Incidência e aspectos histopatológicos da infecção por Helicobacter spp. em gatos da Cidade de Recife, Pernambuco, Brasil

Macêdo, J.S.; Mendonça, F.S.; Silva, K.R.L. da; Barros, M.E.G. de; Evêncio-Neto, J.

Resumos

O presente trabalho objetivou investigar a prevalência da bactéria Helicobacter spp. em gatos da Cidade do Recife, Pernambuco. As amostras de estômagos analisadas foram colhidas de 119 gatos sem raça e idade definida, sendo 56 machos e 63 fêmeas. Para pesquisa de Helicobacter spp., foram realizados os métodos de teste rápido da urease e exames histopatológicos. As análises pelo teste rápido da urease mostraram que em 82,35% das amostras foi detectada reação positiva para Helicobacter. Na análise histopatológica, verificou-se que em 59,66% das amostras (71/119) apresentaram lesões. Essas lesões consistiram em infiltrado linfoplasmocitário com folículo linfoide, microabcessos, necrose, hipotrofia; em 40,34% das amostras (48/119) não foram observadas alterações histológicas. Conclui-se que gatos oriundos da Cidade de Recife, Pernambuco< apresentam alta incidência de infecção por Helicobacter spp. Infiltrado difuso de células linfoplasmocitárias foram as alterações histopatológicas mais frequentes observadas na mucosa gástrica de gatos infectados por Helicobacter spp.

Helicobacteriose; urease; gastrite; bactéria; felinos

The present study aimed to investigate the incidence of the bacterium Helicobacter spp. in cats from Recife, Pernambuco State, Brazil. Stomach samples were collected from 119 cats, without distinction between race or age, the breakdown by sex being 56 males and 63 females. The urease quick test and histopathological examination using both hematoxilin-eosin (HE) and Giemsa stain were performed. The urease quick test showed positive results for Helicobacter in 82.35% of the samples. The histopathology revealed an alteration in 59.66% (71/119) of the samples, such as lymphoplasmocitic infiltration with a lymphoid foliculum, microabscess, necrosis, and hypotrophy, while in 40.34% of the samples (48/119) no alterations were observed. It was concluded that the cats from the city of Recife, State of Pernambuco, Brazil, presented a high incidence of infection with Helicobacter spp. Diffuse infiltrate of lymphocytes cells was the most frequent histopathological change observed in the gastric mucosa of cats infected with Helicobacter spp.

