Molecular forms of butyrylcholinesterase and obesity

Molecular forms of butyrylcholinesterase and obesity

Dellyana R. Boberg; Lupe Furtado-Alle; Ricardo L.R. Souza; Eleidi A. Chautard-Freire-Maia

Department of Genetics, Federal University of Paraná, Curitiba, PR, Brazil

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ABSTRACT

This study compared obese (N = 134) and unobese (N = 92) male blood donors, regarding the relative intensity (RI) and activity of different molecular forms (G1, G2, G4 and G1-ALB) of butyrylcholinesterase (BChE, EC 3.1.1.8) found in plasma, thereby searching for an association between these variables with obesity and SNPs of exons 1 and 4 of the BCHE gene. It was shown that obese and unobese individuals do not differ in the RI of each BChE band, even when classifying the sample into three genotypes of exons 1 and 4 of the BCHE gene (-116GG/539AA, -116GG/539AT, -116GA/539AT). Although the mean BChE activity of each band was significantly higher in obese than in unobese blood donors, the proportions of BChE bands were maintained, even under the metabolic stress associated to obesity, thereby leading to infer that this proportion is somehow regulated, and may therefore be important for BChE functions.

Key words: butyrylcholinesterase, body mass index, obesity, BCHE gene SNPs.

Human butyrylcholinesterase (BChE; EC 3.1.1.8) hydrolyses choline and other esters, this synthesis taking place in the liver, with posterior distribution to several parts of the organism. BChE is coded by the BCHE gene (3q26.1-q26.2) which presents four exons (Arpagaus et al., 1990), with more than 70 already-described variants (review in Souza et al., 2005b). Another type of genetic variation occurs through the interaction of products from the BCHE and CHE2 (2q33-q35) genes, this resulting in the CHE2 C5+ and CHE2 C5- phenotypes, characterized by the presence and absence of the C₅ complex, respectively (Harris et al., 1962).

BChE is found in human plasma, either in homomeric, viz., monomers (G1), dimers (G2), trimers (G3) and tetramers (G4) (Harris et al., 1962), or heteromeric forms associated with other substances, such as albumin (G1-ALB) (Masson, 1989).

Although the physiological role of BChE has not been fully elucidated, published data suggest its relationship with lipid metabolism (Kutty et al., 1977), weight (Chautard-Freire-Maia et al., 1991; Li et al., 2008) and body mass index (BMI) (Alcântara et al., 2001, 2003; Souza et al., 2005a; Furtado-Alle et al., 2008). Besides mean BChE activity tending to be higher in obese than in unobese individuals (Chautard-Freire-Maia et al., 1991; Alcântara et al., 2003; Furtado-Alle et al., 2008), BChE itself is also related to ghrelin (Kojima et al., 2001), a peptide associated with obesity (Tschöp et al., 2001), through its hydrolyzing and inactivating the latter (De Vriese et al., 2004).

The aim was to compare the relative intensity (RI) and activity of BChE bands (G1, G1-ALB, G2 and G4) in obese and control individuals, and search for an association between the activity of each band with obesity and SNPs of exons 1 and 4 of the BCHE gene. This research was approved by the Ethical Committee (CONEP; registration number 2063).

The sample comprised 134 obese (BMI > 30; mean age 36.9) and 92 unobese (< 20 £ BMI < 25; mean age 35.1) male blood donors bearing the CHE2 C5- phenotype. Individuals with any no detectable BChE band were excluded from the study. The detection of BChE bands in plasma - stored from eight to eleven years at -20 °C - was by means of electrophoresis (8 h; 100V and 11 mA; 4 °C) of plasma samples (5 µL) mixed with 7 µL of bromophenol blue before loading onto polyacrylamide gels (170 x 160 x 0.8 mm; 29:1 stock solution) of 5% (1 cm; stacking gel) and 7% (13 cm; resolving gel) concentrations in TBE 1X. The bands were revealed at 37 °C after 30 to 40 min incubation in a solution of 0.2% Fast Red TR in 200 mM sodium phosphate buffer (pH 7.1) containing 3.4% of 30 mM alpha-naphthyl acetate dissolved in acetone, the volume being completed with distilled water (1:1). A pre-run of 30 min was done before plasma loading. Data concerning plasma BChE activity (Dietz et al., 1972), as modified (Evans and Wroe, 1978), and genotypes of exons 1 (-116GG, -116GA, -116AA) and 4 (539AA, 539AT, 539TT) of the BCHE gene were obtained from the data bank of the Laboratory of Polymorphisms and Linkage (Genetics Department, UFPR), most of which part of a previous study (Furtado-Alle et al., 2008). The activity of each band was a result of the multiplication of plasma BChE activity by the RI of each BChE band detected in the polyacrylamide gel, subsequently measured by optical densitometry with KODAK 1D Image Analysis Software. Statistica for Windows (StatSoft, Inc., 5.5 version, 2000) was used for statistical analysis.

Mean BChE activity in plasma, as estimated for the present sample, was significantly higher in obese (6.51 ± 2.87) than in unobese men (4.61 ± 1.11; t = 6,949, p = 6 x 10^-11). Table 1 shows that obese and control individuals do not differ in the proportions of each BChE band, although the activity of each band is significantly higher in obese than in unobese men, with ratios varying from 1.32 (G1-ALB) to 1.63 (G1). When classified according to the more frequent genotypes, mean BChE activity in plasma is significantly higher in obese than unobese individuals, respectively: -116GG/539AA (N = 80, 6.58 ± 2.66 and N = 54, 5.02 ± 1.10; t = 4.67, p < 10^-5), -116GG/539AT (N = 20, 7.08 ± 3.91 and N = 12, 3.96 ± 1.05; t = 3.37, p < 0.005) and -116GA/539AT (N = 19, 5.22 ± 2.22 and N = 12, 3.95 ± 0.78; t = 2.26, p < 0.05). As to RI means of BChE bands, classified according to these genotypes, no significant difference between obese and control individuals was revealed through t-tests (data not shown).

The K mutation (539T) of the BCHE gene was associated with lower BChE activity (Rubinstein et al., 1978), and it was shown that the -116A SNP is preferentially found in cis combination with the 539T variant (Bartels et al., 1990). Recently, it was reported that the 539T SNP alone is not associated with decreased BChE activity, since this requires the 5' UTR -116A variant, probably through the latter affecting transcription and/or translation of the BCHE gene (Furtado-Alle et al., 2008). From the present data, it can be seen that obese and control samples, grouped by genotypes of exons 1 and 4 of the BCHE gene, revealed similar RI of each BChE band. Therefore, the reduced plasma BChE activity associated with genotype -116GA/539AT (Furtado-Alle et al., 2008) does not seem to affect the RI of the BChE molecular forms examined.

On considering the encountered similarity of RI in each BChE band in obese and control subjects, the higher mean activity of each band in the former can be attributed solely to higher BChE plasma activity. Even though this activity is higher in obese individuals and lower in the -116GA/539AT genotype than in others, from the data, it can be seen that the RI of each band is maintained, independently of obesity and the examined genotypes, suggesting that this proportion is regulated, and may therefore be important for BChE function(s).

Acknowledgments

Grants were received from CNPq, Fundação Araucária and CAPES.

Received: October 20, 2009; Accepted: March 30, 2010.

Associate Editor: Francisco Mauro Salzano

License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Publication Dates

• Publication in this collection
  23 July 2010
• Date of issue
  2010

History

• Received
  20 Oct 2009
• Accepted
  30 Mar 2010