Quantification and distribution of vibrio species in water from an estuary in Ceará-Brazil impacted by shrimp farming

Costa, Renata A; Silva, Giselle C; Peixoto, Jackson R. O; Vieira, Gustavo H. F; Vieira, Regine H. S. F

Abstracts

Vibrios were quantified and their distribution determined for the estuary of the Coreaú river, in Northeastern Brazil, based on 24 water samples collected between June and October 2005. The most probable number of vibrios per 100 mL ranged from 230 x 10³ to 240 x 10(11). The pH value was the environmental factor most strongly associated with the abundance of vibrios. Sixty-two vibrio strains were isolated belonging to 15 species (6 of which observed in June-September and 8 in October). The most frequently isolated species were V. parahaemolyticus and V. cholerae.

Vibrio; Estuary; Shrimp farming

Foram realizadas análises concernentes à quantificação e distribuição de Vibrio em 24 amostras de água do estuário do Rio Coreaú (CE) no período de junho a novembro de 2005. O Número Mais Provável (NMP/100 mL) de Vibrio oscilou de 230 x 10³ a 240 x 10(11). O pH foi o fator ambiental que mais favoreceu ao aumento da microbiota de Vibrio. Foram isoladas 62 cepas de Vibrio, sendo observada uma distribuição de sete espécies nos meses de junho a setembro, e oito espécies no mês de outubro. As espécies mais freqüentes nos isolamentos foram V. parahaemolyticus e V. cholerae.