Helicobacteriosis; urease; gastritis; bacterium; cats

AVENAUD, P.; MARAIS, A.; MONTEIRO, L.; LE BAIL, B.; BIOLAC SAGE, P.; BALABAUD, CH.; MÉGRAUD, F. Detection of Helicobacter species in the liver of patients with and without primary liver carcinoma. Cancer, v.89, p.1431-1439, 2000.
BARROS, C.S.L.; MARQUES, G.H.F. Procedimentos para diagnóstico das doenças do sistema nervoso central de bovinos. Brasília: Departamento de Defesa Animal, MAPA, 2003. p.50.
BIZZOZERO, G. Sulla presenza di bateri nelle ghiandole gastriche del cane (in itlaian). Atti della Reale Accademia delle Scienze di Torino, v.28, p.249, 1893.
BLASER, M.J. Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. Journal of Infectous Diseases, v.33, p.626, 1990.
BLASER, M.J.; PEREZ-PEREZ, G.I.; KLEANTHOUS, H.; COVER, T.L.; PEEK, R.M., CHYOU, P.H.; STEMMERMANN, G.N.; NOMURA, A. Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Research, v.55, p.2111-2115, 1995.
BOGLIOLO, L. Patologia. 7.ed. Rio de Janeiro: Guanabara Koogan, 2006.
BOYANOVA, L.; DEREJIAN, S.; KOUMANOVA, R.; KATSAROV, N.; GERGOVA, G.; MITOV, I.; NIKOLOV, R.; KRASTEV, Z. Inhibition of Helicobacter pylori growth in vitro by Bulgarian propolis: preliminary report. Journal of Medical Microbiology, v.52, n.5, p.417-419, 2003.
CASTELLOTE, J.; GUARDIOLA, J.; PORTA, F.; FALCÓ, A. Rapid uréase test: effect of preimmersion of biopsy forceps in formalin. Gastrointestinal Endoscopy, v.53, n.6, p.744, 2001.
CASTRO, L.P. COELHO, L.G.V.; BARBOSA, A.J.A.; VIEIRA, W.L.S. Helicobacter pylori e afeccções associadas. Jornal Brasileiro de Gastroenterologia, v.2, n.3, p.19-30, 2003.
CATTOLI, G.; BART, A.; KLAVER, P.S.J.; ROBIJN, R.J.; BEUMER, H.J.; VAN VUGT, R.; POT, R.G.J.; VAN DER GAGG, I.; VANDENBROUCKEGRAULS, C.M.J.E.; COVER, T.L.; BLASER, M.J. Helicobacter pylori: a bacterial cause of gastritis, peptic ulcer disease, and gastric cancer. AMS News, v.61, n.5, p.21-26, 1995.
DANESH, J.; YOUNGMAN, L.; CLARK, S.; PARISH, S.; PETO, R.; COLLINS, R. Helicobacter pylori infection and early onset myocardial infarctin: case-control and sibling pairs study. BMJ: British Medical Journal, v.319, n.7218, p.1157-1162, 1999.
EATON, K.A.; DEWHIRST, F.E.; PASTER, B.J.; TZELLAS, N.; COLEMAN, B.E.; PAOLA, J.; SHERDING, R. Prevalence and varieties of Helicobacter species in dogs from random sources and pet dogs: animal and public health implications. Journal of Clinical Microbiology, v.34, n.12, p.3165-3170, 1996.
FOX, J.G.; BATCHELDER, M.; MARINI, R.; YAN, L.; HANDT, L. SHAMES, B.; HAYWARD, A.; CAMPBELL, J.; MURPHY, J.C. Helicobacter pylori-induced gastritis in the domestic cat. Infection and Immunity, v.63, n.7, p.2674-2681, 1995.
GASBARRINI, A.; FRANCESCHI, F. Autoimmune diseases and Helicobacter pylori infection. Biomedicine & Pharmacotherapy, v.53, n.6, p.223, 1999.
HANDT, L.K.; FOX, J.G.; DEWHIRST, F.E.; FRASER, G.J.; PASTER, B.J.; YAN, L.L.; ROSMIAREK, H.; RUFO, R.; STALIS, I.H. Characterization of Feline Helicobacter pylori Strains and Associated Gastritis in a Colony of Domestic Cats. Journal of Clinical Microbiology, v.33, p.2280-2289, 1995.
HANDT, L.K.; FOX, J.G.; STALIS, I.H.; RUFP, R.; LEE, G.; LINN, J. Helicobacter pylori Isolated from the Domestic Cat: public health implications. Infection and Immunity, v.62. n.6, p.2367-2374, 1994.
HERMANNS, W.; KREGEL, K.; BREUNER, W.; LECHNER, L. Helicobacterlike organisms: histopathological examination of gastric biopsies from dogs and cats. Journal of Comparative Pathology, v.112, p.307-18, 1995.
HIYAMA, T.; HARUMA, K.; KITADAI, Y.; MIYAMOTO, M.; TANAKA, S.; YOSHIHARA, M.; et al. B-Cell monoclonality in Helicobacter pylori-associated chronic atrophic gastritis. Virchows Archiv, v.438, p.232-237, 2001.
JALAVA, K.; ON, S.L.; VANDAMME, P.A.; HAPPONEN, I.; SUKURA, A.; HÄNNINEN, M. L. Isolation and Identification of Helicobacter spp. from Canine and Feline Gastric mucosa. Applied and Environmental Microbiology, v.64, n.10, p.3998-4006, 1998.
