Histology and histochemistry of the accessory gland of the female reproductive tract of Rhodnius neglectus Lent, 1954 (Hemiptera: Reduviidae)

Histologia e histoquímica das glândulas acessórias do sistema reprodutor feminino de Rhodnius neglectus Lent, 1954 (Hemiptera: Reduviidae)

Abstract

Rhodnius neglectus is a wild triatomine, vector of the protozoan Trypanosoma cruzi, which causes Chagas’ disease, and feeds on the blood of small mammals, being essential for its growth and reproduction. Accessory glands of the female reproductive tract are important in insect reproduction, but their anatomy and histology in R. neglectus are poorly studied. The aim of this work was to describe the histology and histochemistry of the accessory gland of the female reproductive tract of R. neglectus. The reproductive tract of five females of R. neglectus was dissected and the accessory glands transferred to Zamboni's fixative solution, dehydrated in a crescent series of ethanol, embedded in historesin, sectioned at 2 µm thick, stained with toluidine blue for histological analysis or mercury bromophenol blue for detection of total proteins. The accessory gland R. neglectus is tubular, without branches, opening in the dorsal region of the vagina and differing along its length in proximal and distal regions. In the proximal region, the gland is lined by the cuticle with a layer of columnar cells associated with muscle fibers. In the distal region of the gland, the epithelium has spherical secretory cells with terminal apparatus and conducting canaliculi opening in the lumen through pores in the cuticle. Proteins were identified in the gland lumen, terminal apparatus, nucleus and cytoplasm of secretory cells. The histology of the R. neglectus gland is similar to that found in other species of this genus, but with variations in the shape and size of its distal region.

Keywords:
Chagas’ disease; oviposition; secretion; Triatominae

Resumo

Rhodnius neglectus é um triatomíneo silvestre, vetor do protozoário Trypanosoma cruzi, causador da doença de Chagas. Este inseto se alimenta do sangue de pequenos mamíferos, que é essencial para o seu crescimento e reprodução. As glândulas acessórias do sistema reprodutor feminino são importantes na reprodução de insetos, mas sua anatomia e histologia em R. neglectus são pouco conhecidas. O objetivo deste trabalho foi descrever a histologia e histoquímica da glândula acessória do aparelho reprodutor feminino de R. neglectus. O sistema reprodutor de cinco fêmeas de R. neglectus foi dissecado e as glândulas acessórias transferidas para solução fixadora de Zamboni, desidratadas em série crescente de etanol, embebidas em historesina, seccionadas com 2 µm de espessura, coradas com azul de toluidina para análise histológica ou submetidas ao teste de mercúrio-bromofenol para detecção de proteínas totais. O sistema reprodutor de R. neglectus tem uma glândula acessória tubular, sem ramificações, abrindo-se na região dorsal da vagina, sendo diferenciada em regiões proximal e distal. Na região proximal, a glândula é revestida internamente pela cutícula com uma camada de células colunares associadas a fibras musculares. Na região distal ocorrem células secretoras esféricas com aparato terminal e canalículos condutores que se abrem no lúmen da glândula através de poros na cutícula. O teste histoquímico revelou a presença de proteínas no lúmen da glândula e no aparato terminal, núcleo e citoplasma das células secretoras. A histologia da glândula de R. neglectus é semelhante à das espécies desse gênero, mas com variações na forma e no tamanho de sua região distal.

Palavras-chave:
doença de Chagas; oviposição; secreção; Triatominae

1. Introduction

Triatomines are wild hematophagous insects, among them, Rhodnius neglectus Lent, 1954 (Hemiptera: Reduviidae) vector of the protozoan Trypanosoma cruzi Chagas, 1909 (Kinetoplastida: Trypanosomatidae), which causes Chagas’ disease, feeds on the blood of small mammals (Gurgel-Gonçalves et al. 2003; Tartarotti et al., 2006). This insect is usually found on palm leaves and bird nests, in addition to reports in human homes (Rodrigues et al., 2009; Carvalho et al. 2014; Ramos et al. 2018). R. neglectus is endemic to the Brazilian Cerrado, but it is widely distributed in the Central, Northeast, Southeast and North regions of the country (Gurgel-Gonçalves and Cuba, 2009; Rodrigues et al., 2009; Ramos et al. 2018). The importance of this hematophagous insect for human health (Gurgel-Gonçalves et al., 2012) increases the need to knowledge its reproductive biology to contribute to the management of this Chagas’ disease vector.

