Acute myonecrosis in horse caused by Clostridium novyi type A

Acute myonecrosis in horse caused by Clostridium novyi type A

Luana D'avila Farias^I; Marcos Da Silva Azevedo^II; Maria Elisa Trost^III; Flávio Desessards De La Côrte^II; Luiz Francisco Irigoyen^III; Agueda Castagna de Vargas^I

^IDepartamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

^IIDepartamento de Clínica de Grandes Animais, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

^IIILaboratório de Patologia Veterinária, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

^{Send correspondence}

ABSTRACT

The objective of this study was to describe the first report involving a case of equine acute myonecrosis caused by C. novyi type A with an emphasis on clinical signs, the pathological and bacteriological analysis, and molecular identification of the microorganisms as the key of the definitive diagnosis.

Key words: malignant edema, Clostridium novyi, equine, anaerobe infection, 16SrRNA gene.

Clostridial myositis, also called malignant edema, is a disease that causes severe necrotizing soft tissue infections. In domestic animals, these infections are most common in cattle and sheep, with C. septicum. C. chauvoei. C. novyi and C. perfringens being the most frequently isolated causative pathogens (Songer, 2010).

Equine species are less commonly affected by malignant edema; however, there are published veterinary reports that describe muscle infections caused by the following Clostridium species: C. fallax, C. septicum, C. chauvoei, C. sporogenes and, more commonly, C. perfringens (Hagemoser et al., 1980; Coloe et al., 1983; Choi et al., 2003; Peek et al., 2003; Raymundo et al., 2010). Clostridium novyi infection in horses is less frequently and usually fatal (Valberg et al., 1984; Aleman et al., 2003).

This work describes the first report involving a case of acute myonecrosis in a horse caused by C. novyi type A that most likely began after an intramuscular injection. This report emphasizes the clinical signs, pathological findings and bacteriological and phylogenetic analyses of the microorganism as the key to the definitive diagnosis.

A three-year-old horse was admitted to a veterinarian hospital due to increased volume and lameness in the left pelvic limb shown in Figure 1A. The increased in volume started in the gluteal muscle, where the horse had been given an intramuscular injection of vitamin complex.

During the hospitalization period, the treatment instituted included vertical incisions into the injured muscle to drain foul exudate and gas; these incisions were washed with hydrogen peroxide and iodine solution as shown in Figure 1B. The systemic therapy included intravenous potassium penicillin (30.000 UI/kg QID), flunixin meglumine (0.75 mg/kg TID), pentoxifylline (10 mg/kg BID), 100 mL of dimethylsulfoxide (DMSO) in 1 L of sodium chloride and 36 liters of lactated Ringers solution.

Blood samples were sent for complete blood counting and biochemistry analysis, and fluid and tissue samples were collected and sent for bacteriological examinations. The first hemogram showed no significant changes in relation to the erythrocytes; however, the level of fibrinogen was above the laboratory reference values (200400 mg/dL). The leukocyte count showed leukopenia with a left shift. The second test revealed a further increase in the fibrinogen level (1000 mg/dL) in addition to leukocytosis with neutrophilia and a regenerative shift to the left.

Microbiological growth was verified only in semisolid reinforced clostridial medium (Becton Dickinson & Co, Circle Sparks, Maryland, USA), which exhibited turbidity and gas formation. The Gram stain of a smear from the culture in reinforced clostridial medium showed pure large, gram-positive, spore-forming rods. In biochemical tests, the bacteria fermented glucose and maltose but not lactose, salicin, sorbitol or sucrose; these results were not sufficient for the accurate identification of the pathogen (Walker, 1990).

After six days of hospitalization, the prognosis of the horse was determined to be unfavorable, and the animal was euthanized. Necropsy was performed approximately 1 hour post-mortem. Tissue samples were collected and fixed in 10% buffered formalin, processed by routine histopathology techniques, cut at a thickness of 5 µm and stained with hematoxylin and eosin. At necropsy, the first description of the external examination with the location of the lesion (affected limb) was performed (Figure 1C). In some areas, subcutaneous crepitate swelling and a foul odor were present. Multifocal necrosis and the degeneration of myofibers were observed microscopically. In the interstitium, there was edema, hemorrhage and inflammatory infiltrate with a predominance of neutrophils (Figure D).

Definitive identification of the microorganism was performed by phylogenetic analysis based on a partial 16S rDNA sequence. The sequencing reaction mixtures were sequenced in triplicate using an Genetic Analyser 3100 automatic DNA sequencer ABI PRISM^® (Applied Biosystems, Foster City, CA). The SB258/09 consensus sequence of 680 bp was identified using BLASTN (NCBI website). The phylogenetic analyses based on 16S rDNA sequences showed 97% similarity between the isolated microorganism (SB 258/09) and the 16S rDNA sequence of C. novyi type A deposited in GenBank (AB045606) (Figure 2).

