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Print version ISSN 1517-8692
Rev Bras Med Esporte vol.18 no.1 São Paulo Jan./Feb. 2012
EXERCISE AND SPORTS SCIENCES
Filippe Falcão-TebasI; Amanda Thereza TobiasI; Adriano Bento-SantosII; José Antônio dos SantosII; Diogo Antônio Alves de VasconcelosI; Marco Antônio FidalgoIII; Raul Manhães-de-CastroI; Carol Góis LeandroIII
INutrition Department, Federal University of Pernambuco Recife, Pernambuco
IINeuropsychiatry and Behavior Sciences Department, Federal University of Pernambuco Recife, Pernambuco
IIIPhysical Education and Sports Sciences Group CAV Federal University of Pernambuco Vitória de Santo Antão, Pernambuco
The incompatibility of perinatal undernutrition and adequate nutrition during development increases the risk of early onset of non-communicable diseases in adulthood. However, it has been considered that maternal physical activity may attenuate these effects. This study aimed to evaluate the effects of physical training during pregnancy on body weight gain, waist circumference, glycaemia and cholesterolemia in adult offspring submitted to perinatal undernutrition. Female Wistar rats (n = 12) were divided into four groups: control (C, n = 3), trained (T, n = 3), undernourished (U, n = 3) undernourished and trained (T+U, n = 3). During gestation and lactation, U and T+U groups were fed a low protein diet (8% casein) and C and T groups fed a normal protein diet (17% casein). The protocol of moderate physical training was performed on a treadmill (5 days/week, 60 min/day, at 65% of VO2max) and began 4 weeks before pregnancy. At pregnancy, the duration and intensity of training were reduced (5 days/week, 20 min/day, at 30% VO2max) until the 19th prenatal day. At weaning, male pups (CP = 9, TP = 9, UP = 7, T+UP = 9) received standard diet and evaluations took place at 270 days old. Abdominal circumference (AC) was evaluated in relation to body weight. Enzymatic colorimetric method glucose-oxidase/peroxidase and cholesterol-oxidase was used to evaluate fasting glycaemia and cholesterolemia, respectively. Rats from UP group showed high body weight gain during growth, higher AC, glycaemia and cholesterolemia values when compared to CP. Concerning the T+UP group, body weight gain was attenuated, and the AC, glycaemia and cholesterolemia were normalized (p<0.05). These results demonstrate that physical training during pregnancy reduces the effects of perinatal undernutrition on some murinometric and biochemical indicators of adult offspring.
Keywords: phenotypic plasticity, low-protein diet, physical exercise.
Fetal growth and development depend on genetic, hormone and placental factors, the maternal milieu as well as adequate supply of oxygen and nutrients1. Particularly, inadequate supply of nutrients during fetal life has been associated with low weight at birth and deficit during growth and maturation2. Likewise, incompatibility between undernutrition during the perinatal period and nutrition throughout life may be related to the onset of metabolic diseases2. Perinatal undernutrition negatively influences the nervous system development, causes delay in reflex ontogenesis in breast feeding rats and alters the eating behavior in adulthood3,4. Moreover, there are alterations in the cardiac muscle morphology, delay in the acquisition of the normal patterns of locomotor activity and deficiency in the contractile elastic properties of the skeletal muscle of adult rats3-7.
The term "programming", or "phenotypic plasticity", has been used to explain that during ontogenesis, the development of each organ or system goes through a critical window of sensitivity or plasticity, in which the environmental factors may generate adjustments in the phenotype which remain throughout life8. Maternal physical activity induces to physiological adaptations during pregnancy involving the fetal-placental growth and increase of availability of nutrients and oxygen for the fetus9,10. However, such effects on the oxygenation and fetal-placental growth are directly correlated with the physical fitness level of the mother and in which moment during pregnancy the exercise program is performed11.
According to the American College of Obstetricians and Gynecologists12, women with low-risk pregnancy may practice moderate physical exercise (up to70% of O2max) and light exercise (up to 40% of O2max) for about 30 minutes a day, all days of the week. However, it is still difficult to establish percentage of physical exertion recommendations during pregnancy since intensity, kind and duration of the physical exercise are determinant for the physiological adaptations in the mother and the reflections on the offspring13. Epidemiological studies performed in a rural community in India with pregnant women demonstrated an inverse relationship between exertion intensity and weight at kids' birth14,15. In animals, female rats trained before pregnancy (five days/week, 60 min/day, at 65% of O2max) with progressive decrease of exertion during pregnancy (five days/week, 30 min/day, at 40% of O2max) presented less remarkable decrease in oxygen consumption at rest16. This effect was determinant to attenuate the effects of perinatal protein undernutrition related to the maturation of the nervous system and somatic growth rate of the pups16,17.
