SciELO - Scientific Electronic Library Online

 
vol.10 issue1Effects of organic mineral dietary supplementation on production performance and egg quality of white layers author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Brazilian Journal of Poultry Science

Print version ISSN 1516-635XOn-line version ISSN 1806-9061

Rev. Bras. Cienc. Avic. vol.10 no.1 Campinas Jan./Mar. 2008

https://doi.org/10.1590/S1516-635X2008000100010 

Studies with sanguinarine like alkaloids as feed additive in broiler diets1

 

 

Vieira SL; Berres J; Reis RN; Oyarzabal OA; Coneglian JLB; Freitas DM; Peña JEM; Torres CA

Mail Address

 

 


ABSTRACT

This research included two studies evaluating the live performance of broilers fed Sangrovit® (minimum of 1.5% sanguinarine, a quaternary benzo[c]phenanthridine alkaloid extracted from Macleaya cordata). Both studies were conducted using Ross 308 female broiler chicks. Birds were fed corn-soybean meal all-vegetable diets without growth promoters with 5 treatments and 8 replications in each study. In the first study, treatments were composed of a Negative Control without feed additive and four diets with graded increases of Sangrovit of 12.5, 25, 37.5, and 50 ppm. In the second study, chicks received a similar diet from placement to 21 days of age and subsequently were given feeds with graded reductions in crude protein (CP) as follow: a Negative Control with 19.7% CP without sanguinarine, and then 19.7, 19.2, 18.8 and 18.3% CP supplemented with Sangrovit at 20 ppm. It was demonstrated that body weight was increased when birds were fed 50 ppm of Sangrovit at 21 d when compared to the Negative Control. Also comparatively to the Negative Control, cumulative feed conversion was improved for birds fed with Sangrovit at 37.5 ppm as well as feed intake from placement to 7 days at 12.5 ppm. No differences were observed in feed intake. Birds supplemented with Sangrovit and 18.8% CP had similar body weight gain and feed intake as the Negative Control with 19.7% CP. Mortality in both studies was not correlated with the treatments. Results from both studies indicate benefits of the supplementation of Sangrovit in diets for broilers.

Keywords: Broilers, feed additive, Sangrovit®, sanguinarine.


 

 

INTRODUCTION

A great deal of controversy remains on the use of antimicrobials added to broiler feeds. Epidemiological events that result in antimicrobial resistance by bacteria are difficult to study, and several factors, including the overuse or misuse of antimicrobials in human and veterinary medicine, may intensify the severity of the problem (Shea, 2004). Growth promoters have been banned in European countries and voluntarily withdrawn by some producers in order to supply specific market demands. There has been an increase in the research on alternatives to growth promoters, including plant extracts. The use of natural antimicrobials produced from herbs and spices lends itself to more favorable acceptance by the general public as well as by countries that restrict the imports of products derived from animals fed antibiotics (Dickens & Ingram, 2001). Active compounds obtained from plants have been used for a variety of human needs for centuries; however, many commercially available additives based on natural extracts lack a definite mode of action in animal feeds.

Natural compounds extracted from plants, such as quaternary benzo[c]phenanthridine alkaloids (QBA) sanguinarine and chelerythrine, are known to have antimicrobial (Eisenberg et al., 1991; Mewton et al., 2002; Colombo & Bosisio, 1996), anti-inflammatory (Lenfeld et al., 1981; Tanaka et al., 1993), and immunomodulatory (Agarwal et al., 1991; Chaturvedi et al., 1997) effects. Sanguinarine has been incorporated into swine, bovine, and poultry diets to reduce amino acid degradation, increase feed intake, and promote growth (Tschirner, 2004; Susenberth & Wolffran, 2003). Improvements in protein retention by reducing intestinal decarboxylation of aromatic amino acids through the inhibition of L-amino acid decarboxylase (Drsata et al., 1996), and enhancement of feed intake by modulating effects on the tryptophan-serotonine pathway have also been suggested as part of their a mode of action (Mellor, 2001).

Our experiments were aimed at evaluating the live performance of broilers fed corn-soybean meal all-vegetable diets supplemented with sanguinarine like alkaloids produced from the extraction of Macleaya cordata.

 

MATERIAL AND METHODS

Two studies were conducted using steel-cage batteries (0.95 X 0.80 m). In Study 1, a titration with graded increases of Sangrovit® was used, whereas in the Study 2, Sangrovit was added at a fixed dosis of 20 ppm in feeds given from 21 to 35 days of age.

Study 1

Four hundred one-day-old Ross X Ross 308 female broiler chicks (average individual weight = 43.9 g), vaccinated for Marek's disease and infectious bronchitis were obtained from a commercial hatchery. Birds were randomly placed in 40 steel-cage batteries, with 10 birds per cage, in a continuous lighting program with feed and water provided ad libitum.

Corn-soybean meal all-vegetable mash diets without antibiotic growth promoters or anticoccidials were provided throughout the study. Feeding program consisted of starter feed from placement to 21 d, and grower feed from 21 to 35 d. Feeds had similar energy and nutrients levels after formulation according to Rostagno et al. (2005) as shown in Table 1. Treatments consisted of a Negative Control without feed additive and four graded levels of Sangrovit (12.5, 25, 37.5, and 50 ppm). Each treatment had 8 replications.

