List of Odonates from the Floresta Nacional de São Francisco de Paula (FLONA - SFP), with two new distribution records for Rio Grande do Sul, Brazil

Lista de Odonata da Floresta Nacional de São Francisco de Paula (FLONA - SFP), com dois novos registros de distribuição para o Rio Grande do Sul, Brasil

Abstract

A survey of Odonata was carried out in the National Forest FLONA - SFP, Northeastern region of the Rio Grande do Sul state, Brazil. This conservation unit is mainly covered by Mixed Ombrophilous Forest (MOF), a subtype of Atlantic Forest biome, being also areas covered in planted Pinus, planted Araucaria and open fields. Our sampling efforts were conducted in thirty aquatic environments inside this reserve during the period between January 2014 and November 2014. The sampling sites were selected randomly, comprehending lakes, bogs, small streams and river sections, all inserted in the four vegetation types occurring in the reserve. Fortysix species of Odonata were collected and grouped into 23 genera and seven families. The dominant families were Coenagrionidae (32%), Libellulidae (32%), Aeshnidae (12%), and, Calopterygidae and Lestidae (9%). As expected, the findings revealed the presence of a highly diverse Odonate assemblage, mainly represented by generalist species in the most human disturbed sectors (Pinus and Open fields) and some specialist species in the pristine forest. Two species were registered for the first time in the state of Rio Grande do Sul, Brazil: Libellula herculea Karsch, 1889 (Libellulidae) and Heteragrion luizfelipei Machado, 2006 (Heteragrionidae).

Keywords
Ecology; Odonata; inventory; Atlantic Forest; distribution

Resumo

Uma pesquisa de Odonatas foi desenvolvida na Floresta Nacional de São Francisco de Paula (FLONA - SFP), Rio Grande do Sul, Brasil. Esta unidade de conservação é, em grande parte, coberta por Floresta Ombrófila Mista, um subtipo de floresta do Bioma Mata Atlântica, sendo também áreas cobertas com plantação de Pinus, Araucaria, além de áreas de campo aberto. Nossos esforços de coleta abrangeram 30 ambientes aquáticos dentro da reserva, no período de Janeiro de 2014 e Novembro de 2014. Os locais de coleta foram selecionados aleatoriamente, compreendendo lagos, banhados, pequenos arroios e seções de rio, todos inseridos nos quatro tipos de vegetação ocorrentes na reserva. Foram coletadas 46 espécies de Odonata, agrupadas em 23 gêneros e sete famílias. As famílias dominantes foram Coenagrionidae (32%), Libellulidae (32%), Aeshnidae (12%), e, Calopterygidae e Lestidae (9%). Como esperado, os resultados revelaram a presença de uma alta diversidade de Odonata, em sua maioria generalistas, encontrados nos setores mais impactados pelo homem (Pinus e Campos abertos) e algumas espécies especialistas na floresta primária. Duas espécies foram registradas pela primeira vez no estado do Rio Grande do Sul, Brasil: Libellula herculea Karsch, 1889 (Libellulidae) e Heteragrion luizfelipei Machado, 2006 (Heteragrionidae).

Palavras-chave
Ecologia; Odonata; inventário; Floresta Atlântica; distribuição

Introduction

A big number of studies to describe the diverse fauna and flora in the Neotropics are already being published or under way. Even with so intense efforts, we are still far from the knowledge levels achieved in Europe and North America, or the northern hemisphere in general. In this part of the world, we even still lack reliable estimates of how many species occurs (May 1998, Scotland & Wortley 2003). Every day the information on the diversity of species becomes more relevant, as well as the knowledge on the species distribution and richness, which can provide several tools to put focus on human impact on these environments (Lewis 2006). To prioritize among areas under consideration for conservation, biologists and decision makers need concise information on species diversity, especially in threatened habitats like the Atlantic Forest (Kerr et al. 2000).