The female reproductive tract of insects is formed by a pair of ovaries and lateral oviducts, a common oviduct, spermathecae, and accessory glands (Chapman, 2013). The accessory gland is usually unique in hematophagous Hemiptera, as reported for Triatoma lecticularia (Stal, 1859), Rhodnius brethesi Matta, 1919, Rhodnius nasutus Stal, 1859, and Rhodnius pictipes Stal, 1872 (Chiang et al., 2012; Monteiro et al., 2019). However, in other non-hematophagous hemipterans, including Cosmoclopius nigroannulatus (Stal, 1860) (Reduviidae) (Jahnke et al., 2006), Diaphorina citri Kuwayama, 1908 (Liviidae) (Dossi and Cônsoli., 2014), and Acyrthosiphon pisum (Harris, 1776) (Aphididae) (Wieczorek et al. al., 2019) the female reproductive tract has two accessory glands). In crickets, the accessory gland is characterized by a complex of tubules with numerous secretory branches, as reported for Acheta domesticus (Linnaeus, 1758), Gryllus bimaculatus De Geer, 1773, Gryllus assimilalis (Fabricius, 1775) and Teleogryllus commodus (Walker, 1869) (Orthoptera: Gryllidae) (Sturm, 2008; 2012).

The accessory gland of the female reproductive in insects has been associated with production of compounds the play role as a lubricant, improving the transport of fertilized eggs, protecting them against desiccation and predators, in addition to the attachment of eggs to the substrate at the time of oviposition (Chapman 2013). The antibacterial function of the secretion produced by the female accessory gland has been also reported in Diptera (Belardinelli et al., 2005). Proteins, lipids and carbohydrates are the main components found in the secretion released by the accessory glands (Lococo and Huebner, 1980b; Sturm 2002).

The accessory gland of the female reproductive tract of triatomines that show oviposition behavior without egg attachement to the substrate, such as Triatoma dimidiata (Latreille 1811) and Triatoma sordida (Stal 1859) (Hemiptera: Reduviidae), is reduced or absent (Chiang et al., 2012). On the other hand, this gland, in Rhodnius prolixus Stal, 1859 (Hemiptera: Reduviidae), is well-developed with a tubular shape, numerous spherical secretory cells and an excretory duct surrounded by muscle tissue (Lococo and Huebner, 1980b). This gland is known as cementum because it releases rich-protein secretion that spreads on the egg surface, strongly attaching it to the substrate (Lococo and Huebner, 1980b; Davey, 2007).

The accessory gland is highly specialized and fundamental in the reproductive process and success of insects, although it is not fully known in some species. The aim of this work was to describe the histology and histochemistry of the accessory gland of the female reproductive tract of R. neglectus.

2. Materials and Methods

2.1. Insects

Females of R. neglectus were obtained from the Department of Parasitology, Universidade Federal do Triângulo Mineiro (UFTM, 19°45’ S, 47°55’ W), Uberaba, state of Minas Gerais, Brazil. The colony was stablished from insects collected in Tocantinópolis, state of Tocantins, Brazil in 2014 and maintained in the laboratory at room temperature and natural illumination cycle. The females were transferred to the Cellular and Structural Biology Laboratory of the Universidade Federal de Viçosa Campus Rio Paranaíba in Rio Paranaíba, state of Minas Gerais, Brazil. The experiments carried out with animals were in accordance with the license 256, approved by the Animal Use Ethics Committee (CEUA) from UFTM (Carvalho-Costa et al., 2015). This insect was reared in cylindrical acrylic containers with pieces of folded cardboard to increase the surface area of refuge. The containers were sealed with a thin cotton fabric allowing the blood meal in chicken (Mendes, 2014).

2.2. Histology and histochemistry

Five R. neglectus females were cold anesthetized at 0 °C for five minutes and their reproductive tract dissected in 125 mM NaCl. The accessory gland was isolated and transferred to Zamboni’s fixative solution (Stefanini et al., 1967) for 24 hours at 4°C and then dehydrated in a graded ethanol series (70%, 80%, 90%, and 95%) for 15 minutes each. The accessory glands were embedded in Leica® historesin, sectioned at 2 μm thick in a Leica® rotatory microtome and stained in toluidine blue-borax. Some sections were submitted to the mercury bromophenol blue test to evidence total proteins (Bancroft and Gamble, 2008). These samples were analyzed in an Olympus® CX41 light microscope coupled with a Nikon D3100 camera.

