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Brazilian Journal of Microbiology

Print version ISSN 1517-8382On-line version ISSN 1678-4405

Braz. J. Microbiol. vol.49 no.1 São Paulo Jan./Mar. 2018

http://dx.doi.org/10.1016/j.bjm.2017.04.002 

Environmental Microbiology

Research Paper

Mangrove endophyte promotes reforestation tree (Acacia polyphylla) growth

Renata Assis Castroa  b 

Manuella Nóbrega Douradoc  * 

Jaqueline Raquel de Almeidaa 

Paulo Teixeira Lacavad 

André Navee 

Itamar Soares de Melof 

João Lucio de Azevedoa  b 

Maria Carolina Quecinea 

aUniversity of São Paulo, Escola Superior de Agricultura “Luiz de Queiroz”, Department of Genetics, Piracicaba, SP, Brazil

bUniversity of São Paulo, Center for Nuclear Energy in Agriculture (CENA), Piracicaba, SP, Brazil

cUniversity of São Paulo, Biomedical Science Institute, Department of Microbiology, São Paulo, SP, Brazil

dFederal University of São Carlos (UFSCar), Center for Biological and Health Sciences, Department of Morphology and Pathology, São Carlos, SP, Brazil

eBIOFLORA Comercial LTDA, Piracicaba, SP, Brazil

fEMBRAPA Environment, Laboratory of Environmental Microbiology, Jaguariuna, SP, Brazil

ABSTRACT

Mangroves are ecosystems located in the transition zone between land and sea that serve as a potential source of biotechnological resources. Brazil's extensive coast contains one of the largest mangrove forests in the world (encompassing an area of 25,000 km2 along all the coast). Endophytic bacteria were isolated from the following three plant species: Rhizophora mangle, Laguncularia racemosa and Avicennia nitida. A large number of these isolates, 115 in total, were evaluated for their ability to fix nitrogen and solubilize phosphorous. Bacteria that tested positive for both of these tests were examined further to determine their level of indole acetic acid production. Two strains with high indole acetic acid production were selected for use as inoculants for reforestation trees, and then the growth of the plants was evaluated under field conditions. The bacterium Pseudomonas fluorescens (strain MCR1.10) had a low phosphorus solubilization index, while this index was higher in the other strain used, Enterobacter sp. (strain MCR1.48). We used the reforestation tree Acacia polyphylla. The results indicate that inoculation with the MCR1.48 endophyte increases Acacia polyphylla shoot dry mass, demonstrating that this strain effectively promotes the plant's growth and fitness, which can be used in the seedling production of this tree. Therefore, we successfully screened the biotechnological potential of endophyte isolates from mangrove, with a focus on plant growth promotion, and selected a strain able to provide limited nutrients and hormones for in plant growth.

Keywords: Endophytic bacteria; IAA; Phosphorus and plant growth promoting bacteria (PGPB)

Introduction

Mangroves are an important ecosystem in tropical biomes that occupy several million hectares of coastal area worldwide.1 Brazil possesses one of the largest mangrove forests, covering an area of 25.000 km2 all along the coast. This ecosystem is located in the transition zone between land and sea2 and is characterized by periodic flooding, resulting in a unique environment with few plant species. Brazilian mangroves primarily comprise the following three tree species: Rhizophora mangle, Laguncularia racemosa and Avicennia sp.3 Furthermore, the mangroves harbor a diverse group of microorganisms.4,5 Several studies have examined the microbial community of mangroves by using metagenomic approaches to access the microorganisms involved in carbon,6 nitrogen7 and sulfer8 metabolism. Despite the high microbial diversity of mangroves, estimates suggest that less than 5% of species in this environment have been described.5

Moreover, the high diversity of culturable bacteria3 and culturable endophytic fungi9 within the Brazilian mangroves has not yet been explored. Few studies focus on the biotechnological potential of culturable mangrove isolates. Castro10 screened for enzymes for use in industrial processes, such as amylase, esterase, lipase, protease and endoglucanase. This large amount of microbial diversity can be exploited to improve crop science since the microorganisms produce phytohormones, such as indole acetic acid (IAA), enzymes, and antimicrobial molecules, and solubilize phosphate in the host plant.11,12 In addition, these organisms can fix nitrogen13 and increase drought resistance.14 More recently, the high tolerance of these microorganism to heavy metal was described15,16 in addition to characteristics that are important to the promotion of plant growth.