Vibrio; Estuário; Carcinicultura

AGUIRRE-GUZMÁN, G.; VÁZQUEZ-JUÁREZ, R.; ASCENCIO, F. Differences in the susceptibility of American white shrimp larval substages (Litopenaeus vannamei) to four vibrio species. J Invertebr Pathol., v. 78, n. 4, p. 215-219, 2001
ALAVANDI, S. V.; MANORANJITA, V.; VIJAYAN, K. K.; KALAIMANI, N.; SANTIAGO, T. C. Phenotypic and molecular typing of Vibrio harveyi isolates and their pathogenicity to tiger shrimp larvae. Letts appl. Microbiol., v. 43, n. 5, p. 566-570, 2006.
ALSINA, M.; BLANCH, A. R. A set of keys for biochemical identification of environment Vibrio species. J. appl. Bact., v. 76, n. 1, p. 79-85, 1994a.
ALSINA, M.; BLANCH, A. R. Improvement and update of a set of keys for biochemical identification of environmental Vibrio species. J. appl. Bact., v. 77, p. 719-721, 1994b.
ALVAREZ, R. J. D.; AGURTO, C; OBREGÓN, J.; PEROZA, L. Detección de Baculovirus penaei y de casos de vibriosis em Litopenaeus vannamei and L. stylirostris en uma granja de la costa occidental de Venezuela. Revta cient. FCV-LUZ, v. 13, n. 4, p. 255-262, 2003.
BARBIERI, E.; FALZANO, L.; FIORENTINI, C.; PIANETTI, A.; BAFFONE, W.; FABBRI, A.; MATARRESE, P.; CASIERE, A.; KATOULI, M.; KÜHN, I.; MÖLLBY, R.; BRUSCOLINI, F.; DONELLI, G. Occurrence, diversity, and pathogenicity of halophilic Vibrio spp. and non-O1 Vibrio cholerae from estuarine waters along the Italian Adriatic Coast. Appl. environ.Microbiol., v. 65, n. 6, p. 2748, 2753, 1999.
BLACKSTONE, G. M.; NORDSTROM, J. L.; VICKERY, M. C.; BOWEN, M. D.; MEYER, R. F.; DEPAOLA, A. Detection of pathogenic Vibrio parahaemolyticus in oyster enrichments by real time PCR. J Microbiol Meth., v. 53, n. 2, p. 149-155, 2003.
BLACKWELL, K. D.; OLIVER, J. D. The ecology of Vibrio vulnificus, Vibrio cholerae, and Vibrio parahaemolyticus in North Carolina estuaries. J. Microbiol., v. 46, n. 2, p. 146-153, 2008.
CERVINO, J. M.; HAYES, R. L.; POLSON, S. W.; POLSON, S. C.; GOREAU, T.J.; MARTINEZ, R. J; SMITH, G. W. Relationship of Vibrio species infection and elevated temperatures to yellow blotch/band disease in Caribbean corals. Appl. environ. Microbiol., v. 70, n. 11, p. 6855-6864, 2004.
CONEJERO, M.J.U.; HEDREYDA, C. T. Isolation of partial toxR gene of Vibrio harveyi and design of toxR-targeted PCR primers for species detection. J. appl. Microbiol., v. 95, n. 3, p. 602-611, 2003.
DAS, B.; KHAN, Y. S.; DAS, P. Environmental impact of aquaculture-sedimentation and nutrient loadings from shrimp culture of the southeast coastal region of the Bay of Bengal. J environ. Sci., (China), v. 16, n. 3, p. 466-470, 2004.
DONOVAN, T. J.; NETTEN, P. Culture media for the isolation and enumeration of pathogenic Vibrio species in food and environmental samples. Int. J. Food Microbiol., v. 26, p. 77-91, 1995.
FUENZALIDA, L.; ARMIJO, L.; ZABALA, B.; HERNÁNDEZ, C.; RIOSECO, M. L.; RIQUELME, C.; ESPEJO, R. T. Vibrio parahaemolyticus strains isolated during investigation of the summer 2006 seafood related diarrhea outbreaks in two regions of Chile. Int. J. Food Microbiol., v. 117, p. 270-275, 2007.
GARTHRIGHT, W. E. Appendix 2: most probable number from serial dilutions. In: FOOD AND DRUG ADMINISTRATION - FDA. Bacteriological analytical manual on line. FDA/CFSAM, 2001. Available: <http://www.cfsan.fda.gov/~edam/bam-a2.html>. Accessed: 06/09/2004.
GONÇALVES, E. G. R.; LOPES, M. J. S.; OLIVEIRA, E. G.; HOFER, E. Avaliação de Vibrio cholerae com o zooplâncton de águas estuárias da Baía de São Marcos/São Luís - MA, Brasil. Rev. Soc. Bras. Med. Trop., v. 37, n. 4, p. 318-323, 2004.
HEIDELBERG, J.; HEIDELBERG, K. B; COLWELL, R. R. Bacteria of the gamma-subclass Proteobacteria associated with zooplankton in Chesapeake Bay. Appl. environ. Microbiol., v. 68, p. 5498-5507, 2002.
HERVIO-HEATH, D.; COLWELL, R. R.; DERRIEN, A.; ROBERT-PILLOT, A. FOURNIER, J. M.; POMMEPUY, M. Occurrence of pathogenic vibrios in coastal areas of France. J. appl. Microbiol., v. 92, p. 1123-1135, 2002.
