Dear Editor,
We read with great interest the article published by Silva et al. entitled “Steatosis of indeterminate cause in pediatric group: is it a primary mitochondrial hepatopathy?”.11. Silva GH, Hessel G, Coelho KI, Escanhoela CA. Steatosis of indeterminate cause in a pediatric group: is it a primary mitochondrial hepatopathy? Sao Paulo Med J. 2011;129(4):217-23. In the article, the authors hypothesize that hepatosteatosis with unknown etiology presented by group of pediatric patients may be mitochondrial disease. They attribute their idea to mitochondrial structural abnormalities detected through electron microscopy examination. They indicate that they have not found any significant difference in mitochondrial density between the control and other groups. To explain this situation, they suggest that the normal distribution of cytosol becomes altered because of the existence of lipid vacuoles in hepatocytes.
We would like to comment on their findings. First of all, in mitochondrial disease, mitochondrial proliferation almost always occurs because of mitochondrial dysfunction. Absence of increased mitochondrial density is not compatible with mitochondrial disease. Furthermore, the most reliable indicator of mitochondrial density is the level of citrate synthase activity in the tissue.22. Reisch AS, Elpeleg O. Biochemical assays for mitochondrial activity: assays of TCA cycle enzymes and PDHc. In: Pon LA, Schon EA. Methods in cell biology. Mitochondria. 2nd edition. New York: Elsevier; 2007. p. 199-222. Secondly, mitochondrial structural alterations do not provide strong enough evidence to indicate mitochondrial disease in the absence of other evidence, because many reasons other than mitochondrial disease can cause mitochondrial structural abnormalities.33. Castanha Zanoli JC, Maioli MA, Medeiros HC, Mingatto FE. Abamectin affects the bioenergetics of liver mitochondria: A potential mechanism of hepatotoxicity. Toxicol In Vitro. 2012;26(1):51-6.,44. Naranmandura H, Xu S, Sawata T, et al. Mitochondria are the main target organelle for trivalent monomethylarsonous acid (MMA(III))-induced cytotoxicity. Chem Res Toxicol. 2011;24(7):1094-103. Thirdly, indicators for mitochondrial disease, such as lactic acid levels in blood, muscle histochemistry or mitochondrial enzyme activity were not evaluated in the article by Silva et al.11. Silva GH, Hessel G, Coelho KI, Escanhoela CA. Steatosis of indeterminate cause in a pediatric group: is it a primary mitochondrial hepatopathy? Sao Paulo Med J. 2011;129(4):217-23. At least mitochondrial deoxyribonucleic acid (mtDNA) alterations should be analyzed, because almost all mitochondrial disease involves mtDNA deletion, mtDNA depletion, mtDNA point mutation or nuclear deoxyribonucleic acid (nDNA) alterations.55. DiMauro S, Hirano M. Pathogenesis and treatment of mitochondrial disorders. Adv Exp Med Biol. 2009;652:139-70. So, in our opinion, the evidence is not strong enough to hypothesize that this group of patients might have a mild form of primary mitochondrial hepatopathy.
REFERENCES
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1Silva GH, Hessel G, Coelho KI, Escanhoela CA. Steatosis of indeterminate cause in a pediatric group: is it a primary mitochondrial hepatopathy? Sao Paulo Med J. 2011;129(4):217-23.
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2Reisch AS, Elpeleg O. Biochemical assays for mitochondrial activity: assays of TCA cycle enzymes and PDHc. In: Pon LA, Schon EA. Methods in cell biology. Mitochondria. 2nd edition. New York: Elsevier; 2007. p. 199-222.
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3Castanha Zanoli JC, Maioli MA, Medeiros HC, Mingatto FE. Abamectin affects the bioenergetics of liver mitochondria: A potential mechanism of hepatotoxicity. Toxicol In Vitro. 2012;26(1):51-6.
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4Naranmandura H, Xu S, Sawata T, et al. Mitochondria are the main target organelle for trivalent monomethylarsonous acid (MMA(III))-induced cytotoxicity. Chem Res Toxicol. 2011;24(7):1094-103.
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5DiMauro S, Hirano M. Pathogenesis and treatment of mitochondrial disorders. Adv Exp Med Biol. 2009;652:139-70.