3. Results

The female reproductive tract of R. neglectus consisted of a pair of ovaries and lateral oviducts opening in a common oviduct, spermathecae, and an accessory gland (Figure 1). The accessory gland of R. neglectus was connected to the dorsal region of the vagina, and was well-developed with an elongated, tubular-shaped, unbranched and coiled shape in the terminal region (Figure 1).

Figure 1
Female reproductive system of Rhodnius neglectus (Hemiptera: Reduviidae), consisting of two ovaries (Ov) with lateral oviducts (Lo) connected to the common oviduct (Co), spermathcae (Sp) and accessory glands (Ag).

The accessory gland, along its length, differs between the proximal and distal regions (Figure 1). In the proximal region, the epithelium was formed by a single layer of cubic cells with the lumen lined by a folded cuticular intima and the basal surface associated with thick longitudinal muscles (Figures 2A, D). The coiled distal region had the epithelium with spherical secretory cells with terminal apparatus containing a canaliculus that open in the lumen through pores in the cuticular intima (Figures 2B, C, D). In the terminal apparatus of the cells occurred the presence of secretion (Figure 2B). The secretory cells of the accessory gland of R. neglectus were spherical with a well-developed nucleus rich in decondensed chromatin and evident nucleolus (Figure 2C). Positive staining to protein was found in the region of secretory cells, terminal apparatus, canaliculi and in the lumen of the accessory gland of R. neglectus (Figures 3A, B).

Figure 2
Light micrographs of the accessory gland of the female reproductive tract of Rhodnius neglectus (Hemiptera: Reduviidae). (A) proximal (Pr) and distal regions (Dt) showing lumen (lu) covered by cuticle (ct); (B) Distal region showing secretory cells (sc) with secretion in the terminal apparatus (asterisk); (C) Secretory cells (sc) of the distal region with a nucleus (n) rich in decondensed chromatin and evident nucleolus (arrowheads) and the canaliculi (cc); (D) Proximal region showing cubic epithelium with thick cuticle (ct) and well-developed muscles (mu).

Figure 3
Light micrographs of the accessory gland of the female reproductive tract of Rhodnius neglectus (Hemiptera: Reduviidae) submitted to the mercury-bromophenol test. (A) Secretory cells (SC) showing positive reaction for proteins (black arrows) in the terminal apparatus (asterisks), nucleus (n), and canaliculi (cc); (B) Positive reaction for protein in the cuticle (ct) of the reservoir and in the canaliculi (cc).

4. Discussion

The anatomical features of the R. neglectus female reproductive tract, with a pair of lateral oviduct, common oviduct, spermathecae and an accessory gland, are common in insects, including Hemiptera representatives, such as Reduviidae Rhodnius montenegrensis da Rosa et al., 2012, Triatoma infestans (Klug, 1834) (Nascimento et al., 2017; 2019) and Liviidae D. citri (Dossi and Cônsoli., 2014). However, in Pentatomidae the accessory gland is absent (Lemos et al. 2005).

The female accessory gland of R. neglectus is well-developed due to the oviposition behavior of this insect, producing a large amount of secretion to attach the eggs to the substrate, as other Rhodnius spp. (Rabinovich et al., 2010; Chiang et al., 2012). However, this accessory gland is absent in triatomines without eggs that are attached to the substrate, including Nesotriato Nesotriatoma bruneri [= Nesotriatoma flavida (Neiva, 1911)] (Chiang et al., 2012; Tellez-Garcia et al., 2019). The tubular shape without ramifications of the accessory gland of the female reproductive tract of R. neglectus is similar to that reported in R. prolixus, called cement gland and common in this genus (Lococo and Huebner, 1980a; Chiang et al., 2012). However, with variations in the shape of the gland such as that in R. nasutus with accessory gland with an enlarged tube that bends over itself, and R. pictipes, in which the distal region of the gland is folded forming a globular structure (Chiang et al., 2012; Chiang and Chiang, 2017). The opening of the accessory gland, e.g, in the dorsal region of the vagina in R. neglectus varies among insects, as it is a structure involved in the reproduction process, as in D. citri opening in the common oviduct (Dossi and Cônsoli, 2014; Alba-Alejandre et al., 2020).