Birds were weekly weighed, and feed conversion ratio corrected for the weight of dead birds was calculated. Mortality was recorded daily. Data were analyzed using the Anova procedure of SAS (SAS, 2001). Tukey's test was used to assess differences between means, and values of P £ 0.05 were considered statistically significant. Mortality data was analyzed after arcsin transformation.

Study 2

Four hundred one-d-old Ross X Ross 308 female broiler chicks vaccinated for Marek's disease and infectious bronchitis were obtained from a commercial hatchery, and randomly placed in 40 steel-cage batteries, with 10 birds per cage. Birds were submitted to a 24-h lighting program with feed and water provided ad libitum. Broilers were fed the same feed until 21 d of age, and then individually weighed and randomly distributed with 7 birds per cage (average individual weight = 662 g).

Corn-soybean meal all-vegetable mash diets without antibiotic growth promoters or anticoccidials were provided throughout the study. Diets are shown in Table 1 and had similar energy and nutrient levels after formulation according to Rostagno et al. (2005). Treatments included a Negative Control with 19.7% crude protein (CP) without the feed additive, and diets with graded reductions of CP and supplemented with 20 ppm of Sangrovit as follows: 19.7, 19.2, 18.8, and 18.3%. Each treatment had 8 replications. Measurements and statistical analyses were performed as in Study 1.

 

RESULTS

The effects of treatments on performance parameters of studies 1 and 2 are presented in Tables 2 and 3. In both studies, mortality rate was considered normal for that time of the year and growth rate of the birds, and it was not affected by the treatments (grand means = 4.69 % in Study 1 and 1.50% in Study 2).

In study 1, body weight linearly increased at 21 d in birds fed Sangrovit (p £ 0.05). Effects were not observed in the other periods. Feed conversion ratio corrected for the weight of dead birds improved during the total experimental period when sanguinarine were added at 37.5 ppm to the feed in study 1. This response was quadratically adjusted (p £ 0.001) with the maximum response calculated as (-b/2a) at 32.84 ppm. Feed intake of birds fed Sangrovit improved during the first week,, whereas differences were not observed (p £ 0.05) during the other periods.

In Study 2, body weight and feed intake were not influenced by dietary protein reductions; however, feed conversion ration was negatively affected from 21 to 28 and from 21 to 35 d (Table 3). Mean differences were observed only between treatments with extremes in dietary protein, but the effects of the graded reductions in protein were linear and indicate that loss in feed conversion ratio caused by these reduction were not be recovered with the addition of Sangrovit at 20 ppm.

 

DISCUSSION

The results from Study 1 indicate a feed conversion ratio benefit with the use of sangrovit in broilers as previously shown in swine (Tschirner et al., 2003). It has been largely accepted that sub-therapeutic doses of antimicrobials are beneficial in intensive animal production systems. However, Niewold (2007) recently suggested that expected growth promoter effects of antimicrobials on animals are in fact mediated by anti-inflammatory mechanisms. Previous research studies on QBAs suggested a combination of antimicrobial and anti-inflammatory effects as an explanation for their benefits on animal performance. QBAs have antimicrobial activity (Lenfeld et al., 1981), and minimum inhibitory concentrations of sanguinarine against several bacteria were found in the oral cavity of humans (Dzink & Socransky, 1985). Many of these bacteria belong to species commonly found in the gastrointestinal tract of chickens. Therefore, sanguinarine effects against them are expected by analogy. In parallel, it is possible to speculate that sanguinarine activity as an anti-inflammatory substance may play a role in the partial improvements obtained in the broilers of Study 1. For instance, anti-inflammatory activity has been largely demonstrated with various QBA's, including sanguinarine (Lenfeld et al., 1981; Agarwal et al., 1991). In pig intestines, this activity is expected to be caused mostly by contact, since the bulk of these alkaloids are excreted in the feces, and only a very small proportion is absorbed (Kosina et al., 2004).

Sanguinarine has been shown to reduce intestinal decarboxylation of aromatic amino acids through the inhibition of L-amino acid decarboxylase in swine (Drsata et al., 1996). The rationale for reducing dietary protein in Study 2 was to investigate a possible similar effect in broilers. The performance obtained with the graded CP reductions appeared to be sustained to the level of 18.78% CP for feed conversion rate and of 18.32% for body weight and feed intake. However, protein retention measurements should be conducted to conclude that the impact of the reduction in protein was counteracted by the supplementation of the additive.

If Niewold's (2007) theory is correct, the search for alternatives to traditional antibiotic growth promoters does not necessarily mean a search for alternative antimicrobials. Sanguinarine, as some of other QBAs, has several interesting characteristics as a feed additive for broilers. Most of these characteristics support the benefits in body weight and feed conversion ratio observed in this study.

