SciELO - Scientific Electronic Library Online

vol.12 issue2Reliability of the four series 15-second vertical jumping testGeographical dimensions of fibers from the soleum muscle in rats exercised on treadmill: the importance of the analysis by means of digitalized images author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Revista Brasileira de Medicina do Esporte

Print version ISSN 1517-8692On-line version ISSN 1806-9940

Rev Bras Med Esporte vol.12 no.2 Niterói Mar./Apr. 2006 



Track test to assess the lactic ability in high level runners


Pruebas de pista para la evaluación de la capacidad láctica de corredores de velocidad de nivel alto



Fernando Roberto de OliveiraI; Adriano Eduardo Lima-SilvaI; Fábio Yuzo NakamuraII; Maria Augusta Peduti Dal' Molin KissIII; Monique da Silva Gevaerd LochI

ILaboratory of Morpho-Functional Researches – Center of Physical Education and Sports – Santa Catarina State University – SC
IIGroup of Study and Research in Metabolism, Nutrition and Exercise – Center of Physical Education and Sports – Londrina State University Parana – PR
IIILaboratory of Sportive Performance – School of Physical Education and Sports – São Paulo University – São Paulo – SP

Correspondence to




The anaerobic ability (AnA) is given by the sum of the alactic and lactic (AlA) ability. The cycloergonometer has been used at the maximal 30-second strength (max30) to the AnA approach (mean power and fatigue index). Presently, the AnA is not directly measured, and it is required an operational approaching, such as the measurement of the performance and the blood lactate concentration ([La]). In high-level velocity runners, it is expected that on long-endurance, that is, 60 seconds (max30) strength there would be a higher AnA demand, and aiming the ecological validity, it must be applied on the track. The purpose of this study was to make a comparison between the derived variables of the (max30) and (max60) in runners. Eight male national and international 19-27 years old athletes were submitted to max30 and max60 running tests on an official synthetic track, where it was performed an arterialized blood collection from the ear lobule immediately 1, 3, 5, 7.5, and 10 minutes after the strength. To make a comparison between the max30 and the max60, it was used the t-Student test and the simple Pearson's correlation to check the association between variables. The lactate concentrations were significantly higher at max60 than at max30 (20.9 ± 1.2 vs. 18.2 ± 1.9 mM, p < 0.05). Oppose to this, the mean velocities were significantly lower (7.9 ± 0.2 vs. 9.1 ± 0.2 m.s-1, respectively). Significant correlations were found between max30 and max60 (r = 0.92; p < 0.05). However, it was not seen the same as to the lactate (r = 0.62; p > 0.05). According to these athletes' characteristics, who are highly tolerant to elevated [La], the derived test variables with duration/distance close to the 60 s/500 m seem to be more adequate as AnA indexes.

Keywords: Anaerobic test. Blood lactate. Bioenergetics.


La capacidad anaerobica (CAn) se da por la suma de la capacidad aláctica y láctica (el CLa). En el cicloergómetro, la prueba de esfuerzo máximo de 30 segundos (max30) se ha usado para el acercamiento de CAn (la potencia média y el índice de fatiga). Ahora, la CLa no es directamente medido, mientras se necesitando el accionar las aproximaciones como son la medida de la actuación y la concentración sanguínea de lactato ([La]). Con corredores esprínteres de nivel alto, se ha esperado que, en el esfuerzo de duración más grande, es decir 60 segundos (el max60), se pase a una demanda más grande del CLa, y que para la validez ecológica, debe aplicarse en la pista. El objetivo del estudio presente fue el de comparar las variables derivadas de max30 y max60 en los esprínteres. Ocho atletas de nivel internacional y nacional (19-27 años) se sometieron a las pruebas de max30 de la carrera máxima y max60, en pista sintética oficial con recolección de sangre arteriolizado del lóbulo aricular, inmediatamente, a los uno, tres, cinco, siete y medio y diez minutos después del esfuerzo. Para la comparación entre el max30 y max60 la prueba de t de Student y la correlación simple de Pearson se usaron para la comprobación de la asociación entre ambas variables. Las concentraciones del lactato eran significativamente más grandes en el max60 que en el max30 (20,9 ± 1,2 contra 18,2 ± 1,9 mm, p < 0,05). Antagónicamente, las velocidades eran significativamente más pequeñas (7,9 ± 0,2 contra 9,1 ± 0,2 m.s1; respectivamente). Se encontraron correlaciones significantes entre las velocidades en el max30 y max60 (r = 0,92; p < 0,05), sin embargo, no pasó lo mismo para el lactato (r = 0,62; p > 0,05). Para las características de estos atletas se define que pose una gran tolerancia a los altos [La], las variables derivadas de pruebas con el duración/distancia íntimo a los 60s/500m parecen más apropiadas para los índices de CLa.

