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On-line version ISSN 1806-9940
Rev Bras Med Esporte vol.11 no.2 Niterói Mar./Apr. 2005
La suplementación de creatina anula el efecto adverso del ejercicio de endurance sobre el subsequente desempeño de fuerza
Rodrigo Vitasovic GomesI; Marcelo Saldanha AokiI, II
IExercise Physiology Laboratory
UniFMU University Center, São Paulo, Brazil
IIBiomedics Sciences Institute University of São Paulo, São Paulo, Brazil
OBJECTIVE: The objective of this study
was to verify if creatine supplementation exerts an ergogenic effect during
METHODS: Sixteen female university students were divided into two groups: placebo (P) and creatine supplemented (CRE). The participants received 20 g of placebo or creatine for five days and 3 g for the following seven days in a double-blind design. Before supplementation, the participants were submitted to a 1-RM test in the leg press followed by maximum repetition test (three sets of repetitions-to-fatigue, performed at 80% of the 1-RM and separated from 150 seconds of recovery P 1st set: 9.0 ± 2.4; 2nd set: 8.9 ± 2.9 and 3rd set: 8.3 ± 3.3 and CRE 1st set: 10.2 ± 2.2; 2nd set: 9.8 ± 2.9 e 3rd set: 9.7 ± 3.5 reps). After 12 days of supplementation, the participants were submitted to aerobic test in which they were instructed to cover the maximal distance as possible in 20 min. Subsequently, the participants were submitted to 1-RM test once again followed by the maximum repetition test.
RESULTS: No differences were observed in the aerobic task performance and in the 1-RM test. After the aerobic test, a decline on the repetition maximum capacity was observed during the last two sets in P (Reps - P: 1st set: 7.6 ± 2.6; 2nd set: 4.3 ± 2.9*; p < 0.01 and 3rd set: 4.6 ± 2.3*; p < 0.01). This reduction was not observed in CRE (Reps - CRE: 1st set: 10.9 ± 2.9; 2nd set: 9.5 ± 2.7 and 3rd set: 9.0 ± 3.0).
CONCLUSIONS: There is a hypothesis that the performance of resistance exercise is reduced by a residual fatigue from the previous aerobic exercise bout. One of the peripheral causes of acute fatigue during resistance exercise is related to creatine-phosphate depletion. Probably, the supplementation-induced greater muscle creatine-phosphate content accelerates the recovery and the ATP re-phosphorylation, serving as an additional energetic substrate during concurrent exercise.
Key words: Creatine. Supplementation. Concurrent exercise. Interference.
OBJETIVO: El presente estudio tiene
como objetivo verificar el efecto ergogénico que ejerce la suplementación
de la creatina durante la ejecución del ejercicio competitivo.
MÉTODOS: Dieciséis estudiantes universitarios fueron aleatoriamente divididos en 2 grupos: placebo (P) y creatina (el he/she CREE). La suplementación se siguió cumpliendo el modelo doble-ciego, 20g de placebo o creatina durante 5 días y después 3 gramos durante 7 días. Antes de la suplementación, los atletas fueron sometidos a la prueba de 1-RM y a la prueba de repeticiones del máximo en el press de pierna 45º (3 juegos de repeticiones del máximo, logró a 80% del valor de 1-RM y separado por 150 segundos de pausa - P 1º set: 9,0 ± 2,4; 2º set: 8,9 ± 2,9 y 3º set: 8,3 ± 3,3 y el CREE 1º set: 10,2 ± 2,2; 2º juego: 9,8 ± 2,9 y 3º set: 9,7 ± 3,5 representantes). Después del periodo del suplementación, los modelos lograron la prueba de la raza en la que los mismos fueron bien educados para alcanzar la distancia más grande posible en 20 minutos. Inmediatamente después de la prueba de la carrera, las pruebas de 1-RM y de repeticiones del máximo se cumplieron otra vez. RESULTADOS: No se observó diferencia en la acción de la prueba de 1-RM. Tampoco había diferencia en la acción de la prueba de la carrera. Después de la prueba de la carrera, un descenso se observó en el número de las repeticiones máximas en el grupo placebo (Representantes - P: 1º set: 7,6 ± 2,6; 2º set: 4,3 ± 2,9*; p < 0,01 y 3º set: 4,6 ± 2,3*; p < 0,01). Esta reducción no se observó en grupo de uso de creatina (Representantes el CREE: 1º set: 10,9 ± 2,9; 2º set: 9,5 ± 2,7 y 3º set: 9,0 ± 3,0).