The Brazilian Odonata are still poorly described, despite a few publications with ecological approach, species lists are known only from São Paulo (Costa et al. 2000); Espírito Santo (Costa & Oldrini 2005); Minas Gerais (Souza et al. 2013) for Mata do Baú specifically; and Mato Grosso (Calvão et al. 2014) this last one being restricted only to a small region of the state. The most recent Odonata inventory for the state of Rio Grande do Sul is Renner et al. (2015), for the central region, and Kittel & Engels (2014), from a nature reserve (Pró-mata/PUCRS), with sampling performed only in open areas in forest surroundings and restricted to the Zygoptera suborder.

In this paper, we aim to improve the knowledge of the Odonata of the northeastern region of the Rio Grande do Sul state, acquiring a general overview of the species distribution over several types of aquatic environments distributed in Atlantic Forest at relatively high altitudes. As consequence we also expanded species distributions for this state.

Material and Methods

1. Study area

All the sampling areas are located within the National Forest FLONA - SFP, at the São Francisco de Paula municipality (Figure 1). This reserve is a National Conservation Unit, administered by de ICMBio (Instituto Chico Mendes de Conservação da Biodiversidade) together with the Brazilian Environment Ministry (Ministério do Meio Ambiente). These units are part of a sustainability plan, being so, part of the total areas are cultivated with planted forest: Araucaria angustifolia (Bertol.) Kuntze and Pinus elliottii Engelm., producing commercial wood for economical purposes.

Figure 1
Brazilian map with the insets of the state of Rio Grande do Sul (RS); São Francisco de Paula municipality (light gray); and the complete map of National Forest FLONA - SFP, yellow dots representing the sampling sites in the reserve.

The climate in the region is classified as Cfb in the Koppen system, Mesothermic Humid, Temperate Subtropical, with well defined seasons, mean temperatures varying in between 12°C and 16°C, altitudes reaching from 900 m to 1,100 m ASL, and precipitation means above 2,000 mm annually (INPE 2014). The reserve area (1,572 ha) is mainly (720 ha) covered in pristine Mixed Ombrophilous Forest (MOF), a subtype of Atlantic Forest, some areas are covered in planted Araucaria (AR), planted Pinus (PN) and Open fields (OP), these areas being altered since the 1950's decade. In each of these four vegetation types, three types of aquatic environments were selected: rivers (R), lakes (L) and swamps (S) to provide an overall view of the species occurring in the area.

2. Data collection

In total 30 aquatic environments (Figure 1) were sampled: MOF (n = 12), AR (n = 8), PN (n = 6) and OP (n = 4). The aquatic environments were divided in 3 types: rivers (R) n = 14, lakes (L) n = 7 and swamps (S) n = 9. We sampled for dragonflies during the year of 2014, all the sampling sites were visited three times per year, once per season: summer (2 - 11 January); autumn (10 - 18 May); spring (2 - 11 November); excluding the winter season due to the lack of activity of adult Odonata in the low temperatures prevailing. Our sampling efforts were concentrated on adults of Odonata, since the larvae can present great difficulties to the determination work, as only scarce information is available for this region (Garrison et al. 2006). The sampling method used handhold insect nets, performed by a team of two persons, in sunny days, during the peak time of Odonata activities (between 09:00 h to 16:00 h), which is an adaptation of Renner et al. (2015). The collection authorization process was issued by IBAMA, through SISBio system under the number 38928-1.

Species richness was determined by the absolute number of collected specimens. Collection efforts and the number of sampled species were based on the richness percentage estimated by the mean of non-parametric estimator building the collector's curve by Jack1 and Chao1 to estimate the actual number of species. The indexes Jackknife1 and Chao1 were calculated using the Software Estimates (Colwell 2009), with 1000 repetitions. Our sampling efforts were also validated using the estimation of total richness according to Smith & van Belle (1984).

All specimens collected were immediately preserved in 96% ethanol and deposited in the MCNU, afterwards determined in the Evolution and Ecology Laboratory according to Garrison et al. (2006, 2010), Heckman (2006, 2010), Lencioni (2006) and original species descriptions. For the systematics classification, we followed Dijkstra et al. (2014).