KAYSNER, C. A.; DEPAOLA JR., A. Vibrio cholerae, V. parahaemolyticus, V. vulnificus, and other Vibrio spp. 2004. Available:<http://www.cfsan.fda.gov/~ebam/bam9.html#authors> Accessed: 10/01/2006.
LACERDA, L. D. Inputs of nitrogen and phosphorus to estuaries of northeastern Brazil from intensive shrimp farming. Braz. J. aquat. Sci. Technol., v. 10, n. 2, p. 13-27, 2006.
LEE, J-K.; JUNG, D-W.; EOM, S-Y.; OH, S-W; KIM, Y.; KWAK, H-S.; KIM, Y-H. Occurrence of Vibrio parahaemolyticus in oysters from Korean retail outlets. Food Control, v. 19, p. 990-994, 2008.
MACÍAN, M. C.; ROSA AZNAR, C. R. A.; GARAY, E. AND PUJALTE, M. J. Identification of Vibrio spp (other than V. vulnificus) recovered on CPC agar from marine natural samples. Int. Microbiol., v. 3, p. 51-53, 2000.
MEIRELES, A. J. A.; VICENTE DA SILVA, E. Abordagem geomorfológica para a realização de estudos integrados para o planejamento e gestão em ambientes flúvio-marinhos. Scripta Nova Revista electrónica de geografía y ciencias sociales, Universidad de Barcelona, v. 6, n. 118, 2002.
MOLITORIS, E.; JOSEPH, S. W.; KRICHEVSKY, M.; SINDHUHARDJA, W.; R. R. COLWELL. Characterization and distribution of Vibrio alginolyticus and Vibrio parahaemolyticus isolated in Indonesia. Appl. environ. Microbiol., v. 50, n. 6, p. 1388-1395, 1995.
MURAKAMI, K.; FUSE, H.; TAKIMURA, O.; KAMIMURA, K.; YAMAOKA, Y. Phylogenetic analysis of marine environmental strains of Vibrio that produce aerobactin. J. mar. Biotechnol., v. 6, n. 2, p. 76-79, 1998.
PÁEZ-OSUNA, F.; GUERRERO-GALVÁN, S. R.; RUIZ-FERNÁNDEZ, A. C. The environmental impact of shrimp aquaculture and the coastal pollution in Mexico. Mar. Pollut. Bull., v. 36, n. 1, p. 65-75, 1998.
PÁEZ-OSUNA, F. The environmental impact of shrimp Aquaculture: Causes, effects, and mitigating alternatives. Environ. Mgmt, v. 28, n. 1, p. 131-140, 2001.
PFEFFER, C. S.; HITE, M. F.; OLIVER, J. D. Ecology of Vibrio vulnificus in estuarine waters of Eastern North Carolina. . Appl. environ. Microbiol., v. 69, n. 6, p. 3526-3531, 2003.
SERRA, C. L. M.; CAVALCANTE, P. R.; ALVES, L. M. C.; NASCIMENTO, A.R.; DINIZ, S.C.C.S. Avaliação de parâmetros físico-químicos e pesquisa de Vibrio parahaemolyticus em águas do estuário do rio Anil (São Luís, Estado do Maranhão). Acta Scientiarum. Biol. Sci. , v. 25, n. 2, p. 261-266, 2003.
SOUSA, O. V.; MACRAE, A.; MENEZES, F. G.; GOMES, N. C. M.; VIEIRA, R. H. S.; MENDONÇA-HAGLER, L.C.S. The impact of shrimp farming effluent on bacterial communities in mangrove waters, Ceará, Brazil. Mar. Pollut. Bull., v. 52, p. 1725-1734, 2006.
THOMPSON, F. L.; TETSUYA, I.; SWINGS, J. Biodiversity of vibrios. Microbiol. Mol. Biol. Revs, v. 68, n. 3, p. 403-431, 2004.
THOMPSON, F. L.; THOMPSON, C. C.; VICENTE, A. C. P.; THEOPHILO, G. N. D; HOFER, E. ; SWINGS, J. Genomic diversity of clinical and environmental Vibrio cholerae strains isolated in Brazil between 1991 and 2001 as revealed by fluorescent amplified fragment length polymorphism analysis. J. clin. Microbiol, v. 41, n. 5, p. 1946-1950, 2003.
TROTT, L.A.; ALONGI, D.M. The impact of shrimp pond efluent on water quality and phytoplankton biomass in a tropical mangrove estuary. Mar. Pollut. Bull., v. 40, n. 11, p. 947-951, 2000.
TROTT, L. A.; ALONGI, D. M. The impact of shrimp pond efluent on water quality and phytoplankton biomass in a tropical mangrove estuary. Mar. Pollut. Bull., v. 40, n. 11, p. 947-951, 2000.
VASEEHARAN, B.; RAFFIG HUSSIAN, M.; CHENG, J. C. RpoN gene, RAPD profile, antimicrobial resistance and plasmids of Vibrio anguillarum isolates from vibriosis infected Penaeus monodon Letts Appl. Microbiol., v. 47, n. 5, p. 380-385, 2008.
YANO, Y.; YOKOYAMA, M.; SATOMI, M.; OIKAWA, H.; CHEN, S-S. Occurrence of Vibrio vulnificus in fish and shellfish available from markets in china. J. Food Protect., , v. 67, n. 8, p. 1617-1623, 2004.