The diagnosis of acute myonecrosis caused by Clostridium sp. in a horse was based on clinical signs, pathological findings and the visualization of the microorganism in the exudate from the injured muscle. The etiologic diagnosis of C. novyi type A was based on genetic analysis because the morphological and biochemical results were not sufficient. In other reports identification was also confirmed by parcial or full 16S rDNA sequence analysis (McGuigan et al.; 2002; Aleman et al., 2003). The phylogenetic tree constructed to make the diagnosis presented here (Figure 2) agrees with the reported genetic relatedness among species of the genus Clostridium in other works (Collins et al., 1994; Sasaki et al., 2001).

C. novyi type A, previously called C. oedematiens, causes gas gangrene in humans and malignant edema in animals. In humans, C. novyi type A was recognized as a cause of septicemia among injecting drug users who use intramuscular injection (McGuigan et al., 2002). There have been only eight reported cases of infections of equines worldwide caused by C. novyi: five cases of acute necrotizing hepatitis, possibly caused by C. novyi type B or D (Dumaresq, 1939; Hollingsworth et al., 1978; Gay et al., 1980; Oaks et al., 1997); two cases of myonecrosis caused by a combination of C. novyi and C. septicum and by C. novyi alone, respectively (Graham, 1940; Valberg e Mc-Kinnon, 1984); and one case of a thoracic abscess from which C. novyi type A was isolated (Aleman et al., 2003). Therefore, this study is the first reported case of myonecrosis in a horse caused by C. novyi type A.

According to literature, most previously reported cases of clostridial myositis in horses developed after the intramuscular administration of nonantibiotic medication (Harwood, 1984; Brown, 1988; Peek et al., 2003; Raymundo et al., 2010). In these cases, the site of injection corresponded to site at which the clinical signs were first observed; therefore, it is assumed that the clostridial muscle infection resulted from the intramuscular injection. Although there is speculation about the manner in which clostridial spores are introduced into equine skeletal muscle and about the development of disease, some studies have raised the possibility of introducing spores via the medication or the needle (Breuhaus et al., 1983). Some researchers (Vengust et al., 2003), have shown that clostridial spores are dormant in healthy equine skeletal muscle. These researchers evaluated the hypothesis that dormant spores are capable of causing clostridial myonecrosis under the appropriate environmental conditions. Although other work (Brown, 1988) does not identify the injection site as an important factor for the development of the disease, it's suggest (Peek et al., 2003) that injection in the cervical region is a potential risk factor for clostridial myonecrosis. In this report, the application site was the gluteal muscle, an area more vascularized than the neck. However, the compound vitamin that was administered to the horse is considered to be a potential irritant, reinforcing the recommendation of some authors to avoid the intramuscular injection of nonantibiotic medications into horses whenever possible (Breuhaus et al., 1983; Rebhun et al., 1985; Peek et al., 2003).

The treatment of the horse was performed according the reported protocols for other cases of malignant edema in horses (Rebhun et al., 1985; Peek et al., 2003 Valberg e McKinnon, 1984). However, the treatment was not successful, as in other studies of infections with C. novyi (Amimoto et al., 1998); the animal was euthanized on the sixth day of hospitalization.

The observed pathological lesions were similar to those found in reports of clostridial myositis in horses and included crepitant swelling and hemorrhage, as described previously (Valberg e McKinnon, 1984); muscle necrosis, as reported anteriorlyby (Peek et al., 2003; Valberg e Mc-Kinnon, 1984); and a malodorous exudate suggestive of anaerobic infection (Aleman et al., 2003). Histopathological evaluation revealed the presence of multifocal degeneration and necrosis of the myofibers, as also described previously (Valberg e McKinnon, 1984), as well as inflammatory infiltrate with a predominance of neutrophils.

This is the first report involving C. novyi typeAasa causative agent of myonecrosis (malignant edema) in an equine, emphasizing the definitive diagnosis performed based on the phylogenetic analysis of the microorganism. Although there is a low incidence of C. novyi infection in horses, it is advisable to use alternative routes for the administration of nonantibiotic medications to avoid intramuscular injections, even in vascularized areas.

Acknowledgments

The authors thank to the Brazilian federal bureaus to facilitate scientific research (Conselho Nacional de Desenvolvimento Científico e Tecnológico/CNPq and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/CAPES) for the financial support and scholarship.

Submitted: October 24, 2012;

Approved: April 04, 2013.

All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.

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Publication Dates

• Publication in this collection
  08 Apr 2014
• Date of issue
  2014

History

• Received
  24 Oct 2012
• Accepted
  04 Apr 2013