The studies which associate physical training during pregnancy with the phenotypic plasticity hypothesis are still scarce. In addition to that, little is known about the long term consequences of a physical training program during pregnancy. Thus, the present study had the aim to evaluate the effects of physical training during pregnancy in the ponderal evolution, abdominal circumference, glycaemia and cholesterolemia of adult offspring submitted to perinatal undernutrition. Our hypothesis is that physical training during pregnancy attenuates the effects of the programming induced by perinatal protein undernutrition.
MATERIAL AND METHODS
This study was approved by the Ethics in Studies with Animals Committee of the Center of Biological Sciences of UFPE (protocol # 23076.049077/2010-80). Animal manipulation and care followed the guidelines by the Brazilian Committee of Animals Experimentation (COBEA).
12 Wistar albino female rats (60 days old), body weight between 180 ± 11g, obtained in the Nutrition Department of the Federal University of Pernambuco were used. The rats were kept in an animal facility with standard conditions of temperature of 23ºC ± 1 and light cycle from 18:00 to 6:00h, with free access to water and standard food from the animal facility (52% carbohydrates, 21% proteins, 4% lipids Nuvilab CR1-Nuvital®, Curitiba, Paraná, Brazil). The rats were divided in two experimental groups: control (C, n = 6) and trained (T, n = 6). Group T performed a moderate physical training programon treadmill (EP-131®, Insight Equipments, SP, Brazil)16 (table 1). After the four-week physical training, the rats fromthe two groups were placed to mate (two females for one male). The pregnancy diagnosis was performed through vaginal smear assay for sperm presence18. Once pregnancy is detected, half of the rats of each group (C and T) was submitted to hypoprotein diet (8% casein), while the other rats received normoprotein diet (17% casein). The diets designed were isocaloric, with alteration only in the protein content19, consisting of the following groups: control (C, n = 3, 17 % casein); trained (T, n = 3, 17% casein); undernourished (U, n = 3, 8% casein) and trained undernutured (T+U, n = 3, 8% casein). The physical training program was kept during pregnancy until the 19th day, with progressive intensity and duration of the sessions decrease (table 2). During the entire experiment the maternal body weight was weekly followed. The mothers kept receiving the casein-based experimental dietand the litters were adjusted to six pups per mother. After weaning (at 22 days of life of the pups), three male pups of each litter were randomly used. The pups were fed with standard diet of the animal facility and divided in four groups according to the manipulation of their respective mothers: pups from control mothers (CP, n = 9), pups from trained mothers (TP, n = 9), pups from undernourished mothers (UP, n = 7) and pups from trained and undernourished mothers (T+UP, n = 9).
Physical training protocol
The animals were previously adapted to the animal facility inverted cycle (during 15 days) and to the treadmill for three days (10 min/day, with velocity of 0.3km.h-1). The moderate physical training used consisted of four training weeks, five times per week, 60 minutes perday at 65% of O2max16. Adaptation to physical training took place on the first week, which consisted of 20-minute sessions, divided in four stages of five minutes, during five days. After the adaptation, the protocol was divided in progressive stages in each session: 1) warm-up (five minutes); 2) intermediate zone (10 minutes); 3) training zone (30 minutes); and 4) final period (five minutes) (table 1).
In the gestation period, progressive decrease in training intensity and duration has occurred. Training consisted of three weeks of training, five days per week, being reduced to 20 minutes per day at 30% of O2max (table 2).
Body weight and weight gain evaluation
Body weight expressed in grams was weekly evaluated in the mothers and monthly in the pups on a digital electronic scale Marte, model S-1000, with maximum capacity of 1,000g and sensitivity of 0.01g. The weight gain percentage was calculated using the weaning weight as reference according to the formula: % weight gain = [weight of the day (g) x 100/weaning weight (g)] 10020. Abdominal circumference was determined by the highest circumference between the upper iliac crest and the last rib and expressed in centimeters21.
Glycaemia and cholesterolemia evaluation of the pups
After six-hour fast, the animals had a cut in the tail tip for blood collection. The serum glucose and cholesterol (mg.dL-1) of the pups at 270 days of age were assessed. The blood glucose concentrations were identified by glucose-oxidase/peroxidase colorimetric enzymatic analysis and the reading by a glucometer (AccuChek Performa®, Roche Diagnostics). The cholesterolemia amount was quantified by cholesterol-oxidase method and analyzed by photometry with the values being calculated in the monitor (Accutrend Cholesterol®, Roche Diagnostics).
