Hibridização in situ fluorescente: princípios básicos e perspectivas para o diagnóstico de doenças infecciosas em medicina veterinária

Neves, S.M.N.; Guedes, R.M.C.

Resumos

Nesse manuscrito são discutidos aspectos relevantes sobre desenvolvimento da técnica de hibridização fluorescente in situ, seus princípios básicos, aplicações e perspectivas em medicina veterinária. Além disso, compara as vantagens e desvantagens em relação às outras técnicas de diagnóstico in situ. A FISH demonstra ser uma técnica com grande potencialidade de uso rotineiro, pois associa agilidade de execução, alta sensibilidade e especificidade e visualização do agente infeccioso viável no tecido.

Diagnóstico in situ; sonda; rRNA; fluorescência

The present study presents a review of the development of the fluorescent in situ hybridization technique, its basic principles, applications and perspectives in veterinary medicine. It furthermore compares the advantages and disadvantages in relation to other in situ diagnostic techniques. The FISH technique proves to be one with great potential for routine use insofar as it offers speed coupled with high sensitivity and specificity, while providing for the visualization of the infectious agent in tissue.

in situ diagnosis; probe; rRNA; fluorescence

AMANN, R.; FUCHS, B.M. Single-cell identification by improved FISH. Nature Reviews. Microbiology, v.6, p.339-348, 2008. doi: 10.1038/nrmicro1888.
AMANN, R.; FUCHS, B.M.; BEHRENS, S. The identification of microorganisms by fluorescence in situ hybridisation. Current Opinion in Biotechnology, v.12, n.3, p.231–236, 2001.
BAUMAN, J.G.J.; WIEGANT, J.; BORST, P.; DUIJN, P. A new method for microscopial localization of specific DNA sequences by in situ hybridization of fluorchromelabelled RNA. Experimental Cell Research, v.128, p.485-490, 1980.
BOTARI, B.; ERCOLINI, D.; GATTI, M.; NEVIANI, E. Application of FISH technology for microbiological. Applied Microbiology and Biotechnology, v.73, p.485-494, 2006. doi: 10.1007/s00253-006-0615-z.
BOUTRUP, T.S.; BOESEN, H.T.; BOYE, M.; AGERHOLM, J.S.; JENSEN, T.K. Early Pathogenesis in Porcine Proliferative Enteropathy caused by Lawsonia intracellularis Journal of Comparative Pathology, v.143, p.101-109, 2010. doi: 10.1016/j .jcpa.2010.01.006.
BOYE, M.; JENSEN, T.K.; MØLLER, K.; LESER, T.D.; JORSAL, S.E. Specific detection of the genus Serpulina, S. hyodysenteriae and S. pilosicoli in porcine intestines by fluorescent rRNA in situ hybridization. Molecular and Cellular Probes, v.12, p.323-330, 1998.
BOYE, M.; AALBAEK, B.; AGERHOLM, J.S. Fusobacterium necrophorum determined as abortifacient in sheep by laser capture microdissection and fluorescence in situ hybridization. Molecular and Cellular Probes, v.20, p.330-336, 2006.
CULLANDER, C. Fluorescent Probes for Confocal Microscopy. Methods in Molecular Biology, v.122, p.59-73, 1999.
DURAND, S.; MURPHY, C.; ZHANG, Z.; ALEXANDERSEN, S. Epithelial Distribution and Replication of Foot-and-Mouth Disease Virus RNA in Infected Pigs. Journal of Comparative Pathology, v.139, p.86-96, 2008. doi: 10.1016/j.jc pa.2008.05.004
GALL, J.G.; PARDUE, M.L. Formation and detection of RNADNA hybrid molecules in cytological preparations. Proceedings of the National Academy of Sciences of the United States of America, v.63, p.378-383, 1969.
GOOKIN, J.L.; STONE, M.R.; YAEGER, M.J.; MEYERHOLZ, D.K.; MOISAN, P. Fluorescence in situ hybridization for identification of Tritrichomonas foetus in formalin-fixed and paraffin-embedded histological specimens of intestinal trichomonosis. Veterinary Parasitology, v.172, p.139-143, 2010. doi: 10.1016/j.vetpar.2010.04.014.
