lifetime risk for developing breast cancer in the 902 attenders. A significant proportion of the women were smokers and overweight or obese, as also observed in the NMPOA cohort as a whole (Caleffi et al., 2009). A high number of patients reported a positive family history for cancer in the maternal lineage: 26.1% had a family history of breast cancer only, whereas 19.8% had a family history of breast cancer and colorectal cancer (CRC). Most of the women evaluated (688, 76.3%) did not fulfill criteria for breast cancer predisposition syndromes. Among these women, the estimated lifetime risk of developing breast cancer was < 0.2 in 633 (92%) and > 0.2 in 55 (8%). The most common feature of the family history that justified GCRA in this group of patients was the presence of a relative with breast cancer who was < 50 years old (Table 3). The remaining 214 women (23.7%) from 183 families had pedigrees suggestive of breast cancer predisposition syndrome. Of these 183 families, the majority fulfilled criteria for the Li-Fraumeni-like syndrome (Figure 1). The overall weighted prevalence of a breast cancer predisposition syndrome phenotype in the sample studied was 6.2%. Twenty-five families fulfilled criteria for more than one syndrome. The 76 patients (65 families) who fulfilled criteria for HBOC syndrome had average BRCA mutation probabilities of 21.9 ± 13.9% and 25.7 ± 14.8% by the Penn II model and BRCA mutation prevalence tables, respectively. For all of the attenders who were unaffected by cancer, the ELTR estimates obtained using the Claus tables and the Gail and Tyrer-Cuzick models were 13.6%, 13.9% and 13.9%, respectively. Table 3 summarizes the family history of breast cancer and ELTR data for patients in the three risk categories.




By using a simple seven-question instrument to inquire about the family history of cancer in women from primary health care units of an underserved region in southern Brazil, we identified a significant proportion with family histories of cancer who fulfilled criteria for one of the most commonly recognized hereditary breast cancer syndromes. The overall prevalence (6.2%) was similar to that described in other population-based samples, although only a few studies have addressed this issue to date (Pharoah et al., 2000; Hughes et al., 2003; Palomaki et al., 2006). Most of the families with hereditary breast cancer phenotypes corresponded to LFL syndrome. Although there is a phenotypic overlap among the HBOC, LFS/LFL and HBCC syndromes, the high frequency of LFL pedigrees observed here is striking, especially considering that the original questionnaire used to identify these patients was not designed to screen for LFS/LFL.

Already ten years ago (Varley et al., 1999) described certain low penetrance TP53 alleles and suggested that deleterious mutations in TP53 (or related genes) may be more frequent in the population than previously estimated. In addition, several recent reports have indicated that a deleterious germline mutation in TP53, R337H, might be very prevalent and related to a founder effect in southern Brazil (Pinto et al., 2004; Achatz et al., 2007; Palmero et al., 2008). Although some of the criteria used here to diagnose the LFL phenotype were not stringent (i.e. Eeles criteria used to classify LFL phenotype) and may have little sensitivity for identifying germline TP53 mutations in other countries, the unexpectedly high number of criteria-positive patients suggests that LFL may indeed be a common phenotype for hereditary breast cancer in and around Porto Alegre. Future results from germline mutation testing should help to clarify this issue and contribute to our understanding of the applicability and discriminatory capacity of breast cancer risk estimation models, current diagnostic criteria and mutation prediction models for hereditary breast cancer syndromes in this population. If such a high prevalence of LFS/LFL syndrome is confirmed among families in this geographic area, an effort will have to be made to facilitate the identification of high-risk individuals and delineate effective cancer screening and prevention programs in these subjects.

The relatively large proportion of women (72.3%) who attended the proposed GCRA was very encouraging and comparable to that encountered in similar studies, e.g., 88% and 70% in underserved communities in the U.S.A. (Ricker et al., 2006) and Singapore (Chin et al., 2005), respectively. Furthermore, in a recent study by ONeill et al. (2006) in the U.S.A., the outcome of genetics referrals was evaluated in a group of women with estimated BRCA mutation probabilities > 10%. Within six-months after referral, 36% of the patients had undergone genetic evaluations (acceptors), 27% still intended to seek (intenders) and 36% refused such assessment (decliners). Population-based mammographic screening programs worldwide have also reported compliance rates of 61%-83% (Banks et al., 2002; O'Malley et al., 2002; Finney et al., 2006).

