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Print version ISSN 1517-8692
Rev Bras Med Esporte vol.10 no.5 Niterói Sept./Oct. 2004
Niveles de triglicéridos intra y extracelulares en músculos humanos mediante 1H-ERM Estudio de caso
Maria Gisele dos SantosI; Iverson ladewigI; Raul OsieckiI; André GomesII; Jorge Andrés CalvarIII
Education Department, UFPR
IIAdvanced Image Diagnostic Center, DAPI
IIIMagnetic Resonance Service, FLENI (Argentina)
The objective of this study was to analyze the intra (IT) and extra cellular (ET) triglycerides consumption in the soleus, tibialis anticus, vastus internus muscles, after 4 hours training ride on the road. This study used a top-level cyclist as a case study. Magnetic resonance studies used the following spectroscopy parameters imposed to adjust the time, such as the distance of the frequency between the signs IT and ET of the similar chemical nature. We were able to conclude that the vastus internus was the muscle which demonstrated the largest triglyceride consumption as the energetic substrate after a 4 hour bike ride on the road. Thus, we can infer that a workout at 80% of the VO2max enabled the consumption of intra muscle triglyceride during exercise.
Key words: Cyclist. Triglycerides. Magnetic resonance.
El objetivo de este estudio fue el de analizar el consumo de triglicéridos intra (IT) y extracelulares (ET) en los músculos soleo, tibial anterior y vasto medial tras una prueba de cuatro horas de ciclismo de ruta. Esta investigación es un estudio de caso de un ciclista que participa de competiciones internacionales. Los estudios de resonancia magnética utilizaron los siguientes parámetros espectrales que se impusieron para el ajuste en el dominio del tiempo, como la distancia de las frecuencias entre las señales de IT e ET. Los valores de amplitudes de los triglicéridos intra y extracelulares fueron divididos por resonancia de agua. Concluimos que el músculo vasto medial del ciclista presentó mayor consumo de triglicéridos luego de cuatro horas de ciclismo en ruta. Por lo tanto, se verificó que un trabajo con intensidad del 80% de la frecuencia cardiaca máxima permitió el consumo de triglicéridos intramusculares durante el ejercicio.
Palabras-clave: Ciclismo. Triglicéridos intramusculares. Resonancia magnética.
The constant advance of results of the high-level sportive practice is grounded on the improvement and optimization of the athlete's motricity based on a series of environmental, biomechanical, psychological and physiological factors. The interest of the study on physiological factors includes the direct or indirect stimulation of the energy stores as well as their availability, transportation and utilization during exercise.
We know that the hydrolysis of the adenosine-triphosphate (ATP) is required for the muscle contraction to occur and that the aerobic and anaerobic processes will deplete the substrates that will allow maintaining the ATP level required to perform repeated contractions. During many years, the only way to valorize the biochemical and exercise-induced physiological modifications consisted of analyzing the cardiovascular and metabolic adaptation kinetic (cardiac frequency, oxygen intake, lactatemia, etc.). However, a new methodology called as nuclear magnetic resonance (NMR) has recently emerged, what allows the direct study of the biochemical modifications produced in the muscle during contraction.
The NMR functions as a real local chamber that enables visualizing in a non-invasive way the variation of the elements that influence the ATP resynthesis. This non-invasive featured NMR allows performing an unlimited number of measurements, what favors the attainment of data with physiological interest including situations close to important competitions. As result, the use of this new methodology to valorize the physiological intramuscular modifications produced during exercise and during the recovery phase opens new horizons for the functioning comprehension of the most intimate mechanisms of the muscular function.
The magnetic resonance (MR) methods are more and more used to investigate the physiology of the human muscle. Although the MR image (MRI) reveals the muscle volume and the orientation of its fibers, the MR spectroscopy (MRS) provides information with regard to the tissue chemical composition. Depending on the nucleus observed, the MRS allows the observation of phosphorylated metabolites involved in the muscular bioenergetics (31P-MRS), glycogen (13C-MRS) or intramyocellular lipids (1H-MRS).
The triglycerides metabolism in the fat tissue and its regulation has been studied in details for many years. However, the knowledge of the triglycerides (TG) metabolism regulation in muscle is limited.