Bacteria that exhibit these features can be used to promote the growth of different plant species such as corn, soybeans, and sugarcane as well as arboreal species.17 These beneficial characteristics of the plant-microbe interactions can be used in other plants. Cross-colonization is common in nature in which the same bacterium can colonize different host plants. One example of cross-colonization is Pantoea agglomerans isolated from Eucalyptus grandis, which is able to colonize and promote plant growth in sugarcane.12 However, there are few studies evaluating the effects of bacterial inoculation in trees.18 The tree species Acacia polyphylla, of the Leguminosae family, commonly known as “monjoleiro” in Brazil, is widely used for the reforestation of degraded areas due to its ability to fix nitrogen19 and improve degraded soils, thus decreasing costs and benefitting the environment.20 Therefore, the aim of this study is to identify and analyze the biotechnological potential of endophytic bacteria isolated from a Brazilian mangrove environment and select strains able to promote the growth of A. polyphylla.

Materials and methods

Endophyte isolation sites

Mangrove forest samples were previously collected from São Paulo state, Brazil, as described by Castro.10 The following three locations were assessed: (A) the Bertioga location, which was contaminated by oil spills; (B) the uncontaminated Bertioga location, with anthropogenic impacts; and (C) the uncontaminated Cananéia location, with low anthropogenic impacts. The following three mangrove species were assessed: (1) R. mangle, (2) L. racemosa and (3) Avicennia sp. The oil spill in Bertioga occurred approximately 20 years ago, and the anthropogenic impacts (domestic and industrial sewer) are still occurring in Bertioga at both locations sampled.6,10

From the whole mangrove bacterial collection, we randomly selected 115 isolates that were endophytically isolated from the branches of mangrove plants belonging to the culture collection of the Laboratory of Bacterial Genetics Microorganism, School of Agriculture Luiz de Queiroz (Esalq).3,10

Selection of endophytes: nitrogen fixation

We started our screening by evaluating the ability of the randomly selected 115 strains to fix atmospheric nitrogen. Qualitative assays were performed using the process of Liba.21 The strains were inoculated in tubes containing 10 mL semi-solid NFb medium (5 g L-1 malic acid, 0.5 g L-1 K2HPO4, 0.2 g L-1 MgSO4.7H2O, 0.1 g L-1 NaCl, 0.01 g L-1 CaCl2·2H2O, and 4 mL 1.64% Fe-EDTA), 2 mL 0.5% bromothymol blue, 2 mL micronutrients (0.2 g L-1 Na2MoO4·2H2O, 0.235 g L-1 MnSO4·H2O, 0.28 g L-1 H3BO3, and 0.008 g L-1 CuSO4·5H2O), and 1.75 g L-1 agar. Bacterial growth was evaluated after 72 h of incubation at 28 °C in the dark. The formation of a growth disc in the culture medium indicated atmospheric nitrogen fixation by the bacterial strains. This procedure was repeated five times for confirmation.

Selection of endophyte phosphate solubilization

Strains that could solubilize inorganic phosphate were identified by a quantitative test. This test involved observing the presence of a halo after bacterial cultivation on medium supplemented with Ca3(PO4)2 after seven days of incubation at 28 °C. The results were quantified by estimating the halo size (cm) and dividing it by the colony size (cm) to generate a solubilization index (SI).22

Selection of endophytes that produce IAA

The strains that tested positive for phosphate solubilization and nitrogen fixation were tested for their ability to produce IAA. The quantitative IAA production was evaluated using the Patten and Glick23 method with modifications. The bacterial strains were inoculated in 10% Tryptone Soy broth medium (Difco, Livonia, USA) supplemented with L-tryptophan (5 mM) and incubated at 28 °C for 48 h in the dark. Triplicate cultures were centrifuged (5 min, 10,000 × g, at room temperature), and 1.5 mL Salkowski reagent24 was added to 1.5 mL of the supernatant. This mixture was incubated for 20 min in the dark at room temperature and analyzed using a spectrophotometer (520 nm; Ultrospec 3000, Amersham-Pharmacia Biotech). The absorbance values obtained were interpolated in a standard curve to determine the IAA concentration.

Identification of strains by partial sequencing of the 16S rDNA

The identification of 38 bacterial strains able to fix nitrogen, solubilize phosphorus and produce IAA was performed by partial sequencing of 16S rDNA. The amplification of 16S rDNA was performed directly from bacterial colonies grown on solid TSA medium (10%) (Difco, Livonia, USA) using the primers R1387 (5'-CGGTGTGTACAAGGCCCGGGAACG-3') and PO27F (GAGAGTTTGATCCTGGCTCAG-5'-3').25

The 16S rDNA gene PCR products (approximately 1500 bp) were purified by the polyethylene glycol method of Lis26 and sequenced at the Institute of the Human Genome (USP, São Paulo, Brazil). The sequences were evaluated with BLASTn27 against the database of the GenBank Development National Center for Biotechnology Information website.