Palabras-clave: Prueba anaeróbica. Lactato sanguíneo. Bioenergética.




The anaerobic ability (AnA) is a determinant factor of the performance in demanding sportive contests of prolonged maintenance to the high power energy supply, and its equation is given by the sum of the phosphagen/alactic and glycolytic/lactic ability. In the assessment process of the AnA, there is no accordance to the "golden patterns" in this measurement, and generally, the physical/mechanical work/power measurements generated from predominantly anaerobic strengths are used with 30 to 120 seconds duration protocols(1-3). In some situations, these strengths are at the same time the lactate concentrations ([La]) are measured(4), together with an assessment of the maximal accumulated Oxygen deficit(5,6). Another increasingly used approach is the derivation of the anaerobic participation from the determination of the critic power(7,8).

The maximal strength 30-seconds tests on an cycloergonometer (max30) – such as the Wingate test – has been used to set the AnA approach in athletes using the mean power and the fatigue index for that purpose(9). From the analysis of several studies, Bar-Or(9) has concluded that the correlations between the indexes of the Wingate test and the anaerobic performance task are high, but not enough to be used as predictor of the success in other specific tasks, justifying the search for more specific methods for the majority of non-cyclist athletes. Furthermore, it is expected that the max30 has insufficient duration to be used as an index of the lactic ability, since it is required a higher duration to that extent.

Withers et al. found that well trained cyclists attained higher [La] values at 60 and 90 s strengths than at 30 s, with the higher Oxygen deficit appearing on the 60th second. Studying 5 to 120 s strengths, Yamamoto and Kanehisa(11) have verified that the [La] increases up to the 60th second, remaining constant at higher durations. Opposite to this statement, studies performed on an ergometric bicycle has shown that despite the trend for an increasing [La] with an increase in the test duration, it was found no significant differences between the [La] at 30, 40, and 60 s strengths (13.0 ± 2.1 to 14.6 ± 1.3 mM) in highly trained road cyclists(2). In accordance to this, Dudek et al.(12) have found similar [La] values between 30 and 45 s strengths (12.7 ± 2.6, and 12.5 ± 4 mM) in non-athlete individuals.

In high level runners, it is expected that on a high-duration strength, that is, 60 s (max60), there is a higher demand for AnA than at max30 strength(13), and aiming the ecologic validity, the test must be applied on a track. A typical example of that approach in the athletic training routine is the application of tests such as the 500 m running(14,15), that in well anaerobic trained athletes has duration between 60 and 75 s. So, despite it is expected high lactate levels at max30 supplied by the higher lactic power in those athletes, it is expected that in strengths such as max60, it can be found higher [La] values. As to this topic, there is a gap in the literature with comparative approaches in high level runners when it is applied the field test. The purpose of this study was to make a comparison between the derived variables of the max30 and max60 in high level runners.