However, it was estimated that the contribution of the muscular TG for the total production of energy during exercise (65% of the O2max) was 15-35%(1-4). The energetic contribution of distinct substrates during exercise at three distinct intensities was also investigated(5). The intramuscular TG contribution was of 7%, 26% and 8% during exercise performed at 25%, 65% and 85% of the O2max., respectively. This suggests that there is an optimal point in the utilization of intramuscular TG between 25% and 85% of the O2max. Highly trained athletes seem to use more their intracellular triglycerides (IT) stores. In this context, other study demonstrated that the training increases the contribution of the IT consumption to the total energetic expenditure(6).
Generally, one may conclude that the muscular TG are consumed during the submaximal exercise as an important substrate for the contracting muscle(7). It was also recently described that the intra (IT) and extra (ET) muscular triglycerides may be quantified in a non-invasive and non-destructive way by proton magnetic resonance spectroscopy (1H-MRS)(4,8-13). One may observe some advantages in the utilization of the magnetic resonance non-destructive method in the biopsy, once it presents higher volume of accessible sample, uses the same investigated zone before and after exercise, allows the distinguishing between IT and ET and, finally, enables higher access to elite athletes, who do not easily agree with the performance of tests by means of biopsies.
Therefore, this study had as objective analyzing the intra and extra muscular triglycerides levels in the soleus, tibialis anticus, vastus internus muscles through proton magnetic resonance spectroscopy before and after four hours of training ride on the road.
This research is characterized as case study with an international level cyclist who had been previously informed about the type of experiment he would participate in and signed the consent form. The protocols and the consent form were previously approved by the Ethics Commission of the Federal University of Paraná.
Magnetic resonance (MR) procedures for 1H-MRS
The athlete arrived in the magnetic resonance unit of the Diagnostic and Image Center (DAPI) two hours after his last meal. The magnetic resonance studies were performed in a superconductor magnet that generates a magnetic field of 1.5 Tesla by means of the utilization of a knee coil (figure 1).
During the experimental protocol, the athlete remained lying at supine position with his right leg immobilized and aligned with the magnet longitudinal axis. The left leg was supported with cushions out of the coil. A mark on the skin was performed and the distance from the mark up to the patella was measured with a tape measure to know the correct distance in the medium portion of the following muscles: soleus (S), tibialis anticus (TA), vastus internus (VI) in order to have the same region as reference for the performance of the experimental protocol before and after four hours training ride on the road and this mark was positioned at the magnet isocenter.
After positioning, the athlete was reminded not to move while the protocol of 1H-MRS was performed (figure 2).
The spectra were processed using the Magnetic Resonance User Interface (MRUI) software, which is a mathematic program that enables the application of algorithms of signals in time domain for the extraction of the spectra parameters that may express biochemical information.
The spectral parameters imposed for the adjustment in time domain were: the frequency distance between IT and ET signals from protons (-CH2-)n or -CH3, which was assumed as cause of the magnetic susceptibility effects(8-11), being considered as equivalent for both resonances (dET,CH2 dIT,CH2 = dET,CH3 dIT,CH3). However, the chemical dislocations interval for groups -CH3-, (-CH2-)n of extra cellular triglycerides (ET), =C-CH2-, and intra cellular triglycerides (IT) HOOC-CH2-, were restricted to intervals between 1.0-1.2, 1.4-1.7, 1.7-1.9 and 2.3-2.5 ppm, respectively. The smoothing factors (R) and the amplitudes (a) were fixed as RET,CH3 = 0.842 RET,CH2 and RIT,CH3 = 0.842 RIT,CH2, and aET,CH3 = 0.130 aET,CH2 and aIT,CH3 = 0.124 aIT,CH2, respectively.
The phase correction of order zero and one was estimated by AMARES in the MRUI program. However, the relative phase of the resonances between each other remained zero. The water resonance was quantified by AMARES and the water FID without being suppressed was obtained at the same voxel using the sine curve exponentially smoothed (corresponding to a Lorentzian signal in the frequency domain). In this last case, all adjustment parameters were left with no restriction. The areas calculated were corrected by the differential saturation effects using the longitudinal (T1) and transversal (T2) relaxation times of the resonances of interest in lipids and water (figure 3).