Sequences were deposited in GenBank under the following access numbers: KF356429-KF356431, KF356438, KF356439, KF356444, KF356453, KF356454, KF356457, KF356459, KF356462, KF356465 and KM438481-KM438506.

Growth promotion of the seedlings of the reforestation angiosperm tree A. polyphylla

We selected two strains that presented positive results for all of the tests performed, including phosphorus solubilization, nitrogen fixation and IAA production. Both strains produced the highest amount of IAA of the bacteria studied. However, one strain had a high phosphorous solubilization index value while the other had a low value for this index. The selected bacteria were grown in liquid TSB culture medium and incubated for 24 h at 28 °C at 150 rpm. The optical density (600 nm) was adjusted to 108 cells mL-1. The plant growth assays were performed in the reforestation Bioflora Company located in Piracicaba, São Paulo, Brazil. The experiment was conducted in a plant nursery whose internal temperatures ranged from 20 to 30 °C during this period. The treatments were as follows: I, seedlings of A. polyphylla uninoculated and unfertilized; II, seedlings uninoculated and fertilized (using Forth Solúveis Inicial; Forth Aqua Micros and Forth Aqua Calcio, Tiete, Brazil); III, seedlings inoculated with Pseudomonas fluorescens (strain MCR1.10); IV, seedlings inoculated with Enterobacter sp. (strain MCR1.48); V, seedlings inoculated with P. fluorescens (strain MCR1.10) and Enterobacter sp. (strain MCR1.48) consortium. The treatments were performed in a completely randomized design with 25 repetitions (1 plant per pot). Treatments inoculated with bacteria (III, IV and V) were not fertilized. Each inoculated sample received a 1.0 mL suspension of 108 cells mL-1, and the un-inoculated controls were treated with 1.0 mL of sterile water only. The inoculation with bacteria was performed by adding the bacterial suspension to the substrate. After inoculation, the seedlings were maintained in the plant nursery for 60 days. After this period, the seedlings were collected and washed in water. Then, the root systems were separated from the shoot, and the dry mass of roots and shoots from the seedlings were measured to evaluate plant growth promotion.

Statistical analysis

Data were subjected to analysis of variance, and means were compared by the Scott Knott test for IAA production and phosphorus solubilization index (<0.05) and the Tukey Test for plant experiments (<0.05). Statistical analyses were performed with R software (version 3.0.2).

Results

Selection of endophytes: nitrogen fixation and Phosphate solubilization

In the nitrogen fixation test, 33% of the 115 strains examined (38) were able to grow in the medium free of nitrogen with a typical sub-surface growth, indicating their ability to fix nitrogen. This microaerophilic growth behavior resulted in a change of color of the culture medium from blue to green to yellow. This color change was caused by changes in the medium pH probably due to acidic molecules released by the isolate tested.

All 115 strains examined produced a halo during the phosphate solubilization test, indicating that they were all able to solubilize inorganic phosphate. The highest rates were observed in the genera Pantoea (MCL2.66), and the lowest rates were observed in the genera Sphingosinicella (MCL2.68), Xanthomonas (MCA2.20), Ochrobactrum (MBR2.28) and Bacillus (MBIA2.43, MCR2.51, MBIL2.38 and MCA2.42) with indices between 1.2 and 2.6.

Quantitative and qualitative assay of IAA production

The IAA production assay was performed in 38 strains that were able to solubilize phosphate and fix nitrogen. All of the strains tested produced IAA ranging from 16.4 to 601.7 µg mL-1. Pantoea (MCL2.66 and MBIL2.47), Enterobacter (MCL2.65, MCR1.48 and MCR1.23), Pectobacterium (MCR2.29), Bacillus (MCR2.51 and MBI2.63), Pseudomonas (MBR2.7, MCR1.10) and Stenotrophomonas (MBR2.29) genera showed the highest yields (Table 1). The lowest production was approximately 16 µg mL-1 and was produced by strains MBR2.1 and MBR2.22, corresponding to the genera Bacillus and Curtobacterium, respectively (Table 1).

Table 1 Identification and evaluation of in vitro tests for plant growth promoting bacteria isolated from branches of mangrove plants. 

