SciELO - Scientific Electronic Library Online

 
vol.18 número2Cardiovascular behavior after resistance exercise performed in different work ways and volumeUse of resistance exercise as a factor antagonized by naloxone of analgesia in acute knee synovitis in Wistar rats índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Articulo

Indicadores

Links relacionados

  • No hay articulos similaresSimilares en SciELO

Compartir


Revista Brasileira de Medicina do Esporte

versión impresa ISSN 1517-8692

Rev Bras Med Esporte vol.18 no.2 São Paulo marzo/abr. 2012

http://dx.doi.org/10.1590/S1517-86922012000200012 

ORIGINAL ARTICLE
EXERCISE AND SPORTS SCIENCES

 

Blood lactate responses to high-intensity intermittent training in rats

 

 

Ana Carolina Panveloski-CostaI; Marcelo PapotiII; Rafael Junges MoreiraI; Patricia Monteiro SeraphimI

IPhysiotherapy Department, Laboratory of the Research Group in Physiology, Júlio de Mesquita Filho State University of São Paulo UNESP – Presidente Prudente Campus
II
Physical Education Department,Júlio de Mesquita Filho State University of São Paulo UNESP – Presidente Prudente Campus

Mailing address

 

 


ABSTRACT

During high-intensity intermittent muscle contractions for short periods of time there is an important involvement of glycolytic metabolism and consequent increased blood lactate concentrations. This study aimed to evaluate the blood lactate responses in Wistar rats submitted to high-intensity intermittent training (jump squat) protocol during 6 weeks, 3 sessions, 12 x/session, 60s of interval between sessions. There was significant increase of blood lactate concentrations during the acute bout of high-intensity intermittent exercise (basal blood lactate vs blood lactate after last effort, P<0.001); however, after six weeks of training, there was significant reduction (49%) in blood lactate response to the exercise in comparison to the first session, P=0.0002. The high-intensity intermittent exercise performed at intervals of 60 seconds stimulated the glycolytic system; nevertheless, the training promoted reduction in blood lactate responses to high-intensity intermittent protocol, suggesting hence improvement in phosphocreatine recovery capacity and in mitochondrial biogenesis.

Keywords: resistance training, blood lactate, glycolytic system.


 

 

INTRODUCTION

In animals models, the aerobic training protocols have been frequently used on treadmill1 and swimming2. Concerning the anaerobic training protocols, many studies have used efforts with jumps performed in water3,4 and on platform through eletric stimulation5,6in different nutritional7and pathological conditions3. The training protocols with jumps are composed of 3-4 sets of 10-12 jumps with intervals of 60-90s6,7. Additionally, the intensity increase is performed with weight addition corresponding to the body weight4 or a maximum repetition8.

High-intensity physical training performed for short periods of time has significant effects on the anaerobic glycolytic system, in a way that the increase of the ATP production is followed by increase of muscular lactate production9.

The use of blood lactate concentrations has been an instrument constantly used as a marker of exercise intensity in humans9and in animal models2. In addition to that, this metabolite has been recently used to indirectly estimate the contribution of the lactic anaerobic metabolism in the energetic supply10as well as evaluation of the alactic anaerobic metabolism11.

However, opposed to the aerobic training models, which are often characterized through the lactacidemic response and tests of aerobic capacity, the availability of studies about the lactacidemic response and consequently the participation of the glycolytic system and the consequent adaptations to this condition in exercises, which predominantly use the glycolytic metabolism for energy, are limited. Thus the aim of the present study was to investigate the lactacidemic responses and the participation of the glycolytic system during acute and chronic training of Wistar rats submitted to a jump squat high-intensity intermittent training model.

 

MATERIAL AND METHODS

Animals

Ten Wistar male adult rats (120 days) were kept in the experimentation animal facility in the origin university, in collective cages, not exceeding four animals per cage, in light/dark cycle of 12/12 hours and controlled temperature of 22 ± 2ºC. The animals were fed with standard food (Supra Lab – Alisul Ind. Alimentos LTDA., São Leopoldo, RS) and received water ad libitum. The experimental procedures used in the present study were approved by the Ethics in Animal Experimentation Committee of UNESP – Presidente Prudente Campus, law suit # 15/2009.