Table 1 presents the results of the intra and extra cellular triglycerides of soleus, tibialis anticus, vastus internus muscles. A higher consumption of intracellular triglycerides was observed after training ride on the road.
The possible alterations of the intra and extracellular triglyceride concentration in the soleus, tibialis anticus, vastus internus muscles after four hours of training ride on the road in a highly trained athlete were investigated through non-invasive measurements in this part of the study. The results demonstrated IT consumption of 63% in the vastus internus muscle after four hours of training ride.
The IT metabolism was studied during continuous exercises of several intensities and durations by means of biopsies. The different results showed disagreements with regard to the use of IT during exercise. Thus, the IT contribution in the energetic metabolism of the vastus internus muscle in humans during intermittent exercises was evaluated(14,15). Researchers reported a decrease on IT of approximately 25% of the rest value after 60 minutes of intermittent bike exercise, alternating 15 s at 100% of the O2max with 15 s of recovery(14). After 15 minutes of exercise, these authors observed IT decreasing tendency. In this context, a decrease of 29% in IT after 30 s of exercises performed until fatigue alternated with 5 minutes of recovery was also observed(15).
The utilization of IT and ET in soleum, gastrocnemius and tibialis anticus muscles in humans was studied by means of 1H-MRS in two high-intensity continuous exercise protocols alternated with periods of lower intensity(10).
A consumption of IT and ET during exercise protocols performed was not found in this study. However, some researchers described IT depletion ranging from 20 to 50% of the rest value in exercises performed from 55 to 65% of the O2max(1,2,6,16,17). In this regard, a decrease of 40% in IT was found through 1H-MRS in a subject after 3 hours pedaling mountain bike(4,9). However, our study is in agreement with studies previously mentioned because it presented consumption of 63% of IT in the vastus internus muscle after four hours of training ride on the road at intensity of 80% of the maximal cardiac frequency.
Contrarily to these results, other researchers reported no alterations in IT concentrations during bike exercise of 25 to 120 minutes at intensity of 50 to 65% of the O2max or leg extension dynamic exercises at intensity of 68% of the O2(18-22). However, it has been demonstrated that the highest utilization of IT occurs at intensity of 65% of the O2max(17).
An additional reason for the disagreements existing in literature with regard to the utilization of the IT during exercises is the existence of possible methodological problems. A variation coefficient of 35% was observed in IT values in prepared sample biopsies combining with the same type of fibers (fibers I or II)(22), however, other study observed variation coefficient of 24% in skeletal muscle biopsies(21).
The main problem of the utilization of biopsy analysis methodologies seems to be related to the difficulty in separating the ET situated next to the muscular fibers through dissection methods. On the other hand, using 1H-MRS, the variation coefficient described for the IT determination in the different studies was of 6%(9), 14%(10) and 12%(11).
Several studies proposed that an important factor to detect IT muscular alterations is the exercise protocol duration(14,19,20,24,25). In this regard, it was observed by means of biopsies that during extended exercises of up to seven to eight hours, a decrease of 53% to 63% on IT(26,27) occurs. This IT depletion occurs when the muscular glycogen or the surrounding glucose diminishes with the progressive exercise protocol duration(28,29).
Another important cause of the IT decrease is the intensity of the exercise performed(5,29,30). Studies in animal model verified reduction of IT during intermittent contractions of 30 s at 5 Hz followed 60 s of rest in female rat muscle(29,30). However, results in humans verified that the peripheral lipolysis was stimulated when subjects worked at 25% of the O2max and the IT consumption was stimulated at intensities between 65% and 85% of the O2max(5).
During short and intense exercises, it seems clear that IT consumption is not detected and, therefore, the energetic supply is mainly provided from the PCr and glycogen hydrolysis in both types of muscular fibres(31,32).
However, we cannot disregard a possible contribution of the surrounding fatty acids and those released by the lipoprotein lipase from endothelial capillaries to the muscular energetic metabolism during exercise protocol(25,33). Thus, the results obtained by means of the palmitate marking with 14C demonstrate an active turnover of the IT pool when exercises at 45% of the O2max are performed with depletion and resynthesis quantitatively comparable resulting in IT pool apparently constant(34).