Isolate Plant host Location Identification IAA (µg mL-1)a Phosphate (P) solubilization index (SI)a Nitrogen fixation
MBA2.21 Avicenia sp. Industrial contaminated (Bertioga) Bacillus pumilus 31.2d 8.0c +
MBA2.18 Avicenia sp. Industrial contaminated (Bertioga) Bacillus pumilus 27.6d 4.2e +
MBA2.16 Avicenia sp. Industrial contaminated (Bertioga) Alcaligenes sp. 33.7d 3.4e +
MBA2.4 Avicenia sp. Industrial contaminated (Bertioga) Bacillus pumilus 34.3d 4.8e +
MBA2.15 Avicenia sp. Industrial contaminated (Bertioga) Alcaligenes faecalis 32.8d 3.5e +
MBR2.45 Rhizophora mangle Industrial contaminated (Bertioga) Bacillus pumilus 24.4d 7.4d +
MBR2.40 Rhizophora mangle Industrial contaminated (Bertioga) Bacillus pumilus 32.5d 7.0d +
MBR2.28 Rhizophora mangle Industrial contaminated (Bertioga) Ochrobactrum sp. 100.3d 1.6f +
MBR2.16 Rhizophora mangle Industrial contaminated (Bertioga) Bacillus pumilus 28.3d 6.8d +
MBR2.7 Rhizophora mangle Industrial contaminated (Bertioga) Pseudomonas sp. 406.8b 8.2c +
MBR2.1 Rhizophora mangle Industrial contaminated (Bertioga) Bacillus pumilus 16.5d 5.9d +
MBR2.22 Rhizophora mangle Industrial contaminated (Bertioga) Curtobacterium flaccumfaciens 16.4d 8.2c +
MBR2.29 Rhizophora mangle Industrial contaminated (Bertioga) Stenotrophomonas sp. 443.2b 6.5d +
MBIA2.42 Avicenia sp. Oil Spill contamination (Bertioga) Bacillus amyloliquefaciens 55.4d 3.6e +
MBIA2.40 Avicenia sp. Oil Spill contamination (Bertioga) Bacillus amyloliquefaciens 48.7d 3.8e +
MBIA2.34 Avicenia sp. Oil Spill contamination (Bertioga) Pantoea agglomerans 204.2c 10.2b +
MBIA2.35 Avicenia sp. Oil Spill contamination (Bertioga) Bacillus pumilus 27.8d 8.5c +
MBIA2.43 Avicenia sp. Oil Spill contamination (Bertioga) Bacillus amyloliquefaciens 55.6d 2.6f +
MBIL2.38 Laguncularia racemosa Oil Spill contamination (Bertioga) Bacillus subtilis 56.8d 2.4f +
MBIL2.63 Laguncularia racemosa Oil Spill contamination (Bertioga) Bacillus safensis 386.6b 5.6e +
MBIL2.47 Laguncularia racemosa Oil Spill contamination (Bertioga) Pantoea dispersa 422.7b 11.2b +
MBIL2.64 Laguncularia racemosa Oil Spill contamination (Bertioga) Pantoea agglomerans 188.6c 8.4c +
MBIL2.51 Laguncularia racemosa Oil Spill contamination (Bertioga) Bacillus pumilus 21.2d 6.0d +
MBIL2.46 Laguncularia racemosa Oil Spill contamination (Bertioga) Pantoea sp. 220.4c 9.8b +
MBIR2.24 Rhizophora mangle Oil Spill contamination (Bertioga) Bacillus pumilus 27.6d 6.2d +
MBIR2.2 Rhizophora mangle Oil Spill contamination (Bertioga) Bacillus pumilus 34.8d 3.6e +
MCA2.42 Avicenia sp. Uncontaminated (Cananéias) Bacillus sp. 38.5d 2.6f +
MCL2.66 Laguncularia racemosa Uncontaminated (Cananéias) Pantoea agglomerans 513.9a 13.7a +
MCL2.65 Laguncularia racemosa Uncontaminated (Cananéias) Enterobacter ludwigii 573.9a 11.2b +
MCL2.68 Laguncularia racemosa Uncontaminated (Cananéias) Sphingosinicella sp 75.4d 1.2f +
MCA2.20 Laguncularia racemosa Uncontaminated (Cananéias) Xanthomonas campestris 86.1d 1.2f +
MCR1.48 Rhizophora mangle Uncontaminated (Cananéias) Enterobacter sp. 540a 11.1b +
MCR1.23 Rhizophora mangle Uncontaminated (Cananéias) Enterobacter sp. 477.1b 9.0lc +
MCR1.10 Rhizophora mangle Uncontaminated (Cananéias) Pseudomonas fluorescens 441.8b 6.5d +
MCR2.39 Rhizophora mangle Uncontaminated (Cananéias) Bacillus pumilus 32.4d 4.4e +
MCR2.29 Rhizophora mangle Uncontaminated (Cananéias) Peptobacterium sp. 601.7a 8.2c +
MCR2.51 Rhizophora mangle Uncontaminated (Cananéias) Bacillus safensis 402.5b 2.1f +
MCR2.33 Rhizophora mangle Uncontaminated (Cananéias) Pantoea agglomerans 124.8c 6.8d +

aIn a column, values with the same letter do not differ at a 5% significance level (Scott Knott test).

