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Effects of oxygen transfer coefficient on dihydroxyacetone production from crude glycerol

Abstract

The principal objective of this study was to evaluate the kinetics of dihydroxyacetone production by Gluconobacter frateurii CGMCC 5397 under different oxygen volumetric mass transfer coefficient (kLa) conditions in submerged bioreactors using biodiesel-derived crude glycerol as the carbon source. kLa is a key fermentation parameter for the production of dihydroxyacetone. Cultivations were conducted in baffled- and unbaffled-flask cultures (the kLa values were 24.32 h−1 and 52.05 h−1, respectively) and fed-batch cultures (the kLa values were held at 18.21 h−1, 46.03 h−1, and 82.14 h−1) to achieve high dihydroxyacetone concentration and productivity. The results showed that a high kLa could dramatically increase dihydroxyacetone concentrations and productivities. The baffled-flask culture (with a kLa of 52.05 h−1) favored glycerol utilization and dihydroxyacetone production, and a dihydroxyacetone concentration as high as 131.16 g/L was achieved. When the kLa was set to 82.14 h−1 in the fed-batch culture, the dihydroxyacetone concentration, productivity and yield were 175.44 g/L, 7.96 g/L/h and 0.89 g/g, respectively, all of which were significantly higher than those in previous studies and will benefit dihydroxyacetone industrial production.

Keywords
Dihydroxyacetone; kLa; Biodiesel-derived; Crude glycerol; Gluconobacter frateurii

Introduction

Dihydroxyacetone (abbreviated as DHA) is a commercially important chemical used in the cosmetic industry as a self-tanning agent.11 Brown DA. Skin pigmentation enhancers. J Photochem Photobiol B. 2001;63:148–161.,22 Levy SB. Dihydroxyacetone-containing sunless or self-tanning lotions. J Am Acad Dermatol. 1992;27(Pt 1):989-993. DHA has also been proposed to be involved in weight augmentation and fat loss, antioxidant activity, and increasing endurance capacity,33 Stanko RT, Robertson RJ, Spina RJ, Reilly JJ Jr, Greenawalt KD, Goss FL. Enhancement of arm exercise endurance capacity with dihydroxyacetone and pyruvate. J Appl Physiol (1985). 1990;68:119-124. and it offers a safe and effective therapeutic option for recalcitrant vitiligo. Additionally, DHA is an important precursor for the synthesis of various fine chemicals and pharmaceuticals and serves as a versatile building block for the organic synthesis of a variety of fine chemicals.44 Hekmat D, Bauer R, Fricke J. Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans. Bioprocess Biosyst Eng. 2003;26:109-116.