In this study, the number of attenders also has to be interpreted in light of the difficulties that most of these women face to seek advice, health care and cancer prevention opportunities. First, there might be a cultural difference in cancer-associated risk perception and/or a difficulty in understanding the impact of preventive interventions. The way of dealing with risk is not only influenced by risk perception but also by culture. This was clearly demonstrated by Hofstede (1997) in a landmark study in which different cultures around the world were characterized based on five parameters, including the uncertainty avoidance index (UAI). This index, which reflects the tolerability of a given society towards uncertainty and ambiguity, is higher in Brazil than in Denmark, the United Kingdom and the U.S.A. Such differences could interfere with cancer risk perception and motivation to seek GCRA. In addition, certain cultures are more fatalistic about cancer and perceive fewer benefits from screening (Russell et al., 2006). Second, there may be a knowledge barrier in understanding how preventive measures will ultimately increase quality of life (Achat et al., 2005; Ricker et al., 2006; Farmer et al., 2007). Third, in many of these women, care of themselves is often set aside because of more urgent needs, such as providing food, housing and education for their families. Fourth, it may be that these women are simply obeying and respecting a hierarchical structure in which they attend GCRA only because they are told to do so, and their cultural conditioning generally makes them follow health recommendations once they are given. Finally, there has been a historical lack of resources to ensure that adequate screening is provided in this area, even if there is evidence for a higher risk (Smith et al., 2006). Consequently, most of these women may have seen the program offered here as an opportunity to be grasped since they would otherwise face significant difficulties in following standard recommendations for breast cancer screening if they relied solely upon the public health care system.

For all of these reasons, programs such as that described here, which attempt to identify and prospectively follow women with an increased risk of cancer, need to consider the importance of patient education and social interventions (i.e., facilitate transportation, nutrition and childcare) in the difficult task of maintaining compliance to the recommended guidelines. A more detailed study of non-attenders may provide better clues on how to improve coverage for programs such as these, thereby ensuring that most high-risk patients have access to the information and preventive interventions they require.

In conclusion, the implementation of a GCRA program for an underserved community in southern Brazil revealed that the overall prevalence of a hereditary breast cancer phenotype in this population-based sample of women was 6.2%, which may be a matter of considerable concern in this region. In addition, the establishment of breast cancer risk categories allowed the identification of higher risk women who may benefit from more intensive screening. The relatively high number of women who attended GCRA sessions after an initial referral suggested that the program was well accepted by the community and is feasible, regardless of potential cultural, economic and social barriers.



The Núcleo Mama (NMPOA) Cohort, from which the patients were enrolled, is maintained by Associação Hospitalar Moinhos de Vento, in a partnership with Instituto da Mama do Rio Grande do Sul (IMAMA-RS) and the Municipal Health Agency of Porto Alegre. The authors thank Karen Barboza de Pereira, Diego Pasetto, Ana Cecília Mano de Azevedo, Ademar Bedin Júnior, Luciane Poletto Antunes, Juliana Zignani, Fávio Marcel Telis Gonzalez, Luciano Artico, Patricia Izetti Lisbôa Ribeiro, Ernestina Aguiar, Érica Batassini, Susana Mayer Moreira, Vanessa Belo Reyes and the NMPOA team for help with the recruitment, evaluation and follow-up of the patients included in this study. We also thank Drs. Juan Clinton Llerena Jr, Pierre Hainaut, Victor Penchaszadeh, Maria Isabel Waddington Achatz, Philip Kivitz and C. Kent Osborne for their stimulating discussions about the design and implementation of this program. This study was supported by grants from Susan G. Komen for the Cure (POP0403033), Fundo de Incentivo à Pesquisa (FIPE), Hospital de Clínicas de Porto Alegre (grant no. 04-170), FAPERGS (grant no. PPSUS-II 0700964) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES - PRODOC grant n. 00202/03-7). EIP was partly supported by grants from The International Agency for Research in Cancer (IARC) and CNPq (grant no. 203732/2005-7).