Plant growth promoting reforestation by endophytic bacteria

Two high IAA producing strains were selected. One strain had the highest phosphorous solubilization index, strain MCR1.48 (Enterobacter sp.), while strain MCR1.10 (P. fluorescens) had one of the lowest phosphorous solubilization indices. Both stains (with high and low phosphorous solubilization) were inoculated in A. polyphylla plants. The seedlings tested displayed different results when compared to the control (Table 2, Fig. 1).

Fig. 1 Monjoleiro roots under different treatments: (A) Treatment 1 (addition of only water); (B) Treatment 2 (addition of fertilizer); (C) Treatment 3 (inoculation of Pseudomonas fluorescens MCR1.10); (D) Treatment 4 (inoculation of Enterobacter sp. MCR1.48). 

Table 2 The effect of soil inoculation of endophytic mangrove bacteria on A. polyphylla after 60 days following inoculation. 

Treatment Isolate Shoot dry mass (g)a Root dry mass (g)a
Treatment 1 (adding only water) - 0.38 ± 0.04 b 0.61 ± 0.17ab
Treatment 2 (adding fertilizer) - 0.43 ± 0.09ab 0.58 ± 0.25b
Treatment 3 (inoculating MCR1.10) Pseudomonas fluorescens (MCR1.10) - low P solubilization 0.32 ± 0.05b 0.49 ± 0.16b
Treatment 4 (inoculating MCR1.48) Enterobacter sp. (MCR1.48) - high P solubilization 0.50 ± 0.06a 0.82 ± 0.53a
Treatment 5 (inoculating MCR1.10 + MCR1.48) MCR1.10 and MCR1.48 0.43 ± 0.07ab 0.65 ± 0.20ab
Average - 0.41 ± 0.13 0.62 ± 0.25

aIn a column, values with the same letter do not differ at a 5% significance level (Tukey test).

The treatment with MCR1.10 (the low phosphorous solubilization strain) as well as the consortium treatment (V) was statistically similar to the control (not inoculated and not fertilized), indicating a lack of ability to promote plant growth. In contrast, seedlings inoculated with MCR1.48 (high phosphorous solubilization strain) caused an increase in dry root and shoot biomass. Some of the treated seedlings had an increase in the number of root hairs in their adventitious roots along with root page thickening when compared with the control (Table 2, Fig. 1). The seedlings with MCR1.48 (high phosphorous solubilization strain) inoculum and associated with fertilization were statistically similar.

Discussion

The ability of endophytic bacteria to promote plant growth is attributed to direct mechanisms such as nitrogen fixation, plant hormone production (mainly IAA),28 phosphate solubilization, and siderophore production29 as well as the increased absorption of water and nutrients and the suppression of deleterious microorganisms by metabolite production.30

Phosphate solubilization by microorganisms has an important function in supplying phosphorus (P) to plants31 with the potential to be used as inoculants. Many authors32 report that the ability of microorganisms to solubilize phosphate correlates with the ability to produce organic acids and/or extracellular polysaccharide.33-35 In this study, all of the endophytes tested could solubilize P in vitro, although their solubilization indices varied.

In the IAA production assay, strains produced similar or higher amounts of IAA than previous reports indicated36,37 demonstrating their potential to improve plant growth. Some strains of the same species had different values, possibly due to the different amounts of tryptophan in the strains with each cell line having an optimal concentration for interfering with the synthesis of IAA.38,39

Several studies report that endophytic microorganisms are able to promote plant growth in different plants,17,40 such as corn (Zea Mays), peppermint (Mentha piperita),41Vitis vinifera L.42 and pineapple (Ananas comosus).43 However, there are few reports on the use of endophytic bacteria on growth promotion in woody plants. Previous studies were performed mainly on trees important in the paper and cellulose industry, such as pines and eucalyptus.18 Burns and Schwarz44 found that an unidentified bacterium induced 90% of root explant in Pinus elliottii when compared to the control.44 Other tree species studied included poplar (Populus), which was used as a model plant, and the bacterium Burkholderia multivorans, a nonpathogenic strain, reported to be able colonize the roots and significantly promote the growth of poplar seedlings.45 Moreover, Bashan46 reported that Azospirillum brasilense and Bacillus pumilus promoted the growth of two trees used in reforestation (Prosopis articulata and Parkinsonia microphylla), thus increasing plant development and survival.