CONCLUSIONES: La ejecución del ejercicio de paciencia provocó un a fatiga residual que afectó la capacidad de realización de repeticiones del máximo a 80% del valor de un 1-RM. Se relaciona una de las posibles causas de la fatiga en el ejercicio de fuerza al vaciamiento de las acciones de creatina-fosfato. Probablemente, el volumen más grande de creatina-fosfato, inducido por el suplementación, aceleró la re-síntesis de ATP y lo define como bueno como un substrato de energía adicional para el ejercicio competitivo.
Palabras-clave: Creatina. Suplementación. Ejercicio competitivo. Interferencia.
In the last decades, several studies have investigated the effect of the concurrent training, in which endurance and resistance exercises are performed concurrently at the same training session(1). Once athletes and individuals physically active adopt this training strategy, there is a great interest in relation to the interference the first activity would exert on the subsequent one.
Results obtained in our laboratory demonstrated that the endurance exercise (70% of the O2max for 45 minutes in treadmill) promotes a reduction on the performance of the subsequent maximum repetition test in the leg press at 45º(2). Other studies corroborate our findings that the aerobic exercise affects the subsequent strength and power development when endurance exercise is previously performed(1).
In another study, it was observed that the resistance exercise (six series of maximum repetitions in the leg press at 45º with three series at 60% of the 1-RM value and the other three series at 90% of the 1-RM value) did not interfere on the posterior aerobic power performance(3). These results are also corroborated by other researches that demonstrated that the aerobic power performance does not seem to be influenced by the previous execution of resistance exercises(4-8).
However, the literature presents conflicting results(1). Some researches suggest the non existence of interference from the concurrent training on the aerobic power or strength performance(9-12). However, in a study conducted by Nelson et al.(13), it was demonstrated that the performance of concurrent training hinders the aerobic power development. This controversy may be related to the adaptation level to the concurrent training stimulus. It seems that individuals adapted to the concurrent training undergo less interference in relation to untrained individuals(3,14). Other factors also contribute for the discrepancy of results obtained by researches that analyzed concurrent training such as the exercise protocols used and the organization of their variables (intensity, duration and frequency)(15).
Currently, the most consistent data on the concurrent training indicates that this strategy lessens the power and strength gains when compared to the resistance training alone(4-8,16).
There are two hypotheses to explain this harmful interference from the concurrent training. These hypotheses are related to acute or chronic processes(1,15). The chronic hypothesis consists of the idea that after the concurrent training, the muscle would try to adapt itself to both stimuli. However, this is not possible because the endurance training-induced chronic adaptations are frequently inconsistent with adaptations observed during the resistance training. According to the chronic hypothesis, the combination of these two different stimuli could affect the development of these two physical capacities (aerobic power and strength) due to the fact that both induce to different adaptations(1,4,5).
With relation to the acute hypothesis, it is based on the idea that the former activity would lead to a residual fatigue. This fatigue would hinder the performance of the subsequent activity through alterations on the energetic metabolism (lower substrate availability, acidosis, increase on the ammonia concentration)(1).
Considering that the acute hypothesis is a possible explanation for the interference observed in the concurrent exercise, two studies using carbohydrate supplementation during concurrent exercises were performed(2,3). In both studies, the carbohydrate intake exerted no ergogenic effect(2,3).
