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Brazilian Archives of Biology and Technology

Print version ISSN 1516-8913On-line version ISSN 1678-4324

Braz. arch. biol. technol. vol.47 no.4 Curitiba Aug. 2004

http://dx.doi.org/10.1590/S1516-89132004000400005 

AGRICULTURE, AGRIBUSINESS AND BIOTECHNOLOGY

 

Responses of Neomegalotomus parvus (Hemiptera: Alydidae) to color and male-lured traps

 

 

Maurício Ursi VenturaI, *; Antônio Ricardo PanizziII

IDepartamento de Agronomia; Universidade Estadual de Londrina; Campus Universitário; C. P. 6001; 86051-970; Londrina - PR - Brazil
IIEMBRAPA Soja; C. P.231; 86001-970; Londrina - PR - Brazil

 

 


ABSTRACT

In 1998, the relative responses of Neomegalotomus parvus (Westwood) (Hemiptera: Alydidae) to colors in traps were assessed in the field. Colors were printed in white alkaline paper and covered with transparent plastic. Printed colors were black, green, yellow, red and blue. Treatments also included white paper and aluminum foil. Green traps captured more bugs than the other traps (except yellow). Yellow traps caught significantly more N. parvus males than blue, black and aluminum traps. White, red and blue traps captured significantly more N. parvus males than black traps. In 1999, the same treatments were used and additionally included the factor presence (X absence) of males in cages in the traps. Four males were confined in the cages together with pigeon pea seeds and water. Control traps received only pigeon pea seeds and water. No responses were found for color comparisons. Male-lured traps captured significantly more males than control traps.

Key words: Insecta, semiochemical, color responses, aggregation pheromone


RESUMO

Em 1998, as respostas relativas de Neomegalotomus parvus (Westwood) (Hemiptera: Alydidae) a cores em armadilhas foram avaliadas no campo. As cores foram impressas (preto, verde, amarelo, vermelho e azul), em papel alcalino branco e coberto com plástico transparente. Também se incluiu papel branco e folha de alumínio, como tratamentos. Armadilhas verdes capturaram mais insetos que outras armadilhas (exceto amarelo). Armadilhas amarelas capturaram significativamente mais machos de N. parvus do que armadilhas azuis, pretas e alumínio. Armadilhas brancas, vermelhas e azuis capturaram significativamente mais machos de N. parvus do que armadilhas pretas. Em 1999, os mesmos tratamentos foram usados e também se incluiu fator presença (X ausência) de machos em gaiolas nas armadilhas. Quatro machos foram confinados nas gaiolas junto com sementes de guandu e água. Testemunhas receberam somente sementes de guandu e água. Não foram obtidas respostas significativas à cores. Armadilhas com machos capturaram significativamente mais machos que armadilhas testemunhas.


 

 

INTRODUCTION

Perception of form, pattern, contrast, intensity, movement, distance, color and polarized light were demonstrated in insect species (Romoser, 1981). Hue, saturation and intensity are attributes that define color (Moerick, 1969) to which insect responses have been demonstrated (Vernon, 1986). Visual characteristics together with odor and texture are components of the primary attraction. Studies concerning on responses to colors are particularly abundant on Homoptera (e.g., Meyerdirk and Oldfield, 1985; Vaishampayan et al., 1975). For Heteroptera, we did not find reports on positive responses. Lygus lineolaris (P. de B.) adults are not visually oriented toward the hue of apple structures on which they feed or rest (Prokopy and Owens, 1978).

Insects also may reach host plants using cues of coespecifics. This is the case of insects that produce aggregation pheromones, what is classified as secondary attraction (Scholz et al., 1997). Production of aggregation pheromone was found in the alydid Riptortus clavatus Thunb. (Numata et al., 1990; Leal et al., 1994).

As a component of the primary attraction, responses of N. parvus (Weswood) to colors traps were assessed in the field. In addition, we investigated the responses of adults to traps containing males, as an evidence of the production of the aggregation pheromone (secondary selection).

 

MATERIAL AND METHODS

Traps were placed in the field at the Embrapa Farm (Londrina County, northern Paraná State, Brazil (latitude 23º S and longitude 51º W).

Responses to Colored Traps

Traps were plastic vessels (20 X 39 X 5 cm) containing water and drops of neutral detergent. Vessels were covered with white alkaline paper (RIPAX Laser 75, Ripasa S.A. Celulose e Papel, Limeira, SP, Brazil), printed with five colors (Table 1). Printed paper was covered with transparent plastic Com-Tact (Vulcan, São Paulo, SP, Brazil). Printed colors were from Microsoft Power Point (Microsoft Corporation).

