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Print version ISSN 1517-8692On-line version ISSN 1806-9940
Rev Bras Med Esporte vol.12 no.2 Niterói Mar./Apr. 2006
La educación física durante la recuperación nutritiva no afecta el metabolismo muscular de la glucosa en ratones
Vanessa Santhiago; Adelino Sanchez Ramos da Silva; Claudio Alexandre Gobatto; Maria Alice Rostom de Mello
Paulista State University Júlio de Mesquita Filho Rio Claro/SP
This study had the main aim to assess the food intake, the weight gain, and the muscular glucose metabolism in rats submitted to aerobic training while recovering of protean malnutrition. For this, 60 male Wistar rats were separated in two groups: normoprotean (NP) and hypoprotean (HP), according to the diet: NP (17% protein), or HP (6% protein), respectively, which was received after the ablactation (21 days) on their 90th day of life. Then, very animal received the NP diet, and they were submitted or not (trained TRA; sedentary SED) to physical training that consisted in running on a treadmill for 25 m/min., 50 min/day for 5 days a week for 30 days, compounding the NP-SED, NP-TRA, HP/NP-SED, and HP/NP-TRA groups. The glucose metabolism was assessed in slices of the soleum muscle incubated in the presence of insulin (100 µU/L), and glucose (5.5 mM containing glucose [C14], and 2-deoxyglycose [H3]). The daily food intake (g/100 g of the body weight) for the HP/NP-TRA group (24.39 ± 4.07) was larger than the HP/NP-SED group (21.62 ± 4.69). The weight gain (g) was similar in both groups HP/NP-TRA (203.80 ± 34.03) and HP/NP-SED (214.43 ± 30.54). There was no difference between both groups in relation to the following parameters: glucose uptake and oxidation, and glycogen synthesis by the soleum muscle. Thus, it can be concluded that the aerobic training did not have any impact on the nutritional recovery, as there was no metabolic or somatic differences among recovered animals in the presence or absence of the training.
Keywords: Training. Protean malnutrition. Nutritional recovery.
El estudio presente buscó evaluar la ingestión alimentaría, y la ganancia de peso y el metabolismo muscular de la glucosa en ratones sometidos al entrenamiento aeróbico durante la recuperación protéica de la desnutrición. Para esto, 60 ratones del linaje Wistar, los varones estaban separados en grupos: normoprotéico (NP) e hipoprotéico (HP), de acuerdo con la dieta NP (17% de proteína) o HP (6% de proteína), respectivamente, recibida del desmame (21 días) a los 90 días de edad. Todos los animales pasaron entonces, para recibir la dieta NP y algunos fueron sometidos (entrenado TRE) o ningún (sedentario SED) educación física que consistió en carrera de cinta rodante, 25m/min., 50 minutos por día, 5 días en la semana, durante 30 días, que componen los grupos NP-SED, NP-TRE, HP/NP-SED y HP/NP-TRE. El metabolismo de la glucosa se evaluó en las muestras de músculo sóleo incubadas en presencia de insulina (100mU/L) y glucosa (5,5 mM, conteniendo [C14] la glucosa y [H3] 2-deoxiglicose). Mientras que la ingestión diariamente alimentaría (el g/100g de peso corpóreo) del grupo HP/NP-TRE de grupo (24,39 ± 4,07) era más grande que del grupo HP/NP-SED de grupo (21,62 ± 4,69). Las ganancias de peso (g) fueron similares en los grupos HP/NP-TRE (203,80 ± 34,03) y HP/NP-SED (214,43 ± 30,54). No había diferencia entre estos dos grupos en cuanto a los parámetros: la recepción de glucosa, oxidación de glucosa y síntesis del glucógeno para el músculo sóleo. De esta manera, nosotros podríamos concluir que los entrenamientos aeróbicos no tenían el impacto sobre la recuperación nutritiva, porque no había diferencias metabólicas o somáticas entre animales recuperados en presencia o ausencia del entrenamiento.