Crude glycerol, an important chemical, is abundantly available because it is the main byproduct of the conversion of vegetable oils into biodiesel. The progressive increase in biodiesel production has in turn caused a sudden increase in crude glycerol. Approximately 4.53 kg of crude glycerol are created for every 45.3 kg of biodiesel produced. The purification of crude glycerol to a chemically pure substance results in a valuable industrial chemical. However, purification is costly, and the glycerol market is already saturated. Thus, the price of crude glycerol continues to decline and directly affects biodiesel production costs.55 Ayoub M, Abdullah AZ. Critical review on the current scenario and significance of crude glycerol resulting from biodiesel industry towards more sustainable renewable energy industry. Renew Sustain Energy Rev. 2012;16:2671-2686. Therefore, it is essential to discover new applications for crude glycerol that will provide an ideal platform for chemical and pharmaceutical industries. Some value-added chemicals produced from crude glycerol have been developed, such as epichlorohydrin, DHA, 1,3-propanediol, propionic acid, polyhydroxyalkanoate,66 Pagliaro M, Ciriminna R, Kimura H, Rossi M, Della Pina C. From glycerol to value-added products. Angew Chem Int Ed Engl. 2007;46:4434-4440. erythritol,77 Rymowicz W,Rywinska A, Marcinkiewicz M. High-yield production of erythritol from raw glycerol in fed-batch cultures of Yarrowia lipolytica. Biotechnol Lett. 2009;31:377-380. citric acid,88 Rywinska A, Rymowicz WZarowska B, Wojtatowicz M. Biosynthesis of citric acid from glycerol by acetate mutants of Yarrowia lipolytica in fed-batch fermentation. Food Technol Biotechnol. 2009;47:1-6. hydrogen,99 Sabourin-Provost G, Hallenbeck PC. High yield conversion of a crude glycerol fraction from biodiesel production to hydrogen by photofermentation. Bioresour Technol. 2009;100:3513-3517. mannitol,1010 Khan A, Bhide A, Gadre R. Mannitol production from glycerol by resting cells of Candida magnoliae. Bioresour Technol. 2009;100:4911-4913. lipids,1111 Liang Y,Sarkany N, Cui YBlackburn JW. Batch stage study of lipid production from crude glycerol derived from yellow grease or animal fats through microalgal fermentation. Bioresour Technol. 2010;101:6745-6750. mixed acids,1212 Forrest AK, Sierra R, Holtzapple MT. Effect of biodiesel glycerol type and fermentor configuration on mixed-acid fermentations. Bioresour Technol. 2010;101:9185-9189. and eicosapentaenoic acid.1313 Athalye SK, Garcia RA, Wen Z. Use of biodiesel-derived crude glycerol for producing eicosapentaenoic acid (EPA) by the fungus Pythium irregulare. J Agric Food Chem. 2009;57:2739-2744.

Nomenclature Xmax maximum cell concentration (g/L) Pmax maximum production (DHA) concentration (g/L) T fermentation time to reach the maximum production of DHA (h) µ specific growth rate (h−1) µmax maximum specific growth rate (h−1) Qp DHA productivity (g/L/h)

Since Bertrand first observed the production of DHA from glycerol by bacteria in 1898, various microorganisms used for DHA production have been reported.1414 Mishra R, Jain SR, Kumar A. Microbial production of dihydroxyacetone. Biotechnol Adv. 2008;26:293-303.Gluconobacter strains produce DHA via the incomplete oxidation of glycerol with membrane-bound glycerol dehydrogenases (GDHs)1515 Deppenmeier U, Hoffmeister M, Prust C. Biochemistry and biotechnological applications of Gluconobacter strains. Appl Microbiol Biotechnol. 2002;60:233-242. and are the most extensively used microorganisms for DHA production.1616 Hu ZC, Zheng YG, Shen YC. Dissolved-oxygen-stat fed-batch fermentation of 1,3-dihydroxyacetone from glycerol by Gluconobacter oxydans ZJB09112. Biotechnol Bioprocess Eng. 2010;15:651-656.,1717 Wethmar M, Deckwer WD. Semisynthetic culture medium for growth and dihydroxyacetone production by Gluconobacter oxydans. Biotechnol Tech. 1999;13:283-287. During cell growth, oxygen demand is extremely high because Gluconobacter strains prefer a respiratory, rather than a fermentative, mode of growth.1818 Prust C, Hoffmeister M, Liesegang H, et al. Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans. Nat Biotechnol. 2005;23:195-200. In addition, GDH also requires oxygen to accomplish the oxidative conversion of glycerol to DHA.1919 Claret C, Salmon J, Romieu C, Bories A. Physiology of Gluconobacter oxydans during dihydroxyacetone production from glycerol. Appl Microbiol Biotechnol. 1994;41:359-365. Thus, the supply of oxygen to Gluconobacter strains is one of the most crucial factors for DHA production in an industrial process.

