## Print version ISSN 1517-8382On-line version ISSN 1678-4405

### Braz. J. Microbiol. vol.46 no.4 São Paulo Oct./Dec. 2015  Epub Oct 27, 2015

#### http://dx.doi.org/10.1590/S1517-838246420140188

Environmental Microbiology

Variation of Spirulina maxima biomass production in different depths of urea-used culture medium

1Korea Institute of Ocean Science & Technology, Seoul, Republic of Korea

2Department of Marine Biology, King AbdulAziz University, Jeddah, Saudi Arabia

3Department of Marine Biotechnology, Soon Chun Hyang University, Asan-si, Republic of Korea

4Department of Food Science and Engineering, Seowon University, Cheongju, Chungbuk, Republic of Korea

ABSTRACT

Fewer studies have assessed the outdoor cultivation of Spirulina maxima compared with S. platensis, although the protein content of S. maxima is higher than S. platensis. Spirulina growth medium requires an increased amount of NaHCO3, Na2CO3, and NaNO3, which increases the production cost. Therefore, the current study used a low-cost but high-efficiency biomass production medium (Medium M-19) after testing 33 different media. The medium depth of 25 cm (group A) was sub-divided into A1 (50% cover with a black curtain (PolyMax, 12 oz ultra-blackout), A2 (25% cover), and A3 (no cover). Similarly the medium depths of 30 and 35 cm were categorized as groups B (B1, B2, and B3) and C (C1, C2, and C3), respectively, and the effects of depth and surface light availability on growth and biomass production were assessed. The highest biomass production was 2.05 g L-1 in group A2, which was significantly higher (p < 0.05) than that in all other groups and sub-groups. Spirulina maxima died in B1 and C1 on the fifth day of culture. The biochemical composition of the biomass obtained from A2 cultures, including protein, carbohydrate, lipid, moisture, and ash, was 56.59%, 14.42%, 0.94%, 5.03%, and 23.02%, respectively. Therefore, S. maxima could be grown outdoors with the highest efficiency in urea-enriched medium at a 25-cm medium depth with 25% surface cover or uncovered.

Key words: Spirulina maxima; fertilizer-grade urea; medium depth; surface cover; outdoor mass culture

Introduction

Spirulina (Arthrospira) includes various species of primitive unicellular blue-green algae, most commonly S. platensis and S. maxima. Spirulina grows in shallow and highly alkaline water in tropical areas (Johnston, 1970). The Aztecs have consumed Spirulina in Mexico since the 16th century, the first tribe of hunter-collectors used it for food when they found it on margins of lakes, and even today, indigenous people consume Spirulina growing in African lakes. For the last 2-3 decades, millions of people around the world have enjoyed Spirulina as a safe food supplement. Spirulina is being commercially cultivated as a human food supplement, as an animal feed ingredient, and for pharmaceutical uses because of its ability to produce compounds such as carotene and omega 3 and 6 polyunsaturated fatty acids (Alonso and Maroto, 2000). Spirulina is also a good source of vitamin B12 with immune-promoting effects and antioxidant activity (Estrada et al., 2001; Xue et al., 2002). There are many advantages of cultivating Spirulina over traditional agriculture, such as high protein biomass, absence of processing by-product discard, suitability for arid or semi-arid areas of the world, and the fact that Spirulina can be cultured in saline water.

However, there are some disadvantages, mainly due to the mineral costs required to make suitable media for high biomass production. Spirulina is being cultured mostly in Zarrouk's or modified Zarrouk's medium or Society of Toxicology (SOT) medium, which are expensive because they require increased amounts of NaHCO3, Na2CO3, NaNO3, and trace metals. Some studies have been conducted using seawater enriched with different minerals (Lamela and Rocha, 2000; Tredici et al., 1986). Spirulina sp. have also been cultured directly in human urine in China and in effluents from pig wastewater treatment plants in Korea (Lun and Cheng, 2006; Hong and Lee, 1993). The Spirulina grown in saline water is known as S. maxima and has higher protein content than does S. platensis (Oliveira et al., 1999). There are fewer studies on S. maxima than on S. platensis, especially studies examining outdoor cultivation. In Hawaii, S. pacifica® has been developed from an S. platensis strain (Cyanotech, Kailua-Kona, HI).