Palabras-clave: Entrenamiento. Desnutrición portéica. Recuperación nutritiva.
Despite several studies have demonstrated that it is possible to produce sufficient food for the whole world population, 826 million people continue suffering chronic malnutrition. Every year, 36 million people die as consequence of the starvation in the world(1).
The protean malnutrition implies in several metabolic changes. Several studies on underfed children indicate a decrease in the glycemic levels and low hepatic glycogen content(2). The reestablishment of the metabolic variables during the nutritional recovery is slow. According to the literature, the physical exercise can achieve benefits to the nutritional recovery process. Upon the comparison of the growth rhythm in children recovering from malnutrition in hospitals, it was verified that the mild active children who participated in activities involving low energetic expenditure presented more slim mass and had a faster growth than those performing the hospitals' standard procedure(3). Furthermore, it is widely known that the physical training causes an increase in the tissular glycogen reserves(4), and this can help to re-establish the underfed glycidic metabolism.
For obvious reasons, a great number of researches involving the protean malnutrition have been conducted in lab animals, especially rats, once they present metabolic similarities to the human beings. An experimental model of early chronic malnutrition that is highly accepted and which, in many aspects is similar to what happens among the Brazilian childish population is the food protean restriction(5). Nevertheless, as to the importance of the problem, there are few studies in the literature involving biochemical factors in underfed rats associated to the chronic physical exercise.
Gobatto et al.(4) performed a three steps study the metabolic aspects in rats: protean restriction for 60 days followed by a 6 weeks nutritional recovery, and the subsequent physical training constituted by 1 hour of swimming sessions a day five days a week for seven weeks. The authors verified that animals treated with the hypoprotean diet presented similar performance to the rats that were not submitted to the malnutrition, and the tissular glycogen stocks together with the blood lactate response to the acute exercise, as well as the seric substrate concentrations presented similar values between trained groups, regardless their previous nutritional state.
Galdino et al.(2) assessed the effects of the intra-uterine and postnatal protean malnutrition up to the adult age (10 days) in the adaptations of the muscular glucose metabolism on the swimming training. The authors verified an increase in the glucose uptake and in the content of muscular glycogen in trained animals. The glycogen synthesis has been reduced in sedentary underfed animals, but it was restored in the exercised animals. The authors have concluded that the prolonged protean malnutrition did not damage the metabolic adaptations on training.
Papoti et al.(6) assessed the kinetics of the blood lactate in adult rats recovered from protean malnutrition during a swimming exercise, through the determination of the maximal steady lactate phase. For this, each animal performed four swimming tests for 20 minutes bearing overloads between 4.5 and 7.5% of their body weight. The maximal steady lactate phase was attained in the 5.5% load of the body weight at the 5.5 and 4.7 nmol/I blood concentration, respectively, to the control and recovered of underfeeding rats. These results led the authors to conclude that the early malnutrition can affect the lactate kinetics during the exercises.
As there are reports suggesting that the physical training can be benefic to the nutritional recovery process, the present study was planned to assess the muscular glycidic metabolism in rats submitted to early food protean restriction and later nutritional recovery associated or not to the physical training.
MATERIALS AND METHODS
Animals and diets
The experimental sampling was composed by 60 male Wistar rats which initiated the trial in their 21st day of life. All rats were kept in individual polyethylene cages under controlled clare-obscure cycle conditions (12/12 h), with free access to water and food (normoprotean and hypoprotean diets) manipulated at the Laboratory of Biodynamics of the Paulista Júlio de Mesquita Filho State University, Rio Claro campus. The diet composition is described on table 1. Every trial with animals was performed according to the guidelines provided by the European Convention for Protection of Vertebrate Animals used for Experimental and other Scientific Purposes(7).
Planning and experimental groups
The study was composed of two steps. In the first step, the animals were separated in two groups according to the diet: normoprotean fed on a normoprotean diet from their ablactation in the 60th day of life, and hypoprotean fed on a hypoprotean diet from their ablactation in the 60th of life.