These results led us to consider that the availability of other energetic substrate would be the limiting factor for the performance of the subsequent resistance exercise. Considering that the creatine-phosphate contributed significantly for the performance of the high-intensity exercise, the objective of this work was to verify the effect of the creatine supplementation on the performance of the concurrent exercise (endurance exercise performed previously to the 1-RM test and the maximum repetition test performed at 80% of the 1-RM value).
Sixteen female Physical Education students (20.1 ± 1.9 years) from the UniFMU University Center were selected. The practice of strength exercises at least three times a week as well as endurance exercises for at least 30 minutes in alternated or simultaneous days was established as inclusion criterion. Other criterion adopted for the participation in this study was the minimum period of 12 months of previous experience in strength training. The sample selection was performed by means of a questionnaire in which the consumption of other nutritional supplements and controlled substances was evaluated. The experimental protocol was approved by the Ethics Committee in Researches involving human beings (CEPSH) of the Biomedics Sciences Institute University of São Paulo (Nº 051.00). The experiments were conducted according to specific resolution of the National Health Council (Nº 196/96). All individuals were informed in details on the procedures used and agreed in participating voluntarily in the study, signing a term of free and informed consent and privacy protection.
Determination of 1-RM and maximum repetition capacity
After a quick warm up exercise, the 1-RM value was determined through three increasing attempts in the leg press exercise at 45º(17). Later, the percentile value equivalent to 80% of the 1-RM value was calculated for the performance of the three maximum repetition sets with intervals of 150 seconds.
The exercise protocol adopted consisted of 20 minutes running in delimited track field in which the subjects ran the longest distance as possible in 20 minutes. As the exercise maintained a constant step rhythm during the entire activity, the test started with a command voice "ready, go", with chronometer turned on simultaneously. The test ended with a whistle sound.
The participants were divided into two groups randomly selected. The creatine supplementation (or placebo) was conducted according to double-blind design. In the first phase (overload), 20 grams a day of creatine (or placebo) were administered, being divided into four doses, during five days. During the second phase (maintenance), three grams of creatine (or placebo) were administered during seven days. The group placebo was used as control and followed the same experimental conditions as the group supplemented with creatine; however, this group received only carbohydrate (maltodextrin).
Two data collections were performed in distinct days 12 days away from one another. At the beginning of the supplementation protocol, both groups were initially submitted to the 1-RM test and to the maximum repetition test in the leg press at 45º (80% of the 1-RM). Twelve days after supplementation (creatine or placebo), both test (1-RM and maximum repetitions) were once again performed shortly after the 20-minutes running test. Therefore, the objective of this work was to verify the effect of the endurance exercise (running) on the strength development in groups placebo and creatine supplemented for 12 days.
The results were analyzed using the analysis of variance (ANOVA two way) (time factor x supplementation factor) followed by the Tukey test (GraphPAD software). The minimum significance level adopted in the present study was of p < 0.05. The results are expressed as average and standard deviation.
The value obtained in the 1-RM test presented no alteration between groups placebo and creatine, with or without the previous execution of the endurance exercise (table 1). The 1-RM value/body weight ratio also remained unchanged in relation to groups placebo and creatine at the beginning (without the previous execution of the endurance exercise) and at the end of the experiments (with the execution of the endurance exercise) (table 1).
However, in the maximum repetitions test, a decrease on the number of repetitions performed by the group placebo after the performance of the aerobic exercise was observed in the two last sets in relation to the beginning of the experiment (P: 1st set: 9.0 ± 2.4; 2nd set: 8.9 ± 2.9 and 3rd set: 8.3 ± 3.3 vs. P: 1st set: 7.6 ± 2.6; 2nd set: 4.3 ± 2.9*; p < 0.01 and 3rd set: 4.6 ± 2.3*; p < 0.01) (table 2). This response was not observed in the group creatine (CRE: 1st set: 10.2 ± 2.2, 2nd set: 9.8 ± 2.9 and 3rd set: 9.7 ± 3.5 vs. CRE: 1st set: 10.9 ± 2.9; 2nd set: 9.5 ± 2.7 and 3rd set: 9.0 ± 3.0). At the end of the experiment, after the endurance exercise, the average number of maximum repetitions performed by the group creatine in the last two sets was higher than that of the group placebo (table 2).