In the present study, an S. maxima scale-up culture was conducted in a medium that was selected after 33 different culture media were tested using distilled water, underground water (UGW), natural seawater (NSW), and treated natural seawater (TNSW). Thereafter, the highest efficiency biomass production with the lowest cost medium was selected for scale-up mass culture. The best medium among the 33 media was made of urea, soil extract, and other nutrients from SOT medium. Urea appears to be an alternative nitrogen source that is less expensive than the conventional nitrogen source nitrate used to grow Spirulina sp. The nitrogen of urea is metabolized by cyanobacteria through enzymes such as urease and urea starch lyase (Meeks et al., 1983). The two atoms of nitrogen yielded from urea are efficiently assimilated by microalgae (Faintuch and Sato, 1992). Urea levels higher than 300-500 mg L-1 strongly inhibited the growth of Spirulina in batch culture (Torre et al., 2003). The area with dissolved urea particles becomes a zone of high pH with a higher ammonia concentration, which becomes quite toxic for several hours. At the end of the first step of the study, broken S. maxima trichomes with fewer coils (3-5 coils) and an ammonia odor were found in some culture jars containing urea-enriched medium. Costa et al. (2001) reported that ammonia or an ammonium complex compound might reduce the number of trichrome coils.

In addition, the accumulation of ammonia within the cell can occur due to low glutamine synthetase activity at low light intensities because of culture self-shadowing, and the accumulation of ammonia in cells represses urease activity (Costa et al., 2001). In addition, it is often necessary to shadow the basins to prevent the temperature or light intensity becoming too high for the application of outdoor cultures of Spirulina (Bast, 1986). Therefore, the S. maxima scale-up culture was performed at different depths and with different surface light availability using the best growth medium to identify the optimum outdoor culture conditions. Finally, the effects of depth and light availability on specific growth rate, biomass production, biochemical composition, amino acid composition, and mineral content were investigated in cultured S. maxima.

Materials and Methods

Study site and estimate of environmental factors

This study was conducted at Chuuk State, which belongs to the Federated States of Micronesia (7°29′ N, 151°50′ E, Figure 1). The Chuuk climate is warm and tropical, with air temperatures ranging from 26 to 32 °C and water temperatures of approximately 28 to 30 °C throughout the year. In the first step of the study, 28 of 33 media were made using a soil extract instead of a trace metals solution. A local soil (100 g) was diluted with 1 L of UGW and heated at 90 °C for 2 h. The soil-diluted turbid water was kept overnight at room temperature, and a 2% supernatant was added with water to create a media with the nutrients described in Table 1. The micronutrient and vitamin solution (Microvitsol) was produced by dilution of a solution with the following chemical composition: 10.00 mg L-1 H3BO3, 2.00 mg L-1 MnSO4.5H2O, 1.00, mg L-1 ZnSO4.7H2O, 1.00 mg L-1, Co(NO3)2.6H2O, 1.00, mg L-1 Na2MoO4.2H2O, 0.01 mg L-1 CuSO4.5H2O, and 0.0001 mg L-1 cyanocobalamin. Then, 1 mL of Microvitsol was added to SOT, M-2, M-4, M-6, and M-20 media, respectively. In addition, media from M-21 to M-32 were made using TNSW, which was prepared after pretreating NSW with 8.00 g L-1 of NaHCO3. All media were prepared according to the recipes described in Table 1. All of the major nutrients (NaHCO3, NaNO3, Na2CO3, and K2HPO4) were food grade, and all micronutrients were laboratory grade. Cooking salt (KOOKA iodized cooking salt, Cheetham Salt Ltd, Australia) was used instead of NaCl in M-8 to M-19 media (Table 1).

Table 1 Spirulina maxima culture media, medium component chemicals (g L-1), soil extract (SE; 2mL L-1), Micronutrients and vitamins solution (Microvitsol. 1 mL L-1) and biomass production per liter (g L-1) at the 1st step of experiment.