Next, it started the second step, when the animals from both groups received a normoprotean portion, thus compounding 4 groups according to their nutritional state and the presence or absence of the physical training: Sedentary Normoprotean (NP-SED), Trained Normoprotean (NP-TRA), Sedentary Hypo/Normoprotean (HP/NP-SED), and Trained Hypo/Normoprotean (HP/NP-TRA). In case of animals initially submitted to the protean malnutrition, the normoprotean portion nutrition had as purpose the nutritional recovery.
During the whole experiment, the animals had their weight gain and amount of food ingestion recorded once a week. Also, they had their muzzle-anus length measured at the end of the first and second steps.
Physical training protocol
Before beginning the training period, the rats assigned to the NP-TRA and HP/NP-TRA groups performed a 10 days adaptation on the treadmill, where they ran for 5 minutes at a 10 m/min velocity for 3 days, 6 minutes at the 15 m/min velocity for 3 days, and 7 minutes at a 20 m/min velocity for 3 days.
After the adaptation, the training period initiated, and it was composed by daily running sessions 5 days a week for 4 weeks. The time of the exercising and the treadmill velocity were gradually incremented up to the animals ran for 50 minutes at a 25 m/min velocity. Such training protocol was chosen because these are aerobic exercises prescribed for rats(8).
Before and after the training period, the NP-TRA and HP/NP-TRA groups were submitted to a running session on the treadmill at constant 25 m/min velocity for 20 minutes. On rest and every 5 minutes running, every rat was withdrawn from the treadmill for a few minutes, to collect 25 µl blood from the tail in order to determine the lactate. The blood samplings were immediately transferred to Eppendorf tubes with 50 µl hemolyzing solution (1% sodium fluoride) for later blood analysis in an electrochemical analyzer (YSI 1500 Sport Lactate Analyzer).
Sacrifice of animals and obtention of biological material
48 hours after the last strength test, the animals were sacrificed on rest through decapitation. The trunk's blood was collected in glass tubes, where it was separated from the serum, in order to determine the amount of glucose, free fatty acids (FFA), albumin, and protein. After the blood collection through a median laparotomy, a portion of the liver was withdrawn for later determination of the glycogen and fat. The two soleum muscles were extracted from the back foot for the incubation procedures, aiming to assess the glycidic metabolism. 48 hours after the first strength test, the blood sampling was collected from the cut located on the distal end of the animals' tail, in order to determine the glucose concentrations, the FFA, the amount of protein and the albumin(9).
Incubation of the soleum muscle
The slices of the soleum muscle were placed in siliconized 20 mL scintillation vials containing 1.5 mL of Krebs-Ringer bicarbonate buffer.
The vials were closed with rubber lids, sealed with plastic rings and submitted to 30 minute pre-incubation under agitation in Dubinoff bath at 60 rpm and continuing gas injection with O2/CO2 (95%/5%). After that period, the muscle slices were transferred to new scintillation vials (external vial), and inside the vial it was installed small shell-shaped tubes (internal vial) with an approximately 3 cm straight stick inserted through the rubber lid of the external vial. Each external vial contained 1.5 mL of the Krebs-Ringer buffer, and each internal vial had 700 mL of 10x hyamine. After 60 minute incubation in that system with gas injection for the first 15 minutes, it was added 100 µL of 25% trichloroacetic acid (TCA) in the external vial, with the purpose to release the CO2. The preparation was kept in that system for more than three hours. After that period, 200 µL of the liquid contained within the internal vial was withdrawn, in order to determine the produced CO2. The acidified incubation media contained in the external vial was stored to determine the lactate, and the muscle slice was immediately digested in 0.5 mL KOH, in order to assess the muscular glycogen content(11). The pre-incubation and incubation temperature was 37º.
