On the determination of a conversion factor from labelled thymidine incorporation by bacteria to cell production in a sub-tropical estuary: preliminary results

Barrera-Alba, José Juan; Gianesella, Sônia Maria Flores; Saldanha-Corrêa, Flávia Marisa Prado; Moser, Gleyci Aparecida Oliveira

Bell, R. T. 1993. Estimating production of heterotrophic bacterioplankton via incorporation of tritiated thymidine. In: Kemp, P.; Sherr, B.; Sherr, E. & Cole, J. eds. Handbook of methods in aquatic microbial ecology. Lewis Publishers, Inc. p. 495-503.
Bell, T. B.; Ahlgren, G. M. & Ahlgren, I. 1983. Estimating bacterioplankton production by measuring ³H-thymidine incorporation in a eutrophic Swedish lake. Appl. environ. Microbiol., 45:1709-1721.
Bray, G. A. 1960. A simple efficient liquid scintillator for counting aqueous solution in a liquid scintillation counter. Analyt. Biochem., 1:279-285.
Ducklow, H. W. & Hill, S. 1985. Tritiated thymidine incorporation and the growth of heterotrophic bacteria in warm core rings. Limnol. Oceanogr., 30:260-272.
Fuhrman, J. A. & Azam, F. 1980. Bacterioplankton secondary production estimates for coastal waters of British Columbia, Antarctica, and California. Appl. environ. Microbiol., 39:1085-1095.
Hollibaugh, J. T. 1988. Limitations of the [³H]thymidine method for estimating bacterial productivity due to thymidine metabolism. Mar. Ecol. Prog. Ser., 43:19-30.
Kirchman, D. L. & Hoch, M. P. 1988. Bacterial production on Delaware Bay estuary estimated from thymidine and leucine incorporation rates. Mar. Ecol. Prog. Ser., 45:169-178.
Kirchman, D.; Ducklow, H. & Mitchell, R. 1982. Estimates of bacterial growth from changes in uptake rates and biomass. Appl. environ. Microbiol., 49:1296-1307.
Moriarty, D. J. W. 1984. Measurements of bacterial growth rates in some marine systems using the incorporation of tritiated thymidine into DNA. In: Hobbie, J. E. & Williams, P. J. le B eds. Heterotrophics activity in the sea. New York, Plenum Press. p. 217-231.
Moriarty, D. J. W. & Pollard, P. C. 1981. DNA synthesis as a measure of bacterial productivity in seagrass sediments. Mar. Ecol. Prog. Ser., 5:151-156.
Porter, K. G. & Feig, Y. S. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr., 25(5):943-948.
Riemann, B.; Fuhrman, J. A. & Azam, F. 1982. Bacterial secondary production in freshwater measured by ³H-thymidine incorporation method. Microb. Ecol., 8:101-114.
Riemann, B.; Bjørnsen, P. K.; Newell, S. & Fallon, R. 1987. Calculation of cell production of coastal marine bacteria based on measured incorporation of ³H-thymidine. Limnol. Oceanogr., 32:471-476.
Smith, D. C. & Azam, F. 1992. A simple, economical method for measuring bacterial protein synthesis rates in seawater using ³H-leucine. Mar. microbiol. Food Webs, 6(2):107-114.

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Publication in this collection
16 Jan 2009
Date of issue
Dec 2004

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] Bell, R. T. 1993. Estimating production of heterotrophic bacterioplankton via incorporation of tritiated thymidine. In: Kemp, P.; Sherr, B.; Sherr, E. & Cole, J. eds. Handbook of methods in aquatic microbial ecology. Lewis Publishers, Inc. p. 495-503.

[2] Bell, T. B.; Ahlgren, G. M. & Ahlgren, I. 1983. Estimating bacterioplankton production by measuring ³H-thymidine incorporation in a eutrophic Swedish lake. Appl. environ. Microbiol., 45:1709-1721.

[3] Bray, G. A. 1960. A simple efficient liquid scintillator for counting aqueous solution in a liquid scintillation counter. Analyt. Biochem., 1:279-285.

[4] Ducklow, H. W. & Hill, S. 1985. Tritiated thymidine incorporation and the growth of heterotrophic bacteria in warm core rings. Limnol. Oceanogr., 30:260-272.

[5] Fuhrman, J. A. & Azam, F. 1980. Bacterioplankton secondary production estimates for coastal waters of British Columbia, Antarctica, and California. Appl. environ. Microbiol., 39:1085-1095.

[6] Hollibaugh, J. T. 1988. Limitations of the [³H]thymidine method for estimating bacterial productivity due to thymidine metabolism. Mar. Ecol. Prog. Ser., 43:19-30.

[7] Kirchman, D. L. & Hoch, M. P. 1988. Bacterial production on Delaware Bay estuary estimated from thymidine and leucine incorporation rates. Mar. Ecol. Prog. Ser., 45:169-178.

[8] Kirchman, D.; Ducklow, H. & Mitchell, R. 1982. Estimates of bacterial growth from changes in uptake rates and biomass. Appl. environ. Microbiol., 49:1296-1307.

[9] Moriarty, D. J. W. 1984. Measurements of bacterial growth rates in some marine systems using the incorporation of tritiated thymidine into DNA. In: Hobbie, J. E. & Williams, P. J. le B eds. Heterotrophics activity in the sea. New York, Plenum Press. p. 217-231.

[10] Moriarty, D. J. W. & Pollard, P. C. 1981. DNA synthesis as a measure of bacterial productivity in seagrass sediments. Mar. Ecol. Prog. Ser., 5:151-156.

[11] Porter, K. G. & Feig, Y. S. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr., 25(5):943-948.

[12] Riemann, B.; Fuhrman, J. A. & Azam, F. 1982. Bacterial secondary production in freshwater measured by ³H-thymidine incorporation method. Microb. Ecol., 8:101-114.

[13] Riemann, B.; Bjørnsen, P. K.; Newell, S. & Fallon, R. 1987. Calculation of cell production of coastal marine bacteria based on measured incorporation of ³H-thymidine. Limnol. Oceanogr., 32:471-476.

[14] Smith, D. C. & Azam, F. 1992. A simple, economical method for measuring bacterial protein synthesis rates in seawater using ³H-leucine. Mar. microbiol. Food Webs, 6(2):107-114.

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On the determination of a conversion factor from labelled thymidine incorporation by bacteria to cell production in a sub-tropical estuary: preliminary results

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