The intra (IT) and extracellular (ET) triglycerides in the soleus, tibialis anticus, vastus internus muscles of a cyclist were studied by means of 1H-MRS in a non-invasive way.
We concluded that the vastus internus muscle of the cyclist presented higher triglyceride consumption after four hours of training ride on the road; these results are in agreement with a biomechanical analysis that reports that the vastus internus muscle is one of the main muscles responsible for the movement performed by the cyclist. Therefore, one concludes that a work with intensity of 80% of the maximal cardiac frequency allowed triglyceride consumption during exercise.
All the authors declared there is not any potential conflict of interests regarding this article.
1. Hurley JF, Nemeth PM, Martin WH, Hagberg JM, Dalsky GP, Holloszy JO. Muscle triglyceride utilization during exercise: effect of training. J Appl Physiol 1986; 60:562-7. [ Links ]
2. Jansson E, Kaijer L. Substrate utilization and enzymes in skeletal muscle of extremely endurance-trained men. J Appl Physiol 1987;62:999-1005. [ Links ]
3. White LJ, Ferguson MA, McCoy SC, Kim H. Intramyocellular lipid changes in men and women during aerobic exercise: a 1H-magnetic resonance spectroscopy study. J Clin Endocrinol Metabol 2003;88:5638-43. [ Links ]
4. Schrauwen-Hinderling VB, Schrauwen P, Hesselink MKC. The increase in intramyocellular lipid content is a very early response to training. J Clin Endocrinol Metab 2003;88:1610-6. [ Links ]
5. Romjin JA, Coyle EF, Sidossis LS, Gastaldelli A, Horowitz JF, Endert E, et al. Regulation of endogenous fat and carbohydrate metabolism in relation to exercise intensity and duration. Am J Physiol 1993;265:E380-91. [ Links ]
6. Johnson NA, Stannard SR, Mehalski K, Trenell MI. Intramyocellular triacylglycerol in prolonged cycling with high and low carbohydrate availability. J Appl Physiol 2003;94:1365-72. [ Links ]
7. Jensen MD. Fate of fatty acids at rest and during exercise: regulatory mechanisms. Acta Physiol. Scand 2003;178:385-90. [ Links ]
8. Schick F, Eismann B, Jung WI, Bongers H, Bunse M, Lutz O. Comparison of localized proton NMR signals of skeletal muscle and fat tissue in vivo: two lipid compartments in muscle tissue. Magn Reson Med 1993;29:158-67. [ Links ]
9. Boesch C, Slotboom H, Hoppeler H, Kreis R. Observation of mobilization and recovery of intra-myocellular lipids 1H-MRS. Magn Reson Med 1997;37:484-93. [ Links ]
10. Rico-Sanz J, Hajnal JJV, Thomas EL, Mierisova S, Ala-korpela M, Bell JD. Intracellular and extracellular skeletal muscle triglyceride metabolism during alternating intensity exercise in humans. J Physiol 1998;510:615-22. [ Links ]
11. Szczepaniak LS, Babcock EE, Schick F, Dobbins RL, Garg A, Burns DK, et al. Measurement of intracellular triglyceride stores by 1H spectroscopy: validation in vivo. Am J Physiol 1999;276:E977-89. [ Links ]
12. Watt MJ, Heigenhauser GJ, Spriet LL. Intramuscular tri acylglycerol utilization in human skeletal muscle during exercise: is there a controversy? J Appl Physiol 2002;93:1185-95. [ Links ]
13. Lange KH. Fat metabolism in exercise with special reference to training and growth hormone administration. Scand J Med Sci Sports 2004;14:74-99. [ Links ]
14. Essen B, Hagenfeldt L, Kaijser L. Utilization of blood-borne and intramuscular substrates during continuous and intermittent exercise in man. J Physiol 1977; 265:489-506. [ Links ]
15. Essen-Gustavsson B, Tesch PA. Glycogen and triglyceride utilization in relation to muscle metabolic characteristics in men performing heavy-resistance exercise. Eur J Appl Physiol 1990;61:5-10. [ Links ]