Two high IAA producing strains, P. fluorescens and Enterobacter sp., were selected to inoculate plants and evaluate their growth in field conditions since both the Pseudomonas and Enterobacter genera have been previously reported to promote the growth of plants.47-49 The main difference between the strains selected is that Enterobacter sp. (MCR1.48) has a high P solubilization index, while P. fluorescens (MCR1.10) has one of the lowest P solubilization indices. We selected the commonly used reforestation tree A. polyphylla, which has few published studies involving inoculation by the bacteria of interest. The present work demonstrates that the inoculation of a high P solubilization strain MCR1.48 (Enterobacter sp.) increased the shoot dry mass of monjoleiro. This result indicates that phosphorous solubilization plays a key role in tree plant growth. Therefore, this study is the first known report of the growth promotion of A. polyphylla from the inoculation of an endophytic bacterium.

In reforestation, the rotation time is long, so the inoculation of microorganisms to accelerate the trees' development should help them grow more quickly over the long term. We report an increase in the rate of seedling survival, early establishment in the field after planting along with improvement in quality and characteristics of the root system, i.e., all advantages desirable for better production. Therefore, we successfully screened the biotechnological potential of endophyte isolates from mangroves, focusing on plant growth promotion. We selected a strain able to provide limited nutrients by fixing nitrogen since not all forms of nitrogen in the soil are available to the plant; by solubilizing phosphorus, which is present in soil but is also not available to plants; and by producing plant hormones involved in plant growth such as auxin (IAA).

The use of an endophytic microorganism in the substrate as inoculum for the production of seedlings of A. polyphylla is a highly successful strategy that can be utilized in the reforestation nursery. The inoculant effect of the Enterobacter sp. endophytic bacteria in A. polyphylla seedlings affect the quality of seedling production, improving the development and possibly reducing the cost of chemical fertilization performed by the nursery company.

Acknowledgments

This work was supported by a grant from FAPESP (Proc.2004/15414-6) to R.A. and by a fellowship from CNPq to M.N.D. (Proc 150228/2017-1). We thank Bioflora for providing the structure and staff to develop nursery experiments.

References

1 Alongi DM. Present state and future of the world's mangrove forest. Environ Conserv. 2002;3:331-349. [ Links ]

2 Zhou HW, Guo CL, Wong YS, Tam NFY. Genetic diversity of dioxygenase genes in polycyclic aromatic hydrocarbon-degrading bacteria isolated from mangrove sediments. FEMS Microbiol Lett. 2006;262:148-157. [ Links ]

3 Dias AC, Andreote FD, Dini-Andreote F, et al. Diversity and biotechnological potential of culturable bacteria from Brazilian mangrove sediment. World J Microbiol Biotechnol. 2009;25:1305-1311. [ Links ]

4 Holguin AG, Gonzalez-Zamorano BP, Bashan ALE, Mendoza AR, Amador AE, Bashan AY. Mangrove health in an arid environment encroached by urban development—a case study. Sci Total Environ. 2006;363(1):260-274. [ Links ]

5 Thatoi H, Behera BC, Mishra RR, Dutta SK. Biodiversity and biotechnological potential of microorganisms from mangrove ecosystems: a review. Ann Microbiol. 2013;63:1-19. [ Links ]

6 Andreote FD, Jiménez DJ, Chaves D, et al. The microbiome of Brazilian mangrove sediments as revealed by metagenomics. PLoS ONE. 2012;7:6. [ Links ]

7 Dias AC, Pereira e Silva Mde C, Cotta SR, et al. Abundance and genetic diversity of nifH gene sequences in anthropogenically affected Brazilian mangrove sediments. Appl Environ Microbiol. 2012;78(22):7960-7967. [ Links ]

8 Varon-Lopez M, Dias AC, Fasanella CC, et al. Sulphur-oxidizing and sulphate-reducing communities in Brazilian mangrove sediments. Appl Environ Microbiol. 2014;16(3):845-855. [ Links ]

9 de Souza Sebastianes FL, Romão-Dumaresq AS, Lacava PT, et al. Species diversity of culturable endophytic fungi from Brazilian mangrove forests. Curr Genet. 2013;59(3):153-166. [ Links ]

10 Castro RA, Quecine MC, Lacava PT, et al. Isolation and enzyme bioprospection of endophytic bacteria associated with plants of Brazilian mangrove ecosystem. Springerplus. 2014;3:382. [ Links ]