Eight national and international level (23.8 ± 3.3 years) runners with high level performance on 100 m (10.45 s to 10.78 s), 200 m (20.26 s to 21.40 s), and 400 m (45.80 to 48.70 s) running were submitted to max30 and max60 running test on a 400 m official synthetic track. Every individual signed an informed consent term, agreeing to participate in the study, according to the Ethics Committee in Research with Human Beings of the Santa Catarina State University. The test sequence was randomly determined with 24 to 90 hour intervals. Next to each running, it was collected 20 µl of arterialized blood from the ear lobule (previously prepared with Finalgon®) on the exact 1, 3, 5, 7.5 and 10 moment after the strength to measure the [La]. The [La] determination in the total deproteinized blood with perchloride acid was performed through a spectrophotometer (Guilford 300N) using an enzymatic method with NADH extinction, following a description made by Mader et al.(16). The highest individual [La] value found during each after-test collection was used to the analysis, and the maximal [La] in every measurement was found between 3 and 7.5 minutes. The glycolytic power generated along the tests was calculated dividing the blood's [La] by the time of the exercise (mM.s-1), assuming an increasing linear relationship between these variables.

For the comparison of the amounts of the central trend between the max30 and max60, the t-Student test was used for dependent sampling, with application of the simple Pearson's correlation to check the association between results, being considered significant those differences with higher than 95% probability (p < 0.05).



Table 1 shows the results for the variables assessed at max30 and max60. It was impossible for two individuals to perform the max60 test, one for being injured and the other by the residual fatigue derived from prior training sessions to the test, according to report made by his coach.



It was found significant differences between the assessed variables at max30 and max60, with r = 0.92 (p < 0.05), between the velocities and the low correlation between the [La] (r = 0.62; p < 0.05). It was found no significant correlations between the Dmax30 and [La]max30 (r = –0.44; p > 0.05), but they were significant between the Dmax30 and [La]max60 (r = 0.82; p < 0.05) (figure 1 and 2).






The obtainment of evidences to a test validity can be made verifying the logic validity (or face) of the content, criterion (concurrent and predictive) and constructo(17). In the Sciences of the Sports field, the most used is the one in which the result of a test is compared and/or associated to the amounts attained in another one, and the last one is used as main reference criterion. Nevertheless, in several situations the criterion employed may not contain sufficient and/or satisfactory elements to make the desired interpretations from its explanation; this is the case of the assessment of the AnA in runners.

Here, it was assumed the presupposition that at the light of the present knowledge, the specific performance assessment at the lab does not aggregate any value to the tests which are applied in these athletes' day-to-day training.

In the present study, the measurement of the [La] was used as reference criterion. In the analysis, uneven weights were found in the relationships between the [La] and the distances ran with no significant correlations between the Dmax30 and [La] max30 (r = –0.44; p < 0.05), and significant association between the Dmax60 at the [La] max60 (r = –0.82; p < 0.05). This last figure can be fitted in the higher level found by other authors, with competitive performance in 400/800 s running and 30-60 s duration treadmill running (r between –0.67 and –0.89)(18-22). Despite the limitations of the size of the sampling in the present study, the higher value of the association between Dmax60 and the [La] max60 is in accordance to the theoretical model proposed, stating that the max60 is more dependent from the AnA than the max30, and it can be more adequate to deplete it. As to the Dmax30, some variables such as the power and the alactic ability may have a more significant weight in determining the result of this test with less influence in the final Dmax60 results.

Despite these statements, one must be caution when trying to support the [La] validity as being a direct AnA reflexion. The peak [La] after intense exercising is frequently used as a measure to indicate the energy releasing by the lactic(20), but there is much controversy on the meaning and consequences of the lactate production during and after the exercise(23-25).

Some determining factors for this discussion and its consequent implications are associated to the possible [La] dependences on "foreign" factors, such as the lactate diffusion ability(26), blood volume(27,28), and variability of its measurement(19). Furthermore, the [La] cannot reflect the muscular lactate production, and it even does not allow indication of the energy derived by the phosphagenic system. Thus, although the [La] indicate the extension of the use of the anaerobic glycolysis, it cannot be considered the AnA quantitative measure. Besides, the [La] is not recommended as base to compare the AnA in different individuals(6), and it can be mentioned as an indirect method, and probably, as much valid as the usually used tests(4). Consequently, its usage as a criterion is the limitation to the inferences found in this study.