Results

1. Species list

We found 46 species, belonging to 23 genera in seven families, collected in the 30 aquatic environments inside the National Forest FLONA - SFP, except two, which are located in the immediate surroundings (main access road). This is a relatively large number of species, reflecting the diversity of sampling sites, which includes three types of aquatic systems, and four types of vegetation. In total 454 specimens were collected. The estimated number of species through non-parametric indexes (Jacknife1 and Chao1) are shown in Figure 2, the total estimated number of species through Smith & van Belle (1984) was 55,67, showing we reached 82% (n = 46) of the total estimated number of species on the three sampling occasions.

Figure 2
Number of species of Odonata collected (sobs) on the 30 sampling sites in relation to the number of species expected by Jacknife1 and Chao1 estimators in the National Forest FLONA - SFP, state of Rio Grande do Sul (RS), Brazil.

The Odonate list records from the National Forest FLONA - SFP is presented in Table 1, each species accompanied by the acronym of the vegetation type and aquatic environments with occurrence and collection/voucher ID number.

Table 1
Inventory list of Odonata species from the National Forest FLONA - SFP.

Suborder	Family	Species	Vegetation type	Env. type	Collection ID
Zygoptera	Calopterygidae	Hetaerina longipes Hagen in Selys, 1853	MOF, AR, OP	R, S	ZAUMCN928
		Hetaerina rosea Selys, 1853	MOF, PN	R, S	ZAUMCN929
		Mnesarete borchgravii (Selys, 1869)	MOF, AR	R	ZAUMCN930
		Mnesarete pruinosa (Hagen in Selys, 1853)	MOF, OP	R	ZAUMCN931
	Coenagrionidae	Acanthagrion gracile (Rambur, 1842)	OP	L	ZAUMCN932
		Acanthagrion lancea Selys, 1876	AR, PN, OP	L, S	ZAUMCN933
		Acanthagrion sp.	MOF, PN	R, L	ZAUMCN934
		Argentagrion ambiguum (Ris, 1904)	AR	S	ZAUMCN935
		Argia indocilis Navás, 1934 (j. syn. A. croceipennis)	MOF, AR, OP	R	ZAUMCN936
		Argia sp.	MOF	R	ZAUMCN937
		Homeoura chelifera (Selys, 1876)	AR, PN, OP	L	ZAUMCN938
		Ischnura capreolus (Hagen, 1861)	AR, PN, OP	L	ZAUMCN939
		Ischnura fluviatilis Selys, 1876	AR, PN, OP	L	ZAUMCN940
		Oxyagrion hempeli Calvert, 1909	OP	R	ZAUMCN942
		Oxyagrion microstigma Selys, 1876	PN, OP	R, L	ZAUMCN943
		Oxyagrion terminale Selys, 1876	MOF, AR, PN, OP	R, L, S	ZAUMCN944
		Oxyagrion sp.	MOF, AR, PN, OP	R, L, S	ZAUMCN945
		Peristicta gauchae Santos, 1968	OP	R	ZAUMCN946
		Telebasis theodori (Navás, 1934)	PN, OP	L	ZAUMCN947
	Heteragrionidae	Heteragrion luizfelipei Machado, 2006	MOF	R	ZAUMCN948
	Lestidae	Lestes auritus Hagen in Selys, 1862	PN, OP	L, S	ZAUMCN949
		Lestes bipupillatus Calvert, 1909	OP	S	ZAUMCN950
		Lestes pictus Hagen in Selys, 1862	PN	L	ZAUMCN952
		Lestes undulatus Say, 1840	PN, OP	L	ZAUMCN951
Anisoptera	Aeshnidae	Castoraeschna sp.	AR, PN	S	ZAUMCN953
		Limnetron sp.	MOF	R, S	ZAUMCN954
		Rhionaeschna bonariensis Rambur, 1842	MOF, AR, PN, OP	R, L, S	ZAUMCN956
		Rhionaeschna brasiliensis Ellenrieder and Costa, 2002	MOF, PN	R, L, S	ZAUMCN955
		Rhionaeschna planaltica (Calvert, 1952)	MOF, AR, PN, OP	R, L, S	ZAUMCN957
	Gomphidae	Phyllogomphoides regularis (Selys, 1873)	MOF	R	ZAUMCN958
		Progomphus gracilis Hagen in Selys, 1854	MOF, AR, OP	R	ZAUMCN959
	Libellulidae	Dasythemis mincki mincki (Karsh, 1890)	AR	S	ZAUMCN960
		Erythrodiplax atroterminata Ris, 1911	MOF	R	ZAUMCN961
		Erythrodiplax fusca (Rambur, 1842)	AR, PN, OP	L	ZAUMCN963
		Erythrodiplax hyalina Förster, 1907	MOF, AR, PN, OP	R, L, S	ZAUMCN964
		Erythrodiplax media Borror, 1942	MOF, AR, PN, OP	R, L, S	ZAUMCN965
		Erythrodiplax sp. (1)	MOF, AR, PN, OP	R, L, S	ZAUMCN962
		Erythrodiplax sp. (2)	OP	L	ZAUMCN966
		Libellula sp.	MOF, PN	S	ZAUMCN967
		Libellula herculea Karsch, 1889	PN	S	ZAUMCN968
		Macrothemis marmorata Hagen, 1868	AR	S	ZAUMCN969
		Micrathyria artemis Ris, 1911	MOF	R	ZAUMCN970
		Oligoclada laetitia Ris, 1911	AR, PN	L, S	ZAUMCN971
		Orthemis discolor (Burmeister, 1839)	PN	S	ZAUMCN972
		Perithemis icteroptera (Selys in Sagra, 1857)	PN	L	ZAUMCN973
		Perithemis mooma Kirby, 1889	PN	L	ZAUMCN974