Differentiation along the length of the accessory gland in proximal and distal regions of R. neglectus with the proximal shorter and associated with muscles, may indicates a characteristic of transport of the secretion produced in the longer distal region. This gland type with different regions is common in the genus Rhodnius, but the distal region may be more dilated in a circular shape as in R. nasutus (Chiang et al. 2012; Lococo and Huebner, 1980a).

The gland duct lined by a folded cuticular intima, with cubic epithelium, and muscle fibers in the proximal region, indicates contractile activity of muscles, probably with pumping function to drive and control the release of secretion in the vaginal chamber, as suggested to occur in R. prolixus (Lococo and Huebner, 1980a). The release of secretion, by muscle contraction, has been suggested to coat eggs with secretion for oviposition (Chiang and O’Donnell, 2009; Sedra and Lange, 2014).

The presence of secretory cells with terminal apparatus and conducting canaliculus indicates that these cells are of class III, according to the classification of Noirot and Quennedey (1991). The terminal apparatus is a structure in the cytoplasm of the class III secretory cell and is usually located in the apical or basal region of the cell, function to collect the secretion produced in the cell (Stacconi and Romani, 2011; Souza et al., 2016; Pascini and Martins, 2017), confirming the secretion activity of the terminal apparatus by the presence of proteins rich secretion, observed after the histochemical test. Thus, the secretion produced is conducted through a conducting canaliculus associated with the terminal apparatus and released into the lumen through pores present in the cuticle that lines the central duct, such as in other Hemiptera (Lococo and Huebner, 1980a).

Secretory cells in the distal region of the accessory gland of R. neglectus female show the nucleus rich in decondensed chromatin and an evident nucleolus, indicating high metabolic activity. This suggests that their cytoplasm may be rich in endoplasmic reticulum, Golgi apparatus, mitochondria and secretory vesicles, that are related to the production of proteins present in the secretion (Stacconi and Romani, 2011; Sturm, 2012; Laghezza Masci et al., 2015).

Positive staining for protein in the secretory cells of the female accessory gland here observed, may be due to the cytoplasm and cell nucleus. Positive protein staining in the conducting canaliculi and lumen of the accessory gland of R. neglectus suggest that the secretion produced by the class III secretory cells is rich in protein, such as reported for R. prolixus, in which the released rich-protein secretion has adhesive function and acts on oviposition (Lococo and Huebner, 1980a). In addition to Rhodnius spp., the presence of proteins has been reported in the accessory gland of the female reproductive tract in other Hemiptera species as Acyrthosiphon pisum (Harris, 1776) and Acyrthosiphon svalbardicum Heikenheimo, 1968 (Hemiptera: Aphididae) (Wieczorek et al., 2019; 2020), and other insects such as T. commodus (Sturm, 2008) and Phlebotomus papatasi (Scopoli, 1786) (Diptera: Psychodidae) (Rosetto et al., 2003).

The accessory gland of the female reproductive tract of R. neglectus is similar to that of other species of this genus, with tubular morphology, without branches and with numerous secretory cells. The presence of muscle fibers, secretory cells with terminal apparatus, conducting canaliculi, and protein suggest the specialization in the synthesis, release and transport of rich-protein secretion.

Acknowledgements

We thank the Federal University of Triângulo Mineiro (UFTM) for providing specimens of Rhodnius neglectus for the study. We also thank Alessandra Santana Batista Toni for her support and reviews. This work was supported by Brazilian research agencies CNPq (303467/2018-5) and FAPEMIG (APQ-02367-18).

