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Biota Neotropica

On-line version ISSN 1676-0611

Biota Neotrop. vol.2 no.2 Campinas  2002

https://doi.org/10.1590/S1676-06032002000200011 

ARTIGOS

 

Estrutura genética de populações naturais de Cryptocarya aschersoniana Mez (Lauraceae) através de marcadores isoenzimáticos

 

Genetic structure of natural populations of Cryptocarya aschersoniana Mez (Lauraceae) through isozyme markers

 

 

Pedro Luís Rodrigues de MoraesI, II, *; Maria Teresa Vitral de Carvalho DerbyshireI, II

IBolsa de Pós-Doutoramento a P.L.R. Moraes (FAPESP 99/05004-5), auxílio à pesquisa (BIOTA/FAPESP 99/05818-2)
IILaboratório de Melhoramento de Plantas, CENA/USP, Caixa Postal 96, 13400-970, Piracicaba, SP, Brasil

 

 


ABSTRACT

Through the analysis of 39 polymorphic allozyme loci, allele frequencies were estimated from 267 individuals of 12 natural populations of Cryptocarya aschersoniana native to "Planalto forests" of the state of São Paulo and south of Minas Gerais, Brazil. Estimates of Wright's F statistics were computed through the analysis of variance for obtaining unbiased estimation of corresponding parameters F=FIT, θ P =FST and f=FIS. Average values of F^ were 0.552 < 0.415 < 0.275; those of θ^P were 0.395 < 0.335 < 0.279; and those of f^ were 0.292 < 0.119 < -0.039. These results indicated that individuals within populations might be panmitic, and that the diversity among populations was fairly high, being superior to what would be expected for groups of plants having a full-sib family structure. From estimates of θ^P obtained for populations taken two at a time, the model of isolation by distance was employed and it has shown to be inadequate for explaining the divergence found among populations. The estimated gene flow of 0.4 migrants per generation corroborated the pronounced populational differentiation. Due to negligible f^ @ F^IS values found, the variance effective size for each population was equivalent to its sampling number. Under a metapopulation context, considering the 12 populations sampled for the species, the effective population size was 15.4 individuals (5.77%) for the total sampled, indicating that sampling of different populations should be an important strategy for their management.

Key words: allozymes, Lauraceae, genetic structure, Neotropics, Cryptocarya aschersoniana, Planalto forest, Brazil


RESUMO

Pela análise de 39 locos isoenzimáticos polimórficos, estimaram-se as freqüências alélicas referentes a 267 indivíduos de 12 populações naturais de Cryptocarya aschersoniana provenientes de Florestas de Planalto do estado de São Paulo e sul de Minas Gerais, Brasil. Foram obtidas estimativas das estatísticas F de Wright pelo método da análise da variância para estimação não viesada dos parâmetros correspondentes F=FIT, θ P =FST e f=FIS. Os valores médios obtidos de F^ resultaram em 0,552 < 0,415 < 0,275; os de θ^P foram 0,395 < 0,335 < 0,279; e os de f^ sendo 0,292 < 0,119 < -0,039. Esses resultados indicaram que os indivíduos dentro das populações devem ser panmíticos e que a diversidade entre populações foi bastante alta, sendo superior à que poderia se esperar para famílias com estruturação de irmãos-germanos. Calculando-se θ^P com as populações tomadas duas a duas, testou-se o modelo de isolamento pela distância que se mostrou inadequado para explicar a divergência encontrada entre as populações. O fluxo gênico estimado de 0,4 indivíduos por geração corroborou a pronunciada diferenciação populacional. Devido aos valores insignificantes encontrados de f^ @ F^IS, o tamanho efetivo de variância de cada população foi equivalente ao número de indivíduos amostrados. Sob um contexto metapopulacional, considerando-se as 12 populações amostradas para a espécie, o tamanho efetivo populacional foi de 15,4 indivíduos (5,77%) para o total amostrado, indicando que a amostragem de diferentes populações deve ser uma estratégia importante para seu manejo.

Palavras-chave:alozimas, Lauraceae, estrutura genética, Neotrópico, Cryptocarya aschersoniana, Mata de Planalto, Brasil


 

 

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5. Agradecimentos

Ao Instituto de Botânica, Prefeitura Municipal de Campinas, Fundação José Pedro de Oliveira, Sr. José Carlos Reis de Magalhães, Sr. Djalma Brasil Zabeu, pelas respectivas autorizações de coleta na Fazenda Campininha, nos "Bosques" do município, na Mata de Santa Genebra, na Fazenda Barreiro Rico, e na Fazenda Palmital. Ao Marco Aurélio Nalon pela feitura do mapa de localização das áreas estudadas. Ao Angelo Gilberto Manzatto e Antonio Carlos Scutti, pela logística e auxílio nas coletas na Fazenda São José e na região do sul de Minas Gerais, respectivamente. Aos estagiários Mariana Campanholi Daher e Fernando Henrique Romano, pelo auxílio nas análises laboratoriais. Às críticas e sugestões de dois referees anônimos que em muito contribuíram para o aprimoramento do manuscrito.

 

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Recebido em 09/09/2002
Revisado em 30/10/2002
Publicado em 29/11/2002

 

 

* Autor para correspondência: E-mail: plmoraes@cena.usp.br

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