In vitro evaluation of the precision of working casts for implant-supported restoration with multiple abutments

Abstract

OBJECTIVE: The purpose of this study was to compare the accuracy of two working cast fabrication techniques using strain-gauge analysis. METHODS: Two working cast fabrication methods were evaluated. Based on a master model, 20 working casts were fabricated by means of an indirect impression technique using polyether after splinting the square transfer copings with acrylic resin. Specimens were assigned to 2 groups (n=10): Group A (GA): type IV dental stone was poured around the abutment analogs in the conventional way; Group B (GB), the dental stone was poured in two stages. Spacers were used over the abutment analogs (rubber tubes) and type IV dental stone was poured around the abutment analogs in the conventional way. After the stone had hardened completely, the spacers were removed and more stone was inserted in the spaces created. Six strain-gauges (Excel Ltd.), positioned in a cast bar, which was dimensionally accurate (perfect fit) to the master model, recorded the microstrains generated by each specimen. Data were analyzed statistically by the variance analysis (ANOVA) and Tukey's test (α= 5%). RESULTS: The microstrain values (µepsilon) were (mean±SD): GA: 263.7±109.07µepsilon, and GB: 193.73±78.83µepsilon. CONCLUSION: There was no statistical difference between the two methods studied.

Implant-supported restoration; Molding; Strain gauges

ORIGINAL ARTICLES

In vitro evaluation of the precision of working casts for implant-supported restoration with multiple abutments

Anderson Almeida Castilho^I; Alberto Noriyuki Kojima^II; Sarina Maciel Braga Pereira^III; Diego Klee de Vasconcellos^II; Marcos Koiti Itinoche^II; Renata Faria^II; Marco Antonio Bottino^IV

^IResearch Fellow, Department of Dental Materials and Prosthodontics, Dental School of São José dos Campos, São Paulo State University "Júlio de Mesquita Filho", São José dos Campos, SP, Brazil

^IIDDS, MS, PhD, Department of Dental Materials and Prosthodontics, Dental School of São José dos Campos, São Paulo State University "Júlio de Mesquita Filho", São José dos Campos, SP, Brazil

^IIIMS, Department of Dental Materials and Prosthodontics, Dental School of São José dos Campos, São Paulo State University "Júlio de Mesquita Filho", São José dos Campos, SP, Brazil

^IVDDS, MSc, PhD, Adjunt Professor, Department of Dental Materials and Prosthodontics, Dental School of São José dos Campos, São Paulo State University "Júlio de Mesquita Filho", São José dos Campos, SP, Brazil

^{Corresponding address}

ABSTRACT

OBJECTIVE: The purpose of this study was to compare the accuracy of two working cast fabrication techniques using strain-gauge analysis.

METHODS: Two working cast fabrication methods were evaluated. Based on a master model, 20 working casts were fabricated by means of an indirect impression technique using polyether after splinting the square transfer copings with acrylic resin. Specimens were assigned to 2 groups (n=10): Group A (GA): type IV dental stone was poured around the abutment analogs in the conventional way; Group B (GB), the dental stone was poured in two stages. Spacers were used over the abutment analogs (rubber tubes) and type IV dental stone was poured around the abutment analogs in the conventional way. After the stone had hardened completely, the spacers were removed and more stone was inserted in the spaces created. Six strain-gauges (Excel Ltd.), positioned in a cast bar, which was dimensionally accurate (perfect fit) to the master model, recorded the microstrains generated by each specimen. Data were analyzed statistically by the variance analysis (ANOVA) and Tukey's test (α= 5%).

RESULTS: The microstrain values (µe) were (mean±SD): GA: 263.7±109.07µe, and GB: 193.73±78.83µe.

CONCLUSION: There was no statistical difference between the two methods studied.

Uniterms: Implant-supported restoration; Molding; Strain gauges.

INTRODUCTION

Failure of passive fit in implant-supported prostheses induces deleterious forces on the implants and their associated components, and can lead to denture failure by fatigue and/or loss of osseointegration^17,20. It is recognized that absolute passive fit is unattainable^11,19, as the clinical and laboratory variables inherent to rehabilitating treatment itself are obstacles to this^9,7,13.

Therefore, the relevance of misfit is considered in view of the materials available and techniques used at present. Branemark, et al.⁶ (1985) has recommended the splinting technique as the clinical conduct of choice for transferring the intraoral posts to the working model. According to this author, this conduct increases the stability of the transfers both during their removal from the impression and at the moment of fixing the analogs, in addition to having shown more consistent results with regard to precision of the impression^5,14. Moreover, polyether and addition silicone impression materials should be preferred for transfer procedures because they have the lowest distortion values (around 100 µm)¹⁵.