Various fermentation strategies have been previously reported to enhance oxygen supply during the DHA production process. Hydrogen peroxide and p-benzoquinone have been added to media as an oxygen source and an electron acceptor.2020 Holst O, Lundback H, Mattiasson B. Hydrogen peroxide as an oxygen source for immobilized Gluconobacter oxydans converting glycerol to dihydroxyacetone. Appl Microbiol Biotechnol. 1985;22:383-388. Oxygen carriers such as hemoglobin, perfluorochemicals, and silicone oils have also been used to enhance oxygen supply in fermentation media.2121 Leung R, Poncelet D, Neufeld RJ. Enhancement of oxygen transfer rate using microencapsulated silicone oils as oxygen carriers. J Chem Technol Biotechnol. 1997;68:37-46. Moreover, some conventional techniques to improve the rate of oxygen transfer from the gas phase have been investigated, including increasing the agitation or aeration rate, raising the partial pressure of oxygen in the gas phase or modifying the bioreactor design.44 Hekmat D, Bauer R, Fricke J. Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans. Bioprocess Biosyst Eng. 2003;26:109-116.

The effects of different oxygen supply conditions can be measured by the oxygen volumetric mass transfer coefficient (kLa). The oxygen volumetric mass transfer coefficient is generally considered to be a critical parameter in aerobic cultures of microorganisms due to its poor solubility and the need for a constant supply. The selection, design, and scale-up of biochemical reactors and the accurate estimation of mass transfer rates for different scales and different operational conditions are of critical importance in bioprocesses.2222 Galaction AI, Cascaval D, Oniscu C, Turnea M. Prediction of oxygen mass transfer coefficients in stirred bioreactors for bacteria, yeasts and fungus broths. Biochem Eng J. 2004;20:85-94. As with any other transport phenomena occurring between different phases, the oxygen transfer rate is profoundly affected by hydrodynamic conditions in the bioreactor, which affect the mass transfer coefficient and the interfacial area. Therefore, variations in the configurations of bioreactors and the physicochemical properties of the culture medium, including density, diffusivity, rheological properties, and surface tension, can attenuate the oxygen transfer capacity, resulting in oxygen depletion.2323 Garcia-Ochoa F, Gomez E. Prediction of gas-liquid mass transfer coefficient in sparged stirred tank bioreactors. Biotechnol Bioeng. 2005;92:761-772.

Previously, a newly isolated strain, Gluconobacter frateurii CGMCC 5397, was shown to utilize inexpensive biodiesel-derived crude glycerol to produce DHA.2424 Liu YP, Sun Y,Tan C, et al. Efficient production of dihydroxyacetone from biodiesel-derived crude glycerol by newly isolated Gluconobacter frateurii. Bioresour Technol. 2013;142:384-389. However, in our previous study, DHA concentration and productivity were not satisfactory. In this study, the effect of the oxygen transfer coefficient on DHA production from biodiesel-derived crude glycerol by Gluconobacter frateurii was investigated to enhance DHA concentration and productivity. Both baffled- and unbaffled-flask cultures were used in the fermentation process, and fed-batch cultures with three different kLa values were also compared.

Materials and methods

Microorganism

The strain Gluconobacter frateurii CGMCC 5397, isolated from rotting fruits using a crude glycerol culture in our laboratory, was employed in this work, stored at 4 °C on GY agar slants, and transferred monthly. The medium (GY) consisted of 25 g/L glycerol, 5 g/L yeast extract, and 20 g/L agar.

Crude glycerol

Crude glycerol used in this work was obtained from a palm oil-based biodiesel plant in Malaysia operated by Vance Bioenergy [composition: 80.5% (w/w) glycerol, 10.1% (w/w) water, 5.2% (w/w) sodium salts, 0.4% (w/w) potassium salts, 0.3% (w/w) other salts, 0.5% (w/w) methanol, and 2% (w/w) other organics (esters, free fatty acids, soaps, etc.)]. Crude glycerol was used as a carbon source in the culture medium without purification. The purity of the crude glycerol (80.5%, w/w) used in each experiment was taken into consideration when making the appropriate calculations; thus, the initial concentrations of glycerol quoted refer to pure glycerol.

