versão impressa ISSN 1678-7757
J. Appl. Oral Sci. v.15 n.3 Bauru maio/jun. 2007
Carolina Simonetti LodiI; Irene RamiresII; Juliano Pelim PessanIII; Lucimara Teixeira das NevesIV; Marília Afonso Rabelo BuzalafV
IDDS, MSc Student, Araçatuba Dental School, São Paulo State University, Araçatuba, SP, Brazil
IIDDS, MSc in Public Health in Dentistry, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil
IIIDDS, MSc, PhD Student, Araçatuba Dental School, São Paulo State University, Araçatuba, SP, Brazil
VIDDS, MSc, Professor, Hospital for Rehabilitation of Craniofacial Anomalies, University of São Paulo, Bauru, SP, Brazil
VDDS, MSc, PhD, Associate Professor, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil
The increasing consumption of juices, soft drinks and teas among children has increased significantly fluoride ingestion at the age range of risk for development of dental fluorosis. Objective: The purpose of this study was to evaluate fluoride concentrations in some brands of industrialized beverages consumed by children in the city of Bauru, SP, Brazil. Material and Methods: 98 brands of beverages were analyzed, divided into 3 lots, comprising 36, 32 and 30 brands, respectively, for the first, second and third lots. Fluoride concentrations were determined by HMDS-facilitated diffusion, using a fluoride ion-specific electrode (Orion 9409). Results: Fluoride concentrations ranged between 0.04 and 1.76 µg F/mL. It was observed a wide variation in fluoride concentrations among the different brands, as well as the different lots of the same brand. There was no information on fluoride concentrations on the labels of any product. Conclusions: Some of the products analyzed could contribute significantly to the total fluoride intake and, thus, be important risk factors for development of dental fluorosis, which indicates the need of controlling the production of these beverages with respect to fluoride concentration.
Uniterms: Fluoride; Dental fluorosis; Beverages; Children.
Fluoride has been shown to be an effective agent for the control of dental caries over more than five decades. Since 1970, this ion has become increasingly available for populations with and without fluoridated drinking water, in a wide range of vehicles such as dentifrices, mouthrinses, supplements, infant formulas and industrialized beverages, besides to dental products for exclusive professional application2,3,5,7,9,13,15,16,18. In Brazil, fluoridation of dentifrices started in 1980 and these products became an important source of fluoride exposure and ingestion15,19.
The exposure to fluoride from the multiple available sources resulted in a significant improvement in the oral health status of population. Simultaneously, however, this led to an increase in the prevalence of dental fluorosis, mainly in its mild and very mild forms, even in regions not supplied with fluoridated water2,13,16,18. Water has been considered responsible for this increase in 40% of cases. The remaining 60% is attributed to other fluoride sources14. Dental fluorosis results from the excessive ingestion of fluoride during the period of enamel formation and its severity is directly related to the absorbed dose of this ion, in addition to the influence of environmental factors. The clinical appearance of mild dental fluorosis is characterized by bilateral, diffuse opaque, white striations that run horizontally across the enamel. In the more severe forms, the enamel may become discolored and/or pitted2,22.
Significant changes have occurred with respect to children's feeding habits in the last decades, with increasing consumption of industrially processed foods and beverages4,7-9,11,15. A study published in 1997 reported that 56% of the United States population was directly exposed to fluoridated water, but this estimate can be even greater if indirect exposure from foods and beverages produced using fluoridated water is also considered11. Therefore, the impact of fluoridated water is higher when it is used for the processing of foods and beverages2,4,8,9,11.
There are several reports addressing the assessment of fluoride concentrations in foods and beverages consumed by children at the age risk for development of dental fluorosis4-10,12,20. Most of these studies have shown that the fluoride concentration in some products could increase the prevalence of dental fluorosis, if consumed on a regular basis. However, there is no study evaluating fluoride levels in beverages especially produced for children consumption in Brazil, i.e., with packages having sizes and labels directed to these consumers. In addition, most beverages typically consumed by children in Brazil do not present information on fluoride concentration on their labels. Therefore, the purpose of this study was to evaluate fluoride concentrations in some brands of industrialized beverages specifically produced for children consumption.
MATERIAL AND METHODS
Beverages were purchased in supermarkets at the city of Bauru, São Paulo State, Brazil. Products with packages and labels specifically designed for children were chosen in an attempt to represent the most consumed beverages by this population. Products included fruit and soy-based juices, black teas, soft drinks and coconut water. In addition, the beverages were purchased in three different dates, in order to address possible variations among lots of the same product. For lots 1, 2 and 3, respectively, a total of 36, 32 and 30 products were acquired, totalizing 98 beverages. All brands and products were coded.