Regarding the 20-minutes running test, no difference was observed on the performance of both groups (table 3).
The objective of the present study was to test the effect of the creatine supplementation on the adverse effect of the endurance exercise on the subsequent strength development. As previously observed, the performance of the endurance exercise affected the subsequent strength development(1,2).
This impairment could be explained due to the performance of the resistance exercise in adverse metabolic and energetic condition, in case this exercise is preceded by an endurance exercise(1). This would occur during strength training performed at the same session and hence characterizing an acute effect. In this context, the muscle would have reduced capacity of developing tension during the performance of the posterior strength training. The possible explanation for this phenomenon was called as acute hypothesis(1,15).
The acute interference hypothesis is supported by the study of Craig et al.(6) who verified that the strength development in the lower limbs was impaired due to the performance of an aerobic exercise shortly before the resistance training. In the same study, it was observed that the lower limbs adaptation was not impaired by the previous endurance training. According to the authors, the legs musculature would not recover from the endurance training and would not perform the resistance training at intensity required to promote the desired adaptations.
The mechanisms responsible for the strength and power impairing in the concurrent training are not yet fully identified(1,15,18,19). A possible candidate is the muscular glycogen depletion, once it is an important energetic substrate for the resistance training(20-22). However, previous evidences obtained in our laboratory demonstrate that individuals who ingested adequate amounts of carbohydrate before exercise and who were also supplemented with carbohydrate during concurrent exercise could not achieve lessening the harmful effect of the endurance exercise on the posterior resistance exercise(2). These results, therefore, made us consider the hypothesis that other energetic substrate could contribute for the performance of the concurrent training (aerobic training performed before the resistance training), the creatine-phosphate (CP).
In the present study, the results observed demonstrate that the strategy of ingesting creatine or placebo did not affect the result of the 1-RM test. Recently, in another study conducted in our laboratory(23), we could verify that the creatine supplementation did not change the maximum load supported in supine, checked through the 1-RM test. However, still in this study, we verified that the creatine intake increased the capacity of performing maximum repetitions at 70% of the 1-RM value. These results are in agreement with results found by other researchers(17,24). Earnest et al.(17) did not observe increases on the 1-RM values for supine after creatine supplementation either. As in our study, Earnest et al.(17) only verified increases on the capacity of performing maximum repetitions at 70% of the 1-RM value.
Despite the energy absolute production during short-duration maximal effort (~6-8 seconds), as in the case of the 1-RM test, be predominantly supplied by the creatine-phosphate degradation, the intramuscular creatine basal content before supplementation would be able to supply this demand. Consequently, the group submitted to creatine supplementation would not present a better performance in relation to the group placebo(17,23,25).
In relation to the capacity of performing maximum repetitions (80%-1-RM) in the group placebo, the endurance exercise promoted a decrease on this parameter in relation to the initial test, as expected. A possible explanation for the reduction on the number of maximum repetitions is that the endurance exercise would promote a depletion of the energetic substrates, thus generating a residual fatigue (acute interference hypothesis)(1,15). On the other hand, in the group supplemented with creatine, the capacity of performing maximum repetitions (80%-1-RM) in the leg press at 45º after endurance exercise was maintained.
The fatigue installation in the resistance exercise seems to be multifactorial, presenting as potential causes the CP depletion, the intramuscular acidosis (increase of H+ ions) and/or the reduction on the muscular glycogen(25). MacDougall et al.(26) observed that the combination between the CP depletion (62% in relation to the rest situation) and the muscular acidosis (21.3 mmol.kg-1 of wet weight) was the responsible for the fatigue at the 1st series of maximum repetition at 80% of the 1-RM value. These authors also reported that after three series of maximum repetitions at 80% of the 1-RM value, the incapacity of maintaining the movement pattern seems to be limited by the increase on the H+ ions concentration(26). The elaboration of this hypothesis was based on the fact that the CP reduction degree (50% in relation to the rest situation) was smaller than that observed in the 1st series (62% in relation to the rest situation). Reinforcing this hypothesis, the lactate production had been higher at the end of the last series (1st series 21.3 mmol.kg-1 wet weight vs 3 series 27.4 mmol.kg-1 wet weight)(26).