Shake flask cultures

A crude glycerol concentration gradient (50 g/L, 75 g/L, 100 g/L, 125 g/L and 150 g/L) was used in the fermentation media of both the baffled and unbaffled flasks to compare the effects of kLa on glycerol oxidation, cell growth and DHA production. The other fermentation medium components included 24 g/L corn steep liquor and 3 g/L CaCO3. The seed medium contained 15 g/L crude glycerol, 15 g/L yeast extract, and 3 g/L KH2PO4. The pH of the medium was adjusted to 6.0 with 2 M NaOH, and the medium was heat sterilized (20 min at 121 °C). The seed medium was inoculated with a 2 mL stock culture stored at −80 °C and incubated at 30 °C shaking at 200 rpm for 16 h. For the fermentation experiments, the medium was inoculated with 5% of the seed culture and incubated shaking at 200 rpm at 30 °C for 48 h. The cells were grown in 250-mL flasks with a working volume of 50 mL.

During the cultivation process, 1-mL samples were taken from each flask every 8 h to determine the residual glycerol and biomass concentrations. At the end of the fermentation process, 1-ml samples were taken from the flask every 2 h, and the residual glycerol concentration was determined. When glycerol was consumed to less than 5 g/L in the flasks (except for some unbaffled-flask cultures that were ended earlier because the glycerol could not be exhausted to less than 5 g/L due to culture limitations), the DHA concentration and yield were analyzed.

Fed-batch cultures

Fed-batch cultures were performed in a 5-L bioreactor (Baoxing Bio-Engineering Equipment Co., Ltd, China) with 3 L of medium or in a 30-L NBS bioreactor (BioFlo 4500, New Brunswick Scientific, Edison, NJ, USA) with 20 L of medium. The fermentation was performed in the same medium as that of the flask cultures except that the initial crude glycerol concentration was 60 g/L. The inoculum volume was 5% (v/v) of the initial fermentation medium. A sterile glycerol solution (60%, v/v) was fed with a peristaltic pump. A continuous feedback control strategy was employed in the fed-batch cultures, in which the feeding rate was adjusted every 2 h to maintain the residual crude glycerol concentration within 5–25 g/L.

During the fermentation process, the pH was automatically maintained at 6.0 with NaOH (2 M). The cultivation temperature was kept at 30 °C. DO was monitored using a polarographic electrode and was expressed as a percentage of O2 saturation. The foam was controlled automatically by the addition of polyethylene glycol as an antifoaming agent. Samples were withdrawn from the bioreactor every four hours to measure DHA, glycerol and biomass contents. A 50-mL sample was taken every 8 h to determine dry cell weight.

The agitation and air-flow rates were set at as follows to achieve different kLa values: 18.21 h−1 (300 rpm and 0.12 m3/h, respectively, in the 5-L bioreactor), 46.03 h−1 (450 rpm and 0.21 m3/h, respectively, in the 5-L bioreactor), and 82.14 h−1 (500 rpm and 2 m3/h, respectively, in the 30-L bioreactor).

Analytical methods

After the desired incubation period, the culture was diluted with HCL (0.2 M), and the cell concentration (OD560) was determined by measuring the absorbance at 560 nm using a spectrophotometer (U-752). The samples were centrifuged at 8000 g for 5 min, washed twice, and dried at 80 °C to a constant weight. The following relation resulted after calibration: dry cell weight [DCW, g/L] = 0.3 × OD560.

DHA and glycerol were analyzed using high-performance liquid chromatography (HPLC). Fermentation samples were centrifuged (8000 × g, 10 min) at 4 °C, and the concentrations of DHA and glycerol in the supernatants were quantified using an HPLC system (Waters, Milford, MA) equipped with a refractive index detector, a UV detector (at 270 nm), and an Aminex HPX-87H column (300 mm × 7.8 mm, 9 µm; Bio-Rad Chemical Division, Richmond, CA). The mobile phase was an 8-mmol/L H2SO4 solution at a flow rate of 0.5 mL/min, and the column was operated at 55 °C.

