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Jornal Vascular Brasileiro

versão impressa ISSN 1677-5449versão On-line ISSN 1677-7301

J. vasc. bras. v.6 n.2 Porto Alegre jun. 2007 



Assessment of induced healing by porous cellulose membrane after complete removal of a skin segment from the back of rats



Nelson Yaguishita

Member, SBACV




INTRODUCTION: There are currently more than 2,000 types of bandages for wounds and burns available in the international market, which causes many doubts as to the best therapeutic indication.
OBJECTIVE: To analyze the effects of induced healing by porous cellulose membrane after complete removal of a skin segment from the back of rats.
METHODS: The study was approved by the research ethics committee of Sociedade Evangélica Beneficente de Curitiba. Forty Wistar rats (Rattus norvegicus) were assessed, randomly distributed as follows: twenty animals in the control group (CG) and 20 animals in the membrane group (MG). In the standardized anatomical region, a total skin segment was resected, using a silicon mold measuring 4 cm in diameter and a scalpel blade, exposing the dorsal muscle fascia. After the surgery, a bandage with dry gauze (CG) or Membracel®, dehydrated and sterile (MG) was applied. The animals were sacrificed 7, 14, 21 and 28 days after the surgery. To do so, five animals in the MG and five in the CG were killed by ether overdose. At macroscopy, the bottom and area of the wound were assessed. To assess the bottom of the wound, the classification by the color system was used as follows: red, yellow and black. Digital planimetry was used to measure the wound area. In the microscopic study, epithelization was qualified after hematoxylin-eosin staining and collagenation after picrosirius staining. P < 0.05 was considered significant.
RESULTS: Comparative analysis between MG and CG animals showed that in the MG group the bottom of the wound presented predominance of red color 14 days after the surgery (n = 10 MG = 40% and CG = 0%; p = 0.0476) and wound open area with significant reduction 21 days after the surgery (MG = 12.71%±4.69 and CG = 24.67%±7.22; p = 0.0317). Complete reepithelization (n = 10 MG = 50% and CG = 10%; p = 0.0476) and mature collagen deposition (n = 10 MG = 50% and CG = 10%; p = 0.0476) were observed 14 days after the surgery for both variables.
CONCLUSION: In this experiment, the use of porous cellulose membrane favored healing of a skin wound in the dorsal region of rats.

Keywords: Porous cellulose membrane, wound color, lesion area, reepithelization, collagen, rats.



Conflict of interest: Nelson Yaguishita was granted a Master's scholarship by CAPES when this study was being carried out. He is not a shareholder nor received support from the manufacturing company.

Manuscript received April 22, 2007, accepted May 10, 2007.



This study was performed at Instituto de Pesquisas Médicas (IPEM) of the Graduate Program in Principles of Surgery at Faculdade Evangélica do Paraná (FEPAR) and at Hospital Universitário Evangélico de Curitiba (HUEC). This master's thesis was approved as partial requisite to obtain the title of MSc.
Advisor: Prof. Dr. Jurandir Marcondes Ribas Filho
Co-advisor: Prof. Dr. Célia Toshie Yamamoto
Presentation: 09/08/2006
Examiners: Prof. Dr. Henrique Jorge Stahlke Júnior (UFPR), Prof. Dr. José Fernando Macedo (FEPAR), Prof. Dr. Hamilton Moreira (FEPAR and UFPR).

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