The Krebs-Ringer buffer, which was the basis to the pre-incubation and incubation medias consisted in 6% NaCl, 6.64 mM HEPES, 0.032% KCL, 1.12 nM CaCL2, 0.015% KH2PO4, 0.19% NaCO3, 0.03% MgSO4. That solution was gas injected for 30 minutes in O2/CO2 (95%/5%), and the pH was adjusted at 7.4. It was added 20 volumes of fat free seric albumin to that solution, and sodium pyruvate was added to the pre-incubation media in the 5 mM concentration, and (5.5 mM) glucose was added to the incubation media containing (0.25 µCi/mL) glucose [U-14C], (2-DG = 0.5 µCi/mL) 2-deoxyglucose [3H], and (10025 µUL/mL) insulin.
After these additions, the pH was adjusted at 7.4, and the media was transferred to the vials that were sealed and balanced into a 37ºC bath under O2/CO2 gas injection for at least 15 minutes. Slices of the same muscle of similar weight than the incubated ones were used to determine the controlling glycogen concentration. It was assessed the glucose uptake using the 2-DG as marker, incorporating the 14C to the glycogen (synthesis), and measuring the radioactivity of the glycogen's 14C and the 2-DG's 3H contained respectively in the precipitate and in the alcoholic phase of the glycogen extraction using the counter of the beta particles. The lactate released into the incubation media was determined separating the metabolites in the ionic exchange column (Dowex-2, Sigma), and this represents an index of the glucose transportation under these conditions. Besides the radioactive lactate, the total lactate was also determined into the incubation media using the enzymatic method. In order to assess the oxidant glucose (CO2 production), the 14C radioactivity present in the liquid (hyamine) collected in the internal vial of the incubation system was determined.
The seric glucose was determined using the enzymatic method of the oxidase glucose, the total proteins was determined by the biuret reagent, the albumin was determined by the bromokresol green, and the FFA was determined by the carbamate diethyl. The tissular glycogen was extracted according to the Sjorgren et al.(10) method, and the color of the precipitate was performed using the phenol, following Dubois et al.(11).
The statistical procedures included the unpaired t Student or the two-entry ANOVA test, and whenever necessary, this was followed by the post-hoc Bonferroni test. In every case, it was adopted p < 0.05 as significance level.
In the first phase of the study, the animals that received the hypoprotean diet had a significantly lower weight gain, and a significantly higher food intake compared to the normoprotean groups after the 8 week training period with the diets (table 2). The animals kept in the hypoprotean diet presented significantly higher seric FFA concentrations, and significantly lower total proteins, albumin, and glucose related to the normoprotean group (table 2).
In the second step of the study, the trained groups (NP-TRA, and HP/NP-TRA) showed a daily food intake higher than the respective sedentary groups (NP-SED, and HP/NP-SED) (table 2). Related to the weight gain, the trained recovered groups (HP/NP-TRA) had a significantly lower gain compared to the remaining groups (HP/NP-SED, NP-TRA, and NP-SED).
Figure 1 shows the blood lactate values of the trained groups (NP-TRA, and HP/NP-TRA) on the strength tests performed before (upper panel) and after (lower panel) the physical training. In the test performed before the training, the blood lactate was stabilized from 5 minutes of the exercise in the recovered group (HP/NP-TRA), and after 10 minutes in the control group (NP-TRA) with similar values (3.72 ± 1.06, and 4.60 ± 1.44 mmol/L to the NP-TRA, and the HP/NP-TRA, respectively).
In the test performed after the 4 week training period, the blood lactate stabilized after 5 minutes of the exercise in both groups, presenting lower values than those observed in the first test (2.98 ± 1.02, and 2.15 ± 0.97 mmol/L, to the HP-TRA and HP/NP-TRA, respectively). The reduction was significant only to the recovered group (HP/NP-TRA).
The trained control group (NP-TRA) presented a significantly higher hepatic glycogen concentration compared to the remaining groups (NP-SED, HP/NP-SED, and NP-TRA). As to the hepatic lipids, it was verified no significant differences between groups. The muscular glycogen presented significant lower values in trained animals (NP-TRA, and HP/NP-TRA) than the sedentary ones (NP-SED, and HP/NP-SED (table 3).