Initially, all variables were submitted to the normality test (Kolmogorov-Smirnov).When variances normality and homogeneity were revealed, Student's ttest was used for comparison of parameters of the female rats during the pre-gestation period.Inter-group analysis during pregnancy, weight of the pups at birth and during their growth and evaluation of the biochemical parameters of the animals at adulthood was performed with two-way ANOVA followed by Bonferronipost hoctest. The values are expressed in mean and standard error of the mean (SEM). Significance level was set at 5% (p < 0.05) in all cases. The entire statistical analysis was performed using the GraphPad Prism®program (GraphPad Software Inc., La Jolla, CA, USA; version 5.0 for Windows).
The rats submitted to the moderate physical training protocol presented lower body weight gain values in the pre-gestation period from the third week (table 3).
During pregnancy, the groups were subdivided according to the diet offered to the mothers. The mothers that received hypocaloric diet presented lower body weight gain from the second week of pregnancy. The trained mothers that were undernourished presented lower weight gain values in the last week of pregnancy (table 4).
The mean weight value at birth of the pups from undernourished mothers was lower when compared to the control (CP = 6.1 ± 0.3; UP = 4.9 ± 0.3; TP = 7.1 ± 0.4; T + UP= 5.9 ± 0.2).The pups' growth was followed from weaning until 270 days of life. The animals from undernourished and trained undernourished mothers presented lower values of body weight during growth (figure 1A). However, weight gain concerning weaning of the undernourished animals was higher from the 30th day of life, when compared to the control (figure 1B). Although the animals from the trained undernourished mothers had presented higher body weight gain during growth, these values were lower than the undernourished group (figure 1B).
The mean values of the abdominal circumference concerning body weight of the pups from undernourished mothers were higher compared to the control. In the group of animals from trained undernourished mothers there was not difference compared to the control and it was lower compared to the undernourished group (figure 2).
Glycaemia and cholesterolemia of the pups in adulthood which were submitted to perinatal undernutrition were higher compared to the control group (figure 3). The animals from trained undernourished mothers did not present difference compared to the control and presented mean values lower than the undernourished group (figure 3).
The aim of the present study was to evaluate the moderate physical training effects in the body weight gain of the mothers submitted or not to perinatal undernutirtion, as well as the consequences in the 270 day-old pups. Corroborating our hypothesis, maternal physical training during pregnancy attenuated the deleterious effects of perinatal undernutrition in the ponderal evolution and murinometricand biochemical indicators in adult rats.
Concerning the undernourished mothers, it was observed lower body weight gain and that their pups were born with low weight, corroborating previous studies20,22,23. The pups from undernourished mothers remained with lower growth trajectory until adulthood even when the balanced diet was offered. It is interesting to observe that body weight gain (expressed in percentage from weaning) was higher in the pups from undernourished mothers. This phenomenon is named catch up growth, in which the body compensates in the long term for a slow growth rate by protein deficit in diet during the initial periods of life24. This post-natal rapid growth has been associated with increase of the percentage of adipose tissue, blood pressure as well as risk of developing glucose intolerance in long-term25.
On the other hand, the alterations in the catch up of the undernourished animals were attenuated in the pups from trained undernourished mothers, demonstrating hence that physical training may attenuate the effects of hypoprotein undernurtrition. In the present study, the rats initiated the physical training protocol four weeks before pregnancy, in order to normalize the physiological parameters in response to the acute stress of an exercise session. Moreover, physical exercises of light intensity during pregnancy, similar to the ones used in our investigation, have been recommended, since, besides contributing to the maintenance of weight gain, they increased the fetal-placental growth rate and weight at birth11. The subjacent mechanisms seem to include: a) increase of uterine blood flow; b) redistribution of blood flow; c) alterations in the fetal and placental production of hormones which control growth; and d) lower decrease of oxygen consumption at rest in response to physical training9,16,26. These physiological parameters may be contributing to a better predictive adaptation response (a theoretical model which supports the hypothesis of the phenotypic plasticity) generated by the early stimulus8. This response has been associated with epigenetic mechanisms, in which an external factor may modulate the DNA structure with no change in its sequence2,8. In rats exposed to intrauterine undernutrition, higher leptin, insulin and peptide-C concentrations, indicators of the metabolic syndrome in adulthood, due to alterations in the genotype of animals have been observed27. Thus, it has been reported that early environmental stimuli may modulate the phenotype throughout life.
