Evaluation of vital stains for free-living marine nematodes

Thomas, Micheli Cristina; Lana, Paulo da Cunha

AUSTEN, M. C.; SOMERFIELD, P. J. A community level sediment bioassay applied to an estuarine heavy metal gradient. Mar. environ. Res, v. 43, p. 315-328, 1997.
BIRD, A. F.; BIRD, J. The Structure of Nematodes San Diego: Academic Press, 1991. 316 p.
CHU, F. L. E.; VOLETY, A. K.; HALE, R. C.; HUANG, Y. Cellular responses and disease expression in oysters (Crassostea virginica) exposed to suspended field-contaminated sediments. Mar. environ. Res, v. 53, p. 17-35, 2002.
DE NIRO, M. J.; EPSTEIN, S. S. Influence of diet on distribution of carbon isotopes in animals. Geochim. Cosmochim. Acta, v. 42, p.495-506, 1978.
EPSTEIN, S. S.; SHIARIS, M. P. Rates of microbenthic and meiobenthic bacterivory in a temperate muddy tidal flat community. Appl. environ. Microbiol, v. 58:, p. 2426-2431, 1992.
HAMELS, I.; MUYLAERT, K.; CASTELEYN, G.; VYVERMAN, W. Uncoupling of bacterial production and flagellate grazing in aquatic sediments: a case study from an intertidal flat. Aquat. Microbial Ecol., v. 25, p. 31-42, 2001.
HIGGINS, R. P.; THIEL, H. Introduction to the study of Meiofauna Washington, D.C.: Smithsonian Institution Press, 1988. 488 p.
HOWEY, R. L. Vital staining for Protozoa and related temporary mounting techniques. 2000. <http://www.microscopy.uk.org.uk/mag/indexmag.html? http://www.microscopyuk.org.uk/mag/artfeb00/rhvital.html>
LEE, D. L. The physiology of Nematodes Edinburgh;London: Oliver & Boyd, 1965. 154 p. University Reviews in Biology: 3.
LLEWELLY, B.<http://stainsfile.info/StainsFile/index.html>
» link
MAIA, V. 1979. Técnica histológica 2^ª ed. São Paulo: Atheneu , 1979. 246 p.
SANDERSON, J. B. Biological microtechnique Preston: BIOS Scientific Publishers, 1994. 224 p.
SCHRATZBERGER, M.; REES, H. L. BOYD, S. E. Effects of simulated deposition of dredged material on structure of Nematode assemblages - the role of contamination. Mar. Biol, v. 137, p. 613-622, 2000.
SOMERFIELD, P. J;, WARWICK, R. M.; MOENS, T. Meiofauna Techniques. In: ELEFTHERIOU, A.; MCINTYRE, A. (Ed.). Methods for study of marine Benthos 3rd ed. Oxford; Malden,MA: Blackwell Science, 2005. p. 229-245.
STARINK, M.; KRYLOVA, I. N.; BARGILISSEN, M. J.; BAK, R. P. M.; CAPPENBERG, T. E. Rates of benthic protozoan grazing on free and attached sediment bacteria measured with fluorescently stained sediment. Appl. environ. Microbiol,v. 60:, p. 2259-2264, 1994.
UHLIG, G.; THIEL, H. ; GRAY, J. S. The quantitative separation of meiofauna: A comparison of methods. Helgolander Meeresunters, v. 25, p. 173-195, 1973.
ULLBERG, J.; ÓLAFSSON, E. Free-living marine nematodes actively choose habitat when descending from the water column. Mar. Ecol.Prog. Ser, v. 260: 141-149, 2003.
WALTON, M. Techiniques for recognition of living foraminifera. Contr. Cushman Fdn. foram. Res, v. 3, p. 56-60, 1952.

Publication Dates

Publication in this collection
25 Sept 2008
Date of issue
Sept 2008

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] AUSTEN, M. C.; SOMERFIELD, P. J. A community level sediment bioassay applied to an estuarine heavy metal gradient. Mar. environ. Res, v. 43, p. 315-328, 1997.

[2] BIRD, A. F.; BIRD, J. The Structure of Nematodes San Diego: Academic Press, 1991. 316 p.

[3] CHU, F. L. E.; VOLETY, A. K.; HALE, R. C.; HUANG, Y. Cellular responses and disease expression in oysters (Crassostea virginica) exposed to suspended field-contaminated sediments. Mar. environ. Res, v. 53, p. 17-35, 2002.

[4] DE NIRO, M. J.; EPSTEIN, S. S. Influence of diet on distribution of carbon isotopes in animals. Geochim. Cosmochim. Acta, v. 42, p.495-506, 1978.

[5] EPSTEIN, S. S.; SHIARIS, M. P. Rates of microbenthic and meiobenthic bacterivory in a temperate muddy tidal flat community. Appl. environ. Microbiol, v. 58:, p. 2426-2431, 1992.

[6] HAMELS, I.; MUYLAERT, K.; CASTELEYN, G.; VYVERMAN, W. Uncoupling of bacterial production and flagellate grazing in aquatic sediments: a case study from an intertidal flat. Aquat. Microbial Ecol., v. 25, p. 31-42, 2001.

[7] HIGGINS, R. P.; THIEL, H. Introduction to the study of Meiofauna Washington, D.C.: Smithsonian Institution Press, 1988. 488 p.

[8] HOWEY, R. L. Vital staining for Protozoa and related temporary mounting techniques. 2000. <http://www.microscopy.uk.org.uk/mag/indexmag.html? http://www.microscopyuk.org.uk/mag/artfeb00/rhvital.html>

[9] LEE, D. L. The physiology of Nematodes Edinburgh;London: Oliver & Boyd, 1965. 154 p. University Reviews in Biology: 3.

[10] LLEWELLY, B.<http://stainsfile.info/StainsFile/index.html>
» link

[11] MAIA, V. 1979. Técnica histológica 2^ª ed. São Paulo: Atheneu , 1979. 246 p.

[12] SANDERSON, J. B. Biological microtechnique Preston: BIOS Scientific Publishers, 1994. 224 p.

[13] SCHRATZBERGER, M.; REES, H. L. BOYD, S. E. Effects of simulated deposition of dredged material on structure of Nematode assemblages - the role of contamination. Mar. Biol, v. 137, p. 613-622, 2000.

[14] SOMERFIELD, P. J;, WARWICK, R. M.; MOENS, T. Meiofauna Techniques. In: ELEFTHERIOU, A.; MCINTYRE, A. (Ed.). Methods for study of marine Benthos 3rd ed. Oxford; Malden,MA: Blackwell Science, 2005. p. 229-245.

[15] STARINK, M.; KRYLOVA, I. N.; BARGILISSEN, M. J.; BAK, R. P. M.; CAPPENBERG, T. E. Rates of benthic protozoan grazing on free and attached sediment bacteria measured with fluorescently stained sediment. Appl. environ. Microbiol,v. 60:, p. 2259-2264, 1994.

[16] UHLIG, G.; THIEL, H. ; GRAY, J. S. The quantitative separation of meiofauna: A comparison of methods. Helgolander Meeresunters, v. 25, p. 173-195, 1973.

[17] ULLBERG, J.; ÓLAFSSON, E. Free-living marine nematodes actively choose habitat when descending from the water column. Mar. Ecol.Prog. Ser, v. 260: 141-149, 2003.

[18] WALTON, M. Techiniques for recognition of living foraminifera. Contr. Cushman Fdn. foram. Res, v. 3, p. 56-60, 1952.

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Evaluation of vital stains for free-living marine nematodes

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