Training program

The high-intensity intermittent training was performed following the strength model adapted by Tamaki et al. (1992). The apparatus was designed so that the animal was immobilized on a metallic platform through an adapted vest attached to the animal's thorax (Figure 1). In order to make the rat jump (complete knee and ankle flexo extension movement) lifting a load positioned on the posterior part of the vest, electric stimulation was applied using a metallic clip which involved the  animal's tail tip connected to an electro stimulator, model Dualpex 961 by Quark, calibrated by Inmetro (National Metrology Institute, Industry Normalization and Quality)6. The used parameters were: frequency 1Hz, duration of 0.3s with 2s interval between each electric stimulation, and intensity was adjusted so that the animal performed the movement, ranging from 3 to 6mA. These parameters were adopted for being bidirectional pulses of null mean, not presenting electrolytic effects, allowing long duration applications with no risk of tissue injury5.

 

 

Experimental outlining

The animals were adapted to the exercise model one week before the beginning of the high-intensity intermittent training. The adaptation consisted in the performance of three sessions with no load increase composed of one, two and three sets of 12 repetitions from the first to the third day, respectively, with 24-hour interval between each session.

Subsequently, they were submitted to six weeks of high-intensity intermittent training.On the two first weeks the training was performed with no load increase and from the third week equivalent load to 50% of body weight (BW) was added, which was weekly monitored.

the trianing protocol consisted of three sessions a week which were performed in 24 hours intervals among them. each session consisted of three sets of 12 repetitions and 60s of intervals among the sets.

Blood collection and analys is

At every 15 days (six sessions), the lactacidemic responses were monitored during andat the end of the training. The blood samples were collected immediately after the first ([La-]), second ([La-]) and third ([La-]) sets and at the third (3rd min), fifth (5th min) and seventh (7th min) minute after the last jump set, and the highest lactacidemic value obtained at the end of the stimuli was taken as lactate peak concentration ([La-]peak).

The blood samples (25µL) were collected by punction on the tale in a heparinized capillary and were immediately transferred to 1,5 mL Eppendorf tubes containing 50µL of NaF solution at 1%. The homogenized was later frozen for subsequent analysis in Yellow Springs electro enzymatic lactimeter, model 1500 Sport (Figure 2).

 

STATISTICAL ANALYSIS

Data normality was confirmed with Shapiro-Wilk test. Body weight values and lactacidemic responses during the acute physical exercise session were compared and during six weeks of high-intensity intermittent training the one-way ANOVA for repeated measures was used, followed by Tukeypost hoc test whenever necessary.  In all cases the significance level was set at P < 0.05.

 

RESULTS

Significant alterations in the BW of the animals were not observed during the training period with weight ranging in about 400.88 ± 13.93g (n = 10 animals).  During the acute session, significant increase of 29%, 86% and 140% in [La-]1st, [La-]2ndand [La-]3rd, respectively, concerning rest was observed. Moreover, the [La-]peak was significantly higher than the values observed in rest and [La-]1st, [La-]2nd and [La-]3rd (Figure 3). However, significant differences were not observed between the lactacidemic values observed on the third, fifth and seventh minute.  Mean significant reduction of 32% in [La-]1st,  46% in [La-]2nd  and  48% [La-]3rd   in  T2, T3 and T4 was observed when compared with T1 (P = 0.0002).  The [La-]1st  in T3 and T4 was 24% and 25%, respectively, lower concerning [La-]1st in  T2 (P <0.05). Additionally, the [La-]2nd in T4 was significantly lower (25%) compared with T3 (P <0.05). Nevertheless, the [La-]3rdpresented significant difference in T2, T3 and T4 only when compared with T1 (figure 4).

