SciELO - Scientific Electronic Library Online

 
vol.15 issue1The inhibitory effect of MK886 on the inflammatory process caused by Paracoccidioidomycosis brasiliensis infection in genetically selected miceEffects of selenium supplementation on cattle anti-rabies humoral immune response and levels of serum selenium and cortisol author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Journal of Venomous Animals and Toxins including Tropical Diseases

On-line version ISSN 1678-9199

J. Venom. Anim. Toxins incl. Trop. Dis vol.15 no.1 Botucatu  2009

http://dx.doi.org/10.1590/S1678-91992009000100020 

THESIS

 

Cytokine production in experimental paracoccidioidomycosis of murine selected lineages for acute inflammatory response (air)

 

 

Correspondence to

 

 

Thesis: J. S. Cavalheiro submitted this dissertation for her Doctorate in Tropical Diseases at the Botucatu Medical School, São Paulo State University, UNESP, Botucatu, São Paulo State, Brazil, 2009.

Advisor: Professor Silvio Luis de Oliveira

ABSTRACT

Paracoccidioidomycosis is a systemic human mycosis caused by Paracoccidioides brasiliensis (P. brasiliensis), an imperfect dimorphic fungus whose conidia are its infective form. Mice genetically selected for maximum (AIRmax) and minimum (AIRmin) acute inflammatory response were used as experimental paracoccidioidomycosis models. The animals were intraperitoneally inoculated with P. brasiliensis (strain 18) and killed 6, 12 and 24 hours or 3, 7 and 14 days after infection. In these periods, fragments from their spleen, liver and lung were removed for evaluation of the infection level by fungal cells, assessment of macrophagic activity by peritoneal and splenic macrophages - through the determination of nitric oxide (NO) concentrations and production of pro- and anti-inflammatory cytokines of lung and spleen homogenate supernatants. In the present study, it was observed that AIRmax lineages presented greater control of the infectious process than the AIRmin ones. Regarding NO production, AIRmax animals produced more metabolites in late periods, what may help control the infectious process. Concerning cytokine production, it was observed that the production of INF-gamma, TNF-alpha, IL-1, IL-6, IL-8 and IL-12 were increased in AIRmax lineages in most analyzed organs and periods, thus contributing to the greater resistance exhibited by such lineages against infection, except for IL-4 and IL-10 that showed decreased production in AIRmax lineage, reproducing its suppressive biological effect. From these results, it was observed that the AIRmax lineage was more effective in controlling the infectious process, with an important involvement of the analyzed cytokines. These findings are probably related to the genetically selected factors involved in the acute inflammatory response.

Key words: immune response, Paracoccidioides brasiliensis, cytokines, acute inflammatory response.

 

 

Correspondence to:
Juliana Semim Cavalheiro
Departamento de Microbiologia e Imunologia
Instituto de Biociências, UNESP
Botucatu, SP, Brasil
Phone: +55 14 3811-6058
Email: jscvet@bol.com.br/ jusemim@gmail.com.

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License