The abdominal circumference has been used as an indicator of obesity in rats21. In population studies, the abdominal circumference has been used as an indicator of risk of cardiovascular diseases and obesity onset in adults. According to the phenotypic plasticity hypothesis, the animals submitted to perinatal undernutrition presented higher values of abdominal circumference and our results re similar to the ones in previous studies28,29. In rats, it has been demonstrated that newborn pups from undernourished mothers (50% of restriction of the diet received by the control ad libitum) presented at nine months of age increase in the expression of lipogenic and adipogenic transcription factors, leading to adipocyte hypertrophy30. Conversely, in the animals from trained undernourished mothers, these results were attenuated. It is probable that the lower catch up growth observed in these animals may justify lower abdominal circumference.
Increase in abdominal circumference may also indicate higher plasma concentration of triglycerides, fatty acids and cholesterol, which are indicators of atherogenic risk31. Our results demonstrate that offspring of undernourished mothers presented increase in glucose and cholesterol serum concentrations when adults. These effects have been verified in previous studies and are classic indicators of the beginning of peripheral resistance to insulin and dyslypidemias associated with the developmental origin of the metabolic syndrome32. However, the animals from trained undernourished mothers did not present this hyperglyceamia and hypercholesterolemia scenario.
Therefore, some mechanisms which can theoretically support the physical training attenuation activity can be suggested: increase in placental volume, increase in the blood flow to the placenta after the exercise, increase in the passage of nutrients and oxygen to the fetus, increase in the maternal body lean mass33and increase in the oxygen consumption at rest16. It is worth mentioning that all these effects are directly related with the effort magnitude. In the present study, a standard training protocol from the direct measurements of oxygen consumption was used. The intensity and duration were controlled so that the training sessions could be of moderate and light intensity16.
In conclusion, moderate physical training before and during pregnancy attenuated the perinatal undernutrition effects on the somatic growth and glucose and cholesterol serum levels in the adult pups. Our data corroborate the studies which test the phenotypic plasticity hypothesis and open a scenario for the effects of a positive environmental stimulus, being able to attenuate the undernutrition effects.
To The National Council for Scientific and Technological Development (CNPq) and Foundation for the Support of Science and Technology of the State of Pernambuco (FACEPE) for making this study possible.
1. Harding JE. The nutritional basis of the fetal origins of adult disease. Int J Epidemiol 2001;30:15-23. [ Links ]
2. Bateson P, Barker D, Clutton-Brock T, Deb D, D'Udine B, Foley RA, et al. Developmental plasticity and human health. Nature 2004;430(6998):419-21. [ Links ]
3. Barros KM, Manhaes-De-Castro R, Lopes-De-Souza S, Matos RJ, Deiro TC, Cabral-Filho JE, et al. A regional model (Northeastern Brazil) of induced mal-nutrition delays ontogeny of reflexes and locomotor activity in rats. Nutr Neurosci 2006;9:99-104. [ Links ]
4. Lopes de Souza S, Orozco-Solis R, Grit I, Manhaes de Castro R, Bolanos-Jimenez F. Perinatal protein restriction reduces the inhibitory action of serotonin on food intake. Eur J Neurosci 2008;27:1400-8. [ Links ]
5. Toscano AE, Amorim MA, de Carvalho Filho EV, Aragao Rda S, Cabral-Filho JE, de Moraes SR, et al. Do malnutrition and fluoxetine neonatal treatment program alterations in heart morphology? Life Sci 2008;82:1131-6. [ Links ]
6. Toscano AE, Manhaes-de-Castro R, Canon F. Effect of a low-protein diet during pregnancy on skeletal muscle mechanical properties of offspring rats. Nutrition 2008;24:270-8. [ Links ]
7. Orozco-Solis R, Lopes de Souza S, Barbosa Matos RJ, Grit I, Le Bloch J, Nguyen P, et al. Perinatal undernutrition-induced obesity is independent of the developmental programming of feeding. Physiol Behav 2009;96:481-92. [ Links ]
8. Gluckman PD, Hanson MA. Developmental plasticity and human disease: research directions. J Intern Med 2007;261:461-71. [ Links ]