No. NaHCO3 Na2CO3 K2HPO4 K2SO4 MgSO4.7H2O FeSO4.7H2O Na2-EDTA NaNO3 Urea NaCl Cooking salt CaCl2. 2H2O Microvit sol SE Water Biomass
SOT 13.61 4.03 0.50 1.00 0.20 0.01 0.08 2.50 NA 1.00 NA 0.04 * NA DW 2.35 ± 0.04
M-1 13.61 4.03 0.50 1.00 0.20 0.01 0.08 2.50 NA 1.00 NA NA NA * DW 2.32 ± 0.06
M-2 13.61 4.03 0.50 1.00 0.20 0.01 0.08 2.50 NA 1.00 NA 0.04 * NA UG 2.29 ± 0.02
M-3 13.61 4.03 0.50 1.00 0.20 0.01 0.08 2.50 NA 1.00 NA NA NA * UG 2.31 ± 0.02
M-4 13.61 4.03 0.50 1.00 0.20 0.01 0.08 NA 2.50 1.00 NA 0.04 * NA DW 2.250.13
M-5 13.61 4.03 0.50 1.00 0.20 0.01 0.08 NA 2.50 1.00 NA NA NA * DW 2.27 ± 0.04
M-6 13.61 4.03 0.50 1.00 0.20 0.01 0.08 NA 2.50 1.00 NA 0.04 * NA UG 2.28 ± 0.02
M-7 13.61 4.03 0.50 1.00 0.20 0.01 0.08 NA 2.50 1.00 NA NA NA * UG 2.24 ± 0.03
M-8 5.00 3.00 0.50 1.00 0.20 0.01 0.08 2.50 NA NA 1.50 NA NA * UG 1.81 ± 0.09
M-9 5.00 2.00 0.50 1.00 0.20 0.01 0.08 2.50 NA NA 1.50 NA NA * UG 1.79 ± 0.03
M-10 5.00 1.00 0.50 1.00 0.20 0.01 0.08 2.50 NA NA 1.50 NA NA * UG 1.76 ± 0.02
M-11 5.00 3.00 0.50 1.00 0.20 0.01 0.08 NA 2.50 NA 1.50 NA NA * UG 1.95 ± 0.04
M-12 5.00 2.00 0.50 1.00 0.20 0.01 0.08 NA 2.50 NA 1.50 NA NA * UG 1.68 ± 0.07
M-13 5.00 1.00 0.50 1.00 0.20 0.01 0.08 NA 2.50 NA 1.50 NA NA * UG 1.74 ± 0.08
M-14 6.00 3.00 0.50 1.00 0.20 0.01 0.08 2.50 NA NA 1.50 NA NA * UG 1.89 ± 0.03
M-15 6.00 2.00 0.50 1.00 0.20 0.01 0.08 2.50 NA NA 1.50 Na NA * UG 1.79 ± 0.11
M-16 6.00 1.00 0.50 1.00 0.20 0.01 0.08 2.50 NA NA 1.50 NA NA * UG 1.74 ± 0.08
M-17 6.00 3.00 0.50 1.00 0.20 0.01 0.08 NA 2.50 NA 1.50 NA NA * UG 1.98 ± 0.01
M-18 6.00 2.00 0.50 1.00 0.20 0.01 0.08 NA 2.50 NA 1.50 NA NA * UG 2.01 ± 0.03
M-19 6.00 1.00 0.50 1.00 0.20 0.01 0.08 NA 2.50 NA 1.50 NA NA * UG 2.03 ± 0.02
M-20 13.61 4.03 0.50 1.00 0.20 0.01 0.08 2.50 NA NA NA NA * NA TNS X
M-21 5.00 3.00 0.50 1.00 0.20 0.01 0.08 2.50 NA NA NA NA NA * UG:TNS (1:1) 0.95 ± 0.03
M-22 5.00 2.00 0.50 1.00 0.20 0.01 0.08 2.50 NA NA NA NA NA * UG:TNS (1:1) 1.02 ± 0.07
M-23 5.00 1.00 0.50 1.00 0.20 0.01 0.08 2.50 NA NA NA NA NA * UG:TNS (1:1) 0.98 ± 0.19
M-24 5.00 3.00 0.50 1.00 0.20 0.01 0.08 NA 2.50 NA NA NA NA * UG:TNS (1:1) 0.86 ± 0.03
M-25 5.00 2.00 0.50 1.00 0.20 0.01 0.08 NA 2.50 NA NA NA NA * UG:TNS (1:1) 0.90 ± 0.08
M-26 5.00 1.00 0.50 1.00 0.20 0.01 0.08 NA 2.50 NA NA NA NA * UG:TNS (1:1) 0.89 ± 0.02
M-27 6.00 3.00 0.50 1.00 0.20 0.01 0.08 2.50 NA NA NA NA NA * UG:TNS (1:1) 0.82 ± 0.03
M-28 6.00 2.00 0.50 1.00 0.20 0.01 0.08 2.50 NA NA NA NA NA * UG:TNS (1:1) 0.89 ± 0.02
M-29 6.00 1.00 0.50 1.00 0.20 0.01 0.08 2.50 NA NA NA NA NA * UG:TNS (1:1) 0.85 ± 0.01
M-30 6.00 3.00 0.50 1.00 0.20 0.01 0.08 NA 2.50 NA NA NA NA * UG:TNS (1:1) 0.68 ± 0.02
M-31 6.00 2.00 0.50 1.00 0.20 0.01 0.08 NA 2.50 NA NA NA NA * UG:TNS (1:1) 0.75 ± 0.05
M-32 6.00 1.00 0.50 1.00 0.20 0.01 0.08 NA 2.50 NA NA NA NA * UG:TNS (1:1) 0.72 ± 0.09

*Indicates adding component, NA, Not adding; DW, Distilled water; UG, Underground water; TNS, Treated natural seawater and X, die off S. maxima.

