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Journal of Venomous Animals and Toxins including Tropical Diseases

versão On-line ISSN 1678-9199

J. Venom. Anim. Toxins incl. Trop. Dis vol.15 no.4 Botucatu  2009

http://dx.doi.org/10.1590/S1678-91992009000400013 

Leptospira spp. antibodies in captive coatis (Nasua nasua Storr, 1780) (Carnivora: Procyonidae)

 

 

Langoni HI; Kawaguchi MFI; Oshika JCII; Da Silva RCI; Teixeira CRII

IZoonosis Research Center, NUPEZO, Veterinary Medicine and Animal Husbandry School, São Paulo State University, UNESP, Botucatu, São Paulo State, Brazil
IIDepartment of Veterinary Surgery and Anesthesiology, Veterinary Medicine and Animal Husbandry School, São Paulo State University, UNESP, Botucatu, São Paulo State, Brazil

Correspondence to

 

 


ABSTRACT

Leptospirosis is a worldwide infection that affects many species, including wild animals. The present study aimed to detect Leptospira spp. antibodies in 17 captive coatis (Nasua nasua) by the microscopic agglutination test (MAT). Nine (52.94%) animals tested positive and the following serovars were identified: Copenhageni (22.22%), Shermani (22.22%), Andamana (11.11%), Wolfii (11.11%) and Pyrogenes (11.11%). Two samples presented coagglutination, one (11.11%) for Hebdomadis and Wolfii, and another (11.11%) for Hebdomadis, Hardjo and Wolfii. The current study revealed the presence of the infection even in animals without any clinical signs, reinforcing the possibility that wild animals in captivity may be infected by leptospire serovars, thus enabling reservoirs.

Keywords: Nasua nasua, Leptospira spp., captive coatis, antibodies, microscopic agglutination test.


 

 

INTRODUCTION

In South America, the family Procyonidae, order Carnivora, is represented by 18 species distributed among six genera, including Nasua spp. In the rest of the world, coatis thrive in different environments, such as deciduous and tropical forests, deserts and chaparral regions. Although these animals are classified as carnivores, they may also feed on fruits (1).

Leptospirosis, a zoonosis present throughout the globe, is naturally transmitted between humans and other animals (2). The etiologic agent responsible for the disease is a spirochete, Sphirochaetales order, Leptospiraceae family, that presents three genera: Leptospira, Leptonema and Turneria. Currently, leptospires are classified into more than 250 serovars. This disease assumes an important epidemiological role because it affects domestic and wild species. Some of them develop subclinical infection and maintain the agent in their kidneys with intermittent elimination through urine, constituting a serious public health problem (3).

Serological studies based on leptospirosis had been performed in many wild species, including coatis (Nasua nasua) (4). In some places, such as zoos and parks, several species must live in restricted areas. This condition can disseminate numerous infectious agents that may cause a wide variety of zoonotic diseases (5).

In order to ascertain important epidemiological aspects of the disease in wild animals, this study aimed to verify the occurrence of Leptospira spp. antibodies in captive coatis and to establish sanitary measures for its control.

Serum samples were collected from 17 captive coatis (Nasua nasua Storr, 1780) in three cities of São Paulo state, Brazil, namely Sorocaba (23º30'06''S, 47º27'29''W), Botucatu (22º53'09''S, 48º26'42''W) and São José dos Campos (23º10'46''S, 45º53'13''W). The study was approved by the Ethics Committee on Animal Experimentation of the Veterinary Medicine and Animal Husbandry School, UNESP, Botucatu, SP, Brazil. They were randomly selected, regardless of age or gender, since they were all healthy. Animals were anesthetized in their enclosures by means of an intramuscular injection with tiletamine-zolazepam (7 mg/kg Zoletil®, Virbac, Brazil) and xylazine (0.5 mg/kg Sedazine®, Fort Dodge, Brazil) employed in pre-anesthesia and induction of anesthesia, respectively.

