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Sao Paulo Medical Journal

Print version ISSN 1516-3180On-line version ISSN 1806-9460

Sao Paulo Med. J. vol.113 no.5 São Paulo Sept./Oct. 1995

http://dx.doi.org/10.1590/S1516-31801995000500008 

SHORT COMMUNICATION

 

Reparation of peripheral nerves with fibrin glue prepared from snake venom. Preliminary results

 

 

Fabio Cardoso Juan; Izolete Aparecida Thomazini; Maria José Mendes Gianini; Fausto Viterbo; Elaine Toscano; Rosângela Aparecida Moraes; Benedito Barravieira

Universidade do Estado de São Paulo (UNESP) - São Paulo, Brazil

Address for correspondence

 

 


ABSTRACT

A new fibrin glue obtained from snake venom is presented, with possible utilization in various fields of medicine. The preparation procedures and tests in the reparation of peripheral nerves are described. The preliminary results were similar to the conventional fibrin glue procedure.

Uniterms: Fibrin glue. Snake venom. Peripheral nerves. Rats.


RESUMO

OBJETIVO: Os autores apresentam um novo produto denominado cola de fibrina derivada de veneno de cobra, com possível utilização em várias áreas da medicina.
MATERIAL E MÉTODOS: Descrevem seu processo de preparação e a reparação em nervo periférico.
RESULTADOS: Os resultados preliminares obtidos foram semelhantes aos da cola de fibrina convencional.


 

 

A trombim-like fraction was isolated from crude Crotalus snake venom through molecular exclusion and affinity chromatography (1). The active fractions were pooled and concentrated by dialysis (1,2,8). The pool was analyzed for protein concentration (6) and characterized by sodium-dodecil-sulfate polyacrilamide gel electrophoresis and immunoblotting (SDS-PAGE). The fibronogen source was the wet cryoprecipitate obtained from fresh human plasma through cold precipitation, containing an average of 120 mg/ 100 ml of fibrinogen (8).

The fibrin glue was obtained from the solution 1 (cryoprecipitate containing fibrinogen) (9) and the solution 2 (trombin-like snake venom fraction). The area to be glued was prepared, receiving Solution 1 as a first step, followed by application of equal volume of Solution 2 on top of it. After application, the rite was tightly in place for three minutes, so as to obtain firm adhesiveness (3,4,7,8). This procedure was tested for the reparation of sciatic nerves in Wistar rats, yielding results similar to those reported by other with conventional fibrin glue (5,7). The efficacy of the Snake Venom Glue can be evaluated through figures 1-3: the normal aspect of sciatic nerve (Fig. 1); the distal ending, after reparation by the Snake Venom fibrin Glue (Fig. 2); the number of nerve fibers is lower than in the proximal ending. When the distal ending was not repaired (Fig. 3), extensive fibrosis and absence of nerve fibers was seen. Comparing figures 2 and 3, we can conclud that the fibrin glue allowed adequate adherence and nerve regeneration.

 

 

 

 

 

 

 

ACKNOWLEDGMENTS

We thank Prof. Dr. Romeu C. Guimarães very much for the final revision of the text, Ana Maria Meluso by her precious collaboration in animal management and Mr. Silvio Garcia Manoel for photographic documentation.

 

REFERENCES

1. ALEXANDER, G.; GOOTHUSEN, J.; ZEPEDA, H. & SCHWARTZMAN, R.J. - Gyroxin, a toxin from the venom of Crotalus durissus terrificus, is a trombin-like enzyme. Toxicon 26(10):953,1988.         [ Links ]

2. BARRABIN, H.; MARTIARENA, J.L.; VIDAL, J.C. & BARRIO, A. - Isolation and characterization of gyroxin from Crotalus durissus terrificus venom. In: ROSENBERG, P. Toxins: Animals, plant and microbial. New York. Pergamon Press, 1978, p. 133.         [ Links ]

3. BAKER, J.W.; SPOTNITZ, W.D.; MATTHEW, T.L. & NOLAN. S.P. - Successful use of fibrin glue during 2 years of surgery at a University Medical Center. Am Surg 55(3):166, 1989.         [ Links ]

4. BORST, H.G.; HAUERICH, A.; WALTERBUSH, G.; MAATZ, W. & MESSNER. B. - Fibrin adhesive: An important hemostatic adjunct in cardiovascular operations: J. Thorac. Cardiovasc. Surg. 84:548, 1982.         [ Links ]

5. KLETHER. G. - Comparative evaluation of conventionally sutured and clot-sutured microsurgical anastomosis. In: SCHEIEDER, P. Microsurgery for stroke. New York, Springer-Verlag, (Ch, 19).         [ Links ]

6. LOWEY, O. H.; ROSEBROUGH, N.J.; FARR, A.L. & RANDALI, R.J. - Protein measurement with the folin phenol reagent. I Biol Chem 193:265-275, 1951.         [ Links ]

7. LUPINETTI, F.M.; STONY, W.S.; ALFORD Jr, W.C.; BUJRMS, G.R.; CLASSFORD, Jr, D.M.; PETRACECK, M.R. & THOMAS, C.S. - Cryoprecipitate-topical Trombin Glue. Initial experience in patients undergoing cardiac operations. J. Thorac Cardio-vasc. Surg. 90:502, 1985.         [ Links ]

8. MARLAND, F.S. & PIRKLE, H. - Biological activities and biochemical properties of thrombin-like enzymes from snake venoms. In: LUNDBLAD, R.L; ENTON, J.W; MANM, K.G; Chem and Biol of Trombin. Ann Arbor Science. Ann Arbor, Michigan, 1977, p 71.         [ Links ]

9. WALKER, R.H. - Blood and blood components: preparation, storage and shipment. In: Technical Manual of American Association of blood banks (A.A.B.B.), Ch 3, 10 ed., 1990, p. 47.         [ Links ]

 

 

Address for correspondence:
Fausto Viterbo
Rua José Del Farra 592
Botucatu - SP - Brasil - CEP 18603-790

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