Spirulina maxima was grown outdoors for 2 weeks in a 10-L transparent plastic jar with two replications (R1 and R2). The biomass, pH, and salinity were measured during inoculation and at the end of the study. Therefore, the second step of the study was conducted for scale-up based on the highest biomass production with the cheapest medium, which was the M-19 medium. Fiberglass-reinforced plastic tanks (95 × 46 cm) were used with different depths and surface covers. The tanks were filled with culture medium at depths of 25, 30, and 35 cm and a black curtain (PolyMax, 12 oz ultra-blackout) was used to cover the surface of the tanks at the rate of 50%, 25%, and 0% or no cover. The tanks with culture medium at a depth of 25 cm (A group) were categorized as A1 (25 cm depth with 50% cover), A2 (25 cm depth with 25% cover), and A3 (25 cm depth without cover). Culture tanks with depths of 30 and 35 cm were categorized as groups B (B1, B2, and B3) and C (C1, C2, and C3), respectively. Continuous aeration was supplied to the culture with an aeration pump at a maximum airflow of 3.32 cfm. The light intensity was measured at 0900, 1300, and 1700 with a light meter (Lux/Fc light meter 205, Tenmars Electronics, Taipei, Taiwan). The lux data were converted to μmol photons m-2 s-1 according to the formula described by Clayton (Pierre et al., 2008). Temperature, pH, and salinity were measured with a YSI probe (MPS556, YSI, Inc., Yellow Springs, OH, USA) every other day when samples were collected for estimating biomass.

Determination of specific growth and biomass production

The culture medium pH was maintained for 12 h after adjusting the initial pH to 7.5 by adding NaOH or HCl. Approximately 0.015 g L-1 (dry weight) S. maxima was inoculated and grown for 16 days. A 20-mL sample was collected from each culture tank every other day to estimate the biomass. The sample was filtered through preweighed GF/F Whatman filter paper. A preweighed filter paper that was soaked in distilled water and dried at the same time was used as a blank. The biomass filter paper was kept at 55 °C in an oven, dried and weighed, and the dry weight biomass was calculated as g L-1, which was plotted as a growth curve. The specific growth rate (μ) was defined as the increase in biomass per unit time and calculated using the following formula (Pirt, 1975).

μ(day-1)=In(X1X0)t1t0

where X0 and X1 are the biomass at the beginning (t0) and the end (t1) of a selected time interval between inoculation and maximum biomass production.

Microscopic observation

Contamination with other plankton (phytoplankton or zooplankton) and the number of coils in an S. maxima trichome were observed under an inverted light microscope (Olympus, CKX41, Tokyo, Japan). A 10-mL sample was collected at the end of the study; 4 to 6 drops of live sample were gently put on a glass slide and mixed properly, and 2 to 3 drops were covered with a cover slip. The presence of contaminants was then assessed at 400x magnification. Broken and lysed trichomes with fewer coils were found in some cultures in urea-enriched media in the first step of the study. Therefore, the number of coils in trichomes was counted in outdoor cultures to assess the variation among the culture conditions. For counting the number of coils, 1 mL of sample was placed on a Sedgwick-Rafter counter chamber, and the number of coils was counted for 50 randomly selected trichomes in each culture. Finally, the entire biomass was collected after filtering through a 20-μm mesh plankton net and was freeze dried for further study.

Biochemical composition

The biomass from the best growth condition was selected for biochemical composition analysis, and carbohydrate, protein, lipid, moisture, and ash contents were determined following the methods of the Association of Official Analytical Chemists AOAC (1995). Crude lipid was determined by Soxhlet extraction; crude protein, by the Kjeldahl method; ash, by calcinations in a furnace at 550 °C; and moisture by heating to 105 °C for 20 h.

Amino acid composition was determined following the AOAC method. Briefly, a 10-g sample was dehydrated with acetone and dried on filter paper at 60 °C in a drying oven. The dried materials were hydrolyzed in 6 N HCl for 24 h at 110 °C. After cooling, the samples were washed with 0.01 N HCl, and 2 N NaOH was added for neutralization. Supernatants were collected by centrifugation at 5,000 rpm for 30 min. The supernatants were evaporated with nitrogen gas at 60 °C and dissolved with 0.02 N HCl. The samples were filtered with a 0.45-μm filter and analyzed on a Beckman 6300 automated amino acid analyzer (Beckman Coulter, Fullerton, CA, USA). Areas of amino acid standards were used to calculate the quantity of each amino acid in the samples. Mineral content was also determined by AOAC methods using an inductively coupled spectrometer (Perkin Elmer Instruments, Shelton, CT, USA). Spirulina maxima was ashed at 550 °C for 6 h. The ash was dissolved in HNO3, filtered, and diluted with distilled water. Thereafter, the absorbance of the sample was read directly on the spectrometer.

Statistical analysis

The mean values of each treatment for temperature, pH, salinity, light intensity, specific growth, and biomass production were compared using one-way analysis of variance (ANOVA) followed by Tukey's test. A P-value of < 0.05 was considered to be significant. All statistical analyses were performed using SPSS Statistical Software, version 12.0, 12.0.1 (Edinburgh, Scotland).