The products were opened on the day of the analysis. Fluoride concentrations were determined after overnight hexamethyldisiloxane (HMDS)-facilitated diffusion21 as modified by Whitford23 (1996), using a fluoride ion-specific electrode and a miniature calomel reference electrode (Accumet, no. 13-620-79: Fischer Scientific, Pittsburgh, PN, USA), coupled to a potentiometer (model EA 940; Orion Research Inc., Cambridge, MA, USA). During the diffusion process, which was conducted at room temperature, the solutions in the nonwettable Petri dishes (Falcon, No. 1007; Becton Dickinson, Franklin Lakes, NJ, USA) were gently swirled on a rotary shaker. Fluoride standards (10, 50, 100 and 250 nM F) were prepared by serial dilution of a stock-standard containing 0.1 M of fluoride (Orion 940906) in triplicate and diffused in the same manner as the samples. Comparison with identical nondiffused fluoride standards showed that recovery after diffusion was > 99%. The standard curve had a correlation coefficient ³ 0.99. All samples were analyzed in duplicate. The mean repeatability of the fluoride readings, based on the duplicate samples, was 94.9%.
Fluoride concentrations in the different brands and flavors of beverages analyzed in this study are given on Table 1. Fluoride concentrations in the first lot ranged between 0.051 µg F/mL (sample 18) and 0.868 µg F/mL (sample 30). In the second and third lots, respectively, fluoride concentrations ranged from 0.047 µg F/mL (sample 18) to 1.451 µg F/mL (sample 30), and from 0.040 µg F/mL (sample 34) to 0.762 µg F/mL (sample 31).
The juices (including soy-based products) and coconut water presented relatively low fluoride concentrations, varying between 0.040 µg F/mL (sample 34, lot 3) and 0.300 µg F/mL (sample 21, lot 2). With respect to black tea samples, higher fluoride concentrations were detected, ranging from 0.462 µg F/mL (sample 27, lot 1) to 1.762 µg F/mL (sample 31, lot 3).
Foods, beverages, drinking water and dental products are considered as the main sources of fluoride intake for children above 1 year of age2,4,7-9,15,16,18. Most foods have fluoride concentrations lower than 0.5 µg F/g, except for seafood and chicken products, which may present higher fluoride levels due to the inclusion of bone, skin and shells during processing13.
Fluoride concentrations in beverages result from the concentration of this ion in the water used during industrial processing. These concentrations usually range between 0.1 and 1.4 µg F/mL, except for teas, which may have up to 7 µg F/mL4,7-10,12,17,20,24. Most beverages analyzed in the present study showed fluoride concentrations below 0.1 µg F/g. For teas, fluoride levels varied between 0.6 and 1.7 µg F/mL. Considering that the recommended dose of fluoride intake varies between 0.05 and 0.07 mg F/ Kg body weight, the teas analyzed in this study can contribute significantly for the total fluoride intake, which therefore increases the risk for development of dental fluorosis. In this sense, the consumption of 200 mL of a tea containing 1.76 µg F/mL accounts for about 0.03 mg F/ Kg body weight for a child weighing 12 Kg (approximately 2 years old). It is worth emphasizing that this dose (0.03 mg F/ kg) would be reached with the ingestion of only this beverage, not including other foods and beverages consumed during the day. Several reports have demonstrated high fluoride concentrations in foods typically consumed by children, such as powdered milk, ready-to-drink chocolate milks, cereals and snacks1,4,6,8,9. Moreover, the early use of fluoridated dentifrices by children is of concern, mainly due to the inverse relationship between fluoride ingestion from this source and the child's age. Fluoride dentifrices can account up to 80% of total daily fluoride intake by 2-3 year-old children1.
The variation in fluoride concentrations among the lots is another issue that deserves attention. In the present study, significant variations were observed for most analyzed products and differences of 200-300% in lots of the same products were detected (Table 1 beverages 16, 17, 29, 31-34 and 36). For beverages with low fluoride concentrations, these variations may have little impact on total fluoride intake, as for beverages 16, 17, 34 and 36. For products with high fluoride levels, however, such variations can contribute significantly for the total intake of this ion (beverages 29-32). These findings suggest the need of a more effective control in the production and labeling of the evaluated products by the surveillance authorities, as none of them presented this information on their labels.
Although the increase in the prevalence of dental fluorosis verified in the last decades has occurred mainly in the mildest forms, thus not affecting dental function and, many times, not compromising esthetics, this indicates the need for controlling fluoride intake, especially in children below 6 years of age. This control must be based on the correct indication of fluoride use by dental professionals and instructions given to general population concerning the main sources of fluoride intake, in order to make possible the rational use of this non-invasive caries control approach. Furthermore, sanitary surveillance actions must be more effectively reinforced, by means of a systematic analysis, in order to regulate and establish as mandatory the presentation of fluoride concentration on the labels of foods and beverages.
The results of the present study suggest the need of controlling the production of the foods and beverages with respect to fluoride concentration. Specific standardization of the product labels is also needed, by obligating manufacturers to present clear information about fluoride concentration in the products because the widespread consumption of certain beverages along with other sources of fluoride intake could lead to an increase in the prevalence of dental fluorosis.