Colors were also printed on transparency film to determine wavelengths in a digital spectrophotometer (Micronal 834211). Treatments also included aluminum foil and alkaline white paper. Traps were placed on the ground [Oxisol, color 2.5 YR 3 (Munsell Soil Color Charts 1988)] in 14 days, from August 8, 1998.

Distance between traps was 5 m. Traps were placed in the field at 5:00 PM, and removed after 24 h. Analysis of variance (ANOVA) was used to compare individual means (SAS Institute 1989). Means and standard errors of means presented are untransformed.

Responses to Colors X Male Lured Traps

A cage made of plastic film vial (cylindrical 3 cm diameter X 5 cm height) placed hung in a nylon line in a inverted "L shape" stake close the water surface. Cages had 4 holes (1 X 3 cm), closed with nylon screen. Four males were confined in the cages together with pigeon pea seeds and water. Control traps received only pigeon pea seeds and water. For each color in the previous experiment, we used a male-lured trap and control one in a split-plot design. Traps were placed in the field for 6 days, from September 5, 1999. Analyses of variance (ANOVA) was used to compare individual means (SAS Institute 1989). Means and standard errors of means presented are untransformed.

 

RESULTS AND DISCUSSION

Responses to Colored Traps

Wavelengths of printed colors are shown in the Fig. 1. Responses were assessed only for males due to reduced number of females caught (5.8% of total). Green traps captured more bugs than the other traps (except yellow) (Table 2). Yellow traps caught significantly more N. parvus males than blue, black and aluminum traps. Green and yellow traps probably mimic plant foliage confirming general pattern of attraction for herbivorous insects (Prokopy and Owens, 1983). White, red and blue traps captured significantly more N. parvus males than black probably due to greater contrast with the soil surface. Low captures in aluminum traps corroborated information of the mimicry of sun polarized light determining lack of attraction (Prokopy and Owens, 1978). Contrast perception was reported in the host plant selection for the fruit fly Rhagoletis pomonella (Walsh.) (Tephritidae) (Prokopy and Owens, 1978).

 

 

Responses to Colors X Male Lured traps

No significant differences were detected among colors (Tables 3 and 4). In this case only 6 replicates were used while in the previous 14 (Table 4). We observed that sometimes insects landed in the trap edges but did not fall in the water. These insects could exert effect on coespecifics what could be particularly true for N. parvus due to its aggregation behavior (Ventura et al., 2000; Ventura and Panizzi, 2003). The probable emanation of defensive secretions when insects fell in the water and even after dead can also repeal insects (Millar, J. Personal communication for M.U.Ventura).

Interaction between colors and male-lured traps was not significant (p < 5%) (Table 3). Interaction was significant just at 10% level. In treatments with low color captures, lured traps (except aluminum) tended to equilibrate level of captures with the more attractive color traps (green and yellow) (Fig. 2). More attractive colors could mask responses to volatile attractants (Andersen and Wilkin 1986).

Male-lured traps captured significantly more beetles than control traps (Tables 3 and 5). The probable explanation included the production of pheromone by N. parvus. This pheromone also may be related with gregarious behavior (Ventura and Panizzi, 2003). Males of Riptortus clavatus Thunb. also attract coespecifics (Numata et al. 1990) due to the aggregation pheromone (Leal et al. 1994). Hexyl butyrate, hexyl valerate and hexyl hexanoate were extracted from N. parvus and the mixture was attractive for adults (mostly males) (W.S. Leal and A.R. Panizzi, unpublished data). Captures of N. parvus females were reduced (3.5%) in opposition to females of R. clavatus which were caught in greater number than males (Numata et al. 1990). In the field, males of N. parvus land on people and objects. Males of Dysdercus bimaculatus Stal (Pyrrhocoridae) are the principal colonizers in new seeds sites (Aldrich 1995). Probably the exploratory behavior of N. parvus males determine greater trap captures.

In conclusion, males were attracted differently to color traps and more attracted by male-lured traps than control traps.

 

ACKNOWLEDGEMENTS

We thank J. J. Silva and E. D. Machado for logistical assistance.

 

REFERENCES

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Ventura, M. U. and Panizzi, A. R. (2003), Population Dynamics, Gregarious Behavior and Oviposition Preference of Neomegalotomus parvus (Westwood) (Hemiptera: Alydidae). Braz. Arch. Biol. Techn., 46, 33-39.        [ Links ]

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Received: December 17, 2002
Revised: June 09, 2003
Accepted: November 18, 2003

 

 

* Author for correspondence

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