11 Paz ICP, Santin RCM, Guimarães AM, et al. Eucalyptus growth promotion by endophytic Bacillus spp. Genet Mol Res. 2012;11:3711-3720. [ Links ]

12 Quecine MC, Araujo WL, Rossetto PB, et al. A sugarcane growth promotion by the endophytic bacterium Pantoea agglomerans 33.1. Appl Environ Microbiol. 2012;78:7511-7518. [ Links ]

13 Habibi S, Djedidi S, Prongjunthuek K, et al. Physiological and genetic characterization of rice nitrogen fixer PGPR isolated from rhizosphere soils of different crops. Plant Soil. 2014;379(1-2):51-66. [ Links ]

14 Pardos M, Climent J, Almeida H, Calama R. The role of developmental stage in frost tolerance of Pinus pinea L. seedlings and saplings. Ann For Sci. 2014;71:551-562. [ Links ]

15 Doty SL. Enhancing phytoremediation through the use of transgenics and endophytes. New Phytol. 2008;79(2):318-333. [ Links ]

16 Dourado MN, Ferreira A, Araújo WL, Azevedo JL, Lacava PT. The diversity of endophytic methylotrophic bacteria in an oil-contaminated and an oil-free mangrove ecosystem and their tolerance to heavy metals. Biotechnol Res Int. 2012;:1-8. [ Links ]

17 Hardoim PR, van Overbeek LS, Berg G, et al. The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiol Mol Biol. 2015;79:293-320. [ Links ]

18 Chanway CP. Inoculation of tree roots with plant growth promoting soil bacteria: an emerging technology for reforestation. Forest Sci. 1997;43(14):99-112. [ Links ]

19 Chaer GM, Resende AS, Campello EFC, de Faria SM, Boddey RM. Nitrogen-fixing legume tree species for the reclamation of severely degraded lands in Brazil. Tree Physiol. 2011;31(2):139-149. [ Links ]

20 Rao KPC, Verchot LV, Joshi LM. Adaptation to climate change through sustainable management and development of agroforestry systems. J SAT Agric Res. 2007;4(1):1-30. [ Links ]

21 Liba CM, Ferrara FIS, Manfio GP, et al. Nitrogen-fixing chemo-organotrophic bacteria isolated from cyanobacteria-deprived lichens and their ability to solubilize phosphate and to release amino acids and phytohormones. J Appl Microbiol. 2006;101:1076-1086. [ Links ]

22 Verma SC, Ladha JK, Tripathi AK. Evaluation of plant growth promoting and colonization ability of endophytic diazotrophs from deep water rice. J Biotechnol. 2001;91:127-141. [ Links ]

23 Patten CL, Glick BR. Role of Pseudomonas putida indole acetic acid in development of the host plant root system. Appl Environ Microbiol. 2002;68:3795-3801. [ Links ]

24 Bric JM, Bostock RM, Silverstone S. Rapid in situ assay for indolacetic acid production by bacteria immobilized on nitrocellulose membrane. Appl Environ Microbiol. 1991;57:535-538. [ Links ]

25 Heuer H, Krsek M, Baker P, Smalla K, Wellington EMH. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Appl Environ Microbiol. 1997;63:3233-3241. [ Links ]

26 Lis JT. Fractionation of DNA fragments by polyethylene glycol induced precipitation. Methods Enzymol. 1980;65:347-353. [ Links ]

27 Altschul SF, Gish W, Miller W, Myers EW. Basic local alignment search tool. J Mol Biol. 1990;215:403-410. [ Links ]

28 Andrade LF, de Souza GL, Nietsche S, et al. Analysis of the abilities of endophytic bacteria associated with banana tree roots to promote plant growth. J Microbiol. 2014;52:27-34. [ Links ]

29 Glick BR. The enhancement of plant growth by free-living bacteria. Can J Microbiol. 1995;41(2):109-117. [ Links ]

30 Brader G, Compant S, Mitter B, Trognitz F, Sessitsch A. Metabolic potential of endophytic bacteria. Curr Opin Biotechnol. 2014;27:30-37. [ Links ]

31 Richardson AE, Barea JM, McNeill AM, Prigent-Combaret C. Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil. 2009;321:305-309. [ Links ]

32 Kim KY, Jordan D, Donald GAM. Effect of phosphate-solubilizing bacteria and vescular-arbuscular mycorrhizae on tomato growth and soil microbial activity. Biol Fert Soils. 1998;26:79-87. [ Links ]

33 Sharma SB, Sayyed RZ, Trivedi MH, Gobi TA. Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus. 2013;2:587. [ Links ]