Results

Dynamics of environmental factors

During the study, the sunlight intensity varied from 479 to 2,492, 429 to 3,300, and 182 to 660 μmol photon m-2s-1 at 0900, 1,300, and 1,700, respectively, with the peak at 1,300 on the sixth and eighth days of the study (Figure 2). The temperature ranged from 28.20 to 34.00 °C, with an average of 30.62 °C. The highest temperature was in tank A3, and the lowest was in C1 tank (Figures 3A). The temperature was highest in the uncovered tanks, followed by the 25% and 50% covered tanks. Salinity fluctuated from 10.72 to 13.56 psu, and the highest salinity was in tank A3 (Figure 3B). The pH varied from 7.50 to 8.49 in the A group tanks, with the highest pH in A2, followed by A1 and A3; in the B and C group tanks, the pH values were 8.76 and 8.74 in B2 and C2, respectively, although B1 and C1 had the highest pHs of 8.98 and 8.83, respectively, during culture die-off (Figures 3C).

Specific growth rate and biomass production

The maximum specific growth rate (μmax d-1) was estimated as an informative way to ascertain microbial activity, which can increase at exponential rates. Significant biological information on Spirulina mass culture can be obtained by determining growth characteristics under controlled conditions, and this information can then be applied to create a high-density mass-culture system. The μmax d-1 of S. maxima varied from 0.34 to 0.35, 0.33 to 0.36, and 0.31 to 0.35 d-1 among the A, B, and C culture groups, respectively; the highest μmax d-1 was in B2 (Figure 3D). Spirulina maxima grew well in the entire A group tanks; biomass production varied from 1.80 to 2.05 g L-1 and was highest in A2, followed by A3 and A1 (Figure 3D). In the B group tanks, the biomass production ranged from 0.55 to 1.55 g L-1, with the highest value in B3, followed by B2 (Figure 3D). In the C group tanks, the highest biomass was 1.15 g L-1 in C3 (Figure 3D). Among all nine culture conditions, A2 had the highest S. maxima biomass production.

Microscopic observation

Microscopic observations were performed to confirm the presence of other plankton in the culture and to count the numbers of trichomes coils of S. maxima. Microscopic observation of an S. maxima live sample revealed that the culture was a monostrain at the end of the study. The coil numbers in trichomes varied from 5 to 14 in the A culture group, with the maximum number in A2, and average coil numbers of 8.15 ± 1.60, 8.70 ± 2.30, and 7.75 ± 1.52 in A1, A2, and A3, respectively. Four to six coils were observed in the B group tanks, with the maximum number found in B3 (6.42 ± 0.15), followed by average values of 5.18 ± 1.1 in B2. In the C group tanks, there were 4 to 5 coils with averages of 4.29 ± 0.93 and 4.48 ± 1.53 in C2 and C3, respectively (Figure 4).

Biochemical and amino acid composition and mineral content

In the present study, the S. maxima biochemical composition was as follows: protein 56.59%, carbohydrate 14.42%, lipid 0.94%, moisture 5.03%, and ash 23.02% on a dry weight basis in the biomass of the A2 culture tank. Ten essential amino acids (threonine, valine, methionine, isoleucine, tyrosine, phenylalanine, lysine, histidine, tryptophan, and arginine) made up 5.26, 6.63, 1.37, 5.94, 4.57, 5.03, 5.26, 1.6, 0.07, and 6.86% of the S. maxima biomass, respectively, while eight nonessential amino acids (aspartic acid, serine, glutamic acid, proline, glycine, alanine, cystine, and leucine) made up 9.83, 5.03, 15.08, 3.43, 5.48, 8.23, 0.27, and 9.83%, respectively (Figure 5). Analysis of mineral content revealed that the Na, K, Fe, Mg, Ca, Mn, Zn, and P contents were 65.80, 24.26, 5.34, 3.52 2.38, 0.03, 0.01, and 13.31 mg 100 g-1, respectively (Figure 6).