The families with the higher number of species were Coenagrionidae (32%), Libellulidae (32%), Aeshindae (12%), Calopterygidae and Lestidae (both 9%), as shown in the Table 2. The most common genera were Erythrodiplax and Oxyagrion, represented by six and four species, respectively. Erythrodiplax was the most abundant genus, occurring virtually in all sampling sites. The rarest species found with only one occurrence and in only one season were Macrothemis marmorata, Dasythemys m. mincki and Peristicta gauchae.

Table 2
Number of Odonata species collected per family in the National Forest (FLONA - SFP).

FAMILY	Nº	%
ZYGOPTERA
Coenagrionidae	15	32
Lestidae	4	9
Calopterygidae	4	9
Heteragrionidae	1	2
ANISOPTERA
Libellulidae	15	32
Aeshnidae	5	12
Gomphidae	2	4
TOTAL	46	100%

2. New records

We found two new records of odonate species for Rio Grande do Sul, one belonging to Libellulidae: Libellula herculea Karsch, 1889; and one belonging to Heteragrionidae: Heteragrion luizfelipeiMachado, 2006.

2.1. Libellula herculea Karsch, 1889

Libellula is a Holarctic genus, which occurs also in Europe and Asia, in the New World its species are most diverse in North America: from Alaska and N Canada and to N Argentina in South America. The only species known to be widespread in South America is Libellula herculea (Garrison et al. 2006). According to Carle & Kjer (2002) this genus is well diagnosed and is considered a sister group of Orthemis, however an undescribed species is known from Brazil (Garrison et al. 2006). The two species belonging to the genus Libellula recorded here are: Libellula herculea and Libellula sp. both distributed widely in North, Central America and northern regions of South America, and with our records expanding their distribution southwards. The current distribution records were derived from De Marmels (1982) and Heckman (2006). According to Garrison (2006) the genus distribution ranges from North America to the Paraná state in Brazil, around 250 km northwards of our sampling site. Regarding Libellula herculea, the records from Brazil are from Marambaia, Ilha Grande and Itatiaia in the state of Rio de Janeiro (Santos 1970). Our specimens of Libellula herculea were found only in a permanent, highly vegetated swamp, inside the Pinus (PN) plantation (Figure 3a).

Figure 3
a) Main features of Libellula herculea: Male cercus (lateral view); FW base, arculus sectors separated; Genital fossa, ventral view. b) Heteragrion luizfelipei, adult male.

