Abstracts
Peroxidase, present in peroxisomes and lysosomes, belongs to the oxidases and acts as a catalyst for hydrogen peroxide (H2O2) and is later decomposed by oxidation of cosubstrates thereby preventing cell damage.(1) The peroxidase technique(2) was applied to blood smears of Phrynops geoffroanus and the results compared with human blood to evaluate the activity and control of the reaction. The human blood film showed markings in neutrophils and phagocytes with many lysosomes and peroxisomes (Figure 1). In blood smears of Phrynops geoffroanus, the markings were on the basophils (Figure 2), that represent 10% to 25% of leukocytes of turtles and have a large number of cytoplasmatic granules(3) suggesting the presence of large amounts of enzymes and organelles such as lysosomes and peroxisomes, possibly associated with their participation in immune reactions. Peroxidase activity is the body's response to harmful environmental actions and serves as a biological marker.
Phrynops geoffroanus; Peroxidase; Basophils
As peroxidases, presentes nos peroxissomos e lisossomos, pertencem às oxidases e atuam como catalítico para o peróxido de hidrogênio (H2O2), posteriormente decomposto pela oxidação de cossubstratos, evitando danos celulares.(¹) Foi aplicada a técnica da peroxidase(2) em esfregaços sanguíneos de Phrynops geoffroanus, comparando com sangue humano, para avaliação da atividade e controle da reação. O esfregaço sanguíneo humano apresentou marcações em neutrófilos, fagócitos com muitos lisossomos e peroxissomos (Figura 1). Nos esfregaços sanguíneos de Phrynops geoffroanus, as marcações apresentaram-se nos basófilos (Figura 2), que representam de 10% a 25% dos leucócitos de quelônios e possuem grande número de granulações citoplasmáticas,(3) sugerindo a presença de grande quantidade de enzimas e organelas como lisossomos e peroxissomos, possivelmente associadas a sua participação em reações imunes. A atividade peroxidásica representa resposta do organismo a ações ambientais danosas, servindo como marcador biológico.
Phrynops geoffroanus; Peroxidase; Basófilos
IMAGES IN CLINICAL HEMATOLOGY
IPost Graduation Program in Genetics, Turtle Study Center, Instituto de Biociências, Letras e Ciências Exatas - Universidade Estadual Paulista "Julio de Mesquita Filho" - UNESP, São José do Rio Preto (SP), Brazil
IIBiology Department, Turtle Study Center, Instituto de Biociências, Letras e Ciências Exatas - Universidade Estadual Paulista "Julio de Mesquita Filho" - UNESP, São José do Rio Preto (SP), Brazil
Correspondence
ABSTRACT
Peroxidase, present in peroxisomes and lysosomes, belongs to the oxidases and acts as a catalyst for hydrogen peroxide (H2O2) and is later decomposed by oxidation of cosubstrates thereby preventing cell damage.(1) The peroxidase technique(2) was applied to blood smears of Phrynops geoffroanus and the results compared with human blood to evaluate the activity and control of the reaction. The human blood film showed markings in neutrophils and phagocytes with many lysosomes and peroxisomes (Figure 1). In blood smears of Phrynops geoffroanus, the markings were on the basophils (Figure 2), that represent 10% to 25% of leukocytes of turtles and have a large number of cytoplasmatic granules(3) suggesting the presence of large amounts of enzymes and organelles such as lysosomes and peroxisomes, possibly associated with their participation in immune reactions. Peroxidase activity is the body's response to harmful environmental actions and serves as a biological marker.
Keywords: Phrynops geoffroanus; Peroxidase/cytochemistry; Basophils
References
- 1. de Azeredo-Oliveira MT, Mello ML. Peroxidase activity in Malpighian tubules of Triatoma infestans Klug. Cytobios. 1998;93(373):83-92.
- 2. Lison L. Histochemie et cytochimie animals: Principes et méthods. 3a ed. Paris: Gauthier-Villars; 1960.
- 3. Goulart CE. Herpetologia, herpetocultura e medicina de répteis. Rio de Janeiro: L.F. Livros de Veterinária; 2004. p.21-56, 99-108, 131-44.
Peroxidase activity in the basophils of Phrynops geoffroanus (Testudines: Chelidae)
Publication Dates
-
Publication in this collection
01 Feb 2012 -
Date of issue
2010