References

• ALBA-ALEJANDRE, I., ALBA-TERCEDOR, J. and HUNTER, W.B., 2020. Anatomical study of the female reproductive system and bacteriome of Diaphorina citri Kuwayama (Insecta: Hemiptera, Liviidae) using micro-computed tomography. Scientific Reports, vol. 10, no. 1, pp. 7161. http://dx.doi.org/10.1038/s41598-020-64132-y PMid:32346040.
  » http://dx.doi.org/10.1038/s41598-020-64132-y
• BANCROFT, J.D. and GAMBLE, M., 2008. Theory and practice of histological techniques 6th ed. London: Livingstone Churchill, 725 p.
• BELARDINELLI, M., FAUSTO, A.M., GUERRA, L., BUONOCORE, F., BONGIORNO, G., MAROLI, M. and MAZZINI, M., 2005. Lipase and antibacterial activities of a recombinant protein from the accessory glands of female Phlebotomus papatasi (Diptera: psychodidae). Annals of Tropical Medicine and Parasitology, vol. 99, no. 7, pp. 673-682. http://dx.doi.org/10.1179/136485905X51472 PMid:16212801.
  » http://dx.doi.org/10.1179/136485905X51472
• CARVALHO, D.B., ALMEIDA, C.B., ROCHA, C.S., GARDIM, S., MENDONÇA, V.J., RIBEIRO, A.R., ALVES, Z.C.P.V.T., RUELLAS, K.T., VEDOVELI, A. and ROSA, J.A., 2014. A novel association between Rhodnius neglectus and the Livistona australis palm tree in na urban center foreshadowing the risk of Chagas disease transmission by vectorial invasions in Monte Alto City, São Paulo, Brazil. Acta Tropica, vol. 130, pp. 35-38. http://dx.doi.org/10.1016/j.actatropica.2013.10.009 PMid:24145156.
  » http://dx.doi.org/10.1016/j.actatropica.2013.10.009
• CARVALHO-COSTA, T.M., MENDES, M.T., SILVA, M.V., COSTA, T.A., TIBURCIO, M.G.S., ANHÊ, C.B.M., RODRIGUES JUNIOR, V. and OLIVEIRA, C.J.F., 2015. Immunosuppressive effects of Amblyomma cajennense tick saliva on murine boné marrow-derived dendritic cells. Parasites & Vectors, vol. 8, no. 1, pp. 22. http://dx.doi.org/10.1186/s13071-015-0634-7 PMid:25586117.
  » http://dx.doi.org/10.1186/s13071-015-0634-7
• CHAPMAN, R.F., 2013. The Insects: structure and function Cambridge: Cambridge University Press. vol. 5, 929 p.
• CHIANG, R.G. and CHIANG, J.A., 2017. Reproductive physiology in the blood feeding insect, Rhodnius prolixus, from copulation to the control of egg production. Journal of Insect Physiology, vol. 97, pp. 27-37. http://dx.doi.org/10.1016/j.jinsphys.2016.06.001 PMid:27286950.
  » http://dx.doi.org/10.1016/j.jinsphys.2016.06.001
• CHIANG, R.G. and O’DONNELL, M.J., 2009. Functional anatomy of vagina muscles in the blood-feeding insect, Rhodnius prolixus. Arthropod Structure & Development, vol. 38, no. 6, pp. 499-507. http://dx.doi.org/10.1016/j.asd.2009.06.002 PMid:19573623.
  » http://dx.doi.org/10.1016/j.asd.2009.06.002
• CHIANG, R.G., CHIANG, J.A., SARQUIS, O. and LIMA, M.M., 2012. Morphology of reproductive accessory glands in eight species of blood-feeding Hemiptera (Hemiptera, Reduviidae) insect vectors of Chagas disease. Acta Tropica, vol. 122, no. 2, pp. 196-204. http://dx.doi.org/10.1016/j.actatropica.2012.01.011 PMid:22314031.
  » http://dx.doi.org/10.1016/j.actatropica.2012.01.011
• DAVEY, K., 2007. The interaction of feeding and mating in the hormonal control of egg production in Rhodnius prolixus. Journal of Insect Physiology, vol. 53, no. 3, pp. 208-215. http://dx.doi.org/10.1016/j.jinsphys.2006.10.002 PMid:17126364.
  » http://dx.doi.org/10.1016/j.jinsphys.2006.10.002
• DOSSI, F.C.A. and CÔNSOLI, F.L., 2014. Gross morphology and ultrastructure of the female reproductive sistem of Diaphorina citri (Hemiptera: liviidae). Zoologia, vol. 31, no. 2, pp. 162-169. http://dx.doi.org/10.1590/S1984-46702014000200007