 

CONCLUSIONS

1. Dietary supplementation with sangrovit significantly improved body weight at 21 days, feed conversion ratio at 35 days, and feed intake at 7 days of age. While body weight was linearly affected by 50 ppm of Sangrovit at 21 days, feed conversion ratio at 35 days was maximized at the supplementation level of 32.8 ppm of Sangrovit.

2. Body weight and feed intake were not affected when broilers were fed diets with graded reductions of crude protein; however, feed conversion ratio was negatively affected when with protein levels of 18.78% and lower.

 

REFERENCES

Agarwal S, Reynolds MA, Pou S, Peterson DE, Charon JA, Suzuki JB. The effect of sanguinarine on human peripheral blood neutrophil viability and functions. Oral Microbiology and Immunology 1991; 6:51-61.         [ Links ]

Chaturverdi MM, Kumar A, Darnay BG, Chainy GBN, Agarwal S, Aggarwal BB. Sanguinarine (pseudochelerythrine) is a potent inhibitor of NP-kappa B activation, I kappa B alpha phosphorylation, and degradation. Journal of Biological Chemistry 1997; 272(48): 30129-30134.         [ Links ]

Colombo ML, Bosisio E. Pharmacological activities of Chelidonium majus L. (Papaveraceae). Pharmacological Research 1996; 33:127-134.         [ Links ]

Dickens JA, Ingram KD. Efficacy of an Herbal Extract, at Various Concentrations, on the Microbiological Quality of Broiler Carcasses After Simulated Chilling. Journal Applied Poultry Research 2001; 10:194-198.         [ Links ]

Drsata J, Ulrichova J, Walterova D. Sanguinarine and chelerythrine as inhibitor of aromatic amino acid descarboxylase. Journal of Enzime Inhibition 1996; 10:231-237.         [ Links ]

Dzink JL, Socransky SS. Comparative in vitro activity of sanguinarine against oral microbial isolates. Antimicrobial Agents and Chemotherapy 1985; 27:663-665.         [ Links ]

Eisenberg AD, Young DA, Fan-Hsu J, Spitz LM. Interaction of sanguinarine and zinc on oral streptococci and Actinomyces species. Caries Research 1991; 25:185-190.         [ Links ]

Kosina P, Walterová D, Ulrichová J, Lichnovský V, Stiborová M, Rýdlová H, Vicar J, Krecman V, Brabec MJ, Simánek V. Sanguinarine and chelerythrine: assessment of safety on pigs in ninety days feeding experiment. Food and Chemical Toxicology 2004; 42:85-91.         [ Links ]

Lenfeld J, Kroutil M, Marsálek E, Slavík J, Preininger V, Simánek V. Antiinflammatory activity of quaternary benzophenanthridine alkaloids from Chelidonium majus. Planta Medica 1981; 43:161-165.         [ Links ]

Mellor S. Natural appetisers from plants. Feed Mix 2001; 9:29-31.         [ Links ]

Newton SM, Lau C, Gurcha SS, Besra GS, Wright CW. The evaluation of forty-three plant species for in vitro antimycobacterial activities; isolation of active constituents from Psoralea corylifolia and Sanguinaria canadensis. Journal Ethnopharmacology 2002; 79:57-67.         [ Links ]

Niewold TA. The non antibiotic anti-inflammatory effect of antimicrobial growth promoters, the real mode of action? A hypothesis. Poultry Science 2007; 86:605-609.         [ Links ]

Rostagno HS, Albino LFT, Donzele JL, Gomes PC, Oliveira RF de, Lopes DC, Ferreira AS, Barreto SLT. Tabelas brasileiras para aves e suínos. composição de alimentos e exigências nutricionais. 2nd ed. Viçosa: UFV; 2005.         [ Links ]

SAS Institute. SAS/STAT User's guide: release 8.0. Cary; 2001.         [ Links ]

Shea KM. Nontherapeutic use of antimicrobial agents in animal agriculture: implications for pediatrics. Pediatrics 2004; 114:862-868.         [ Links ]

Tschirner K, Susenbeth A, Wolffram S. Influence of Sangrovit® supplementation on nitrogen balance and feed intake in growing pigs. 9th Symposium Vitamins and Additives in the Nutrition of Man and Animal; 2003 sep. 24-25; Jena: Friedrich Schiller University; 2003. p.45         [ Links ]

Tanaka T, Metori K, Mineo S, Hirotani M, Furuya T, Kobayashi S. Inhibitory effects of berberine-type alkaloids on elastase. Planta Medica 1993; 59:200-202.         [ Links ]

Tschirner K. Untersuchungen zur wirksamkeit und zum nachweis des pflanzlichen alkaloids sanguinarin beim schwein [dissertation]. Kiel: Christian Albrechts Universität Kiel; 2004.         [ Links ]

 

 

Mail Address
Departamento de Zootecnia
Universidade Federal do Rio Grande do Sul
Av. Bento Gonçalves, 7712
91.540-000. Porto Alegre, RS, Brazil.
Phone/FAX: 55 51 3308 6048
E-mail: slvieira@ufrgs.br

Arrived: December / 2007
Approved: February / 2008

 

 

1 Presented at Conferência APINCO de Ciência e Tecnologia Avícolas, Santos - SP, p.48-49, 2006

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License