Considering that the fatigue at the 1st series may be related with the CP reduction, one may speculate that the higher content of this substrate in the muscle would minimize the CP depletion in the group supplemented with creatine, thus favoring its subsequent re-synthesis for the next series. Furthermore, it is important mentioning the buffering capacity exerted by the ATP-CP system(27). The immediate re-phosphorylation of ADT into ATP through the CP hydrolysis requires one H+ ion(27). As result, this buffering capacity would lessen the harmful effects of the acidosis(25,27,28), such as the inhibition of enzymes involved in the energetic metabolism(25,27) and the reduction on the sensitiveness of the contractile proteins to ions Ca++(28).
Therefore, the increase on the availability of this substrate and its buffering capacity would be responsible for the maintenance of the performance in the subsequent maximum repetitions test in the group submitted to creatine supplementation.
According to other results available in literature, the present study demonstrated that the previous performance of endurance exercises affects the subsequent resistance exercise. It was also verified in this study, that the creatine supplementation is able to nullify the adverse effect induced by the endurance exercises on the subsequent performance on the maximum repetitions test at 80% of the 1-RM value. These results suggest that the ATP-CP system contributes significantly for the performance of the concurrent exercise in which the subsequent resistance training is performed at high intensity.
The authors would like to thank the support of Prof. Flávio Delmanto, Coordinator of the Department of Health Sciences-UniFMU University Center, to MS Prof. Vagner Raso for the aid in the statistical analysis and to PhD Prof. Reury Frank P. Bacurau for the aid in the results discussion.
1. Leveritt M, Abernethy PJ, Barry BK, Logan PA. Concurrent strength and endurance training. a review. Sports Med 1999; 28:413-27. [ Links ]
2. Aoki MS, Pontes Jr FL, Navarro F, Uchida MC, Bacurau RFP. Suplementação de carboidrato não reverte o efeito deletério do exercício de endurance sobre o subseqüente desempenho de força. Rev Bras Med Esporte 2003, 9:282-7. [ Links ]
3. Gomes RV, Matsudo SMM, Almeida VCS, Aoki MS. Suplementação de carboidrato associado ao exercício de força não afeta o desempenho do subseqüente teste de potência aeróbica. Rev Bras Ciên Mov 2003; 11:67-72. [ Links ]
4. Hickson RC. Interference of strength development by simultaneously training for strength and endurance. Eur J Appl Physiol 1980; 45: 255-63. [ Links ]
5. Kraemer WJ, Patton JF, Gordon SE, Harman EA, Deschenes MR, Reynolds K, et al. Compatibility of high-intensity strength and endurance training on hormonal and skeletal muscle adaptation. J Appl Physiol 1995; 78: 976-89. [ Links ]
6. Craig BW, Lucas J, Pohlman R, Stelling H. The effects of running, weightlifting and a combination of both on growth hormone release. J Appl Sport Sci Res 1991; 5:198-203. [ Links ]
7. Dudley GA, Djamil R. Incompatibility of endurance and strength training modes of exercise. J Appl Physiol 1985; 59:1446-51. [ Links ]
8. Hennessey LC, Watson AWS. The interference effects of training for strength and endurance simultaneously. J Strength Cond Res 1994; 8: 12-9. [ Links ]
9. Maccarthy JP, Pozniak MA, Agre JC. Neuromuscular adaptations to concurrent strength and endurance training. Med Sci Sports Exerc 2002; 34: 511-9. [ Links ]