 

 

 

 

The [La-]peak obtained in T2, T3 and T4 presented reduction of approximately 35%, 39% and 49%, respectively, compared with the one obtained in T1 (P = 0.0002). Moreover, the [La-]peak in T4 presented 22% of reduction compared withT2 (P = 0.004) (Figure 5).

 

 

DISCUSSION

Physical activity practice represents an important instrument for reversal of metabolic alterations of many diseases such as obesity, type 2 diabetes mellitus and cardiorespiratory diseases12.

The blood lactate concentrations let us evaluate the predominance of the participation of the aerobic or anaerobic system in response to an acute9 or chronic physical exercise13. Therefore, they have been used for prediction of the physical exercise intensity with the goal to optimize performance and/or minimize the pathological metabolic alterations as wellas to evaluate the metabolic responses to training14.

During the high-intensity exercise initial phases, the ATP is degraded through the ATPase myosin enzyme, while the phosphocreatine is degraded by the creatine kinase enzyme for the ATP resynthesis. In order to perform intense exercise longer than 12-15 seconds and shorter than three minutes of duration, the body mainly depends on the anaerobic metabolism for energy production14. When a high-intensity physical training is performed in short time periods, the increase of ATP production is followed by increase of the muscular lactate production9.

In the present study, during the high-intensity intermittent training session, it can be observed that the lactacidemic response presented progressive increase during the time, with peak lactate concentrations at the seventh recovery minute (Figure 3). These observations demonstrate that the time interval adopted between each set (60 seconds) was not sufficient to cause complete replacement of the phosphor creatine (PCr) supplies, so the glycolytic system effectively participated in the ATP production during the high-intensity intermittent training session.

Increase in the blood lactate concentration was also observed by Gorostiaga et al.15 associated with the lower PCr contribution after 10 repetition maximum (RM) of knee extension (leg press) when compared with a 5RM set. Still in this study, the exercise protocol produced fatigue and alteration in the muscular PCr content, lactate and glycolytic intermediaries in higher levels15.

The higher values of blood lactate concentrations [La-]1st, 2nd [La-]2ndª and 3rd[La-]3rd and [La-]peak were observed on the first day of collection; other words, in the first training session (Figure 4). It is known that the adrenaline and noradrenaline plasma concentrations increase during physical exercise16and strong correlation between the catecholamine and lactate plasma concentrations has been reported17.

It is probable that in the first training session of the present study, although the animals have been previously adapted to the experimental model, higher activation of the β-adrenergic system has occurred, which may have influenced on the higher lactacidemic response in this training session when compared with the subsequent sessions (Figure 4).

Physical training may result in alterations in the ATP and PCr levels stored in the muscle. Strength training promotes increase of approximately 20% of ATP and PCr18.Larsen et al.19 verified that he constant PCr recovery after performance of maximal isometric voluntary contraction of the tibialis anterior and vastus lateralis muscles with reduction of 50% of the PCr basal values, in active individuals is higher than in sedentary individuals. Likewise, Yoshida20 observed higher recovery velocity of PCr (63%) in the biceps femoris muscle of young runners when compared with sedentary young subjects. This difference magnitude in the constant recovery of PCr was similar to the one found by Larsen et al.19.

Expressive decrease in the lactate peak concentration was observed on the last collection day of blood lactate when compared with the second day. This reduction of peak lactate concentration after six weeks of high-intensity intermittent training suggests improvement of the PCr resynthesis system; that is, increase of the muscular PCr supplies.

The PCr resynthesis rate is directly proportional to the oxidative phosphorylation rate and therefore, it reflects contributions both of glycolytic and oxidative metabolism21,22. In agreement with this statement, Mogensen et al.23 reported mitochondrial respiration rates and citrate syntase activity 41% higher in the vastus lateralis muscle of trained individuals when compared with untrained ones.

Forbes et al.22also used the intervalled maximal exertions protocol and demonstrated improvement in the PCr resynthesis constant in the quadriceps of adults after two weeks of training.