HANSEN, M.S.; RODOLAKIS, A.; COCHONNEAU, D.; AGGER, J.F.; CHRISTOFFERSEN, A.; JENSEN, T.K.; AGERHOLM, J.S. Coxiella burnetii associated placental lesions and infection level in parturient cows. The Veterinary Journal, v.190, n.2, p.e135-e139, 2011. doi: 10.1 016/j.tvjl. 2010.12. 021.
JENSEN, T.K.; BOESEN, H.T.; VIGRE, H.; BOYE, M. Detection of La wsonia intracellularis in For malin-fixed Porcine Intestinal Tissue Samples: Comparison of Immunofluorescence and In-situ Hybridization, and Evaluation of the Ef fects of Controlled Autolysis. Journal of Comparative Pathology v.142, p.1-8, 2010a. doi: 10.1016/j .jcpa.2009.04.001.
JENSEN, T.K.; CHRISTENSEN, A.S.; BOYE, M. Brachyspira murdochii Colitis in Pigs. Veterinary Pathology v.47, n.2, p.334-338, 2010b.
LAKATOSOVÁ, M.; HOLECKOVÁ, B. Fluorescence in situ hybridisation. Biologia – Section Cellular and Molecular Biology, v.62, n.3, p.243-250, 2007. doi: 10.2478/s11756-007-0043-2.
LEFMANN, M.; SCHWEICKERT, B.; BUCHHOLZ, P.; GOBEL, U.; ULRICHS, T.; SEILER, P.; THEEGARTEN, D.; MOTER, A. Evaluation of Peptide Nucleic Acid-Fluorescence In Situ Hybridization for Identification of Clinically Relevant Mycobacteria in Clinical Specimens and Tissue Sections. Journal of Clinical Microbiology, v.40, n.10, p.3760-3767, 2006. doi: 10.1128/JCM.01435-06.
LEVSKY, J.M.; SINGER, R.H. Fluorescence in situ hybridization: past, present and Future. Journal of Cell Science, v.116, p.2833-2838, 2003. doi: 10.1242/jcs. 00633.
MOTER, A.; GOBEL, U.B. Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms. Journal of Microbiological Methods, v.41, p.85-112, 2000.
NORDHOFF, M.; RUHE, B.; KELLERMEIER, C.; MOTER, A.; SCHMITZ, R.; BRUNNBERG, L.; WIELER, L.H. Association of Treponema spp. with canine periodontitis. Veterinary Microbiology, v.127, p.334-342, 2008. doi: 10.1016/j.vetmic.2007.09.011.
PLOEG, M. Cytochemical nucleic acid research during the twentieth century. European Journal of Histochemistry, v.44, p.7-42, 2000.
PORS, S.E.; HANSEN, M.S.; BISGAARD, M.; JENSEN, H.E. Occurrence and associated lesions of Pasteurella multocida in porcine bronchopneumonia. Veterinary Microbiology, v.150, p.160–166, 2011a. doi: 10.1016/j.vetmic.2011.01.005.
PORS, S.E.; CHADFIELD, M.S.; SØRENSEN, D.B.; OFFENBERG, H.; HEEGAARD, P.M.H.; BISGAARD, M.; JENSEN, H.E. Pathology, Tissue Metalloproteinase Transcription and Haptoglobin Responses in Mice after Experimental Challenge with Different Isolates of Pasteurella multocida Obtained from Cases of Porcine Pneumonia. Journal of Comparative Pathology, v.145, n.2/3, p.251-260, 2011b. doi: 10.1016/j.jcpa.2011.01.002.
RUDKIN, G.T.; STOLAR, B.D. High resolution detection of DNA-RNA hybrids in situ by indirect immunofluorescence. Nature, v.265, n.3, 1977.
SUI, W.; OU, M.; CHEN, J.; WAN, Y.; PENG, H.; QI, M.; HUANG, H.; DAI, Y. Comparison of immuno histochemistry and fluorescence in situ hybridization assessment for Her-2 status in breast cancer. World Journal of Surgical Oncology, v.7, p.83, 2009.
SWINGER R.R.; TUCKER J.D. Fluorescence in situ hybridization: a brief review. Environmental and Molecular Mutagenesis, v.27, p.245-254, 1996.
TANKE, H.J.; DIRKS, R.W.; RAAP, T. FISH and immunocytochemistry: towards visualising single target molecules in living cells. Current Opinion in Biotechnology, v.16, p.49-54, 2005. doi: 10.1016/j.copbio.2004.12.001.
VOLPI, E.V.; BRIDGER, J.M. FISH glossary: an overview of the fluorescence in situ hybridization technique. BioTechniques, v.45, p.385-409, 2008. doi: 10.2144/000112811.
WOLCOT, M.J. Advances in Nucleic Acid-Based Detection Methods. Clinical Microbiology Reviews, v.5, n.4, p.370-386, 1992.