There was no significant difference between the values of the glucose uptake, the glucose oxidation and incorporation (synthesis) in the glycogen by the isolate soleum muscle in rats (table 3).
The results related to the gain in the body weight, food intake and the length in rats in the first step of the study indicated that the 6% protein diet was effective in inducing a malnutrition state in animals. Likewise, the blood glucose concentrations, the total protein, low albumin and high seric FFA content are commonly observed features in underfed human beings(12) and in underfed animal models(2,13,14).
The reduction in the body weight gain in rats submitted to protean restriction is a widely reported phenomenon in the literature(15), and this has been associated to functional and morphological changes.
Rats kept in a hypoprotean diet showed a similar picture than the childish Kwashiorkor-type malnutrition that has as basic characteristics: low weight, hypoproteinia, hypoalbuminemia, high seric FFA contents and hepatic steatosis or fatty liver(1,2).
An important aspect is that the alterations observed were consequence of the protean instead of the caloric deficiency, as both were isocaloric diets and the underfed rats of such model presented a higher food intake than the control group. Several prior studies within our group have shown a high food intake in rats kept in a hypoprotean diet(4,8,16).
Among other factors, such alteration may be due to modifications in the mediators such as the ghrelin and the leptin. Leptin is a protein produced by the adipose tissue that has an important role through the increase in the calories burning and in the reduction in the food intake(17-19). Ghrelin is a gastrointestinal hormone recently identified in the rats' stomach(20). Studies in animal models indicate that the ghrelin has an important role in the signalization of the hypothalamic centers that regulate the food intake and the energetic balance(21-25).
Still, it is known that the seric ghrelin concentrations are influenced by the acute and chronic changes in the nutritional state, and they rise in the presence of nervous anorexia, and reduced in the obesity, unlike the leptin that is high in obese rats(24,26-28). Thus, it is necessary to assess the protean malnutrition model, in order to precisely interfere in the contribution of those mediators as to the food intake alterations observed in the present study.
In the second step of the study, it was verified that the amounts of seric protein, glucose, albumin, and FFA, as well as the hepatic and muscular glycogen and the hepatic lipids presented similar concentrations in the recovered animals than the amounts found in the control animals. The body mass remained reduced. These data are in accordance to the previously found results(1,6), and they suggest the partial nutritional recovery in previously underfed rats.
The glucose uptake, oxidation, and incorporation (synthesis) in glycogen by the isolate soleum muscle in rats at the end of the second phase of the study present no significant differences between the recovered and control groups both in trained and sedentary animals, suggesting that the early food protean restriction does not interfere in the muscular metabolism of the glucose after the recovery.
Crace et al.(29) reported an increase in the glycogen synthesis in rats submitted to protean restriction, and also a higher glucose uptake by the isolate soleum muscle in rats submitted to hypoprotean diet compared to rats fed with a normoprotean diet. On the other hand, Nolte et al.(30) found no difference in the glycogen synthesis between the control and protein recovered animals, and they studied muscular slices incubated upon the absence and presence of FFA.
The results in the strength tests showed that the training treadmill running protocol led to a reduction in the blood lactate accumulation during the exercise, suggesting an improvement in the aerobic conditioning in these animals. Normally, the physical training reduces the lactate accumulation during the exercising at the same submaximal intensity of the strength due to a higher removal capability of the circulating substrates(31).
On the other hand, the reduction in the blood lactate during the exercises in the trained groups compared to the sedentary groups may be due simply to the reduction in the muscular reserves of the glycogen, since in the present study the animals submitted to the physical training, regardless their nutritional state, presented a reduction in the muscular glycogen concentrations. These results are opposed to the classical reports found in the literature, indicating the presence of high muscular glycogen stocks in human beings and animals submitted to the physical training(32).