DHA yield was defined as the amount of DHA produced from each gram of glycerol and was normalized in accordance with the dilution factor of the base or the crude glycerol solution.

Measurement of the oxygen transfer coefficient (kLa), µmax, and Qp,

The sulfite method (Na2SO3 method) was used for estimating the oxygen transfer coefficient, kLa, in the bioreactors.2525 Puskeiler R, Weuster-Botz D. Combined sulfite method for the measurement of the oxygen transfer coefficient k(L)a in bio-reactors. J Biotechnol. 2005;120:430-438.

The maximum specific growth rate ( µmax) was determined by performing exponential regressions on the data points from the exponential phase. To avoid the decrease of volume in the flask cultures, the µmax determination experiments were conducted independently, and 1-mL samples were taken from each flask once an hour during the exponential growth phase.

DHA productivity (Qp) was expressed as DHA concentration per hour.

All the above measurements, including dry cell weight (DCW), glycerol concentration, DHA concentration and kLa, were conducted in triplicate, and the average values with standard deviations were recorded.

Results

Effects of kLa on shake-flask cultures

Many researchers have demonstrated that baffles in flasks enhance agitation and increase the available surface area for oxygen transfer at the air–liquid interface2626 Gupta A, Rao G. A study of oxygen transfer in shake flasks using a non-invasive oxygen sensor. Biotechnol Bioeng. 2003;84:351-358.; thus, baffled flasks have a higher kLa than unbaffled flasks under identical shaking speeds.

In this work, the kLa values in the unbaffled and baffled flasks were 24.32 h−1 and 52.05 h−1, respectively. The difference in these kLa values led to differing performances in crude glycerol oxidation, biomass production and DHA accumulation (Tables 1 and 2). In the unbaffled-flask cultures, the final DHA concentration increased significantly as the initial glycerol concentration increased from 50 g/L to 100 g/L. However, the DHA concentration increased little when the glycerol concentration was over 100 g/L, as was previously reported19,24,27; the initially high glycerol concentration exerts an inhibitory effect on DHA production. In our previous work, we have shown that some poisonous coproducts (methanol, chloride salts, metal ion, etc.) in the biodiesel-derived crude glycerol affect DHA production. In the baffled-flask cultures, however, varying initial concentrations of glycerol were all efficiently exhausted to below 5 g/L after a relatively short fermentation time, and the incubation times of the unbaffled-flask cultures were 2- to 3-fold longer than those of the baffled-flask cultures with identical glycerol concentrations. As the initial glycerol concentrations increased, the biomass exhibited a continuous decline, and the highest biomass levels (unbaffled-flask cultures: 1.81 ± 0.17 g/L; baffled-flask cultures: 1.87 ± 0.10 g/L) were obtained when the glycerol concentration was 50 g/L.

In the baffled-flask cultures, the yield increased almost linearly from 0.76 g/g to 0.87 g/g as the initial glycerol concentrations increased from 50 g/L to 150 g/L. In contrast, in the unbaffled-flask cultures, the yield increased when the glycerol concentration was lower than 100 g/L and then decreased with increasing glycerol concentration; compared with the baffled-flask cultures, the yield was lower under the same conditions. The DHA productivity of the baffled-flask cultures was approximately 3-fold higher than that of the unbaffled-flask cultures with identical glycerol concentrations, which indicates that oxygen was one of the most crucial factors for DHA production, as expected.18,19

For the baffled flask with an initial glycerol concentration of 150 g/L, the DHA concentration achieved was as high as 131.16 g/L, which was slightly higher than that achieved by Liu et al.2424 Liu YP, Sun Y,Tan C, et al. Efficient production of dihydroxyacetone from biodiesel-derived crude glycerol by newly isolated Gluconobacter frateurii. Bioresour Technol. 2013;142:384-389. (125.8 g/L in a fed-batch fermentation with a 5-L bioreactor).