Although improvement in oxidative capacity as a result of aerobic training is a consensus in the exercise physiology field, recent studies have suggested that exercises performed at critical intensities induce alterations in the oxidative capacity24. In a study carried out in young individuals, six weeks of intervalled maximal efforts induced improvement in markers of oxidative capacity in the vastuslateralis muscle, similarly to in an aerobic training25.

According to our results, it can be concluded that the reduction of the lactacidemic values in response to this training model was promoted by increase, especially of the PCr supplies (Figure 5). However, considering that high-intensity efforts performed in a short period of time stimulate the mitochondrial biogenesis by increase in PGC1-α expression (peroxisome proliferator-activated receptor-gamma coactivator 1 alpha), a transcriptional factor involved in the gene regulation of the cellular energetic metabolism, as proposed by Gibala24, the low lactacidemic response in this model may also be a result from the improvement in the oxidative capacity.

Thus, this experimental model let us propose that high-intensity intermittent physical training may be used to promote adaptations in metabolism with simultaneous improvement of use of energetic substrate due to improvement in mitochondrial biogenesis.

This study let us conclude that in the experimental model of high-intensity intermittent physical exercise there is significant participation of the glycolytic system. However, if chronically performed, high-intensity intermittent training promotes alterations in the energetic metabolism which implies in reduction of the participation of this system in the ATP production.

 

ACKNOWLEDGEMENTS

We acknowledge Professor Luiz Carlos Vanderlei Marques and Professor José Carlos Silva Camargo Filho for the availability in the use of the animal facility in the FCT-UNESP; FAPESP 2004/10130-0; and FUNDUNESP 795/2010.

 

REFERENCES

1. Yaspelkis BB, Kvasha IA, Figueroa TY. High-fat feeding increases insulin receptor and IRS-1 coimmunoprecipitation with SOCS3, IKKalpha/beta phosphorylation and decreases PI-3 kinase activity in muscle. Am J Physiol Regul Integr Comp Physiol 2009;296:1709-15.         [ Links ]

2. Araujo GG, Papoti M, Gobatto-Manchado FB, Mello MAR, Gobatto CA. Padronização de um protocolo experimental de treinamento periodizado em natação utilizando ratos Wistar. Rev Bras Med Esporte 2010;19:51-6.         [ Links ]

3. Lima C, Alves LE, Iagher F, Machado AF, Bonatto SJ, Kuczera D, et al. Anaerobic exercise reduces tumor growth, cancer cachexia and increases macrophage and lymphocyte response in Walker 256 tumor-bearing rats. Eur J Appl Physiol 2008;104:957-64.         [ Links ]

4. Souza CF, Machado AF, Bonatto SJR, Grando FCC, Pessini C, Alves LE, et al. Neutrophil response of anaerobic jump trained diabetic rats. Eur J Appl Physiol 2008;104:1079-86.         [ Links ]

5. Tamaki T, Uchiyama S, Nakano S. A weight-lifting exercise model for inducing hypertrophy in the hindlimb muscles of rats. Med Sci Sports Exer 1992;24:881-6.         [ Links ]

6. Baraúna VG, Junior MLB, Costa Rosa, LFBP, Casarini DE, Krieger JE, Oliveira EM. Cardiovascular adaptations in rats submitted to a resistance-training model. Clin Exp Pharmacol Physiol 2005;32:249-54.         [ Links ]

7. Tonon CR, Mello MAR, Dias TF, Anaruma CA. Teor Protéico da Dieta e Crescimento Muscular em Ratos Submetidos ao Treinamento Anaeróbio. Motriz 2001;7:69-74.         [ Links ]

8. Faria TO, Targueta GP, Angeli JK, Almeida EAS, Stefanon I, Vassallo DV et al. Acute resistance exercise reduces blood pressure and vascular reactivity, and increases endothelium-dependent relaxation in spontaneously hypertensive rats. Eur J Appl Physiol 2010;110:359-66.         [ Links ]

9. Laursen PB, Rhodes EC, Langill RH, McKenzie DC, Taunton JE. Relationship of exercise test variables to cycling performance in an Ironman triathlon. Eur J Appl Physiol 2002;87:433-40.         [ Links ]