Publication Dates

Publication in this collection
03 Nov 2010
Date of issue
Sept 2010

History

Reviewed
14 May 2009
Received
19 May 2009
Accepted
09 Mar 2010

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] AGUIRRE-GUZMÁN, G.; VÁZQUEZ-JUÁREZ, R.; ASCENCIO, F. Differences in the susceptibility of American white shrimp larval substages (Litopenaeus vannamei) to four vibrio species. J Invertebr Pathol., v. 78, n. 4, p. 215-219, 2001

[2] ALAVANDI, S. V.; MANORANJITA, V.; VIJAYAN, K. K.; KALAIMANI, N.; SANTIAGO, T. C. Phenotypic and molecular typing of Vibrio harveyi isolates and their pathogenicity to tiger shrimp larvae. Letts appl. Microbiol., v. 43, n. 5, p. 566-570, 2006.

[3] ALSINA, M.; BLANCH, A. R. A set of keys for biochemical identification of environment Vibrio species. J. appl. Bact., v. 76, n. 1, p. 79-85, 1994a.

[4] ALSINA, M.; BLANCH, A. R. Improvement and update of a set of keys for biochemical identification of environmental Vibrio species. J. appl. Bact., v. 77, p. 719-721, 1994b.

[5] ALVAREZ, R. J. D.; AGURTO, C; OBREGÓN, J.; PEROZA, L. Detección de Baculovirus penaei y de casos de vibriosis em Litopenaeus vannamei and L. stylirostris en uma granja de la costa occidental de Venezuela. Revta cient. FCV-LUZ, v. 13, n. 4, p. 255-262, 2003.

[6] BARBIERI, E.; FALZANO, L.; FIORENTINI, C.; PIANETTI, A.; BAFFONE, W.; FABBRI, A.; MATARRESE, P.; CASIERE, A.; KATOULI, M.; KÜHN, I.; MÖLLBY, R.; BRUSCOLINI, F.; DONELLI, G. Occurrence, diversity, and pathogenicity of halophilic Vibrio spp. and non-O1 Vibrio cholerae from estuarine waters along the Italian Adriatic Coast. Appl. environ.Microbiol., v. 65, n. 6, p. 2748, 2753, 1999.

[7] BLACKSTONE, G. M.; NORDSTROM, J. L.; VICKERY, M. C.; BOWEN, M. D.; MEYER, R. F.; DEPAOLA, A. Detection of pathogenic Vibrio parahaemolyticus in oyster enrichments by real time PCR. J Microbiol Meth., v. 53, n. 2, p. 149-155, 2003.

[8] BLACKWELL, K. D.; OLIVER, J. D. The ecology of Vibrio vulnificus, Vibrio cholerae, and Vibrio parahaemolyticus in North Carolina estuaries. J. Microbiol., v. 46, n. 2, p. 146-153, 2008.

[9] CERVINO, J. M.; HAYES, R. L.; POLSON, S. W.; POLSON, S. C.; GOREAU, T.J.; MARTINEZ, R. J; SMITH, G. W. Relationship of Vibrio species infection and elevated temperatures to yellow blotch/band disease in Caribbean corals. Appl. environ. Microbiol., v. 70, n. 11, p. 6855-6864, 2004.

[10] CONEJERO, M.J.U.; HEDREYDA, C. T. Isolation of partial toxR gene of Vibrio harveyi and design of toxR-targeted PCR primers for species detection. J. appl. Microbiol., v. 95, n. 3, p. 602-611, 2003.