  » http://dx.doi.org/10.1590/S1984-46702014000200007
• GURGEL-GONÇALVES, R. and CUBA, C.A.C., 2009. Predicting the Potential geographical distribution of Rhodnius neglectus (Hemiptera, Reduviidae) based on ecological niche modeling. Journal of Medical Entomology, vol. 46, no. 4, pp. 952-960. http://dx.doi.org/10.1603/033.046.0430 PMid:19645302.
  » http://dx.doi.org/10.1603/033.046.0430
• GURGEL-GONÇALVES, R., GALVÃO, C., COSTA, J. and PETERSON, A.T., 2012. Geographic distribution of Chagas disease vectors in Brazil based on ecological niche modeling. Journal of Tropical Medicine, vol. 2012, pp. 705326. http://dx.doi.org/10.1155/2012/705326 PMid:22523500.
  » http://dx.doi.org/10.1155/2012/705326
• GURGEL-GONÇALVES, R., PALMA, A.R.T., MENEZES, M.N.A., LEITE, R.N. and CUBA, C.A.C., 2003. Sampling Rhodnius neglectus in Mauritia flexuosa palm trees: a field study in the Brazilian savanna. Medical and Veterinary Entomology, vol. 17, no. 3, pp. 347-350. http://dx.doi.org/10.1046/j.1365-2915.2003.00448.x PMid:12941022.
  » http://dx.doi.org/10.1046/j.1365-2915.2003.00448.x
• JAHNKE, S.M., REDAELLI, L.R. and DIEFENBACH, L.M.G., 2006. Internal reproductive organs of Cosmoclopius nigroannulatus (Hemiptera: reduviidae). Brazilian Journal of Biology = Revista Brasileira de Biologia, vol. 66, no. 2A, pp. 509-512. http://dx.doi.org/10.1590/S1519-69842006000300017 PMid:16862306.
  » http://dx.doi.org/10.1590/S1519-69842006000300017
• LAGHEZZA MASCI, V., DI LUCA, M., GAMBELLINI, G., TADDEI, A.R., BELARDINELLI, M.C., GUERRA, L., MAZZINI, M. and FAUSTO, A.M., 2015. Reproductive biology in Anophelinae mosquitoes (Diptera, Culicidae): fine structure of the female accessory gland. Arthropod Structure & Development, vol. 44, no. 4, pp. 378-387. http://dx.doi.org/10.1016/j.asd.2015.04.003 PMid:25895726.
  » http://dx.doi.org/10.1016/j.asd.2015.04.003
• LEMOS, W.P., RAMALHO, F.S., SERRÃO, J.E. and ZANUNCIO, J.C., 2005. Morphology of female reproductive tract of the predator Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) fed on different diets. Brazilian Archives of Biology and Technology, vol. 48, no. 1, pp. 129-138. http://dx.doi.org/10.1590/S1516-89132005000100017
  » http://dx.doi.org/10.1590/S1516-89132005000100017
• LOCOCO, D. and HUEBNER, E., 1980a. The development of the female accessory gland in the insect Rhodnius prolixus. Tissue & Cell, vol. 12, no. 4, pp. 795-813. http://dx.doi.org/10.1016/0040-8166(80)90030-0 PMid:7010677.
  » http://dx.doi.org/10.1016/0040-8166(80)90030-0
• LOCOCO, D. and HUEBNER, E., 1980b. The ultrastructure of the female accessory gland, the cement gland, in the insect Rhodnius prolixus. Tissue & Cell, vol. 12, no. 3, pp. 557-580. http://dx.doi.org/10.1016/0040-8166(80)90045-2 PMid:7001676.
  » http://dx.doi.org/10.1016/0040-8166(80)90045-2
• MENDES, M.T., 2014. Avaliação do efeito da saliva de triatomíneos (Heteroptera: Reduviidae) sobre a biologia de células dendríticas murinas Uberaba: Universidade Federal do Triângulo Mineiro, 99 p. Dissertação de Mestrado em Medicina Tropical e Infectologia.
• MONTEIRO, M.F., LISBOA, L.C.O., CARVALHO-COSTA, T.M., NEVOA, J.C., OLIVEIRA, C.J.F., SERRÃO, J.E. and SOUZA, E.A., 2019. Morphology of the spermatheca of Triatoma lecticularia (Hemíptera, Reduviidae) (Stal, 1859). Brazilian Journal of Biology = Revista Brasileira de Biologia, vol. 79, no. 1, pp. 144-148. http://dx.doi.org/10.1590/1519-6984.180932 PMid:29590252.
