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Journal of Venomous Animals and Toxins including Tropical Diseases

On-line version ISSN 1678-9199

J. Venom. Anim. Toxins incl. Trop. Dis vol.11 no.4 Botucatu Oct./Dec. 2005 



Modulation of macrophage activity, induced by b-1,3 polyglucose extracted from Saccharomyces cerevisae, in BALB/c mice infected with Toxoplasma gondii



R. A. de Oliveira




THESIS. R. A. de Oliveira submitted this thesis for his Doctorate in Tropical Diseases at Botucatu School of Medicine, São Paulo State University, UNESP, Botucatu, São Paulo, Brazil, 2004.

Advisor: Professor Sílvio Luis de Oliveira


Toxoplasma gondii is an extremely widespread parasite that chronically infects approximately 20% of the world’s population. b-glucan has been described as a potent immunomodulatory substance. The aim of this work was to evaluate the effects of b -glucan on the macrophage activity in mice infected with T. gondii. BALB/c mice were infected with 104 tachyzoites/ml by the intraperitoneal (ip) route. The suspension of b-glucan used in this study was extracted from the cellular walls of Saccharomyces cerevisae. The animals received 100µg of glucan by ip route. Hydrogen peroxide (H2O2) and nitric oxide (NO) production by peritoneal and splenic macrophage cultures were determined by phenol red oxidation and Griess reaction, respectively. The results showed that b-glucan has a potent immunomodulatory effect in animals infected with T. gondii, and could be useful in the treatment of this zoonosis.

Key words: Toxoplasma gondii, BALB/c mice, b-glucan.



Correspondence to
Sílvio Luís de Oliveira
Departamento de Microbiologia e Imunologia, Instituto de Biociências, UNESP, Campus de Botucatu
18618-000, Botucatu, SP, Brasil

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