Considering that trained and sedentary animals have shown similar values for the glucose uptake and in vitro glycogen synthesis by the isolate soleum muscle, the low muscular glycogen concentrations in the trained groups assessed in the resting state may be consequence of the incomplete recovery after each exercising session.
Summarizing, it can be concluded that the hypoprotean diet in the first step was efficient in inducing the protean malnutrition state in rats. In the second step, there was a return of the biochemical parameters values analyzed in the normal state.
As to the training, it had no impact on the nutritional recovery, as there were no metabolic or somatic changes between the recovered animals in the presence or absence of the training. It was detected only an increase in the food intake among the trained groups, suggesting a reduction in the alimentary efficiency, in function of the increasing energetic demand that is consequence of the training.
Probably, the frequency of the exercise was inadequate to observe the desired effects. Further studies are in progress in our laboratories aiming to enlarge the animals' training period, as well as to assess the oxidant adaptation of the skeletal muscle through the enzymatic activities.
All the authors declared there is not any potential conflict of interests regarding this article.
1. Torun B, Chew F. Protein energy malnutrition. Modern nutrition in healthy and disease. Philadelphia: Lea e Febiger, 1994. [ Links ]
2. Galdino R, Mello MAR, Almeida RL, Almeida CCS. Desnutrição protéico-calórica. In: Dâmaso A, editor. Nutrição e exercício na prevenção de doenças. São Paulo: Medsi, 2001;10:433. [ Links ]
3. Torun B, Viteri FE. Influence or exercise on linear growth. Eur J Clin Nutrition 1994;48(Suppl I):S186-90. [ Links ]
4. Gobatto CA. Alterações metabólicas decorrentes do treinamento físico em ratos previamente desnutridos e recuperados. [Dissertação de Mestrado]. UNICAMP, Campinas, 1993. [ Links ]
5. Gopalan S. Malnutrition: causes, consequences, and solutions. Nutrition 2000; 16:7-8. [ Links ]
6. Papoti M, Almeida PBL, Prada FJA, Eleno TG, Hermini HA, Gobatto CA, et al. Máxima fase estável de lactato durante a natação em ratos recuperados de desnutrição protéica. Motriz, Rio Claro 2003;9:97-103. [ Links ]
7. Concil of Europe. Strasbourg, no. 123, 1985. [ Links ]
8. Manchado FB, Gobatto CA, Contarteze RVL, Papoti M, Mello MAR. Maximal lactate steady state in running rats. J Exerc Physiol online 2005;8:1-8. [ Links ]
9. Nogueira DM. Métodos de bioquímica clínica. São Paulo: Pancast, 1990;468p. [ Links ]
10. Sjorgren B, Nordensklold T, Holmegren H, Wollerstrom J. Beitrag zur kenttnis des lebernrhytmik. Pflugers Archiv fur die Gesamte Physiologie des Menschem und der Tiere 1938;240-7. [ Links ]
11. Dubois B, Gilles KA, Hamilton JK, Rebers PA. Colorometric method for determination of sugar relate substances. Analytical Chemistry 1956;28:350-6. [ Links ]
12. Waterlow JC, Alleyne GAO. Má nutrição protéica em crianças. Evolução dos conhecimentos nos últimos dez anos. São Paulo: L.P.M. Editora, 1974;120p. [ Links ]
13. Lunn PGE, Austin S. Differences in nitrogen metabolism between protein-deficient and energy-deficient rats with similary restricted growth rates. Ann Nutr Metab 1983;27:242-51. [ Links ]
14. Gobatto CA, Sibuya CY, Azevedo JRM, Luciano E, Kokubun E, Mello MAR. Caracterização da intensidade de exercício e do efeito de treinamento físico no modelo de natação de ratos Wistar. Motriz, Rio Claro 2001a;7:S57-S62. [ Links ]