1- Almeida BS, Cardoso VES, Buzalaf MAR. Fluoride ingestion from toothpaste and diet in 1-3-year-old Brazilian children. Community Dent Oral Epidemiol. 2007;35:53-63. [ Links ]
2- Burt BA. The changing patterns of systemic fluoride intake. J Dent Res. 1992;71(5):1228-37. [ Links ]
3- Buzalaf MAR, Cury JA, Whitford GM. Fluoride exposures and dental fluorosis: a literature review. Rev Fac Odontol Bauru. 2001;9:1-10. [ Links ]
4- Buzalaf MAR, Damante CA, Trevizani LMM, Granjeiro JM. Risk of fluorosis associated to consumption of infant formulas prepared with bottled water. ASDC J Dent Child. 2004;71:110-3. [ Links ]
5- Buzalaf MAR, Almeida BS, Cardoso VES, Olympio KPK, Furlani TA. Total and acid-soluble fluoride content of infant cereals, beverages and biscuits from Brazil. Food Addit Contam. 2004;21:210-5. [ Links ]
6- Buzalaf MAR, Granjeiro JM, Cardoso VES, Silva TL, Olympio KPK. Fluorine content of several brands of chocolate bars and chocolate cookies found in Brazil. Pesqui Odontol Bras. 2003;17:223-7. [ Links ]
7- Buzalaf MAR, Granjeiro JM, Damante CA, Ornelas F. Fluctuations in public water fluoride level in Bauru, Brazil. J Public Health Dent. 2002;62:173-6. [ Links ]
8- Buzalaf MAR, Granjeiro JM, Damante CA, Ornelas F. Fluoride content of infant formulas prepared with deionized, bottled mineral and fluoridated drinking water. ASDC J Dent Child. 2001;68:37-41. [ Links ]
9- Buzalaf MAR, Granjeiro JM, Duarte JL, Taga MLL. Fluoride content of infant foods in Brazil and risk of dental fluorosis. ASDC J Dent Child. 2002;69:196-200. [ Links ]
10- Heilman JR, Kiritsy MC, Levy SM, Wefel JS. Assessing fluoride levels of carbonated soft drinks. J Am Dent Assoc. 1999;130(11):1593-9. [ Links ]
11- Heller KE, Ekuland SA, Burt BA. Dental caries and dental fluorosis at varying water fluoride concentrations. J Public Health Dent. 1997;57:136-43. [ Links ]
12- Jackson RD, Brizendine EJ, Kelly AS, Hinesley R, Stookey GK, Dunipace AJ. The Fluoride content of foods and beverages from negligibly and optimally fluoridated communities. Community Dent Oral Epidemiol. 2002;30:382-91. [ Links ]
13- Levy SM, Kritsy MC, Warren JJ. Sources of fluoride intake in children. J Public Health Dent. 1995;55:39-52. [ Links ]
14- Lewis DW, Banting DW. Water fluoridation: current effectiveness and dental fluorosis. Community Dent Oral Epidemiol. 1994;22:153-8. [ Links ]
15- Lima YBO, Cury JA. Ingestão de flúor por crianças através da água e dentifrício. Rev Saude Publica. 2001;35:576-81. [ Links ]
16- Mascarenhas AK. Risk factors for dental fluorosis: a review of the recent literature. Pediatr Dent. 2000;22:269-77. [ Links ]
17- Pang DTY, Philipps CL, Bawden JW. Fluoride intake from beverage consumption in a sample of North Carolina children. J Dent Res. 1992;71:1382-8. [ Links ]
18- Pendrys DG, Katz RV, Morse DE. Risk factors for enamel fluorosis in a fluoridated population. Am J Epidemiol. 1997;143:808-15. [ Links ]
19- Rodrigues MHC, Bastos JRM, Buzalaf MAR. Fingernails and toenails as biomarkers of subchronic exposure to ffrom dentifrice in 2-3-year-old children. Caries Res. 2004;38:109-14. [ Links ]
20- Silva M, Reynolds EC. Fluoride content of infant formula in Australia. Aust Dent J. 1996;41:37-42. [ Links ]
21- Taves DR. Separation of fluoride by rapid diffusion using hexamethyldisiloxane. Talanta. 1968;15:969-74. [ Links ]
22- Thylstrup A, Fejerskov O. Clinical appearance of dental fluorosis in permanent teeth in relation to histological changes. Community Dent Oral Epidemiol. 1978;6:329-37. [ Links ]
23- Whitford GM. The metabolism and toxicity of fluoride. Basel: Karger; 1996. [ Links ]
24- van Winkle S, Levy SM, Kiritsy MC, Heilman JR, Wefel JS, Marshall T. Water and formula fluoride concentrations: significance for infants fed formula. Pediatr Dent. 1995;17:305-10. [ Links ]
Marília Afonso Rabelo Buzalaf
Alameda Octávio Pinheiro Brisolla, 9-75
17012-901 Bauru, SP, Brasil
Phone: 55 14 32358246 - Fax: 55 14 32262076
Received: November 30, 2006
Modification: February 13, 2007
Accepted: May 07, 2007