LANGENBERG, W., RAUWS, E.A., OUDBIER, J.H., TYTGAT, G.N.J.. Patient-to-patient transmission of Campylobacter pylori infection by fiberoptic gastroduodenoscopy and biopsy. Journal of Infectious Disease, v.161, p.507–511, 1990.
MARSHAL, B.J.; WARREN, J.R. Unidentified curved bacilli of gastric epithelium in active chronic gastritis. Lancet, v.1, n.5, p.1273, 1983.
MICHALANY, J. Técnica histológica em anatomia patológica: com instruções para o cirurgião, enfermeira e citotécnico. São Paulo: EPU, 1980. 277p.
MITCHELL, H.; ENGLISH, D.R.; ELLIOTT, F.. Immunoblotting using multiple antigens is essential to demonstrate the true risk of Helicobacter pylori infection for gastric cancer. Alimentary Pharmacology & Therapeutics, v.28, p.903–910, 2006.
MORGNER, A. Malignant tumors of the stomach. Gastric mucosa-associated lymphoid tissue lymphoma and Helicobacter pylori. Gastroenterology Clinics of North America, v.29, n.3, p.593-607, 2000.
MORGNER, A.; LEHN, N.; ANDERSEN, L.P.; THIEDE, C.; BENNEDSEN, M.; TREBESIUS, K.; NEUBAUER, B.; NEUBAUER, A.; STLTE, M., BAYERDÖRFER, E. Helicobacter heilmannii - associated primary gastric low-grade MALT lymphoma: complete remission after curing the infection. Gastroenterology, v.118, p.821-828, 2000.
NEIGER, R.A. Detection and prevalence of Helicobacter infection in pet cats. Journal of Clinical Microbilogy, v.36, n.3, p.634-637, 1998.
NELSON, R.W.; COUTO, C.G. Leucopenia e Leucocitose. In: ___________________ (Ed.). Medicina interna de pequenos animais. 3.ed. Rio de Janeiro: Elsevier, 2006. p.1187-1190.
NILSSON, H.O.; TANEERA, J.; CAASTEDAL, M.; GLATZ, E.; OLSSON, R.; WASDTRÖM, T. Identification of H. pylori and other Helicobacter species by PCR, hybridization, and particial DNA sequencing in human liver samples from patients with primary sclerosing cholangitis or primary biliary cirrhosis. Journal of Clinical Microbiology, v.38, p.1072-1076, 2000.
NORRIS, C.R.; MARKS, S.L.; EATON, K.A.; TORABIAN, S.Z.; MUNN, R.J.; SOLNICK, J.V. Healthy cats are commonly with "Helicobacter heilmannii" that is associated with minimal gastritis. Journal of Clinical Microbiology, v.37, n.1, p.189-194, 1999.
OTTO, G.; HAZELL, S.H.; FOX, J.G.; HOWLETT, C.R.; MURPHY, J.C.; O'ROURKE, J.L.; LEE, A. Animal and public health implications of gastric colonization of cats by Helicobacter-like organisms. Journal of Clinical Microbiology, v.32, n.4, p.1043-1049, 1994.
QUEIROZ, D.M.; MENDES, E.N.; ROCHA, G.A.; OLIVEIRA, A.M.; OLIVEIRA, C.A.; MAGALHAES, P.P.; MOURA, S.B.; NOGUEIRA, A.M. cagA-positive Helicobacter pylori and risk for developing gastric carcinoma in Brazil. International Journal of Cancer, p.135-139, 1998.
RADIN, M.J.; EATON, K.A.; KRAKOWKA, S.; MORGAN, D.R.; LEE, A.; OTTO, G.; FOX, J. Helicobacter pylori astric infection in gnotobiotic beagle dogs. Infection and Immunology, v.58, n.8, p.2606-2612, 1990.
SOUTO, F.J.D.; FONTES, C.J.F.; ROCHA, G.A.; OLIVEIRA, A.M.R.; MENDES, E.N.; QUEIROZ, D.M.M. Q. Prevalence of Helicobacter pylori Infection in a rural área of the State of Mato Grosso, Brazil. Memórias do Instituto Oswaldo Cruz, v.4, p.93-171, 1998.
TAMS, T.R. Gastroenterologia de pequenos animais São Paulo: Rocca, 2005.
TUCCI, A.; POLI, L.; BIASCO, G.; PAPARO, G.F.; TOSETTI, C.; FUSAROLI, P.; SAMBRI, V.; DONATI, M.; GRIGIONI, W.; LABATE, A.M.; STANGHELLINI, V.; CALETTI, G. Helicobacter pylori infection and gastric function in patientes with fundic atrophic gastritis.Digestive Diseases and Sciences, v.46, n.7, p.1573-1583, 2001.
VALLE, C.; BIZINELL, S.L.; Prevalência do Helicobacter pylori em pacientes submetidos a endoscopia digestiva Revista Médica do Paraná, v.50, p.17-20, 1993.
WILLIAMS, C.L. Helicobacter pylori and endoscopy.Journal of Hospital Infection, v.41, n.4, p.263-268, 1999.