[11] DAS, B.; KHAN, Y. S.; DAS, P. Environmental impact of aquaculture-sedimentation and nutrient loadings from shrimp culture of the southeast coastal region of the Bay of Bengal. J environ. Sci., (China), v. 16, n. 3, p. 466-470, 2004.

[12] DONOVAN, T. J.; NETTEN, P. Culture media for the isolation and enumeration of pathogenic Vibrio species in food and environmental samples. Int. J. Food Microbiol., v. 26, p. 77-91, 1995.

[13] FUENZALIDA, L.; ARMIJO, L.; ZABALA, B.; HERNÁNDEZ, C.; RIOSECO, M. L.; RIQUELME, C.; ESPEJO, R. T. Vibrio parahaemolyticus strains isolated during investigation of the summer 2006 seafood related diarrhea outbreaks in two regions of Chile. Int. J. Food Microbiol., v. 117, p. 270-275, 2007.

[14] GARTHRIGHT, W. E. Appendix 2: most probable number from serial dilutions. In: FOOD AND DRUG ADMINISTRATION - FDA. Bacteriological analytical manual on line. FDA/CFSAM, 2001. Available: <http://www.cfsan.fda.gov/~edam/bam-a2.html>. Accessed: 06/09/2004.

[15] GONÇALVES, E. G. R.; LOPES, M. J. S.; OLIVEIRA, E. G.; HOFER, E. Avaliação de Vibrio cholerae com o zooplâncton de águas estuárias da Baía de São Marcos/São Luís - MA, Brasil. Rev. Soc. Bras. Med. Trop., v. 37, n. 4, p. 318-323, 2004.

[16] HEIDELBERG, J.; HEIDELBERG, K. B; COLWELL, R. R. Bacteria of the gamma-subclass Proteobacteria associated with zooplankton in Chesapeake Bay. Appl. environ. Microbiol., v. 68, p. 5498-5507, 2002.

[17] HERVIO-HEATH, D.; COLWELL, R. R.; DERRIEN, A.; ROBERT-PILLOT, A. FOURNIER, J. M.; POMMEPUY, M. Occurrence of pathogenic vibrios in coastal areas of France. J. appl. Microbiol., v. 92, p. 1123-1135, 2002.

[18] KAYSNER, C. A.; DEPAOLA JR., A. Vibrio cholerae, V. parahaemolyticus, V. vulnificus, and other Vibrio spp. 2004. Available:<http://www.cfsan.fda.gov/~ebam/bam9.html#authors> Accessed: 10/01/2006.

[19] LACERDA, L. D. Inputs of nitrogen and phosphorus to estuaries of northeastern Brazil from intensive shrimp farming. Braz. J. aquat. Sci. Technol., v. 10, n. 2, p. 13-27, 2006.

[20] LEE, J-K.; JUNG, D-W.; EOM, S-Y.; OH, S-W; KIM, Y.; KWAK, H-S.; KIM, Y-H. Occurrence of Vibrio parahaemolyticus in oysters from Korean retail outlets. Food Control, v. 19, p. 990-994, 2008.

[21] MACÍAN, M. C.; ROSA AZNAR, C. R. A.; GARAY, E. AND PUJALTE, M. J. Identification of Vibrio spp (other than V. vulnificus) recovered on CPC agar from marine natural samples. Int. Microbiol., v. 3, p. 51-53, 2000.

[22] MEIRELES, A. J. A.; VICENTE DA SILVA, E. Abordagem geomorfológica para a realização de estudos integrados para o planejamento e gestão em ambientes flúvio-marinhos. Scripta Nova Revista electrónica de geografía y ciencias sociales, Universidad de Barcelona, v. 6, n. 118, 2002.

[23] MOLITORIS, E.; JOSEPH, S. W.; KRICHEVSKY, M.; SINDHUHARDJA, W.; R. R. COLWELL. Characterization and distribution of Vibrio alginolyticus and Vibrio parahaemolyticus isolated in Indonesia. Appl. environ. Microbiol., v. 50, n. 6, p. 1388-1395, 1995.