RESPONSE TO LETTER TO THE EDITOR
We have carefully analyzed the remarks directed towards the original article “Steatosis of indeterminate cause in a pediatric group: is it a primary mitochondrial hepatopathy?”.11. Silva GH, Hessel G, Coelho KI, Escanhoela CA. Steatosis of indeterminate cause in a pediatric group: is it a primary mitochondrial hepatopathy? Sao Paulo Med J. 2011;129(4):217-23. Firstly, we would point out that the aim of our study was to evaluate and characterize “pure” steatosis in the pediatric group by means of morphological and morphometric analysis. This aim focused mainly on diagnostic characterization of the group of patients studied and not on elucidating the molecular mechanisms of the disease.
Some authors22. Sherlock S, Dooley J. Nutritional and metabolic liver diseases. In: Sherlock S, Dooley J, eds. Diseases of the liver and biliary system. 11th ed. Oxford: Blackwell Science; 2002. p. 423-52.−55. Boustany RN, Aprille JR, Halperin J, Levy H, DeLong GR. Mitochondrial cytochrome deficiency presenting as myopathy with hypotonia, external ophthalmoplegia, and lactic acidosis in an infant and as fatal hepatopathy in a second cousin. Ann Neurol. 1983;14(4):462-70. have taken the view that increased mitochondrial density, which may confer an oncocytic appearance on hepatocytes, is a major characteristic of microvesicular steatosis in non-alcoholic fatty liver disease (NAFLD). It is a possibility that increased mitochondrial density is an important causal factor of mitochondrial disease. Another indication of mitochondrial impairment is the frequent observation of megamitochondria, often showing crystalline inclusions. More recently, this has been indirectly correlated with oxidative stress in NAFLD.66. Sanyal AJ, Campbell-Sargent C, Mirshahi F, et al. Nonalcoholic steatohepatitis: association of insulin resistance and mitochondrial abnormalities. Gastroenterology. 2001;120(5):1183-92.
We agree that complementary analyses, such as determination of serum lactic acid and studies on mtDNA, make it possible to obtain more conclusive results. However, our study was retrospective, with material originating from biopsy files, which made it impossible to perform complementary analyses. Despite these limitations, we believe that the results support our conclusions, especially in relation to better characterization of mitochondrial morphological changes that are present in cases of fatty liver in children, for which causes such as metabolic diseases, obesity, undernutrition, infections and others were investigated and ruled out.
REFERENCES
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1Silva GH, Hessel G, Coelho KI, Escanhoela CA. Steatosis of indeterminate cause in a pediatric group: is it a primary mitochondrial hepatopathy? Sao Paulo Med J. 2011;129(4):217-23.
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2Sherlock S, Dooley J. Nutritional and metabolic liver diseases. In: Sherlock S, Dooley J, eds. Diseases of the liver and biliary system. 11th ed. Oxford: Blackwell Science; 2002. p. 423-52.
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3Morris AA. Mitochondrial respiratory chain disorders and the liver. Liver. 1999;19(5):357-68.
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4Naviaux RK, Nyhan WL, Barshop BA, et al. Mitochondrial DNA polymerase gamma deficiency and mtDNA depletion in a child with Alpers' syndrome. Ann Neurol. 1999;45(1):54-8.
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5Boustany RN, Aprille JR, Halperin J, Levy H, DeLong GR. Mitochondrial cytochrome deficiency presenting as myopathy with hypotonia, external ophthalmoplegia, and lactic acidosis in an infant and as fatal hepatopathy in a second cousin. Ann Neurol. 1983;14(4):462-70.
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6Sanyal AJ, Campbell-Sargent C, Mirshahi F, et al. Nonalcoholic steatohepatitis: association of insulin resistance and mitochondrial abnormalities. Gastroenterology. 2001;120(5):1183-92.
History
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Reviewed
23 Mar 2012 -
Received
23 Mar 2012 -
Accepted
2 May 2012
Publication Dates
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Publication in this collection
Mar 2013
History
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Reviewed
13 Jan 2012 -
Received
13 Jan 2012 -
Accepted
2 May 2012