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Journal of Venomous Animals and Toxins including Tropical Diseases

On-line version ISSN 1678-9199

J. Venom. Anim. Toxins incl. Trop. Dis vol.16 no.1 Botucatu  2010  Epub Feb 05, 2010

http://dx.doi.org/10.1590/S1678-91992010005000003 

Fine structural analysis of the stinger in venom apparatus of the scorpion Euscorpius mingrelicus (Scorpiones: Euscorpiidae)

 

 

Yigit NI; Benli MII

IDepartment of Biology, Faculty of Science and Arts, Kirikkale University, Kirikkale, Turkey
IIDepartment of Biology, Faculty of Science, Ankara University, Ankara, Turkey

Correspondence to

 

 


ABSTRACT

In this study, the morphology, histology and fine structure of the stinger, a part of the venom apparatus of Euscorpius mingrelicus (Kessler, 1874) (Scorpiones: Euscorpiidae) were studied by light microscopy and transmission electron microscopy (TEM). The stinger, located at the end section of the telson, is sickle-shaped. The venom is ejected through a pair of venom pores on its subterminal portion. Both venom ducts extend along the stinger without contact with each other since they are separated by connective tissue cells. The stinger cuticle is composed of two layers. Additionally, there are many pore canals and some hemolymph vessels in the cuticle. This work constitutes the first histological and fine structure study on Euscorpius mingrelicus stinger.

Key words: scorpion, Euscorpius mingrelicus, telson, stinger, fine structure, transmission electron microscopy.


 

 

INTRODUCTION

The body of a scorpion can be divided into two separate parts: the cephalothorax (head and thorax) and the abdomen. The abdomen, in turn, can be divided into 12 distinct segments, with the last five segments forming the metasoma, most often referred to as the tail. The tail usually curves upwards and towards the body, and contains venom glands. When a scorpion captures its prey in its claws, it strikes the prey with its tail. At the end of the metasoma is the telson, which is a bulb-shaped structure containing the venom gland and a sharp, curved stinger, which is also known as the aculeus to deliver the venom. The venom of scorpions is used for both prey capture and defense.

Worldwide, scorpion stings are the most important cause of arachnid envenoming and are responsible for significant morbidity and pediatric mortality in many parts of Central and South America, the Middle East, Asia, and both northern and southern Africa (1-7).

Human deaths from scorpion bites normally occur in the young, elderly or infirm; scorpions are generally unable to deliver enough venom to kill healthy adults. Some people, however, may be allergic to the venom of some species. Although all scorpions are venomous, the most diverse and widespread family, Buthidae, includes the majority of medically significant scorpion species. Despite their fearsome reputation, about 30 species worldwide have venom sufficiently potent to be considered dangerous to human beings (8). The other scorpion species, however, are only venomous enough to affect small vertebrate animals and insects that are their own prey.

In our study, we attempt to describe the histology and fine structure of the stinger of the scorpion species E. mingrelicus. While E. mingrelicus does not possess venom that is potent enough to be considered dangerous for human health, it has a venom mixture that can affect its prey.

E. mingrelicus is a species belonging to Euscorpiidae family which is widespread in central and southern Europe, and also found in Africa (Mediterranean coast), North America (Mexico), Central America (Guatemala), South America (Brazil, Peru, and Venezuela), and Asia (west, central, south and southeast) (9, 10). One species has become established in some parts of southern England. E. mingrelicus (Kessler 1874) is distributed among many parts of Asia (Georgia, Syria, Turkey, Bosnia, Herzegovina, Bulgaria, Croatia, Greece, Italy, Romania, Russia, Slovenia and Yugoslavia); within Turkey, this species is widely distributed throughout the Black Sea, Marmara, Mediterranean and Aegean regions (11, 12).

 

MATERIALS AND METHODS

Scorpions

In the present study, 12 adult E. mingrelicus scorpions were used. They were collected from under stones in the Camlidere-Camkoru forest (33ºE, 40ºN, Ankara, Turkey) in September 2005. The scorpions were identified and then reared in special cages where they were fed insects and grasshoppers at the Biology Department of Kirikkale University. The microscopic specimens of the telson, which is the last portion of the metasoma, were removed and studied under a stereomicroscope (SMZ800®, Nikon Instech Co. Ltd., Japan).

Transmission Electron Microscopy (TEM)

The stingers were fixed in 3% glutaraldehyde buffered with 0.1 M sodium phosphate buffer (pH 7.2) for two hours and then washed four times with buffer, and post fixed in 1% osmium tetroxide (OsO4) for two hours at +4ºC and then washed four times with buffer. They were then dehydrated in a graded ethanol series (40-100% ethanol). The last stages of dehydration were performed with propylene oxide. After dehydration, the samples were embedded in Araldite® CY 212 (Agar Scientific Ltd., UK) and the araldite blocks were made. Thin sections (60-70 nm) were cut from blocks with glass knives on RMC MT-X® ultramicrotome (Boeckeler Instruments, USA) and mounted on 100-mesh grids. Thin sections were stained with uranylacetate, followed by lead citrate, and examined under a Jeol JEM 100 SX® TEM (Jeol Ltd., Japan) at 80 kV (13).