16. Cleroux JP, Van Nguyen P, Taylor AW, Leenen FHH. Effects of B1 vs B1+B2 blockade on exercise endurance and muscle metabolism in humans. J Appl Physiol 1989;66:548-54. [ Links ]
17. Guo Z, Mishra P, Macura S. Sampling the intramyocellular triglycerides from skeletal muscle. J Lipid Res 2001;42:1041-8. [ Links ]
18. Standl E, Lotz N, Dexel T, Janka HU, Kolb HJ. Muscle triglycerides in diabetic subjects. Diabetologia 1980;18:463-9. [ Links ]
19. Jansson EY, Kaijer L. Effect of diet on the utilization of blood-borne and intramuscular substrates during exercise in man. Acta Physiol Scand 1982;115:19-30. [ Links ]
20. Kiens B, Essen-Gustavson B, Christensen NJ, Saltin B. Skeletal muscle substrate utilization during submaximal exercise in man: effect of endurance training. J Physiol 1993;469:459-78. [ Links ]
21. Wendling PS, Peters SJ, Heigenhauser GJF, Spriet L. Variability of triacylglycerol content in human muscle biopsy samples. J Appl Physiol 1996;81:1150-5. [ Links ]
22. Starling RD, Trappe TA, Parcell AC, Kerr CG, Fink WJ, Costill DL. Effects of diet on muscle triglyceride and endurance performance. J Appl Physiol 1997;82:1185-9. [ Links ]
23. Essen B, Jansson E, Henriksson J, Taylor A, Saltin B. Metabolic characteristics of fibre types in human skeletal muscle. Acta Physiol Scand 1975;95:153-65. [ Links ]
24. Havel RJ, Carlson LA, Ekelund LG, Holmgren A. Turnover rate and oxidation of different free fatty acids in man during exercise. J Appl Physiol 1964;23:90-9. [ Links ]
25. Havel RJ, Pernow B, Jones NL. Uptake and release of free fatty acids and other metabolites in the legs of exercising men. J Appl Physiol 1967;23:90-9. [ Links ]
26. Froberg SO, Mossfeldt F. Effect of prolonged strenuous exercise on the concentration of triglycerides, phospholipids and glycogen in muscle of man. Acta Physiol Scand 1971;82:167- 71. [ Links ]
27. Lithell HJ, Orlander R, Schele R, Sjodin B, Karlsson J. Changes in lipoprotein-lipase activity and lipid stores in human skeletal muscle with prolonged heavy exercise. Acta Physiol Scand 1979;107:257-61. [ Links ]
28. Stankiewicz-Choroszucha B, Gorski J. Effect of decreased availability of substrates on intramuscular triglyceride utilization during exercise. Eur J Appl Physiol Occup Physiol 1978;40:27-35. [ Links ]
29. Hopp JF, Palmer WK. Electrical stimulation on intracellular triacylglycerol skeletal muscle. J Appl Physiol 1990;68:2473-81. [ Links ]
30. Hopp JF, Palmer WK. Effect of electrical stimulation on intracellular triacylglycerol in isolated skeletal muscle. J Appl Physiol 1990;68:348-54. [ Links ]
31. Gollnick PD, Piehl K, Santil B. Selective glycogen depletion pattern in human muscle fibers after exercise of varying intensity and at varying pedaling rates. J Physiol 1974;241:45-57. [ Links ]
32. Guo Z. Triglyceride content in skeletal: variability and the source. Anal Biochem 2001;296:1-8. [ Links ]
33. Mackie BG, Dudley GA, Kaciuba-Uscilko H, Terjung L. Uptake of chylomicron triglycerides by contracting skeletal muscle in rats. J Appl Physiol 1980;49:851-5. [ Links ]
34. Guo Z, Burguera B, Jensen MD. Kinetics of intramuscular triglyceride fatty acids in exercising humans. J Appl Physiol 2000;89:2057-64. [ Links ]
Maria Gisele dos Santos
Universidade Federal do Paraná, Setor de Ciências Biológicas, Departamento de Educação Física
Rua Coração de Maria, 92 BR 116, km 95, Jardim Botânico
80215-370 Curitiba, PR, Brazil
Tel.: (41) 360-4325. Fax: (41) 360-4336
Received in 20/4/04. 2nd version received in 16/6/04. Approved in 3/7/04.