Achat H, Close G and Taylor R (2005) Who has regular mammograms? Effects of knowledge, beliefs, socioeconomic status, and health-related factors. Prev Med 41:312-320.         [ Links ]

Achatz MI, Olivier M, Le Calvez F, Martel-Planche G, Lopes A, Rossi BM, Ashton-Prolla P, Giugliani R, Palmero EI and Vargas FR (2007) The TP53 mutation, R337H, is associated with Li-Fraumeni and Li-Fraumeni-like syndromes in Brazilian families. Cancer Lett 245:96-102.         [ Links ]

Anglian Breast Cancer Study Group (2000) Prevalence and penetrance of BRCA1 and BRCA2 mutations in a population-based series of breast cancer cases. Br J Cancer 83:1301-1308.         [ Links ]

Antoniou AC, Gayther SA, Stratton JF, Ponder BA and Easton DF (2000) Risk models for familial ovarian and breast cancer. Genet Epidemiol 18:173-190.         [ Links ]

Antoniou AC, Pharoah PD, McMullan G, Day NE, Stratton MR, Peto J, Ponder BJ and Easton DF (2002) A comprehensive model for familial breast cancer incorporating BRCA1, BRCA2 and other genes. Br J Cancer 86:76-83.         [ Links ]

ASCO (1996) Subcommittee on genetic testing for cancer susceptibility. Statement of the American Society of Clinical Oncology: Genetic testing for cancer susceptibility. J Clin Oncol 14:1730-1736.         [ Links ]

Banks E, Beral V, Cameron R, Hogg A, Langley N, Barnes I, Bull D, Reeves G, English R, Taylor S, et al. (2002) Comparison of various characteristics of women who do and do not attend for breast cancer screening. Breast Cancer Res 4:R1.         [ Links ]

Birch JM, Hartley AL, Tricker KJ, Prosser J, Condie A, Kelsey AM, Harris M, Jones PH, Binchy A, Crowther D, et al. (1994) Prevalence and diversity of constitutional mutations in the p53 gene among Li-Fraumeni families. Cancer Res 54:1298-1304.         [ Links ]

Brasil, Ministério da Saúde (2003a) Programa Saúde da Família: Ampliando a cobertura para consolidar a mudança do modelo de Atenção Básica. Rev Bras Saúde Materno-Infantil 3:113-125.         [ Links ]

Brasil, Ministério da Saúde (2003b) Câncer no Brasil: Dados dos Registros de Base Populacional. Secretaria de Assistência à Saúde. Instituto Nacional do Câncer, Rio de Janeiro, 125 pp.         [ Links ]

Cadaval Gonçalves AT, Costa Jobim PF, Vanacor R, Nunes LN, Martins de Albuquerque I and Bozzetti MC (2007) Increase in breast cancer mortality in Southern Brazil from 1980 to 2002. Cad Saúde Pública 23:1785-1790.         [ Links ]

Caleffi M, Ribeiro RA, Duarte Filho DL, Ashton-Prolla P, Bedin Jr AJ, Skonieski GP, Zignani JM, Giacomazzi J, Franco LR, Graudenz M, et al. (2009) A model to optimize public health care and downstage breast cancer in limited-resource populations in Southern Brazil (Porto Alegre Breast Health Intervention Cohort). BMC Public Health 9:83.         [ Links ]

Chin TM, Tan SH, Lim SE, Iau P, Yong WP, Wong SW and Lee SC (2005) Acceptance, motivators, and barriers in attending breast cancer genetic counseling in Asians. Cancer Detect Prev 29:412-418.         [ Links ]

Claus EB, Risch N and Thompson D (1994) Autosomal dominant inheritance of early-onset breast cancer. Implications for risk prediction. Cancer 73:643-651.         [ Links ]

Couch FJ, DeShano ML, Blackwood MA, Calzone K, Stopfer J, Campeau L, Ganguly A, Rebbeck T and Weber BL (1997) BRCA1 mutations in women attending clinics that evaluate the risk of breast cancer. N Engl J Med 336:1409-1415.         [ Links ]