Related to the optimum duration for the LaC in runners, the present paper reports that the max30 is not ideal for such purpose. However, it does not answer if the max30 used in similar durations as the ones used in this study is the best choice to be employed rather than higher of the max60. Here, for the analysis artifice, it will be used the quali-quantitative comparison of the results found and the specific knowledge gathered in the literature.

With two to three minute strengths, the [La] is significantly higher than in the max30(29). Nevertheless, at 60 and 75 s durations, it is found slightly higher amounts (20 to 22 mM), on two to eight minute strengths (17 to 18 mM)(30,31). In 400 m running performed by long-distance runners, there is an increase in the [La], with an increase in the partial distances (100, 200, 300, and 400 m) and higher acceleration of the [La] accumulation rate appearing around the 27th second of running identified from the curve derived from the [La] relationship vs. time(25).

Despite the [La] increment, from that duration, the acceleration rate decreases. That derivation in a running suggests that the maximal lactic power can be found in higher duration strengths than on a cycloergonometer, where this last one can be close to the first 15 s of strength(11,32).

So, the high [La] amounts found at max30 in the present study can be associated to the high glycolytic power these athletes have, that allows to attain higher [La] levels upon lower durations. Despite a probable lower [La] acceleration rate in intermediary distances, at max60, the final values attain higher levels than at max30, since at max60 the glycolysis remained active for more time at high rate of power supply. The difference found in the lactic power used in the tests supports these statements (table 1).

Besides of considering the [La], the option for a test duration of anaerobic variables must consider the contribution of the lactic percentage while performing it. Gollnick and Hermansen(33) have estimated that 60% of the power at max60 running comes from the anaerobic glycolysis. In high level runners, we have a combination of a band with higher Oxygen deficits(10), and fitted in as tolerance lactate activity, that is, 1 to 8 min(13).

Besides the duration, it seems that at max60 the participation of each system varies according to the activity employed, since with the increase in the muscular mass involved, there is the participation of a higher aerobic percentage(14), and an increase in the total power demand, confirming by the findings stating that the higher maximal accumulated Oxygen deficit is found in activities with higher muscular mass involved(6,35).

Although several factors may contribute to the fatigue on 50 and 90 s strengths, it can be presumed that the main determining variable is the ability in tolerating lower intracellular pH levels(13), associated to the maximal accumulated Oxygen deficit, normally found at that level(6). Together, the above information suggests that when running with durations close to the max60, the higher muscular mass involved, the higher the probability to find higher [La] values, and in more prolonged tests, it causes an increase in the percentage of aerobic participation during the test and a progressive decrease in the [La].

One of the problems found in the high intensity and higher duration tests than the ones employed in this study is that the athlete can use a rhythm strategy and does not produce a maximal strength from the beginning of the test, making difficult to interpret the results. Despite the recommendation that the tests should be performed at maximal strength from the beginning, the partial distances ran were not controlled, and this would be a demand for future studies with athletes of similar level. It is recommended to analyze several strategies that influence the partial and global results in this type of assessment.

The comparative approach used in the present study does not allow many inferences on the possible combinations between the lactic/alactic power/ability in the assessed test performance. In high output athletes, the percentual participation of each of these components on the specific skill of runners must be set. Furthermore, the methodological characteristics of athletes' training who were under the supervision of the same coach when the tests were performed, may have driven some of the results.

So, it is necessary to check the possibility to generalize the findings of the present study by applying similar approaches in several training mesocycles on other groups (for instance, female runners).