9. Clapp JF 3rd, Schmidt S, Paranjape A, Lopez B. Maternal insulin-like growth factor-I levels (IGF-I) reflect placental mass and neonatal fat mass. Am J Obstet Gynecol 2004;190:730-6. [ Links ]
10. Haakstad LA, Voldner N, Henriksen T, Bo K. Physical activity level and weight gain in a cohort of pregnant Norwegian women. Acta Obstet Gynecol Scand 2007;86:559-64. [ Links ]
11. Clapp JF, 3rd. Long-term outcome after exercising throughout pregnancy: fitness and cardiovascular risk. Am J Obstet Gynecol 2008;199:489.e1-6. [ Links ]
12. Leandro CG, Amorim MF, Hirabara SM, Curi R, Castro RMd. Can maternal physical activity modulate the nutrition-induced fetal programming? Revista de Nutrição 2009;22:559-69. [ Links ]
13. ACOG Committee opinion. Number 267, January 2002: exercise during pregnancy and the postpartum period. Obstet Gynecol 2002;99:171-3. [ Links ]
14. Dwarkanath P, Muthayya S, Vaz M, Thomas T, Mhaskar A, Mhaskar R, et al. The relationship between maternal physical activity during pregnancy and birth weight. Asia Pac J Clin Nutr 2007;16:704-10. [ Links ]
15. Rao S, Kanade A, Margetts BM, Yajnik CS, Lubree H, Rege S, et al. Maternal activity in relation to birth size in rural India. The Pune Maternal Nutrition Study. Eur J Clin Nutr 2003;57:531-42. [ Links ]
16. Amorim MF, dos Santos JA, Hirabara SM, Nascimento E, de Souza SL, de Castro RM, et al. Can physical exercise during gestation attenuate the effects of a maternal perinatal low-protein diet on oxygen consumption in rats? Exp Physiol 2009;94:906-13. [ Links ]
17. Falcão-Tebas F, Bento-Santos A, Fidalgo MA, Almeida MB, Santos JA, Lopes de Souza S, et al. Maternal low protein diet-induced delayed reflex ontogeny is attenuated by moderate physical training during gestation in rats. Br J Nutr. 2011; in press. [ Links ]
18. Marcondes FK, Bianchi FJ, Tanno AP. Determination of the estrous cycle phases of rats: some helpful considerations. Braz J Biol 2002;62:609-14. [ Links ]
19. Reeves PG, Nielsen FH, Fahey GC Jr. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 1993;123:1939-51. [ Links ]
20. Bayol S, Jones D, Goldspink G, Stickland NC. The influence of undernutrition during gestation on skeletal muscle cellularity and on the expression of genes that control muscle growth. Br J Nutr 2004;91:331-9. [ Links ]
21. Novelli EL, Diniz YS, Galhardi CM, Ebaid GM, Rodrigues HG, Mani F, et al. Anthropometrical parameters and markers of obesity in rats. Lab Anim 2007;41:111-9. [ Links ]
22. Lucas A, Baker BA, Desai M, Hales CN. Nutrition in pregnant or lactating rats programs lipid metabolism in the offspring. Br J Nutr 1996;76:605-12. [ Links ]
23. Ozanne SE, Hales CN. The long-term consequences of intra-uterine protein malnutrition for glucose metabolism. Proc Nutr Soc 1999;58:615-9. [ Links ]
24. Barker DJ, Eriksson JG, Forsen T, Osmond C. Fetal origins of adult disease: strength of effects and biological basis. Int J Epidemiol 2002;31:1235-9. [ Links ]
25. Hales CN, Ozanne SE. The dangerous road of catch-up growth. J Physiol 2003;547(Pt 1):5-10. [ Links ]
26. Clapp JF, 3rd, Kim H, Burciu B, Schmidt S, Petry K, Lopez B. Continuing regular exercise during pregnancy: effect of exercise volume on fetoplacental growth. Am J Obstet Gynecol 2002;186:142-7. [ Links ]
27. Morris TJ, Vickers M, Gluckman P, Gilmour S, Affara N. Transcriptional profiling of rats subjected to gestational undernourishment: implications for the developmental variations in metabolic traits. PLoS One 2009;4:e7271. [ Links ]
28. Ozanne SE, Hales CN. Lifespan: catch-up growth and obesity in male mice. Nature 2004;427(6973):411-2. [ Links ]
29. Vuguin PM. Animal models for small for gestational age and fetal programming of adult disease. Horm Res 2007;68:113-23. [ Links ]
30. Ozanne SE, Hales CN. Early programming of glucose-insulin metabolism. Trends Endocrinol Metab 2002;13:368-73. [ Links ]
31. Taylor PD, Poston L. Developmental programming of obesity in mammals. Exp Physiol 2007;92:287-98. [ Links ]
32. Ozanne SE. Metabolic programming in animals. Br Med Bull 2001;60:143-52. [ Links ]
33. Thomas DM, Clapp JF, Shernce S. A foetal energy balance equation based on maternal exercise and diet. J R Soc Interface 2008;5:449-55. [ Links ]
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Rua Prof. Moraes Rego, 1.235 Departamento de Nutrição Cidade Universitária 50670-901 Recife, PE, Brasil.
All authors have declared there is not any potential conflict of interests concerning this article.