The method used for preparation of the working model for implant-supported prostheses should be taken into consideration, as it must be accurate and resistant to dimensional changes⁴. Thus, the expansion of the plaster setting may have a negative influence on positioning the analogs and consequently be a negative factor added to the basic requisites for achieving passive fit. The purpose of this study was to evaluate a variable in the technique used for preparation of working models for manufacturing metal infrastructures (replicas of the abutments were isolated with rubber tubes to prevent contact with the plaster inserted, after the intermediates were fixed to the plaster test specimen), by assessing the influence on the passiveness of the denture structure associated with the indirect technique, and the use of polyether for taking impressions under analysis by strain gauges.

MATERIAL AND METHODS

A homogeneous polyurethane-based experimental model was created (Polyurethane for pouring F16; Axson, Cergy, France). Four external hexagon, self-tapping implants measuring 3.75 mm in diameter by 13.00 mm in length (Master Screw; 517713; Conexão Sistema de Próteses, São Paulo, SP, Brazil) were screwed to 4 Micro-Unit type prosthetic connections (132023; Conexão Sistema de Próteses, São Paulo, SP, Brazil) 3-mm high, belted, with a torque of 20 Ncm (manual torquemeter 400000; Conexão Sistema de Próteses, São Paulo, SP, Brazil) in accordance with the manufacturer's recommendations. The post/implant sets were fixed to a preexistent palladium-silver based, circular shaped metal bar (Williams W1; Ivoclar Vivadent, Amherst, NY, USA), of 4 mm in the buccolingual direction and 3mm in the occlusocervical direction, at a torque of 10 Ncm. This bar was made by the lost wax technique, being over cast on 4 cylinders machined from precious metal (144002; Conexão Sistema de Próteses, São Paulo, SP, Brazil), disposed parallel to each other at a distance of 14 mm from center to center. By means of quality control of the marginal adaptation of the machined cylinders with an optic microscope at X230 magnification (Sprint 100; RAM Optical Instrumentation; Irvine, CA, USA), components that presented a misfit greater than 10 µm in relation to the prosthetic pillars were discarded.

Four parallel holes with slightly larger diameter and length than the implants were previously drilled in the experimental model. The bar/abutments/implants set was fixed to the model by means of re-basing the holes with polyurethane, keeping the abutments to the level of the model surface. Thus, it was sought to diminish the stresses that could be introduced to the bar to the maximum, and thereby minimize the influence on the readings of the strain gauges that would monitor the test specimens.

Four square transfers were screwed to the Micro-Unit type prosthetic connections of the master model at a torque of 10 Ncm. To fabricate each test specimen, the transfers were splinted with resin (G.C. Pattern Resin; G.C. Dental Industrial; Tokyo, Japan). Splinting was standardized by following the same standard measurement of the Ag-Pd bar used in the master model, with polyvinylsiloxane duplication material (Stern Tek; Sterngold Restorative Systems, Attleboro, MA, USA), by the powder/liquid brush technique. Twenty ferrules with identical thickness and length were made. After 17 minutes, the ferrules were cut into three parts at equidistant points from the transfers with a 0.5-mm-thick diamond disk and reunited again with resin by the brush technique.

Twenty impressions were made in polyether material (Impregum F, ESPE, Seefeld, Germany) using the indirect technique by means of an individual mold previously made of chemically activated acrylic resin. Under a constant load of 500 g for 10 minutes, the impressions were kept in position until the final polymerization reaction of the material. All moldings were done in a temperature controlled environment at 26°C and relative humidity of around 50%, maintained by air conditioning and air humidification. Perforations and four top openings were made in the body of the mold to allow free access to the square transfer screws, and in each act of molding, the adhesive for polyether (3M/ESPE, St. Paul, MN, USA) was previously used, and 15 minutes were waited. The standard mold presented internal relief of 2 mm in both directions for the resin ferrules. The contention walls in the mold fitted intimately to the standard model walls, in order to allow the same positioning and involvement to the base at all times, thus allowing afterwards, when the test specimens were made, that the same quantity of molding material and plaster could be used.

When the mold was obtained, replicas of the intermediates were screwed to the transfers at a torque of 10 N and before the volume of plaster for making each test specimen was inserted, a 3.5 cm high dyke of 7 wax (Polidental, Indústria e Comércio Ltda., São Paulo, SP, Brazil) was constructed for each mold, so that the test specimen housed 100 g of Durone type IV plaster (Dentsply, Petrópolis, RJ, Brazil). The 10 test specimens were divided into two groups (A and B), which differed to each other in the manner of insertion of the plaster.