Subsequently, 3-mL samples of intracardiac blood were collected from each animal and placed in a test tube without anticoagulant. Samples were centrifuged at 1,200 X g for ten minutes and then stored at –80ºC. Afterward, they were submitted to the microscopic agglutination test (MAT), using the following serovars as antigens: Australis, Bratislava, Autumnalis, Butembo, Castellonis, Bataviae, Canicola, Whitcombi, Cynopteri, Djasiman, Sentot, Gryppotyphosa, Hebdomadis, Copenhageni, Icterohaemorraghiae, Javanica, Panama, Pomona, Pyrogenes, Hardjo, Hardjo-prajitno, Hardjo-miniswajezak, Hardjo-C.T.G., Hardjo-bovis, Wolffi, Shermani, Tarassovi, Andamana and Patoc (6, 7). Agglutination of more than 50% leptospires per field was considered to be positive reaction.

Antibodies were detected in nine of 17 (52.94%) serum samples; the results, presented in Table 1, show their identification and the specific serovars with the respective final titer. Serovar titers varied from 100 to more than 3,200.

 

 

Table 2 presents the relation among the number of samples from each zoo, seropositive samples and the prevalence of each serovar. There were eight out of 17 (61.54%) positive results from Sorocaba that reacted against six different serovars. Only one sample from São José dos Campos tested positive. From Botucatu, none of the three samples reacted.

 

 

Wolfii serovar was most frequently detected and with higher titers than the other serovars, ranging from 1,600 to more than 3,200. This serovar most commonly affects humans and other animals in urban areas of Brazil (8).

Although some studies on leptospirosis in feral animals have been carried out in the Americas, Brazil has produced few works on this topic. Therefore, more epidemiological leptospirosis analyses should be performed to develop strategies to control the disease in regions with high density of animals, forest and rivers.

There is little information about the prevalence of leptospirosis in captured animals (9, 10). In captivity, animals of different ecological origins and epidemiological backgrounds are forced to live in close proximity. This may create an opportunity for the dissemination of infectious agents. Hence, zoological gardens and parks can potentially be sources of a wide range of zoonotic diseases (5).

Lins and Lopes (11) reported a leptospiral isolate from a coati (N. nasua) whereas Roth (12) found many serovars present in a racoon (Procyon lotor) in the USA, reinforcing the importance of these species in the leptospirosis transmission chain.

A study involving seven coatis from a Rio de Janeiro zoo, Rio de Janeiro state, was performed using MAT for antibodies against Leptospira spp. Three of them reacted with Icterohemorrhagiae serovar, which characterizes the high suscebility of captive animals, compared with free-living wild animals, to infection by any serovar of Leptospira spp. This corroborates the findings of Girio et al. (4), who did not find any reactive animal among nine Brazilian free-living coatis (13). However, these animals could be infected without any humoral immune response, rendering them reservoirs of the bacterium.

Leptospira spp. infection may occur in Brazilian captive coatis without clinical signs, but with measurable antibodies. Most of the studied animals can be reservoirs of this bacterium and thus require serological tests for leptopsirosis and other zoonoses to ascertain the risks to the environment, park visitors and workers, and its impact on the public health.

 

ACKNOWLEDGEMENTS

We thank The State of São Paulo Research Foundation, FAPESP, for the scientific grant, process n. 2005/02621-6; the Ethics Committee on Animal Experimentation of the Veterinary Medicine and Animal Husbandry School, UNESP; and the animal institutions for the coatis used in the study.

 

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11. Lins ZC, Lopes ML. Isolation of Leptospira from wild forest animals in Amazonian Brazil. Trans R Soc Trop Med Hyg. 1984;78(1):124-6.         [ Links ]

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13. Lilenbaum W, Monteiro RV, Ristow P, Fraguas S, Cardoso VS, Fedullo LPL. Leptospirosis antibodies in mammals from Rio de Janeiro Zoo, Brazil. Res Vet Sci. 2002;73(3):319-21.         [ Links ]

 

 

Correspondence to:
Heliio Langoni
Departamento de Higiene Veterinária e Saúde Pública
Faculdade de Medicina Veterinária e Zootecnia, UNESP
Distrito de Rubião Jr, s/n, Botucatu, SP, 18618-000, Brasil.
Phone: + 55 14 38116270.
Fax: + 55 14 38116075.
Email: hlangoni@fmvz.unesp.br.

Received: February 23, 2009
Accepted: July 6, 2009
Abstract published online: July 8, 2009
Full paper published online: November 30, 2009
Conflicts of interest: There is no conflict.
Financial source: FAPESP, grant 2005/02621-6.

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