Datas de Publicação

Publicação nesta coleção
03 Jun 2013
Data do Fascículo
Dez 2012

Histórico

Recebido
26 Abr 2011
Aceito
24 Jul 2012

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] AVENAUD, P.; MARAIS, A.; MONTEIRO, L.; LE BAIL, B.; BIOLAC SAGE, P.; BALABAUD, CH.; MÉGRAUD, F. Detection of Helicobacter species in the liver of patients with and without primary liver carcinoma. Cancer, v.89, p.1431-1439, 2000.

[2] BARROS, C.S.L.; MARQUES, G.H.F. Procedimentos para diagnóstico das doenças do sistema nervoso central de bovinos. Brasília: Departamento de Defesa Animal, MAPA, 2003. p.50.

[3] BIZZOZERO, G. Sulla presenza di bateri nelle ghiandole gastriche del cane (in itlaian). Atti della Reale Accademia delle Scienze di Torino, v.28, p.249, 1893.

[4] BLASER, M.J. Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. Journal of Infectous Diseases, v.33, p.626, 1990.

[5] BLASER, M.J.; PEREZ-PEREZ, G.I.; KLEANTHOUS, H.; COVER, T.L.; PEEK, R.M., CHYOU, P.H.; STEMMERMANN, G.N.; NOMURA, A. Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Research, v.55, p.2111-2115, 1995.

[6] BOGLIOLO, L. Patologia. 7.ed. Rio de Janeiro: Guanabara Koogan, 2006.

[7] BOYANOVA, L.; DEREJIAN, S.; KOUMANOVA, R.; KATSAROV, N.; GERGOVA, G.; MITOV, I.; NIKOLOV, R.; KRASTEV, Z. Inhibition of Helicobacter pylori growth in vitro by Bulgarian propolis: preliminary report. Journal of Medical Microbiology, v.52, n.5, p.417-419, 2003.

[8] CASTELLOTE, J.; GUARDIOLA, J.; PORTA, F.; FALCÓ, A. Rapid uréase test: effect of preimmersion of biopsy forceps in formalin. Gastrointestinal Endoscopy, v.53, n.6, p.744, 2001.

[9] CASTRO, L.P. COELHO, L.G.V.; BARBOSA, A.J.A.; VIEIRA, W.L.S. Helicobacter pylori e afeccções associadas. Jornal Brasileiro de Gastroenterologia, v.2, n.3, p.19-30, 2003.

[10] CATTOLI, G.; BART, A.; KLAVER, P.S.J.; ROBIJN, R.J.; BEUMER, H.J.; VAN VUGT, R.; POT, R.G.J.; VAN DER GAGG, I.; VANDENBROUCKEGRAULS, C.M.J.E.; COVER, T.L.; BLASER, M.J. Helicobacter pylori: a bacterial cause of gastritis, peptic ulcer disease, and gastric cancer. AMS News, v.61, n.5, p.21-26, 1995.

[11] DANESH, J.; YOUNGMAN, L.; CLARK, S.; PARISH, S.; PETO, R.; COLLINS, R. Helicobacter pylori infection and early onset myocardial infarctin: case-control and sibling pairs study. BMJ: British Medical Journal, v.319, n.7218, p.1157-1162, 1999.