[24] MURAKAMI, K.; FUSE, H.; TAKIMURA, O.; KAMIMURA, K.; YAMAOKA, Y. Phylogenetic analysis of marine environmental strains of Vibrio that produce aerobactin. J. mar. Biotechnol., v. 6, n. 2, p. 76-79, 1998.

[25] PÁEZ-OSUNA, F.; GUERRERO-GALVÁN, S. R.; RUIZ-FERNÁNDEZ, A. C. The environmental impact of shrimp aquaculture and the coastal pollution in Mexico. Mar. Pollut. Bull., v. 36, n. 1, p. 65-75, 1998.

[26] PÁEZ-OSUNA, F. The environmental impact of shrimp Aquaculture: Causes, effects, and mitigating alternatives. Environ. Mgmt, v. 28, n. 1, p. 131-140, 2001.

[27] PFEFFER, C. S.; HITE, M. F.; OLIVER, J. D. Ecology of Vibrio vulnificus in estuarine waters of Eastern North Carolina. . Appl. environ. Microbiol., v. 69, n. 6, p. 3526-3531, 2003.

[28] SERRA, C. L. M.; CAVALCANTE, P. R.; ALVES, L. M. C.; NASCIMENTO, A.R.; DINIZ, S.C.C.S. Avaliação de parâmetros físico-químicos e pesquisa de Vibrio parahaemolyticus em águas do estuário do rio Anil (São Luís, Estado do Maranhão). Acta Scientiarum. Biol. Sci. , v. 25, n. 2, p. 261-266, 2003.

[29] SOUSA, O. V.; MACRAE, A.; MENEZES, F. G.; GOMES, N. C. M.; VIEIRA, R. H. S.; MENDONÇA-HAGLER, L.C.S. The impact of shrimp farming effluent on bacterial communities in mangrove waters, Ceará, Brazil. Mar. Pollut. Bull., v. 52, p. 1725-1734, 2006.

[30] THOMPSON, F. L.; TETSUYA, I.; SWINGS, J. Biodiversity of vibrios. Microbiol. Mol. Biol. Revs, v. 68, n. 3, p. 403-431, 2004.

[31] THOMPSON, F. L.; THOMPSON, C. C.; VICENTE, A. C. P.; THEOPHILO, G. N. D; HOFER, E. ; SWINGS, J. Genomic diversity of clinical and environmental Vibrio cholerae strains isolated in Brazil between 1991 and 2001 as revealed by fluorescent amplified fragment length polymorphism analysis. J. clin. Microbiol, v. 41, n. 5, p. 1946-1950, 2003.

[32] TROTT, L.A.; ALONGI, D.M. The impact of shrimp pond efluent on water quality and phytoplankton biomass in a tropical mangrove estuary. Mar. Pollut. Bull., v. 40, n. 11, p. 947-951, 2000.

[33] TROTT, L. A.; ALONGI, D. M. The impact of shrimp pond efluent on water quality and phytoplankton biomass in a tropical mangrove estuary. Mar. Pollut. Bull., v. 40, n. 11, p. 947-951, 2000.

[34] VASEEHARAN, B.; RAFFIG HUSSIAN, M.; CHENG, J. C. RpoN gene, RAPD profile, antimicrobial resistance and plasmids of Vibrio anguillarum isolates from vibriosis infected Penaeus monodon Letts Appl. Microbiol., v. 47, n. 5, p. 380-385, 2008.

[35] YANO, Y.; YOKOYAMA, M.; SATOMI, M.; OIKAWA, H.; CHEN, S-S. Occurrence of Vibrio vulnificus in fish and shellfish available from markets in china. J. Food Protect., , v. 67, n. 8, p. 1617-1623, 2004.

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Quantification and distribution of vibrio species in water from an estuary in Ceará-Brazil impacted by shrimp farming

Abstracts

Publication Dates

History