Discussion

Effects of environmental factors

Spirulina maxima grew well in all tanks of the A group with different biomasses. Meteorological factors had a large influence on the growth of S. maxima, especially cloudy sky and rainfall in the outdoor mass culture in urea nitrogen medium. On the fourth and fifth days of the study, the sky was cloudy and there was heavy rain. During rainy days, the sunlight intensity decreased to the same intensity as that at 1,700 on a sunny day. The water depth in all of the tanks increased 3-5 cm due to rain, and the salinity and temperature of each tank decreased approximately 1.00 psu and 3 °C, respectively. The 35-cm deep tanks increased to 38 to 41 cm. On rainy days, a strong ammonia odor came from the 30- to 35-cm-deep tanks with 50% surface covers, and higher pH values (8.98 and 8.83 in B1 and C1) were observed in those tanks. A weak ammonia odor came from the tanks with depths greater than 30 cm with a 25% surface cover or uncovered. The S. maxima culture died in the tanks in which the ammonia odor was very strong. Urea was partly hydrolyzed to ammonia under alkaline conditions and lost by off gassing (Costa et al., 2001). Total ammonia in aqueous solution consists of two principal forms, the ammonium ion (NH4+) and un-ionized ammonia (NH3). The form depends on the pH, with ammonium (NH4+) predominating when the pH is below 8.75 and ammonia (NH3) predominating above pH 9.75 (Hydrolab, a Hatch Company). In addition, optimum activity of urease probably occurs in the pH range of 7 to 9, with little activity above a pH of 9.5 (Bast, 1986). Accumulation of ammonia within the cell can occur due to low glutamine synthetase activity at low light intensity because of self-shadowing of cultures, and accumulation of ammonia in cells represses urease activity (Costa et al., 2001). NH3 is quite toxic to seeds or plantlets because it is uncharged and lipid-soluble (AOAC, 1995), and thus traverses biological membranes more readily than the charged and hydrated NH4+ ions (Swigert, 1984; Wuhrmann and Woker, 1948). Therefore, it was assumed that the S. maxima died as a result of the ammonia derived from urea because of less light availability due to a cloudy sky, less surface area exposed to light due to the covers, or less light penetration due to greater depths. Additionally, the ammonia concentration increased due to weak mixing at increased depths during rainy days.

Specific growth rate and biomass production

Except for the Spirulina die-off tanks, the specific growth rate of S. maxima showed no significant (p < 0.05) differences among tanks of the A group. Biomass production was significantly higher (p < 0.05) in the A2 among group as well as in all culture group tanks. The μmaxd-1 and biomass production of S. maxima in this study were low in comparison with that in the SOT medium in which μmax was 0.40 d-1 and 2.70 g L-1 maximum biomass production of S. maxima (Tredici et al., 1986; Oliveira et al., 1999). Oliveira et al. (1999) reported that S. maxima growth rate and biomass production were 0.45 d-1 and 2.40 g L-1 at 35 °C, respectively, under a continuous light condition in a Paoletti medium (Downing and Merkens, 1955). The biomass production was significantly (p > 0.05) higher in B3 than C3 in tanks from groups B and C, respectively. However, the S. maxima production differed with variation in the surface cover in M-19 culture medium at the same depth. Biomass production from the A2 tanks was higher than the biomass production of S. platensis in urea using Zarrouk's medium, in which the biomass was 0.91 g L-1 in a microenvironment culture condition (Costa et al., 2001) or urea-fed batch cultured biomass (Costa et al., 2001). In most published studies, urea was used in culture media having a pH of 9.0 or above which is the alkaline condition triggering NH3 formation rather than NH4+ and decreasing or stopping urease activity, as discussed above (Costa et al., 2001; Sánchez-Luna et al., 2006; Sánchez-Luna et al., 2004; Abeliovich and Azov, 1976). In the A culture group, pH did not exceed 8.5, which might be the optimum condition in which to use urea for S. maxima growth.

Biomass production of S. maxima in M-19 medium is economically more viable, as the price of the chemicals is lower than that of Zarrouk's, SOT, or Paolett medium. Moreover, the culture system will also be effective to grow S. maxima under natural light conditions, as urea uptake by microalgae is greater in the light than in the dark (Stanca and Popovici, 1996). Therefore, a 25-cm culture medium depth with a surface cover of 50%, 25%, or 0% in regular sunlight will be a suitable condition for S. maxima growth in M-19 culture medium.

Microscopic observation

The presence of other plankton in the culture can create competition for nutrients or can be toxic if the contaminant species is toxic, like toxic cyanobacteria. In this study, the culture was contaminant-free. A culture medium prepared with 1.5% table salt using UGW protects the Spirulina from contamination with other plankton (Hodson and Thompson, 1968). Microscopic observation of a S. maxima live sample revealed that the culture was a monostrain at the end of the experiment. The number of coils in the trichomes varied among the A, B and C culture groups. There were more coils in the A group tanks than in the B and C group tanks, and the number of coils in the C tanks was almost half that in the A tanks. Short and broken S. maxima trichomes were found in tanks with a higher pH and an ammonia odor. The occurrence of ammonia in association with higher pH might have an adverse effect on the coils formation in trichomes. Similarly, Costa et al. (2001) found broken trichomes and lysed S. platensis cells in a culture medium composed of 0.01 M ammonium acid phosphate.