  » http://dx.doi.org/10.1590/1519-6984.180932
• NASCIMENTO, J.D., CANEGUIM, B.H., PAULA, M.C., RIBEIRO, A.R., SASSO-CERRI, E. and ROSA, J.A., 2019. Spermathecae: morphofunctional features and correlation with fat bodies and trachea in six species of vectors of Chagas disease. Acta Tropica, vol. 197, pp. 105032. http://dx.doi.org/10.1016/j.actatropica.2019.05.023 PMid:31112712.
  » http://dx.doi.org/10.1016/j.actatropica.2019.05.023
• NASCIMENTO, J.D., RIBEIRO, A.R., ALMEIDA, L.A., OLIVEIRA, J., MENDONÇA, V.J., CILENSE, M. and ROSA, J.A., 2017. Morphology of the spermathecae of twelve species of Triatominae (Hemiptera, Reduviidae) vectors of Chagas disease. Acta Tropica, vol. 176, pp. 440-445. http://dx.doi.org/10.1016/j.actatropica.2017.08.024 PMid:28859957.
  » http://dx.doi.org/10.1016/j.actatropica.2017.08.024
• NOIROT, C. and QUENNEDEY, A., 1991. Glands, gland cells, glandular units: some comments on terminology and classification. Annales de la Société Entomologique de France, vol. 27, pp. 123-128.
• PASCINI, T.V. and MARTINS, G.F., 2017. The insect spermatheca: an overview. Zoology, vol. 121, pp. 56-71. http://dx.doi.org/10.1016/j.zool.2016.12.001 PMid:28089345.
  » http://dx.doi.org/10.1016/j.zool.2016.12.001
• RABINOVICH, J.E., NIEVES, E.L. and CHAVES, L.F., 2010. Age‐specific mortality analysis of the dry forest kissing bug, Rhodnius neglectus. Entomologia Experimentalis et Applicata, vol. 135, no. 3, pp. 252-262. http://dx.doi.org/10.1111/j.1570-7458.2010.00986.x
  » http://dx.doi.org/10.1111/j.1570-7458.2010.00986.x
• RAMOS, L.J., CASTRO, G.V.D.S., SOUZA, J.L., OLIVEIRA, J., ROSA, J.A., CAMARGO, L.M.A., CUNHA, R.M. and MENEGUETTI, D.U.D.O., 2018. First report of Rhodnius neglectus (Hemiptera, Reduviidae, Triatominae) from the state of Acre, Brazil, and the Brazilian Western Amazon Region. Revista da Sociedade Brasileira de Medicina Tropical, vol. 51, no. 2, pp. 212-214. http://dx.doi.org/10.1590/0037-8682-0320-2017 PMid:29768556.
  » http://dx.doi.org/10.1590/0037-8682-0320-2017
• RODRIGUES, V.L.C.C., SILVA, R.A., WANDERLEY, D.M.V., CARVALHO, M.E. and PAULIQUEVIS JUNIOR, C., 2009. Detecção de triatomíneos da espécie Rhodnius neglectus em área urbana de municípios da região de Araçatuba. Boletim Epidemiológico Paulista, vol. 6, no. 63, pp. 20-23.
• ROSA, J.A., ROCHA, C.S., GARDIM, S., PINTO, M.C., MENDONÇA, V.J., FERREIRA FILHO, J.C.R., CARVALHO, E.O.C.D., CAMARGO, L.M.A., OLIVEIRA, J.D., NASCIMENTO, J.D., CILENSE, M. and ALMEIDA, C.E., 2012. Description of Rhodnius montenegrensis n. sp. (Hemiptera: Reduviidae: Triatominae) from the state of Rondônia, Brazil. Zootaxa, vol. 3478, no. 1, pp. 62-76. http://dx.doi.org/10.11646/zootaxa.3478.1.8
  » http://dx.doi.org/10.11646/zootaxa.3478.1.8
• ROSETTO, M., BELARDINELLI, M., FAUSTO, A.M., MARCHINI, D., BONGIORNO, G., MAROLI, M. and MAZZINI, M., 2003. A mammalian‐like lipase gene is expressed in the female reproductive accessory glands of the sand fly Phlebotomus papatasi (Diptera, Psychodidae). Insect Molecular Biology, vol. 12, no. 5, pp. 501-508. http://dx.doi.org/10.1046/j.1365-2583.2003.00436.x PMid:12974955.
  » http://dx.doi.org/10.1046/j.1365-2583.2003.00436.x
• SEDRA, L. and LANGE, A.B., 2014. The female reproductive system of the kissing bug, Rhodnius prolixus: arrangements of muscles, distribution and myoactivity of two endogenous FMRFamide-like peptides. Peptides, vol. 53, pp. 140-147. http://dx.doi.org/10.1016/j.peptides.2013.04.003 PMid:23598080.