Therefore, observing that a high kLa (52.05 h−1) in the baffled-flask culture favored glycerol utilization and DHA production by Gluconobacter frateurii CGMCC 5397, we were further inspired to investigate the kLa parameter in larger-scale bioreactors.

Effects of kLa on fed-batch cultures

Gluconobacter frateurii CGMCC 5397 fermentations were performed at larger scales.

The above results show that substrate inhibition clearly limits DHA production in flask cultures. Fed-batch fermentations with biodiesel-derived crude glycerol at different kLa values were investigated to enhance the production of DHA. Moreover, as reported in our previous work, cell growth and DHA production were not influenced by biodiesel-derived crude glycerol versus pure glycerol fermentation in fed-batch cultures. Therefore, the initial glycerol concentration in the fed-batch cultures was set at 60 g/L to prevent inhibition from occurring in bioreactors after the evaluation of various kLa values. The glycerol concentration was controlled at 5–25 g/L.

The time courses for cell growth, glycerol consumption, and DHA production by Gluconobacter frateurii CGMCC 5397 at three kLa values (18.21 h−1, 46.03 h−1, and 82.14 h−1) are depicted in Fig. 1. In the culture with the lowest kLa, 18.21 h−1 (Fig. 1A), the rate of glycerol consumption was so low that the fermentation was sustained for 100 h, presumably due to the weak metabolic activity of the cells enduring limited oxygen transfer. Consequently, the production of DHA was only 68.27 g/L at the end of the fermentation. More glycerol was used as a carbon source for cell growth than for DHA biosynthesis, resulting in low DHA production. At the end of the fermentation, DHA production declined, which indicates that DHA can be used for supplying energy, and the DHA concentration decreased when glycerol in the medium was exhausted.2828 Batzing BL, Claus GW. Biphasic growth of Acetobacter suboxydans on a glycerol-limiting medium. J Bacteriol. 1971;108:592-595.

Fig. 1
Time course of DHA production by Gluconobacter frateurii CGMCC 5397 at different kLa values: (A) 18.21 h−1, (B) 46.03 h−1 and (C) 82.14 h−1. Glycerol, filled squares; DHA, filled circles; DCW, unfilled triangles. The experiments consisted of fed-batch cultures fed crude glycerol in triplicate. The data points are the means of the triplicate measurements.

When the kLa was increased from 18.21 h−1 to 46.03 h−1 (Fig. 1B), DHA production improved approximately two-fold, which indicates that substrate assimilation was obviously active. The glycerol feed started at 16 h, which was earlier than in the low-kLa fermentation (36 h), and was 8.13 g/L. At 70 h, 121.69-g/L DHA production was achieved.

When the kLa was elevated to 82.14 h−1 (Fig. 1C), the final DHA concentration, productivity and yield reached 175.44 g/L, 7.96 g/L/h and 0.89 g/g, respectively, the values of which were significantly greater than those above, and no inhibitory effects were observed. The glycerol feed started at 10 h, and cultivation required only 40 h. The DHA concentrations reported in this study are higher than those reported previously within the same fermentation timeframes.1818 Prust C, Hoffmeister M, Liesegang H, et al. Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans. Nat Biotechnol. 2005;23:195-200.,1919 Claret C, Salmon J, Romieu C, Bories A. Physiology of Gluconobacter oxydans during dihydroxyacetone production from glycerol. Appl Microbiol Biotechnol. 1994;41:359-365.

Discussion

Kinetic analysis of DHA fermentation at different kLa values

The above results (Tables 1 and 2) show that glycerol assimilation and cell growth were inhibited by high concentrations of glycerol, and the µmax decreased with increasing glycerol concentrations. However, compared with the unbaffled-flask cultures, the µmax from the baffled-flask cultures increased significantly, and substrate inhibition was substantially relieved under the same fermentation conditions. The baffles in the flasks enhance agitation and increase the available surface area for oxygen transfer at the air–liquid interface; thus, the baffled flasks have a higher kLa than unbaffled flasks at identical shaking speeds. In the baffled flasks (Table 2), the substrate was exhausted faster than in the unbaffled flasks. Therefore, the results obtained in this study demonstrate that the reason that substrate inhibition appeared in the unbaffled-flask cultures was not only due to high concentrations of glycerol itself but was much more likely caused by the limited oxygen availability that resulted from a low kLa (24.32 h−1).