Biochemical and amino acid composition and mineral content

Information on the biochemical composition of Spirulina sp. grown under specific culture conditions is important for profitable commercial production, as the chemical composition may differ with variations in the physico-chemical conditions of the culture system. For example, S. maxima protein content was 70.24, 68.01, 68.67, 64.58, and 62.81% at temperatures of 20, 25, 30, 35, and 40 °C, respectively (Oliveira et al., 1999). Low protein synthesis and high carbohydrate content were found in S. maxima grown in seawater due to physiological stress (Lamela and Rocha, 2000). In our study, the protein and carbohydrate content of S. maxima also showed similar results, which might have been related to the use of cooking salt containing 50 mg g-1 potassium iodate as well as other unspecified contents rather than NaCl alone.

Protein content and amino acid composition also varied in S. platensis (Kim et al., 2007). The amino acid analysis revealed that leucine was proportionally the highest (9.83%), followed by arginine, valine, and isoleucine. Leucine is very important for health, as it is used for growth and repair of muscle tissue. It also helps to prevent muscle protein breakdown. Additionally, leucine is directly linked to the maintenance of glucose homeostasis by enhancing glucose recycling via the glucose-alanine cycle and is directly linked to the translational regulation of muscle protein synthesis through the insulin signaling cascade (Volkmann et al., 2008). Similarly, the non-amino acid S. maxima analysis showed that glutamic acid was 15.08%, followed by aspartic acid and alanine. Glutamic acid is a precursor of gamma-aminobutyric acid, which functions as an inhibitory neurotransmitter. It is one of the few nutrients that crosses the blood-brain barrier, and it is the only means by which ammonia in the brain can be detoxified. It is considered nature's “brain food” due to its association with improved mental capacity. Therefore, more leucine and glutamic acid can be obtained from S. maxima using this culture methodology. Among the S. maxima minerals, the amount of potassium was second highest in ranking. High potassium diets are beneficial for protecting against cardiovascular disease partly because of their blood-pressure-lowering effect. Low blood pressure, decreased renal tubulointerstitial injury, and suppressed renal inflammation were observed in male Sprague-Dawley rats after 8 weeks of feeding a potassium-supplemented diet (2.1% potassium) (Layman, 2003). Thus, abundant potassium can be obtained from S. maxima as a dietary source.

Conclusions

Spirulina maxima grew well at a depth of 25 cm in M-19 medium consisting of urea, soil extracts, and other chemicals. The highest biomass production was 2.05 g L-1 at a depth of 25 cm in a tank with 25% surface cover. In terms of quality, the protein content and amino acid composition were remarkable as regards to reported study elsewhere. Moreover, cost-wise calculations suggest that the preparation of 1,000 L of SOT medium would cost KRW 164205 ($147 USD), compared with KRW 61093 ($55 USD) for M-19 medium. In addition to this low cost, M-19 medium also yields high S. maxima production. In contrast, less light availability due to medium depth (> 30 cm) or meteorological conditions might have stimulated the formation of unionized ammonia (NH3), which might have been responsible for breaking, whitening trichomes and the death of S. maxima. Therefore, outdoor mass culture of S. maxima at a 25-cm depth in M-19 medium is suitable and profitable for home consumption and/or industrial-scale production, as most of the major ingredients were low-cost and locally available.

Acknowledgments

We are particularly grateful to the staff of the Department of Marine Resources in Chuuk State, Federated States of Micronesia (FSM), for allowing us to perform mass cultures in FSM. This work was supported by grants from the Korea Institute of Ocean Science and Technology (PE99213 and PE99234) and the Marine Biotechnology Program Funded by Ministry of Oceans and Fisheries, Korea (PM57770). In addition, this study was supported in part by King Abdulaziz University for writing research articles.

References

Abeliovich A, Azov Y (1976) Toxicity of ammonia to algae in sewage oxidation pounds. Appl Environ Microbiol 31:801-806. [ Links ]

Alonso D, Maroto F (2000) Plants as ‘chemical factories’ for the production of polyunsaturated fatty acids. Biotechnol Advances 18:481-497. [ Links ]

AOAC (1995) Official Methods of Analysis. 17th ed. Association of Official Analytical Chemists International, Arlington, Virginia, chapter 4, pp. 1-30. [ Links ]

Bast E (1986) Urease formation in purple sulfur bacteria (Chromatiaceae) grown on various nitrogen sources. Arch Microbiol 146:199-203. [ Links ]

Clayton RK (1970) Light and living matter, vol. 1: The physical part. McGraw-Hill Book Co., New York. [ Links ]

Costa JAV, Cozza KL, Oliveira L et al. (2001) Different nitrogen source and growth responses of Spirulina platensis in micro-environments. World J Microbiol Biotechnol 17:439-442. [ Links ]

Costa JAV, Cozza KL, Oliveira L et al. (2001) Different nitrogen source and growth responses of Spirulina platensis in microenvironments. World J Microbiol Biotechnol 17:439-442. [ Links ]

Downing KM, Merkens JC (1955) The influence of dissolved oxygen concentrations on the toxicity of un-ionised ammonia to rainbow trout (Salmo gairdnerii Richardson). Ann Appl Biol 43:243-246. [ Links ]