[12] EATON, K.A.; DEWHIRST, F.E.; PASTER, B.J.; TZELLAS, N.; COLEMAN, B.E.; PAOLA, J.; SHERDING, R. Prevalence and varieties of Helicobacter species in dogs from random sources and pet dogs: animal and public health implications. Journal of Clinical Microbiology, v.34, n.12, p.3165-3170, 1996.

[13] FOX, J.G.; BATCHELDER, M.; MARINI, R.; YAN, L.; HANDT, L. SHAMES, B.; HAYWARD, A.; CAMPBELL, J.; MURPHY, J.C. Helicobacter pylori-induced gastritis in the domestic cat. Infection and Immunity, v.63, n.7, p.2674-2681, 1995.

[14] GASBARRINI, A.; FRANCESCHI, F. Autoimmune diseases and Helicobacter pylori infection. Biomedicine & Pharmacotherapy, v.53, n.6, p.223, 1999.

[15] HANDT, L.K.; FOX, J.G.; DEWHIRST, F.E.; FRASER, G.J.; PASTER, B.J.; YAN, L.L.; ROSMIAREK, H.; RUFO, R.; STALIS, I.H. Characterization of Feline Helicobacter pylori Strains and Associated Gastritis in a Colony of Domestic Cats. Journal of Clinical Microbiology, v.33, p.2280-2289, 1995.

[16] HANDT, L.K.; FOX, J.G.; STALIS, I.H.; RUFP, R.; LEE, G.; LINN, J. Helicobacter pylori Isolated from the Domestic Cat: public health implications. Infection and Immunity, v.62. n.6, p.2367-2374, 1994.

[17] HERMANNS, W.; KREGEL, K.; BREUNER, W.; LECHNER, L. Helicobacterlike organisms: histopathological examination of gastric biopsies from dogs and cats. Journal of Comparative Pathology, v.112, p.307-18, 1995.

[18] HIYAMA, T.; HARUMA, K.; KITADAI, Y.; MIYAMOTO, M.; TANAKA, S.; YOSHIHARA, M.; et al. B-Cell monoclonality in Helicobacter pylori-associated chronic atrophic gastritis. Virchows Archiv, v.438, p.232-237, 2001.

[19] JALAVA, K.; ON, S.L.; VANDAMME, P.A.; HAPPONEN, I.; SUKURA, A.; HÄNNINEN, M. L. Isolation and Identification of Helicobacter spp. from Canine and Feline Gastric mucosa. Applied and Environmental Microbiology, v.64, n.10, p.3998-4006, 1998.

[20] LANGENBERG, W., RAUWS, E.A., OUDBIER, J.H., TYTGAT, G.N.J.. Patient-to-patient transmission of Campylobacter pylori infection by fiberoptic gastroduodenoscopy and biopsy. Journal of Infectious Disease, v.161, p.507–511, 1990.

[21] MARSHAL, B.J.; WARREN, J.R. Unidentified curved bacilli of gastric epithelium in active chronic gastritis. Lancet, v.1, n.5, p.1273, 1983.

[22] MICHALANY, J. Técnica histológica em anatomia patológica: com instruções para o cirurgião, enfermeira e citotécnico. São Paulo: EPU, 1980. 277p.

[23] MITCHELL, H.; ENGLISH, D.R.; ELLIOTT, F.. Immunoblotting using multiple antigens is essential to demonstrate the true risk of Helicobacter pylori infection for gastric cancer. Alimentary Pharmacology & Therapeutics, v.28, p.903–910, 2006.

[24] MORGNER, A. Malignant tumors of the stomach. Gastric mucosa-associated lymphoid tissue lymphoma and Helicobacter pylori. Gastroenterology Clinics of North America, v.29, n.3, p.593-607, 2000.

[25] MORGNER, A.; LEHN, N.; ANDERSEN, L.P.; THIEDE, C.; BENNEDSEN, M.; TREBESIUS, K.; NEUBAUER, B.; NEUBAUER, A.; STLTE, M., BAYERDÖRFER, E. Helicobacter heilmannii - associated primary gastric low-grade MALT lymphoma: complete remission after curing the infection. Gastroenterology, v.118, p.821-828, 2000.