  » http://dx.doi.org/10.1016/j.peptides.2013.04.003
• SOUZA, E.A., LISBOA, L.C.O., ARAÚJO, V.A. and SERRÃO, J.E., 2016. Morphology of the spermathecae of Leptoglossus zonatus (Heteroptera: coreidae). Annals of the Entomological Society of America, vol. 109, no. 1, pp. 106-111. http://dx.doi.org/10.1093/aesa/sav097
  » http://dx.doi.org/10.1093/aesa/sav097
• STACCONI, M.V.R. and ROMANI, R., 2011. Ultrastructural and functional aspects of the spermatheca in the American Harlequin Bug, Murgantia histrionica (Hemiptera: pentatomidae). Neotropical Entomology, vol. 40, no. 2, pp. 222-230. http://dx.doi.org/10.1590/S1519-566X2011000200011 PMid:21584404.
  » http://dx.doi.org/10.1590/S1519-566X2011000200011
• STEFANINI, M., MARTINO, C. and ZAMBONI, L., 1967. Fixation of ejaculated spermatozoa for eléctron miscroscopy. Nature, vol. 216, no. 5111, pp. 173-174. http://dx.doi.org/10.1038/216173a0 PMid:4862079.
  » http://dx.doi.org/10.1038/216173a0
• STURM, R., 2002. Morphology and ultrastructure of the female accessory sex glands in various crickets (Orthoptera, Saltatoria, Gryllidae). Deutsche Entomologische Zeitschrift, vol. 49, no. 2, pp. 185-195. http://dx.doi.org/10.1002/mmnd.20020490203
  » http://dx.doi.org/10.1002/mmnd.20020490203
• STURM, R., 2008. Morphology and histology of the ductus receptaculi and accessory glands in the reproductive tract of the female cricket, Teleogryllus commodus. Journal of Insect Science, vol. 8, no. 1, pp. 35. https://doi.org/10.1673/031.008.3501
  » https://doi.org/10.1673/031.008.3501
• STURM, R., 2012. Morphology and ultrastructure of the accessory glands in the female genital tract of the house cricket, Acheta domesticus. Journal of Insect Science, vol. 12, pp. 99. http://dx.doi.org/10.1673/031.012.9901 PMid:23425229.
  » http://dx.doi.org/10.1673/031.012.9901
• TARTAROTTI, E., AZEREDO-OLIVEIRA, M.T.V. and CERON, C.R., 2006. Phylogenetic approach to the study of triatomines (Triatominae, Heteroptera). Brazilian Journal of Biology = Revista Brasileira de Biologia, vol. 66, no. 2B, pp. 703-708. http://dx.doi.org/10.1590/S1519-69842006000400014 PMid:16906302.
  » http://dx.doi.org/10.1590/S1519-69842006000400014
• TELLEZ-GARCIA, A.A., BELLO-BEDOY, R., ENRÍQUEZ-VARA, J.N., CÓRDOBA-AGUILAR, A. and GUTIÉRREZ-CABRERA, A.E., 2019. Genital morphology and copulatory behavior in triatomine bugs (Reduviidae: triatominae). Arthropod Structure & Development, vol. 49, pp. 103-118. http://dx.doi.org/10.1016/j.asd.2018.11.012 PMid:30529710.
  » http://dx.doi.org/10.1016/j.asd.2018.11.012
• WIECZOREK, K., CHLOND, D., JUNKIERT, L. and ŚWIATEK, P., 2020. Structure of the reproductive system of the sexual generation of the endemic Arctic species Acyrthosiphon svalbardicum and its temperate counterpart Acyrthosiphon pisum (Hemiptera, Aphididae). Biology of Reproduction, vol. 103, no. 5, pp. 1043-1053. http://dx.doi.org/10.1093/biolre/ioaa147 PMid:33145591.
  » http://dx.doi.org/10.1093/biolre/ioaa147
• WIECZOREK, K., KANTURSKI, M., SEMPRUCH, C. and ŚWIATEK, P., 2019. The reproductive system of the male and oviparous female of a model organism - the pea aphid, Acyrthosiphon pisum (Hemiptera, Aphididae). PeerJ, vol. 7, e7573. http://dx.doi.org/10.7717/peerj.7573 PMid:31534847.
  » http://dx.doi.org/10.7717/peerj.7573

Publication Dates

• Publication in this collection
  15 May 2023
• Date of issue
  2023

History

• Received
  09 Feb 2023
• Accepted
  10 Apr 2023