Comparação in vitro da eficácia de diferentes formulações do gel de EDTA 24% no condicionamento da superfície radicular

In vitro comparison of different 24% EDTA gel formulations efficacy on root surface conditioning

Resumos

INTRODUÇÃO: A biomodificação radicular visa a alterar a superfície radicular para auxiliar no reparo das estruturas periodontais de suporte destruídas pela doença periodontal. OBJETIVO: Avaliar por meio de microscopia eletrônica de varredura a eficácia do condicionamento químico com gel de EDTA 24% na remoção de smear layer e na exposição de fibras colágenas, verificando-se diferentes marcas comerciais, modos e tempos de aplicação. MATERIAL E MÉTODO: Um total de 225 amostras foram obtidas e distribuídas aleatoriamente em cinco grupos: soro fisiológico (controle); EDTA 24% (Farmácia Santa Paula); EDTA-T 24% (Farmácia Santa Paula); EDTA 24% (PrefGel; Straumann); EDTA 24% (Biodinâmica). As amostras de cada grupo foram redistribuídas aleatoriamente em subgrupos (n = 5) e condicionadas com os diferentes géis de EDTA nos tempos de 1, 2 e 3 minutos, e nos modos de aplicação tópica, fricção vigorosa e fricção suave. Os testes de Kruskal-Wallis e Dunn (p < 0,05) foram utilizados e aplicados separadamente para os fatores: marca comercial, tempo e modo de aplicação. RESULTADO: A análise estatística revelou que o gel de EDTA 24% da Biodinâmica promoveu a menor remoção de smear layer. Não foram detectadas diferenças estatísticas para os tempos de aplicação. O modo de aplicação fricção suave resultou em menor quantidade de smear layer residual. CONCLUSÃO: Todos os géis de EDTA 24% utilizados foram eficazes na remoção de smear layer e na exposição de fibras colágenas, independentemente do tempo de aplicação; no entanto, o modo de fricção suave foi mais efetivo. Na análise comparativa entre os produtos, o EDTA da Biodinâmica foi menos efetivo, já que promoveu menor remoção de smear layer e exposição de fibras colágenas.

EDTA; colágeno; microscopia eletrônica de varredura; raiz dentária

INTRODUCTION: The main goal of root biomodification is to modify the root surface in order to improve the repair of periodontal tissues destroyed by periodontal disease. OBJECTIVE: To evaluate the conditioning efficacy of 24% EDTA gel of different trademarks, considering the variables time and application method, by scanning electron microscopy. MATERIAL AND METHOD: 225 samples were randomly assigned to five groups: sterile saline solution (control); 24% EDTA (Santa Paula Pharmacy); 24% EDTA-T (Santa Paula Pharmacy); 24% EDTA (PrefGel - Straumann); 24% EDTA (Biodinâmica). For each experimental group the gels were applied for 1, 2 or 3 minutes by topical application; vigorous or smooth friction. The Kruskal-Wallis and Dunn tests (p < 0,05) were used and individually applied to the variables commercial brand, time and type of application. RESULT: The statistical analysis showed that 24% EDTA Biodinâmica gel promoted less smear layer removal compared to the other groups. No significant differences could be detected among the different times of application. The smooth friction resulted in lower amount of residual smear layer. CONCLUSION: All 24% EDTA gels tested were able to remove smear layer and to expose dentinal tubules irrespective of application time; however, the application method by smooth friction was more effective than the other ones. The comparative analysis demonstrated that the Biodinâmica gel was the least effective product, promoting less smear layer removal and exposure of collagen fibers than the other EDTA gels.

EDTA; collagen; microscopy electron scanning; tooth root

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Datas de Publicação

• Publicação nesta coleção
  11 Mar 2013
• Data do Fascículo
  Fev 2013

Histórico

• Recebido
  08 Nov 2012
• Aceito
  19 Dez 2012