[26] NEIGER, R.A. Detection and prevalence of Helicobacter infection in pet cats. Journal of Clinical Microbilogy, v.36, n.3, p.634-637, 1998.

[27] NELSON, R.W.; COUTO, C.G. Leucopenia e Leucocitose. In: ___________________ (Ed.). Medicina interna de pequenos animais. 3.ed. Rio de Janeiro: Elsevier, 2006. p.1187-1190.

[28] NILSSON, H.O.; TANEERA, J.; CAASTEDAL, M.; GLATZ, E.; OLSSON, R.; WASDTRÖM, T. Identification of H. pylori and other Helicobacter species by PCR, hybridization, and particial DNA sequencing in human liver samples from patients with primary sclerosing cholangitis or primary biliary cirrhosis. Journal of Clinical Microbiology, v.38, p.1072-1076, 2000.

[29] NORRIS, C.R.; MARKS, S.L.; EATON, K.A.; TORABIAN, S.Z.; MUNN, R.J.; SOLNICK, J.V. Healthy cats are commonly with "Helicobacter heilmannii" that is associated with minimal gastritis. Journal of Clinical Microbiology, v.37, n.1, p.189-194, 1999.

[30] OTTO, G.; HAZELL, S.H.; FOX, J.G.; HOWLETT, C.R.; MURPHY, J.C.; O'ROURKE, J.L.; LEE, A. Animal and public health implications of gastric colonization of cats by Helicobacter-like organisms. Journal of Clinical Microbiology, v.32, n.4, p.1043-1049, 1994.

[31] QUEIROZ, D.M.; MENDES, E.N.; ROCHA, G.A.; OLIVEIRA, A.M.; OLIVEIRA, C.A.; MAGALHAES, P.P.; MOURA, S.B.; NOGUEIRA, A.M. cagA-positive Helicobacter pylori and risk for developing gastric carcinoma in Brazil. International Journal of Cancer, p.135-139, 1998.

[32] RADIN, M.J.; EATON, K.A.; KRAKOWKA, S.; MORGAN, D.R.; LEE, A.; OTTO, G.; FOX, J. Helicobacter pylori astric infection in gnotobiotic beagle dogs. Infection and Immunology, v.58, n.8, p.2606-2612, 1990.

[33] SOUTO, F.J.D.; FONTES, C.J.F.; ROCHA, G.A.; OLIVEIRA, A.M.R.; MENDES, E.N.; QUEIROZ, D.M.M. Q. Prevalence of Helicobacter pylori Infection in a rural área of the State of Mato Grosso, Brazil. Memórias do Instituto Oswaldo Cruz, v.4, p.93-171, 1998.

[34] TAMS, T.R. Gastroenterologia de pequenos animais São Paulo: Rocca, 2005.

[35] TUCCI, A.; POLI, L.; BIASCO, G.; PAPARO, G.F.; TOSETTI, C.; FUSAROLI, P.; SAMBRI, V.; DONATI, M.; GRIGIONI, W.; LABATE, A.M.; STANGHELLINI, V.; CALETTI, G. Helicobacter pylori infection and gastric function in patientes with fundic atrophic gastritis.Digestive Diseases and Sciences, v.46, n.7, p.1573-1583, 2001.

[36] VALLE, C.; BIZINELL, S.L.; Prevalência do Helicobacter pylori em pacientes submetidos a endoscopia digestiva Revista Médica do Paraná, v.50, p.17-20, 1993.

[37] WILLIAMS, C.L. Helicobacter pylori and endoscopy.Journal of Hospital Infection, v.41, n.4, p.263-268, 1999.

Brasil

Brasil

Incidência e aspectos histopatológicos da infecção por Helicobacter spp. em gatos da Cidade de Recife, Pernambuco, Brasil

Incidence and pathological aspects of infection by Helicobacter spp. In cats from the city of Recife, Pernambuco, Brazil

Resumos

Datas de Publicação

Histórico