Light Microscopy

The same araldite blocks prepared for TEM were also used to obtain semi-thin sections (0.5-1.5 µm) for light microscopic examination. Semi-thin sections were cut with glass knives and mounted on slides. The sections were stained with 1% toluidine blue and examined under Leica-DM LS2® light microscope (Leica Microsystems, Germany).

 

RESULTS AND DISCUSSION

The venom apparatus of Euscorpius mingrelicus is situated in the telson and is composed of a pair of venom glands and a stinger. Furthermore, the telson has numerous sensory hairs (Figure 1). Each venom gland has its own venom duct, and these ducts, which carry the venom produced in the venom gland, lie along the stinger starting from the base of the stinger. The stinger, located on the end section of the telson, is in the shape of a sickle. The venom is ejected through a pair of venom pores on the subterminal of the stinger (Figure 1).

In the transverse section of the stinger, both venom ducts can be easily seen by light microscope (Figure 2 - a). The internal layer of the lumen of the venom ducts contains an amorphous layer with chitinous materials called intima. The duct is supported by connective tissue cells and surrounded by a thick cuticle. The gap between the two venom ducts is filled by cells similar to those found in the connective tissue. These cells have a round nucleus and several inclusions. The two venom ducts, separated from each other by connective tissue cells, extend along the stinger without contacting each other(Figure 2 - b, c). The cells arranged on the intima have basal membrane folds and copious mitochondria (Figure 3 - a, b).

The stinger cuticle is composed of exocuticle and endocuticle, although the endocuticle of the stinger is thicker than that of the telson. The exocuticle has homogenous structure; the stinger endocuticle is constructed of alternating layers. There are numerous cuticle pore holes that traverse the cuticle layers vertically and terminate at the surface (Figure 4 - a).

Chitin is secreted by epithelial cells then transported to the cuticle surface by chitin channels. Also, there are some hemolymph vessels in the cuticle.

The inner surface of hemolymph vessels is covered with a layer of epithelial cells known as endothelium. These epithelial cells have several microvilli on the parts of the apical surface, which face hemolymph vessel lumen (Figure 4 - b, c).

This region presented defense cells including lymphocytes and macrophages. This cell has undulated cell membrane, pseudopods and groups of microtubules in its cytoplasm (Figure 4 - b, d). Given its structure and morphology, it may be said that this cell is mobile and probably defensive.

Many articles on scorpion venoms, venom chemistry and their medical use are available in the literature (14-19). However, in order to be able to understand the chemistry and physiology of the venom, one should first know the anatomy, histology and fine structure of the venom gland and the venom stinger. The histology and fine structure of the venom gland in several scorpion species have been investigated by several researchers, but the fine structure of venom ducts were not analyzed (20-26).

In our study, the stinger, a part of the venom apparatus of E. mingrelicus (Scorpiones: Euscorpiidae), which is a scorpion species belonging to Euscorpiidae family, was studied in relation to its morphology, histology and fine structure. This study is the first work to examine the fine structure of the stringer and will constitute the foundation for future works.

 

REFERENCES

1. Groshong TD. Scorpion envenomation in eastern Saudi Arabia. Ann Emerg Med. 1993;22(9):1431-7.         [ Links ]

2. Dehesa-Davila M, Possani LD. Scorpionism and serotherapy in Mexico. Toxicon. 1994;32(9):1015-8.         [ Links ]

3. Freire-Maia L, Campos JA, Amaral CF. Approaches to the treatment of scorpion envenoming. Toxicon. 1994;32(9):1009-14.         [ Links ]

4. Ismail M. The treatment of the scorpion envenoming syndrome: the Saudi experience with serotherapy. Toxicon. 1994;32(9):1019-26.         [ Links ]

5. Bergman NJ. Clinical description of Parabuthus transvaalicus scorpionism in Zimbabwe. Toxicon. 1997;35(5):759-71.         [ Links ]

6. Abroug F, Elatrous S, Nouira S, Haguiga H, Touzi N, Bouchoucha S. Serotherapy in scorpion envenomation: a randomised controlled trial. Lancet. 1999;354(9182):906-9.         [ Links ]

7. Ghalim N, El-Hafny B, Sebti F, Heikel J, Lazar N, Moustanir R, Benslimane A. Scorpion envenomation and serotherapy in Morocco. Am J Trop Med Hyg. 2000;62(2):277-83.         [ Links ]

8. Brownell P, Polis G, editors. Scorpion biology and research. Oxford: Oxford University Press; 2001. p. 159-83.         [ Links ]