34 Souchie EL, Saggin-Júnior OJ, Silva EMR, Campello EFC, Azcón R, Barea JM. Communities of P-solubilizing bacteria, fungi and arbuscular mycorrhizal fungi in grass pasture and secondary forest of Paraty, RJ-Brazil. An Acad Bras Cienc. 2006;78:1-11. [ Links ]

35 Wakelin SA, Warren RA, Harvey PR, Ryder MH. Phosphate solubilization by Penicillium spp. closely associated with wheat roots. Biol Fert Soils. 2004;40:36-43. [ Links ]

36 Ali B, Sabri AN, Ljung K, Hasnain S. Quantification of indole-3-acetic acid from plant associated Bacillus spp. and their phytostimulatory effect on Vigna radiata (L.). World J Microbiol Biotechnol. 2009;25:519-526. [ Links ]

37 Ravari SB, Heidarzadeh N. Isolation and characterization of rhizosphere auxin producing Bacilli and evaluation of their potency on wheat growth improvement. Arch Agron Soil Sci. 2014;60(7):895-905. [ Links ]

38 Bar T, Okon Y. Tryptophan conversion to indole-3-acetic acid via indole-3-acetamide in Azospirillum brasilense Sp 7. Can J Microbiol. 1993;39:81-86. [ Links ]

39 Pastor N, Rosas S, Luna V, Rovera M. Inoculation with Pseudomonas putida PCI2, a phosphate solubilizing rhizobacterium, stimulates the growth of tomato plants. Symbiosis. 2014;62(3):157-167. [ Links ]

40 Santoyo G, Moreno-Hagelsieb G, Orozco-Mosqueda MC, Glick BR. Plant growth-promoting bacterial endophytes. Microbiol Res. 2016;183:92-99. [ Links ]

41 Mucciarelli M, Scannerini S, Bertea C, Maffei M. In vitro and in vivo peppermint (Mentha piperita) growth promotion by nonmycorrhizal fungal colonization. New Phytol. 2003;158:579-591. [ Links ]

42 Compant S, Reiter B, Sessitsch A, Nowak J, Clément C, Barka EA. Endophytic colonization of Vitis vinifera L. by plant growth-promoting bacterium Burkholderia sp. strain PsJN. Appl Environ Microbiol. 2005;71:1685-1693. [ Links ]

43 Baldotto MAB, Canellas LP, Bressan-Smith R, Olivares FL. Growth promotion of pineapple 'vitória' by humic acids and Burkholderia spp. during acclimatization. Rev Bras Ciênc do Solo. 2010;5:1593-1600. [ Links ]

44 Burns JA, Schwarz OJ. Bacterial stimulation of adventitious rooting on in vitro cultured slach pine (Pinus elliottii Engelm.) seedling explants. Plant Cell Rep. 1996;15(6):405-408. [ Links ]

45 Li GX, Wu XQ, Ye JR. Biosafety and colonization of Burkholderia multivorans WS-FJ9 and its growth-promoting effects on poplars. Appl Microbiol Biotechnol. 2013;97(24):10489-10498. [ Links ]

46 BashanBashan Y, Salazar B, Puente ME, Bacilio M, Linderman R. Enhanced establishment and growth of giant cardon cactus in an eroded field in the Sonoran Desert using native legume trees as nurse plants aided by plant growth-promoting microorganisms and compost. Biol Fert Soils. 2009;6:585-594. [ Links ]

47 Duan J, Jiang W, Cheng Z, Heikkila JJ, Glick BR. The complete genome sequence of the plant growth-promoting bacterium Pseudomonas sp. UW4. PLoS ONE. 2013;8(3):e58640. [ Links ]

48 Rajkumar M, Freitas H. Influence of metal resistant-plant growth-promoting bacteria on the growth of Ricinus communis in soil contaminated with heavy metals. Chemosphere. 2008;71(5):834-842. [ Links ]

49 Wang YL, Yang X, Zhang X, et al. Improved plant growth and Zn accumulation in grains of rice (Oryza sativa L.) by inoculation of endophytic microbes isolated from a Zn Hyperaccumulator, Sedum alfredii H. J Agric Food Chem. 2014;62(8):1783-1791. [ Links ]

Received: August 23, 2016; Accepted: April 19, 2017

*Corresponding author at: Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Av. Prof. Lineu Prestes, 1374 - Ed. Biomédicas II, 05508-900 Piracicaba, SP, Brazil. E-mail: mndourado@gmail.com (M.N. Dourado).

Conflicts of interest

The authors declare no conflicts of interest.

Associate Editor: Welington Araújo

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