In Group A, the normal procedure was followed, so that the volume of plaster was inserted in the 10 test specimens in the conventional manner, in accordance with the manufacturer's instructions (water-powder ratio). In Group B, each replica of the abutments was isolated with rubber tubes in order to prevent contact with the plaster inserted for making the test specimens. After the plaster had hardened completely, all rubber tubes were removed, and only then the amount of plaster necessary for fixing the intermediates to the plaster test specimen was inserted, also in accordance with the water/powder ratio recommended by the manufacturer (Figure 1).

The water/powder ratio used was suggested by the manufacturer as a guide (19 mL water for each 100 g of plaster), and was measured with a pipette graded for the volume of water and a scale graded for weighing the powder. The mixture was worked with a spatula for 15 seconds in a plastic tub to homogenize it, and mixing complemented by working with a spatula under vacuum for another 1 minute. All test specimens were kept at a constant temperature of 26^oC. A period of 2 hours was waited for the test specimen unmolding procedures and for the micro-deformation readings by means of the strain gauges.

Using the device developed with the metal structure fabricated in Ag-Pd, the 20 test specimens (GA n=10; GB n=10) were assessed by means of the strain-gauges. The influence of type IV plaster setting expansion on the fabrication of the working models was assessed by means of the traction and compression induced in the test specimens.

Two strain gauges (PA-06-060CA-120L; Excel Sensores Ltda., São Paulo, SP, Brazil) were bonded to the top surface on each segment of the bar with cyanoacrylate adhesive (Super Bonder^®; Loctite, São Paulo, SP, Brazil), aligned to the fixing screws and 1 mm distant from their platforms, making a total of 6 strain gauges, which were numbered from 1 to 6, from left to right. Data from the 6 sensors were amplified and transferred with a signal amplifier (ADS 2000IP; Lynxx, São Paulo, SP, Brazil) and were then recorded and later analyzed by a special software (AqDados & AqAnalysis; São Paulo, SP, Brazil). The magnitude of deformation in each strain gauge was recorded in micro-deformation (µe), which is equivalent to the change in length of a strain gauge, divided by its original length (1.52 mm) and multiplied by 10^-6. The appliance was balanced and calibrated to ± 000 µe of deformation, without fastening the screws on the analysis device for each reading (Figure 2).

The screw-fastening sequence, after the test specimens were positioned on the analyzing device followed the sequence proposed by JEMT¹⁰, that is, 2,4,1,3. For Groups A and B, 3 readings were made for each specimen after expansion of the final plaster setting. The deformation produced in each strain gauge was measured and recorded as soon as the four screws were tightened.

This experiment followed a factorial scheme of the 2 x 3 type. The experimental variables, the factors under study were relief (absence and presence) and the strain gauges (positions: S-12, S-34 and S-56). The variable response was the micro-deformation value obtained in the strain-gauge test. The experimental unit was the prosthetic structure. The test specimens were randomly assigned to the relief (spacers of rubber tubes - SRT) conditions.

Sixty (60) data were obtained, that is, six experimental conditions by six repetitions, which were submitted to statistical analysis using the following statistical softwares MiniTab, version 14.12, 2004(Minitab, Inc., State College, PA, USA) and GraphPad Prism version 4.0, 2003 (GraphPad Software Inc., San Diego, CA, USA). The descriptive statistics consisted of the calculation of the means and standard deviations. The inferential statistics consisted of analysis of variance of repeated measurements of two factors (relief [SRT] and strain-gauges), in which the variable strain-gauges was considered as a repeated factor. The study of the interaction effect was conducted by means. Multiple comparisons among the means for the 6 experimental conditions were made by the Tukey's multiple-comparison test. Significance level was set at 5%.

RESULTS

The micro-deformation values (µe) obtained are shown in the scatter diagram in column (dot plot) (Figure 3).

To assess the relationship between the relief and the position of the strain-gauge, the data obtained in the present experiment were submitted to ANOVA, after the suppositions of the analysis of variance model were assessed.

The residual values, resulting from adjustment of the model adopted, were examined to assess the suitability of the model for valid statistical inferences and it was determined that the original data provided an adequate adjustment because they adjusted to a normal distribution of probability, and the uniformity of the residues (homoscedasticity) was furthermore verified by means of the graph of residual values in relation to the adjusted values.

Data on Table 1 show that only the effect of interaction was statistically significant. There was not the same relation between the conditions (with and without relief - spacers of rubber tubes) in each type of strain-gauge (Figure 4). It is possible to state that the difference between the conditions without relief versus with relief (spacers of rubber tubes) is more severe for the strain-gauge type S1.2.