De la Chapelle A and Peltromäki P (1998) The genetics of hereditary common cancers. Curr Opin Genet Dev 8:298-303.         [ Links ]

De Silva D, Gilbert F, Needham G, Deans H, Turnpenny P and Haites N (1995) Identification of women at high genetic risk of breast cancer through the National Health Service Breast Screening Programme. J Med Genet 32:862-866.         [ Links ]

Domchek SM, Eisen A, Calzone K, Stopfer J, Blackwood A and Weber BL (2003) Application of breast cancer risk prediction models in clinical practice. J Clin Oncol 21:593-601.         [ Links ]

Domchek SM, Blackwood MA, Tweed AJ, Greshock J, Stopfer J, Stratton M, Easton D, Jablon L, Olopade O and Weber BL (2004) University of Pennsylvania BRCA1/BRCA2 prediction model. In: Abstract Presented at the Cancer Risk Prediction Models: A Workshop on Development, Evaluation, and Application. Washington, D.C.         [ Links ]

Eeles RA (1995) Germline mutations in the TP53 gene. Cancer Surv 25:101-124.         [ Links ]

Eng C (1997) Cowden syndrome. J Genet Counsel 6:181-191.         [ Links ]

Farmer D, Reddick B, D'Agostino R and Jackson SA (2007) Psychosocial correlates of mammography screening in older African American women. Oncol Nurs Forum 34:117-123.         [ Links ]

Finney MF, Tumiel-Berhalter LM, Fox C and Jaen CR (2006) Breast and cervical cancer screening for Puerto Ricans, African Americans, and non-Hispanic Whites attending inner-city family practice centers. Ethn Dis 16:994-1000.         [ Links ]

Ford D, Easton DF, Stratton M, Narod S, Goldgar D, Devilee P, Bishop DT, Weber B, Lenoir G, Chang-Claude G, et al. (1998) Genetic heterogeneity and penetrance analysis of the BRCA1 and BRCA2 genes in breast cancer families. The Breast Cancer Linkage Consortium. Am J Hum Genet 62:676-689.         [ Links ]

Frank TS, Deffenbaugh AM, Reid JE, Hulick M, Ward BE, Lingenfelter B, Gumpper KL, Scholl T, Tavtigian SV, Pruss DR, et al. (2002) Clinical characteristics of individuals with germline mutations in BRCA1 and BRCA2: Analysis of 10,000 individuals. J Clin Oncol 20:1480-1490.         [ Links ]

Gail MH, Brinton LA, Byar DP and Corler DK (1989) Projecting individualized probabilities of developing breast cancer for white females who are being examined anually. J Natl Cancer Inst 81:1879-1886.         [ Links ]

Guillem JG, Wood WC, Moley JF, Berchuck A, Karlan BY, Mutch DG, Gagel RF, Weitzel J, Morrow M, Weber BL, et al. (2006) ASCO/SSO review of current role of risk-reducing surgery in common hereditary cancer syndromes. J Clin Oncol 24:4642-4660.         [ Links ]

Hall IJ, Burke W, Coughlin S and Lee NC (2001) Population-based estimates of the prevalence of family history of cancer among women. Community Genet 4:134-142.         [ Links ]

Harzheim E, Duncan BB, Stein AT, Cunha CR, Gonçalves MR, Trindade TG, Oliveira MM and Pinto ME (2006) Quality and effectiveness of different approaches to primary care delivery in Brazil. BMC Health Serv Res 6:156.         [ Links ]

Hofstede G (1997) Cultures and Organizations. McGraw-Hill, New York, 279 pp.         [ Links ]

Hoskins KF, Zwaagstra A and Ranz M (2006) Validation of a tool for identifying women at high risk for hereditary breast cancer in population-based screening. Cancer 107:1769-1776.         [ Links ]

Hughes KS, Roche C, Campbell CT, Siegel N, Salisbury L, Chekos A, Katz MS and Edell E (2003) Prevalence of family history of breast and ovarian cancer in a single primary care practice using a self-administered questionnaire. Breast J 9:19-25.         [ Links ]