15. Escriva F, Kergoat M, Bailbé D, Pascual-Leone AM, Portha B. Increased insulin action in the rat after protein malnutrition early in life. Diabetologia 1991;34:559-64. [ Links ]
16. Carneiro EM, Mello MAR, Gobatto CA, Boschero AC. Low protein diet impairs glucose-induced insulin secretion from and 45Ca uptake by pancreatic rats islets. Nutr Biochem 1995;6:314-8. [ Links ]
17. Romero CEM, Zanesco A. O papel dos hormônios leptina e grelina na gênese da obesidade. Braz J Nutr 2005 (in press). [ Links ]
18. Ahima RS, Osei SY. Leptin signaling. Physiol Behav 2004;81:223-41. [ Links ]
19. Moraes C, Romero CEM, Zanesco A. Serum leptin level in hypertensive middle-aged obese women. The Endocrinologist 2005;15:219-21. [ Links ]
20. Kojima M, Hosoda H, Matsuo, H, Kangawa K. Ghrelin: discovery of the natural endogenous ligand for the growth-hormone secretagogue receptor. Trends Endocrinol Metabol 2001;12:118-22. [ Links ]
21. Hosoda H, Kojima M, Matsuo H, Kangawa K. Ghrelin and des-acyl ghrelin: two major forms of rat ghrelin peptide in gastrointestinal tissue. Biochem Biophys Res Commun 2000;279:909-13. [ Links ]
22. Nakazoto O, Murakami N, Date Y, Kojima M, Matsuo H, Kangawa K, et al. A role for ghrelin in the central regulation of feeding. Nature 2001;409:194-8. [ Links ]
23. Broglio F, Benso A, Castiglioni C, Gottero C, Prodam F, Destefanis S, et al. The endocrine response to ghrelin as a function of gender in humans in young and elderly subjects. J Clin Endocrinol Metab 2003;88:1537-42. [ Links ]
24. Rosická M, Krsek M, Matoulek M, Jarkovská Z, Marek J, Justová V, et al. Serum ghrelin levels in obese patients: the relationship to serum leptin levels and soluble leptin receptors levels. Physiol Res 2003;52:61-6. [ Links ]
25. Vendrell J, Broch M, Vilarrasa N, Molina A, Gómez JM, Gutiérrez C, et al. Resistin, adiponectin, ghrelin, leptin, and proinflammatory cytokines: relationship in obesity. Obes Res 2004;12:962-71. [ Links ]
26. Tshop M, Smiley DL, Heiman ML. Ghrelin induces adiposity in rodents. Nature 2000;407:908-13. [ Links ]
27. Ukkola O, Poykoo S. Ghrelin, growth and obesity. Ann Med 2002;34:102-8. [ Links ]
28. Salbe AD, Tshop MH, Delparigi A, Venti C, Tataranni PA. Negative relationship between fasting plasma ghrelin concentrations and ad libitum food intake. J Clin Endocrinol Metab 2004;89:2951-6. [ Links ]
29. Crace CJ, Swenne I, Khon PG, Strain J, Milner ROG. Protein energy malnutrition induces changes in insulin sensitivity. Diabetes Metab 1995;16:484-91. [ Links ]
30. Nolte LA, Galuska D, Martin IK, Zierath JR, Wallberg-Henriksson H. Elevated free fatty acid levels inhibit glucose phosphorylation in slow-twitch rat skeletal muscle. Acta Physiol Scand 1994;151:51-9. [ Links ]
31. Pyne BD, Lee H, Swanwick KM. Monitoring the lactate threshold in world-ranked swimmers. Med Sci Sports Exerc 2001;33:291-7. [ Links ]
32. Gobatto CA, Mello MA, Sibuya CY, Azevedo JR, Santos LA, Kokubun E. Maximal lactate steady state in rats submitted to swimming exercise. Comp Biochem Physiol A Mol Integr Physiol, New York 2001;130:21-7. [ Links ]
33. Reeves PG, Nielsen FH, Fahey JGC. AIN-93 purified diets for laboratory rodents: final reports of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodents diet. J Nutr 1993;23:1939-51. [ Links ]
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Received in 30/6/05.
Final version received in 3/10/05.
Approved in 4/11/05.