Estrada JEP, Bescós PB, Fresno AMV (2001) Antioxidant activity of different fractions of Spirulina platensis protein extract. II Farmaco 56:497-500. [ Links ]

Faintuch BL, Sato S (1992) Aquarone E, Emprego de Algumas Fortes Nitrogenadas na Obtengcao de Biomassa de Oscillatoria limnetica. Revista de Microbologia 23:32-36. [ Links ]

Ferraz CAM, Aquarone E, Krauter M (1985) Efeito da luz e do pH no crescimento de Spirulina maxima. Rev Microbiol 16:132-137. [ Links ]

Hodson RC, Thompson JF (1968) Metabolism of urea by Chlorella vulgaris. Plant Physiol 44:691-696. [ Links ]

Hong SS, Lee NH (1993) Growth of Spirulina platensis in effluents from wastewater treatment plant of pig farm. J Microbiol Biotechnol 3:19-23. [ Links ]

Johnston HW (1970) The biological and economic importance of algae, Part 3. Edible algae of fresh and brackish waters. Tuatara 18:19-34. [ Links ]

Kim CJ, Jung YH, Ko SR et al. (2007) Raceway cultivation of Spirulina platensis using underground water. J Microbiol Biotechnol 17:853-857. [ Links ]

Lamela T, Rocha MJF (2000) Phycocyanin production in seawater culture of Arthospira maxima. Ciencias marinas 26:607-619. [ Links ]

Layman DK (2003) The role of leucine in weight loss diets and glucose homeostasis. JN 133:261-267. [ Links ]

Lun FD, Cheng WZ (2006) Culture of Spirulina platensis in human urine for biomass production and O2 evolution. J ZUS B 7:34-37. [ Links ]

Meeks JC, Wycoff KL, Chapman JS et al. (1983) Regulation of expression of nitrate and dinitrogen assimilation by Anabaena species. App Environmental Microbiol 45:1351-1359. [ Links ]

Oliveira ACLD, Monteiro MPC, Robbs PG et al. (1999) Growth and chemical composition of Spirulina maxima and Spirulina platensis biomass at different temperatures. Aquacul Int 7:261-275. [ Links ]

Pirt SJ (1975) Principles of microbe and cell cultivation. Blackwell Scientific Publications, Oxford, 244 pp. [ Links ]

Sánchez-Luna LD, Bezerra RP, Matsudo MC et al. (2006) Influence of pH, temperature, and urea molar flowrate on Arthrospira platensis fed-batch cultivation: a kinetic and thermodynamic approach. Biotch Bioeng 96:702-711. [ Links ]

Sánchez-Luna LD, Converti A, Tonini GC et al. (2004) Continuous and pulse feedings of urea as a nitrogen source in fed-batch cultivation of Spirulina platensis. Aquacultural Eng 31:237-245. [ Links ]

Stanca D, Popovici E (1996) Urea as nitrogen source in modified Zarrouk medium. Rev Roum Biol 41:25-31. [ Links ]

Swigert JP (1984) The influence of NH3, NH4+ and pH gradients on accumulation of ammonia in blood of channel catfish Ictalurus punctatus. Doctoral dissertation, Purdu University, West Lafaette, Indiana, USA. [ Links ]

Torre P, Sassano CEN, Sato S et al. (2003) Fed-batch addition of urea for Spirulina platensis cultivation-thermodynamics and material and energy balances. Enzyme Microb Technol 33:698-707. [ Links ]

Tredici MR, Papuzzo T, Tomaselli L (1986) Ourdoor mass culture of Spirulina maxima in seawater. Appl Microbiol Biotechnol 24:47-50. [ Links ]

Volkmann H, Imianovsky U, Oliveira JLB et al. (2008) Culture of Arthrospira (Spirulina) platensis in desalinator wastewater and salinated synthetic medium: protein content and amino-acid profile. Braz J Microbiol 39:98-101. [ Links ]

Wang W, Soltero L, Zhang P et al. (2007) Renal inflammation is modulated by potassium in chronic kidney disease: possible role of Smad7. Am J Physiol Renal Physiol 293:1123-1130. [ Links ]

Wuhrmann K, Woker H (1948) Beiträge zur Toxikologie der Fische. II. Experimentelle Untersuchungen über die Ammoniak- und Blausäure vergiftung. Schweiz. Z Hydrol 11:210-244. [ Links ]

Xue C, Hu Y, Saito H et al. (2002) Molecular species composition of glycolipids from Sprirulina platensis. Food Chem 77:9-13. [ Links ]

Received: February 27, 2014; Accepted: November 13, 2014

Send correspondence to M.A. Affan. Department of Marine Biology, Faculty of Marine Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia. E-mail: affan2007@gmail.com

Associate Editor: Lara Durães Sette