Li FP and Fraumeni Jr JF (1969) Soft-tissue sarcomas, breast cancer, and other neoplasms. A familial syndrome? Ann Intern Med 71:747-752.         [ Links ]

Li FP, Fraumeni Jr JF, Mulvihill JJ, Blattner WA, Dreyfus MG, Tucker MA and Miller RW (1988) A cancer family syndrome in twenty-four kindreds. Cancer Res 48:5358-5362.         [ Links ]

Llerena Jr JC (2002) Medical genetics, Single Brazilian Health System (SUS) and integrated aspect in health attention and care. Ciênc Saúde Coletiva 7:17-41.         [ Links ]

Margolin S and Lindblom A (2006) Familial breast cancer, underlying genes, and clinical implications: A review. Crit Rev Oncog 12:75-113.         [ Links ]

Meijers-Heijboer H, Wijnen J, Vasen H, Wasielewski M, Wagner A, Hollestelle A, Elstrodt F, van den Bos R, de Snoo A, Fat GT, et al. (2003) The CHEK2 1100delC mutation identifies families with a hereditary breast and colorectal cancer phenotype. Am J Hum Genet 72:1308-1314.         [ Links ]

Miki Y, Swenson J, Shattuck-Eidens D, Futreal PA, Harshman K, Tavtigian S, Liu Q, Cochran C, Bennett LM, Ding W, et al. (1994) A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1. Science 266:66-71.         [ Links ]

Nelson HD, Huffman LH, Fu R, Harris EL and U.S. Preventive Services Task Force (2005) Genetic risk assessment and BRCA mutation testing for breast and ovarian cancer susceptibility: Systematic evidence review for the U.S. Preventive Services Task Force. Ann Intern Med 143:362-379.         [ Links ]

O'Neill SM, Peters JA, Vogel VG, Feingold E and Rubinstein WS (2006) Referral to cancer genetic counseling: Are there stages of readiness? Am J Med Genet C - Semin Med Genet 142:221-231.         [ Links ]

O'Malley AS, Forrest CB and Mandelblatt J (2002) Adherence of low-income women to cancer screening recommendations. J Gen Intern Med 17:144-154.         [ Links ]

Offit K (1998) Clinical Cancer Genetics: Risk Counseling and Management. Wiley-Liss, New York, 420 pp.         [ Links ]

Palmero EI, Kalakun L, Schüler-Faccini L, Giugliani R, Regla Vargas F, Rocha JC and Ashton-Prolla P (2007a) Cancer genetic counseling in public health care hospitals: The experience of three Brazilian services. Community Genet 10:110-119.         [ Links ]

Palmero EI, Ashton-Prolla P, da Rocha JC, Vargas FR, Kalakun L, Blom MB, Azevedo SJ, Caleffi M, Giugliani R and Schuler-Faccini L (2007b) Clinical characterization and risk profile of individuals seeking genetic counseling for hereditary breast cancer in Brazil. J Genet Couns 16:363-371.         [ Links ]

Palmero EI, Schüler-Faccini L, Caleffi M, Achatz MI, Olivier M, Martel-Planche G, Marcel V, Aguiar E, Giacomazzi J, Ewald IP, et al. (2008) Detection of R337H, a germline TP53 mutation predisposing to multiple cancers, in asymptomatic women participating in a breast cancer screening program in Southern Brazil. Cancer Lett 261:21-25.         [ Links ]

Palomaki GE, McClain MR, Steinart K, Sifri R, LoPresti L and Haddow JE (2006) Screen-positive rates and agreement among six family history screening protocols for breast/ovarian cancer in a population-based cohort of 21- to 55-year-old women. Genet Med 8:161-168.         [ Links ]

Penchaszadeh VB (2000) Community genetics in Latin America: Challenges and perspectives. Community Genet 3:124-127.         [ Links ]

Peto J, Collins N, Barfoot R, Seals S, Warren W, Rahman N, Easton DF, Evans C, Deacon J and Stratton MR (1999) Prevalence of BRCA1 and BRCA2 gene mutations in patients with early-onset breast cancer. J Natl Cancer Inst 91:943-949.         [ Links ]