10. Bertuzzi RCM, Silva AEL, Pires FO, Kiss MAPD. Visual determination of the fast component of excessive oxygen uptake after exercise. Rev Bras Med Esporte 2010;16:139-43.         [ Links ]

11. Silva ARS, Santiago V, Papoti M, Gobatto CA. Psychological, biochemical and physiological responses of Brazilian soccer players during a training program. Science & Sports 2008;23:66-72.         [ Links ]

12. Handschin C, Spiegelman BM. The role of exercise and PGC-1 in inflammation and chronic disease. Nature 2008;454:463-9.         [ Links ]

13. Wells GD, Selvadurai H, Tein I. Bioenergetic provision of energy for muscular activity. Paediatr Respir Rev 2009;10:83-90.         [ Links ]

14. Da Costa Santos VB, Ruiz RJ, Vettorato ED, Nakamura FY, Juliani LC, Polito MD, et al. Effects of chronic caffeine intake and low-intensity exercise on skeletal muscle of Wistar rats. Exp Physiol 2011;96:1228-38.         [ Links ]

15. Gorostiaga EM, Navarro-Amézqueta I, Cusso R, Hellsten Y, Calbet JAL, Guerrero M, et al. Anaerobic Energy Expenditure and Mechanical Efficiency during Exhaustive Leg Press Exercise. PLoS One 2010;19:1-11.         [ Links ]

16. Fattor JA, Miller BF, Jacobs KA, Brooks GA. Catecholamine response is attenuated during moderate-intensity exercise in response to the "lactate clamp". Am J Physiol Endocrinol Metab 2005;288:143-7.         [ Links ]

17. Krzemiński K, Kruk B, Nazar K, Ziemba AW, Cybulski G, Niewiadomski W. Cardiovascular, metabolic and plasma catecholamine responses to passive and active exercises. J Physiol Pharmacol 2000;51:267-78.         [ Links ]

18. MacDougall JD, Ward GR, Sale DG, Sutton JR. Biochemical adaptation of human skeletal muscle to heavy resistance training and immobilization. J Appl Physiol 1977;43:700-3.         [ Links ]

19. Larsen RG, Callahan DM, Foulis SA, Kent-Braun JA. In vivo oxidative capacity varies with muscle and training status in young adults. J Appl Physiol 2009;107:873-9.         [ Links ]

20. Yoshida T. The rate of phosphocreatine hydrolysis and resynthesis in exercising muscle in humans using 31P-MRS. J Physiol Anthropol Appl Human Sci 2002;21:247-55.         [ Links ]

21. Paganini AT, Foley JM, Meyer RA. Linear dependence of muscle phosphocreatine kinetics on oxidative capacity. Am J Physiol 1997;272:501-10.         [ Links ]

22. Forbes SC, Paganini AT, Slade JM, Towse TF, Meyer RA. Phosphocreatine recovery kinetics following low- and high-intensity exercise in human triceps surae and rat posterior hindlimb muscles. Am J Physiol Regul Integr Comp Physiol 2009;296:161-70.         [ Links ]

23. Mogensen M, Bagger M, Pedersen PK, Fernstrom M, Sahlin K. Cycling efficiency in humans is related to low UCP3 content and to type I fibres but not to mitochondrial efficiency. J Physiol 2006;571:669-81.         [ Links ]

24. Gibala M. Molecular responses to high-intensity interval exercise. Appl Physiol Nutr Metab 2009;34:428-32.         [ Links ]

25. Burgomaster KA, Howarth KR, Phillips SM, Rakobowchuk M, MacDonald MJ, McGee SL, Gibala MJ. Similar metabolic adaptations during exercise after low volume sprint interval and traditional endurance training in humans. J Physiol 2008;586:151-60.         [ Links ]

 

 

Mailing address:
Rua Roberto Simonsen, 305 – Centro Educacional

19060-900 – Presidente Prudente, SP
E-mail: patricia@fct.unesp.br

All authors have declared there is not any potential conflict of interests concerning this article.