From the results found in the present study added to the data found in the literature and the characteristics of high leveled runners who have high tolerance to elevate [La] levels, it was attained evidences of the validity that those variables derived in tests with duration/distance close to the 60 s/500 m seem to be more adequate as the LaC index in runners than the 30 s tests.

All the authors declared there is not any potential conflict of interests regarding this article.



1. Bar-Or O, Dotan R, Inbar O. A 30 second all-out ergometric test its reliability and validity for anaerobic capacity. Isr J Sports Sci 1977;13:126.         [ Links ]

2. Craig NP, Pyke FS, Norton KI. Specificity of test duration when assessing the anaerobic lactacid capacity of high-performance track cyclists. Int J Sports Med 1989;10:237-42.         [ Links ]

3. Matsudo VKR. Avaliação da potência anaeróbica teste de corrida de 40 segundos. Rev Bras de Cienc Esporte 1979;1:8-16.         [ Links ]

4. Vandewalle H, Pérès G, Monod H. Standard anaerobic exercise tests. Sports Med 1987;4:268-89.         [ Links ]

5. Green S, Dawson B. Measurement of anaerobic capacities in humans. Definitions, limitations and unsolved problems. Sports Med 1993;15:312-27.         [ Links ]

6. Gastin PB. Quantification of anaerobic capacity. Scand J Sci Sports 1994;4:91-112.         [ Links ]

7. Bulbulian R, Wilcox AR, Darabos BL. Anaerobic contribution to distance running performance of trained cross-country athletes. Med Sci Sports Exerc 1986;18: 107-13.         [ Links ]

8. Nakamura FY. Predições do modelo de potência crítica quanto à ocorrência da exaustão em exercício intermitente. [Dissertação]. Rio Claro: Universidade Estadual Paulista, 2001.         [ Links ]

9. Bar-Or O. The Wingate anaerobic test. An update on methodology, reliability and validity. Sports Med 1987;4:381-4.         [ Links ]

10. Withers RT, Sherman WM, Clark DG, Esselbach PC, Nolan SR, Mackay MH, et al. Muscle metabolism during 30, 60 and 90s of maximal cycling on an air-braked ergometer. Eur Appl Physiol 1991;63:354-62.         [ Links ]

11. Yamamoto M, Kanehisa H. Dynamics of anaerobic and aerobic energy supplies during sustained high intensity exercise on cycle ergometer. Eur J Appl Physiol 1995;71:320-5.         [ Links ]

12. Dudek IM, Antonelli JM, Colodel HA, Esther EF, Trindade-Junior Z, Zontta C, et al. Nível de acidose sanguínea no teste de Wingate e em exercícios supramáximos de 5 e 45 segundos. Anais do IV Congresso Sul-Brasileiro de Medicina do Esporte. Blumenau: RBME, 2002;p.13.         [ Links ]

13. Skinner JS, Morgan DW. Aspects of anaerobic performance. In: American Academy of Physical Education. Limits of human performance. Champaign: Human Kinetics, 1985;31-44.         [ Links ]

14. De-Oliveira FR, Gagliardi JFL, Kiss MAPDM. Proposta de referências para a prescrição de treinamento aeróbio e anaeróbio para corredores de média e longa duração. Rev Paul Ed Fís 1994;8:68-76.         [ Links ]

15. Simões HG. Comparação entre protocolos de determinação do limiar anaeróbio em testes de pista para corredores [Dissertação]. São Carlos: Universidade Federal de São Carlos, 1997.         [ Links ]

16. Mader A, Liesen H, Heck H, Philippi A, Rost R, Schürch P, et al. Zur beurteilung der sportartspezifischen ausdauerleistungsfähigkeit im labor. Sportarzt Sportmed 1976; 27:80-8, 109-12.         [ Links ]

17. Thomas JR, Nelson JK. Métodos de pesquisa em atividade física. 3ª ed. Porto Alegre: Artmed, 2002.         [ Links ]

18. Berg A, Keul J. Comparative performance diagnostics of anaerobic exertion in laboratory and field exercise of decathletes. Int J Sports Exerc 1985;6:244-53.         [ Links ]