Table 1
The effects of different initial carbon concentrations on cell growth and DHA production in unbaffled flasks (kLa, 24.32 h−1).
Table 2
The effects of different initial carbon concentrations on cell growth and DHA production in baffled flasks (kLa, 52.05 h−1).

To analyze the kinetic characteristics of the fed-batch fermentation at different kLa values, two kinetic parameters, µ and Qp (DHA productivity), were calculated based on the data in Fig. 1. As shown in Fig. 2, compared with kLa values of 18.21 h−1 and 46.03 h−1, µ was higher with a kLa of 82.14 h−1 early in the DHA fermentation (before 12 h) and then fell rapidly later in the fermentation (after 12 h). The maximum amounts of biomass produced at the different fermentation kLa values (18.21 h−1, 46.03 h−1 and 82.14 h−1) were 2.28 g/L, 2.44 g/L, and 2.23 g/L, respectively, which indicates that the cells grew best at a kLa value of 46.03 h−1. When the fermentation kLa was less than 18.21 h−1, the dissolved oxygen was so low that it limited cell growth. However, when the kLa exceeded 82.14 h−1, the agitation rate was too high, and the cells were exposed to high shear rates, which caused cell deformation and damage. In addition, high oxygen levels might cause cellular damage as well.2121 Leung R, Poncelet D, Neufeld RJ. Enhancement of oxygen transfer rate using microencapsulated silicone oils as oxygen carriers. J Chem Technol Biotechnol. 1997;68:37-46.

Fig. 2
Comparison of the kinetic parameters in DHA fed-batch fermentation by Gluconobacter frateurii CGMCC 5397 with a kLa of 18.21 h−1 (short dashed line), 46.03 h−1 (dash dotted line) or 82.14 h−1 (solid line). Specific cell growth rate, µ; DHA productivity, Qp.

A comparison of the Qp values in all the experiments indicates that the Qp increased with increasing kLa. However, late in the high-kLa fermentation (after 40 h), the synthesis of DHA ceased. Previous studies have shown that high DHA concentrations can inhibit DHA production.2929 Bauer R, Katsikis N, Varga S, Hekmat D. Study of the inhibitory effect of the product dihydroxyacetone on Gluconobacter oxydans in a semi-continuous two-stage repeated-fed-batch process. Bioprocess Biosyst Eng. 2005;28:37-43. When the DHA concentration in the culture medium reached 108 g/L, glycerol conversion ceased and no more DHA was produced. It was also reported that high DHA concentrations might cause irreversible cell damage.29,30 However, in our work, no inhibitory effects were observed when the DHA concentration was lower than 150 g/L, and the maximum DHA concentration reached was 175.44 g/L.