9. Soleglad ME, Fet V. High-level systematics and phylogeny of the extant scorpions (Scorpiones: Orthosterni). Euscorpius. 2003;11(1):1-175.         [ Links ]

10. Soleglad ME, Sissom WD. Phylogeny of the family Euscorpiidae Laurie, 1896: a major revision. In: Fet V, Selden PA, editors. Scorpions 2001. In Memoriam Gary A. Polis. Buckinghamshire: British Arachnological Society, Burnham Beeches; 2001. p. 25-111.         [ Links ]

11. Fet V, Braunwalder ME. The scorpions (Arachnida: Scorpiones) of the Aegean area: current problems in taxonomy and biogeography. Belg J Zool. 2000;130 (Suppl. 1):17-22.         [ Links ]

12. Fet V, Karataş Ay, Fet EV, Karataş A. First data on the molecular phylogeny of Euscorpius (Scorpiones: Euscorpiidae) from Turkey. Entomol Rev. 2003;83(Suppl. 2):249-52.         [ Links ]

13. Hayat MA. Principles and tecniques of electron microscopy. 2nd ed. London: Edward Arlond; 1981. 522 p. 1 vol.         [ Links ]

14. Zamudio FZ, Gurrola GB, Arevalo C, Srreekumar R, Walker JW, Valdivia HH, et al. Primary structure and synthesis of imperatoxin A (IpTxa) a peptide activator of Ca2+ release channels/ryanodine receptors. FEBS Letters. 1997;405(3):385-9.         [ Links ]

15. Possani LD, Becerril B, Delepierre M, Tytgat J. Scorpion toxins specific for Na+-channels. Eur J Biochem. 1999;264(2):287-300.         [ Links ]

16. Torres-Larios A, Gurrola GB, Zamudio FZ, Possani LD. Hadrurin, a new antimicrobial peptide from the venom of the scorpion Hadrus aztecus. Europ J Biochem. 2000;267(1):5023-31.         [ Links ]

17. Corzo G, Escoubas P, Villages E, Barnham KJ, He W, Norton RS, et al. Characterization of unique amphipathic antimicrobial peptides from venom of the scorpion Pandinus imperator. Biochem J. 2001;359(Pt 1):35-45.         [ Links ]

18. Zeng XC, Peng F, Luo F, Zhu SY, Liu H, Li, WX. Molecular cloning and characterization of four scorpions K+ channel toxins: a new subfamily of venom peptides (alpha-KTx-14) and genomic analysis of a member. Biochimie. 2001;83(1):883-9.         [ Links ]

19. Moerman L, Bosteels S, Noppe W, Willems J, Clynen E, Schoofs L, et al. Antibacterial and antifungal properties of alpha-helical, cationic peptides in the venom of scorpions from southern Africa. Eur J Biochem. 2002;269(19):4799-810.         [ Links ]

20. Pawlowsky EN. Skorpiotomische mitteilungen. I. Ein beitrag zur morphologie der giftdrüsen der skorpione. Z Wiss Zool. 1913;105(1):157-77.         [ Links ]

21. Pawlowsky E. Studies on the organization and development of scorpions. Quart J Micr Sci. 1924;68(1):615-40.         [ Links ]

22. Samano-Bishop A, Ferriz MG. Estudio morfologico, histologico e histoquimico de la glandula venenosa de algunas especies dea alacranes de los generos Vejovis C.L. Koch, Diplocentrus y Centruroides Marx. Ann Inst Biol Mexico. 1964;35(1):139-55.         [ Links ]

23. Mazurkiewicz JE, Bertke EM. Ultrastructure of the venom gland of the scorpion, Centruroides sculpturatus (Ewing). J Morphol. 1972;137(1):365-84.         [ Links ]

24. Kanwar U, Sharma A, Nagpal N. Morphological and cytochemical studies on the venom secreting cells of the scorpion Buthus tamulus. J Anim Morphol Physiol. 1981;28(1):206-9.         [ Links ]

25. Taib NT, Jarrar BM. Histological and histochemical characterization of the venom apparatus of Palestine yellow scorpion, Leiurus quinquestriatus Hemprich & Ehrenberg 1828. Trop Zool. 1993;6(1):143-152.         [ Links ]

26. Quiroga M, Marval MJ, Parrilla-Alvarez P, Sousa L. Tityus caripitensis sp. Scorpion venom gland histology. Toxicon. 1998;36(1):1269.         [ Links ]

 

 

Correspondence to:
Nazife Yigit
University of Kirikkale, Faculty of Science and Arts, Department of Biology
71450, Yahsihan, Kirikkale, Turkey
Phone: +90 318 3574242/1531. Fax: +90 318 3572461
Email: naz_yigit2@hotmail.com

Received: March 24, 2009
Accepted: August 13, 2009
Abstract published online: August 24, 2009
Conflicts of interest: There is no conflict.

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