2.2. Heteragrion luizfelipeiMachado, 2006

This species (Figure 3b) have its current distribution restricted to southern and southeastern Brazil, from São Paulo (Machado, 2006), and Paraná to its southern limit in the Santa Catarina state as reported by Lencioni (2013). Here we expand the distribution around 250 km southwards in the Rio Grande do Sul state. No further reported records could be found for this species. Possibly the lack of sampling efforts in the Atlantic coastal zones covered by the Atlantic Forest biome can explain why no sighting of this species have taken place in this region, as it is known as a typical forest genus (Loiola & De Marco 2011). The specimens were collected in the proximities of a slow flowing stream (ca. 50 - 100 cm wide), with boulder/gravel bottom, mostly shaded by the dense pristine Atlantic Forest (MOF).

Most of the biodiversity in the Atlantic Forest still unknown, especially so in the MOF subtype (Southern Brazil), and more studies are needed as shown by the discovery of these two new records. The order Odonata in the Neotropics in general is in need of many taxonomic revisions which will only be made possible through sampling and inventory studies as this one. We also strongly recommend the creation of more protected areas in the region, since it is considered a "hotspot" for biodiversity.

Discussion

The size of regional species pools for dragonflies has only been studied in certain regions of the world. In temperate regions of northern Europe, specifically southern and central Sweden, several studies have reported a regional species pool of between 24 and 30 (Wittwer et al. 2010; Flenner & Sahlén 2008). According to studies from Brazil, Pires et al. (2013) found 30 genera of dragonfly larvae in the Jacuí River Basin; De Marco et al. (2014), listed a species pool of 56, in central Brazi; Monteiro et al. (2013), noted 32 species in Manaus, Amazonas; Anjos-Santos & Costa (2006), registered 77 species, from Rio de Janeiro; and Souza et al. (2013) recorded 47 in Mata do Baú, Minas Gerais. Thus, 46 species fit well into the general pattern for a Subtropical zone, although there may be some additional rare species occurring in the region, the estimated indexes indicate that the size of the species pool is correctly estimated although up to ten more species might occur.

The big numbers of Libellulids occurring in the PN and OP (the most human impacted areas) consists to large part by widespread generalists, a fact that can be attributed to the mosaic of forest and open areas, which by its time favors the occupation by the fast and agile flying dragonflies corroborating the findings from Machado (2001).

There are clear relations between environmental factors (biotic and abiotic) and species composition, these factors acting as determinants of presence and absence of some species due to ecological and physical restrictions (Paulson 2006, Juen et al. 2007). In most preserved areas or bigger forested areas it is expected to find a bigger number of Calopterygids and Heteragrionids, which have more environmental restrictions and ecological needs to occur (Carvalho et al. 2013).

Despite that the occurrence of rare species was mostly restricted to the MOF and AR sectors, the finding of a good number of Coenagrionids in the other sectors (PN, OP) can also mirror good conditions, since some genera have also ecological restrictions related to aquatic vegetation and water quality (e.g. Homeoura, Argia, Oxyagrion) as stated by Garrison et al. (2010).

Regarding the occurrence indicator species, we found a selection which could fit into an indicator of general species richness for fragmented Atlantic forest biome, following Renner et al. (2015), as for example Ischnura capreolus and Homeoura chelifera, occurring only in the non-natural environments (AR, PN and OP). No endangered species were found, since most of the records are now attributed to "Least concern" or "Data deficient", according to the IUCN.

The Atlantic forest is one of the most endangered ecosystems in South America, and in this case, more specifically, the Mixed Ombrophilous Forest, one of the less known forest subtypes was explored. For these environments, species inventories can produce a decisive tool for the management actions in order to keep and restore forests and natural resources. This survey made a deepened approach of the poorly known Odonata from Southern Brazil, showing that a great diversity is still occurring despite the human impacted habitats included in this study. This knowledge is useful in providing support data for future conservation measurements in the studied landscape.

Acknowledgements

This project was partially supported by Brazilian government agencies, CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) through a Masters fellowship to the first author, FAPERGS (Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul) and Centro Universitário Univates, for additional funding and logistic support; IBAMA for the collection permit; F.A.A. Lencioni, who helped with the identification of the Heteragrionids; Mrs. Úrsula Arend (MCNU), for the labelling of our collection; and to the FLONA staff, who kindly provided our accommodations during the field works.

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Publication Dates

• Publication in this collection
  2016

History

• Received
  10 Nov 2015
• Reviewed
  14 June 2016
• Accepted
  10 July 2016