Pharoah PD, Lipscombe JM, Redman KL, Day NE, Easton DF and Ponder BA (2000) Familial predisposition to breast cancer in a British population: Implications for prevention. Eur J Cancer 36:773-779.         [ Links ]

Pinto EM, Billerbeck AE, Villares MC, Domenice S, Mendonca BB and Latronico AC (2004) Founder effect for the highly prevalent R337H mutation of tumor suppressor p53 in Brazilian patients with adrenocortical tumors. Arq Bras Endocrinol Metabol 48:647-650.         [ Links ]

Ramalho AS and Silva RB (2000) Community genetics: A new discipline and its application in Brazil. Cad Saude Publica 16:261-263.         [ Links ]

Ricker C, Lagos V, Feldman N, Hiyama S, Fuentes S, Kumar V, Gonzalez K, Palomares M, Blazer K, Lowstuter K, et al. (2006) If we build itwill they come? - Establishing a cancer genetics services clinic for an underserved predominantly latina cohort. J Genet Couns 15:505-514.         [ Links ]

Russell KM, Perkins SM, Zollinger TW and Champion VL (2006) Sociocultural context of mammography screening use. Oncol Nurs Forum 33:105-112.         [ Links ]

Shattuck-Eidens D, Oliphant A, McClure M, McBride C, Gupte J, Rubano T, Pruss D, Tavtigian SV, Teng DH, Adey N, et al. (1997) BRCA1 sequence analysis in women at high risk for susceptibility mutations. Risk factor analysis and implications for genetic testing. JAMA 278:1242-1250.         [ Links ]

Smith RA, Caleffi M, Albert US, Chen TH, Duffy SW, Franceschi D, Nystrom L and Global Summit Early Detection and Access to Care Panel (2006) Breast cancer in limited-resource countries: Early detection and access to care. Breast J 12(Suppl 1):S16-S26.         [ Links ]

Srivastava A, McKinnon W and Wood ME (2001) Risk of breast and ovarian cancer in women with strong family histories. Oncology 15:889-902.         [ Links ]

Tyrer J, Duffy SW and Cuzick J (2005) A breast cancer prediction model incorporating familial and personal risk factors. Stat Med 23:1111-1130. Erratum in: Stat Med (2005) 24:156.         [ Links ]

Vahteristo P, Bartkova J, Eerola H, Syrjakoski K, Ojala S, Kilpivaara O, Tamminen A, Kononen J, Aittomaki K, Heikkila P, et al. (2002) A CHEK2 genetic variant contributing to a substantial fraction of familial breast cancer. Am J Hum Genet 71:432-438.         [ Links ]

Varley JM, McGown G, Thorncroft M, James LA, Margison GP, Forster G, Evans DG, Harris M, Kelsy AM and Birch JM (1999) Are there low-penetrance TP53 alleles? Evidence from childhood adrenocortical tumors. Am J Hum Genet 65:995-1006.         [ Links ]

Wooster R, Neuhausen SL, Mangion J, Quirk Y, Ford D, Collins N, Nguyen K, Seal S, Tran T, Averill D, et al. (1994) Localization of a breast cancer susceptibility gene, BRCA2 to chromosome 13q12-13. Science 265:2088-2090.         [ Links ]


Internet Resources

Brazilian National Cancer Institute. (July 3, 2008).         [ Links ]

Brazilian Public Health System (SUS). (April 3, 2007).         [ Links ]

Brasil, Ministério da Saúde (2004) Departamento de Atenção Básica. Atenção Básica e a Saúde da Família, (March, 10 2007).         [ Links ]

Porto Alegre, Prefeitura Municipal (2004) Gabinete do prefeito, Secretaria do Planejamento Municipal. Mapas da inclusão e exclusão social de Porto Alegre, Brazil (March,24 2009).         [ Links ]



Send correspondence to
Patricia Ashton-Prolla
Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre
Rua Ramiro Barcelos, 2350
90035-903, Porto Alegre, RS, Brazil

Received: July 22, 2008; Accepted: December 2, 2008.



Associate Editor: Emmanuel Dias Neto

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License