19. Fujitsuka NT, Yamamoto T, Ohkuwa T, Saito M, Miyamura M. Peak blood lactate after short periods of treadmill running. Eur J Appl Physiol 1982;48:289-96.         [ Links ]

20. Lacour JR, Bouvat E, Barthelemy JC. Post-competition blood lactate concentrations as indicators of anaerobic energy expenditure during 400m and 800m races. Eur J Appl Physiol 1990;61:172-6.         [ Links ]

21. Ohkwa T, Kats Y, Katsumata K, Nakao T, Miyamura M. Blood lactate after 400m and 3000m runs in sprint and long distance runners. Eur J Appl Physiol 1984; 53:213-8.         [ Links ]

22. Cheetam ME, Boobis H, Brooks S, Williams C. Human muscle metabolism during sprinting. J Appl Physiol 1986;61:54-60.         [ Links ]

23. Hermansen L, Vaage O. Lactate disappearance and glycogen synthesis in human muscle after maximal exercise. Am J Physiol 1977;33:E422-9.         [ Links ]

24. Freund H, Zouloumian P, Oyono-Enguelle S, Lampert E. Lactate kinetics after exercise in man. Med Sport Sci 1984;17:9-24.         [ Links ]

25. Hirvonen J, Nummella A, Rusko H, Rehunen S, Härkönen M. Fatigue and changes of ATP, creatine phosphate and lactate during the 400 m sprint. Can J Sport Sci 1992;17:141-4.         [ Links ]

26. Rieu M, Duvallet A, Scharapan L, Thieulart L, Ferry A. Blood lactate accumulation in intermittent supramaximal exercise. Eur J Appl Physiol 1988;57:235-45.         [ Links ]

27. Green HJ, Hughson RL, Thomson JA, Sharratt MT. Supramaximal exercise after training induced hypervolemia I. Gas exchange and acid-base balance. J Appl Physiol 1987;62:1944-61.         [ Links ]

28. Harrison MH. Heat and exercise: effects on blood volume. Sports Med 1986;3: 214-23.         [ Links ]

29. Medbo JI, Burges S. Effect of training on the anaerobic capacity. Med Sci Sports Exerc 1990;22:501-7.         [ Links ]

30. Kindermann W, Keul J. Lactate acidosis with different forms of sports activities. Can J Appl Sports Sci 1977;2:177-82.         [ Links ]

31. Osnes JB, Hermansen L. Acid-basic balance after maximal exercise of short duration. J. Appl Physiol 1972;32:59-63.         [ Links ]

32. Hill DW, Smith JC, Croos GH, Smith AC. Energy sources for wingate anaerobic test [abstract]. Med Sci Sport Exerc 1990;22(Suppl 2):S116.         [ Links ]

33. Gollnick P, Hermansen L. Biochemical adaptations to exercise: anaerobic metabolism. In: Wilmore J, editors. Exerc Sport Sci Rev. New York: Academic Press, 1973;1:1-43.         [ Links ]

34. Telford RD, Hooper LA. Specificity of aerobic and anaerobic performance. Med Sci Sports Exerc 1979;11:94.         [ Links ]

35. Weyand PG, Cureton KJ, Conley DS, Higbie EJ. Peak oxygen deficit during one- and two-legged cycling in men and women. Med Sci Sports Exerc 1993;25:584-91.         [ Links ]



Correspondence to:
Fernando Roberto de Oliveira
Laboratory of Morpho-Functional Researches Center of Physical Education and Sports Santa Catarina State University
Rua Pascoal Simone, 358
88080-350 Florianópolis, SC, Brazil
Phone: (48) 244-2324 (branch 241)
E-mail: deoliveirafr

Received in 8/4/05.
Final version received in 8/8/05.
Approved in 14/11/05.

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License