DISCUSSION

ADA's No. 25 specification¹ indicates that the measurements of the final setting expansion of the plaster should be taken two hours after it is mixed, but according to Heshmati, et al.⁸ (2002), higher linear expansion values were found at 120 hours rather than at 2 hours, with 22% to 71% of the total setting expansion occurring after 2 hours. Furthermore, total expansion was completed within 96 hours. According to Winkler, et al.²³ (1998), plaster setting time depends on the measuring method and the setting of plaster in terms of clinical relevance is affected in any case before its final setting, altering its microscopic structure. The clinical relevance of these data are reflected in the fact that the quality of fabrication of prosthetic restorations is also related to the quality of the working models obtained. Weakened models that are easily abraded or distorted have an influence on the rehabilitative treatment. Thus, it is desirable to select a brand of plaster that exhibits expansion patterns and well-defined stability, which must be considered when selecting the commercial brand²³.

Spiekermann, et al.²¹ (1995) and Rubo and Souza¹⁸ (2001) described the strain-gauge, photoelasticity and finite elements analysis as the main investigation and biomechanical analysis methods in implant dentistry.

In the present study using strain-gauge testing, the micro-deformation values were obtained 2 hours after the test specimens were fabricated, in accordance with the ADA's No. 25 specification, in which the final setting expansion of the plaster and its possible effect on displacement of the analogs could occur.

In this study, the powder/liquid brush technique was used. According to Takahashi, et al.²² (1999), when adequately performed, this technique determines lower indices of resin polymerization shrinkage. Mojon, et al.¹⁶ (1990) have stated that the dimensional behavior of resin, when separation and re-uniting are done 17 minutes after the setting reaction, allows considerable reduction (80%) in the effects of polymerization shrinkage, within a period of time that would dispense with the second clinical appointment.

The literature is not unanimous as regards which the best molding technique^2,3,9. Thus, choosing the indirect technique with use of square splinted transfers and following the technique consolidated by Branemark⁶ (1985), limited the influence on the study outcomes. This made possible for all analogs to be positioned in the mold under the same torque, prevented the analogs from rotating while they were being screwed to the square transfers, and also allowed controlling the relationship of the transfers with the molding material. It is assumed that this approach diminished movement during the positioning of the analog replicas.

There are no scientifically based studies referring to modifications in the conventional technique for fabrication of working casts in implant dentistry. In the present study, there was no statistically significant difference between the techniques for preparation of working casts with and without relief. The modified technique with relief did not contribute significantly to the reduction in the mean micro-deformation values, compared to the conventional plaster insertion technique. Only the interaction effect was statistically significant and for each strain-gauge. Although the condition without relief did not differ significantly from the condition with relief, in Sensor S1-S2 the difference between the condition with relief and without relief was more marked (rubber spacers).

From the results of ANOVA and Figure 2, it is possible to state that the difference between the conditions without relief versus with relief followed the same pattern of micro-deformation for the strain-gauges type S1-S2 and S3-S4, but differed from the conditions of strain gauges S5-S6.

Data on Table 2 indicates that the causal effect of the differences in the micro-deformation values obtained was the fact that the sensors (S1-S2 and S5-S6) were placed at the extremities of the bar, and underwent greater effects from the tightening sequence of the screws and the plaster setting expansion. Another hypothesis with regard to the condition of the samples with relief, would be the possibility of the pure residual stress having manifested itself more clearly because its condition of relief did not allow the setting expansion process to influence the results of the readings of strain-gauges S5 and S6. Thus, for didactic effect, the metal bar was divided into three segments (A-S1 and S2, B-S3 and S4 and C-S5 and S6). After the torque of screws 2 and 4, the bar was stabilized at two points. After the torque of screw 1, stress was started in segment A, since segments B and C were stabilized by the torque on screws 2 and 4. After the torque on screw 3, the strain-gauges 5 and 6 were those that most underwent deformation, since screws 1 and 2 and 4 had already been tightened, giving the set greater stability, sensitizing the strain-gauges S5 and S6 that obtained higher micro-deformation value records. Further studies are required with regard to the condition of relief in working models for implant-supported dentures, as well as with respect to the tightening sequence of denture retention screws.

CONCLUSION

The search for excellence in passive fit of prosthetic work must be allied to the knowledge provided by applied research in order to guide the choice for the best techniques and materials, with clinical practice information and the sensitivity of each professional's experience. Considering control of the effects of plaster expansion setting that may contribute to displacement of the analogs, the modified technique for fabricating working models was not significantly different or more precise than the technique in which plaster insertion was done in a single stage, which indicates the advantage of ferruling transfers. Further investigation is necessary to quantify the degree of adaptation to prevent biological and/or mechanical complications in implant-supported prostheses.

Received: February 02, 2007

Modification: April 27, 2007

Accepted: May 14, 2007

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Publication Dates

• Publication in this collection
  24 Aug 2007
• Date of issue
  June 2007

History

• Accepted
  14 May 2007
• Received
  02 Feb 2007
• Reviewed
  27 Apr 2007