A consistently high kLa during DHA production led to high dissolved oxygen (DO) concentrations (Fig. 3). For cultures with kLa values of 18.21 and 46.03 h−1, the DO decreased quickly, reaching zero at 4 and 8 h of cultivation, respectively. The dissolved oxygen was unable to meet cell depletion, and DHA synthesis was insufficient. When the kLa was elevated to 82.14 h−1, the DO decreased in the first 12 h and stabilized at approximately 40%, which was suitable for DHA production.3131 Hu ZC, Liu ZQ, Zheng YG, Shen YC. Production of dihydroxyacetone from glycerol by Gluconobacter oxydans ZJB09112. J Microbiol Biotechnol. 2010;20:340-345. The effects of DO concentration on DHA production by Gluconobacter strains and the application of a DO control strategy to DHA production have been studied by several research groups.2222 Galaction AI, Cascaval D, Oniscu C, Turnea M. Prediction of oxygen mass transfer coefficients in stirred bioreactors for bacteria, yeasts and fungus broths. Biochem Eng J. 2004;20:85-94.,2323 Garcia-Ochoa F, Gomez E. Prediction of gas-liquid mass transfer coefficient in sparged stirred tank bioreactors. Biotechnol Bioeng. 2005;92:761-772.,3131 Hu ZC, Liu ZQ, Zheng YG, Shen YC. Production of dihydroxyacetone from glycerol by Gluconobacter oxydans ZJB09112. J Microbiol Biotechnol. 2010;20:340-345. Flickinger and Perlman found that DO concentration played a vital role in the microbial production of DHA from glycerol. However, DO control is impractical in industrialization because DO varies with agitation speed, cell concentration, carbon source feeding rate and concentration, bioreactor size, etc. Alternatively, kLa control can be considered a promising strategy for improving the supply and transfer of oxygen in vivo because it has been correlated with the combination of stirrer speed, superficial gas velocity and liquid effective viscosity, which are measurable scale-up parameters.3232 Qu L, Ji XJ, Ren LJ, et al. Enhancement of docosahexaenoic acid production by Schizochytrium sp. using a two-stage oxygen supply control strategy based on oxygen transfer coefficient. Lett Appl Microbiol. 2011;52:22-27.

Fig. 3
Profiles of dissolved oxygen concentrations in fed-batch DHA fermentations with crude glycerol by Gluconobacter frateurii CGMCC 5397 at different kLa values: 18.21 h−1 (short dashed line), 46.03 h−1 (dash dotted line) and 82.14 h−1 (solid line).

The biosynthesis of DHA in Gluconobacter cells is complicated, and there are three possible pathways for DHA production. The enzyme responsible for the oxidative reaction is a membrane-bound, pyrroloquinoline quinine (PQQ)-dependent glycerol dehydrogenase (GDH) that makes use of oxygen as the final electron acceptor without NADH mediation.4,19 In this study, it was found that consistently high kLa values were optimal for DHA production (Table 3). When the fermentation kLa was less than 18.21 h−1, the DHA yield was lower than reported in other experiments, which indicates that a greater proportion of carbon resources (glycerol) was used for cell growth and metabolic demand. In our experiments, glycerol was used not only as a substrate but also as carbon source for cellular processes and metabolic demand. When the fermentation kLa was less than 82.14 h−1, the proportion of carbon resources (glycerol) used for DHA biosynthesis increased, resulting in a higher DHA yield at the end of the fermentation.

Table 3
Effects ofkLa values on DHA fermentation.

In conclusion, the results from both the batch and fed-batch cultures at different scales clearly indicate that high kLa values (52.05 h−1 for baffled flasks and 82.14 h−1 for a 30-L bioreactor) effectively improved DHA concentration, productivity and yield. As kLa is a scale-up parameter and crude glycerol is a relatively inexpensive raw material, the method developed in this paper can be scaled up to industrial processes and applied to other industrial biotechnological processes to achieve both high product concentrations and high productivities.

  • Associate Editor: Jorge Gonzalo Farias Avendano

Acknowledgements

This research was supported by grants from the Key Project of Science and Technology of Henan Province (No. 132102210387), the Key Project of the Henan Educational Committee (No. 14B180016), and the Foundation for University Young Backbone Teachers of Henan Province (No. 2014GGJS-157).

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Publication Dates

  • Publication in this collection
    Jan-Mar 2016

History

  • Received
    3 Dec 2014
  • Accepted
    9 July 2015
Sociedade Brasileira de Microbiologia USP - ICB III - Dep. de Microbiologia, Sociedade Brasileira de Microbiologia, Av. Prof. Lineu Prestes, 2415, Cidade Universitária, 05508-900 São Paulo, SP - Brasil, Ramal USP 7979, Tel. / Fax: (55 11) 3813-9647 ou 3037-7095 - São Paulo - SP - Brazil
E-mail: bjm@sbmicrobiologia.org.br