10. Sale DG, MacDougall JD, Jacobs I, Garner S. Interaction between concurrent strength and endurance training. J Appl Physiol 1990; 68: 260-70. [ Links ]
11. Bell GL. Physiological adaptations to concurrent endurance training and low velocity resistance training. Int J Sports Med 1991; 12:384-90. [ Links ]
12. Abernethy PJ, Quingley BM. Concurrent strength and endurance training of the elbow extensor. J Strength Cond Res 1993; 7:233-40. [ Links ]
13. Nelson AG, Arnall DA, Loy SF, Silvester LJ, Conlee RK. Consequences of combining strength and endurance training regimens. Phys Ther 1990; 70: 287-94. [ Links ]
14. Baker D. The effects of an in-season of concurrent training on the maintenance of maximal strength and power in professional and college-aged rugby league football players. J Strength Cond Res 2001; 15:172-7. [ Links ]
15. Docherty D, Sporer B. A proposed model for examining the interference phenomenon between concurrent aerobic and strength training. Sports Med 2000; 30:385-94. [ Links ]
16. Hunter G, Derament R, Miller D. Development of strength and maximal oxygen uptake during simultaneous training for strength and endurance. J Sports Med Phys Fitness 1987;27:269-75. [ Links ]
17. Earnest CP, Snell PG, Rodriguez R, Almada AL, Mitchell TL. Effects of creatine monohydrate ingestion on anaerobic power indices, muscular strength and body composition. Acta Physiol Scand 1995;153:207-9. [ Links ]
18. Dudley GA, Fleck SJ. Strength and endurance training: are they mutually exclusive? Sport Med 1987; 4:79-85. [ Links ]
19. Chromiak JA, Mulvaney DR. A review: The effects of combined strength and endurance training on strength development. J Appl Sport Sci Res 1990; 4:55-60. [ Links ]
20. MacDougall JD, Ray S, McCartney N, Sale DG, Lee P, Garner S. Substrate utilization during weightlifting. Med Sci Sports Exerc 1988; 20:S66. [ Links ]
21. Tesch PA, Colliander EB, Kaiser P. Muscle metabolism during heavy resistance exercise. Eur J Appl Physiol 1986; 55:362-6. [ Links ]
22. Conley MS, Stone M. Carbohydrate ingestion/supplementation for resistance exercise and training. Sports Med 1996; 21:7-17. [ Links ]
23. Aoki MS. Suplementação de creatina e treinamento de força: efeito do tempo de recuperação entre as séries. Rev Bras Ciên Mov 2004; 12:39-44. [ Links ]
24. Volek JS, Kraemer WJ, Bush JA. Creatine supplementation enhances muscular performance during high-intensity resistance exercise. J Am Diet Assoc 1997; 97: 765-70. [ Links ]
25. Lambert CP, Flynn, MG. Fatigue during high intensity intermittent exercise. Application to body building. Sports Med 2002; 32:511-22. [ Links ]
26. Mac Dougall JD, Ray S, Sale DG. Muscle substrate utilization and lactate production during weightlifting. Can J Appl Physiol 1999; 76:1654-60. [ Links ]
27. Mesa JLM, Ruiz JR, Gonzalez-Gross M, Sainz AG, Garzon SJC. Oral creatine supplementation and skeletal muscle metabolism during physical activity. Sports Med 2002; 32: 903-44. [ Links ]
28. Chin ER, Allen DG. The contribution of pH-dependent mechanisms to fatigue at different intensities in mammalian single muscle fibres. J Physiol 1998; 512: 831-40. [ Links ]
Prof. Dr. Marcelo Saldanha Aoki
Prédio 20 Faculdade de Educação Física - UniFMU, Laboratório de Fisiologia do Exercício
Rua Galvão Bueno, 707
01506-000 - São Paulo, SP
Received in 12/10/04. 2nd version received in 5/1/05. Approved in 4/3/05.
All the authors declared there is not any potential conflict of interests regarding this article.