Datas de Publicação

Publicação nesta coleção
03 Jun 2013
Data do Fascículo
Dez 2012

Histórico

Recebido
21 Jul 2011
Aceito
18 Jul 2012

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] AMANN, R.; FUCHS, B.M. Single-cell identification by improved FISH. Nature Reviews. Microbiology, v.6, p.339-348, 2008. doi: 10.1038/nrmicro1888.

[2] AMANN, R.; FUCHS, B.M.; BEHRENS, S. The identification of microorganisms by fluorescence in situ hybridisation. Current Opinion in Biotechnology, v.12, n.3, p.231–236, 2001.

[3] BAUMAN, J.G.J.; WIEGANT, J.; BORST, P.; DUIJN, P. A new method for microscopial localization of specific DNA sequences by in situ hybridization of fluorchromelabelled RNA. Experimental Cell Research, v.128, p.485-490, 1980.

[4] BOTARI, B.; ERCOLINI, D.; GATTI, M.; NEVIANI, E. Application of FISH technology for microbiological. Applied Microbiology and Biotechnology, v.73, p.485-494, 2006. doi: 10.1007/s00253-006-0615-z.

[5] BOUTRUP, T.S.; BOESEN, H.T.; BOYE, M.; AGERHOLM, J.S.; JENSEN, T.K. Early Pathogenesis in Porcine Proliferative Enteropathy caused by Lawsonia intracellularis Journal of Comparative Pathology, v.143, p.101-109, 2010. doi: 10.1016/j .jcpa.2010.01.006.

[6] BOYE, M.; JENSEN, T.K.; MØLLER, K.; LESER, T.D.; JORSAL, S.E. Specific detection of the genus Serpulina, S. hyodysenteriae and S. pilosicoli in porcine intestines by fluorescent rRNA in situ hybridization. Molecular and Cellular Probes, v.12, p.323-330, 1998.

[7] BOYE, M.; AALBAEK, B.; AGERHOLM, J.S. Fusobacterium necrophorum determined as abortifacient in sheep by laser capture microdissection and fluorescence in situ hybridization. Molecular and Cellular Probes, v.20, p.330-336, 2006.

[8] CULLANDER, C. Fluorescent Probes for Confocal Microscopy. Methods in Molecular Biology, v.122, p.59-73, 1999.

[9] DURAND, S.; MURPHY, C.; ZHANG, Z.; ALEXANDERSEN, S. Epithelial Distribution and Replication of Foot-and-Mouth Disease Virus RNA in Infected Pigs. Journal of Comparative Pathology, v.139, p.86-96, 2008. doi: 10.1016/j.jc pa.2008.05.004

[10] GALL, J.G.; PARDUE, M.L. Formation and detection of RNADNA hybrid molecules in cytological preparations. Proceedings of the National Academy of Sciences of the United States of America, v.63, p.378-383, 1969.

[11] GOOKIN, J.L.; STONE, M.R.; YAEGER, M.J.; MEYERHOLZ, D.K.; MOISAN, P. Fluorescence in situ hybridization for identification of Tritrichomonas foetus in formalin-fixed and paraffin-embedded histological specimens of intestinal trichomonosis. Veterinary Parasitology, v.172, p.139-143, 2010. doi: 10.1016/j.vetpar.2010.04.014.

[12] HANSEN, M.S.; RODOLAKIS, A.; COCHONNEAU, D.; AGGER, J.F.; CHRISTOFFERSEN, A.; JENSEN, T.K.; AGERHOLM, J.S. Coxiella burnetii associated placental lesions and infection level in parturient cows. The Veterinary Journal, v.190, n.2, p.e135-e139, 2011. doi: 10.1 016/j.tvjl. 2010.12. 021.

[13] JENSEN, T.K.; BOESEN, H.T.; VIGRE, H.; BOYE, M. Detection of La wsonia intracellularis in For malin-fixed Porcine Intestinal Tissue Samples: Comparison of Immunofluorescence and In-situ Hybridization, and Evaluation of the Ef fects of Controlled Autolysis. Journal of Comparative Pathology v.142, p.1-8, 2010a. doi: 10.1016/j .jcpa.2009.04.001.

[14] JENSEN, T.K.; CHRISTENSEN, A.S.; BOYE, M. Brachyspira murdochii Colitis in Pigs. Veterinary Pathology v.47, n.2, p.334-338, 2010b.

[15] LAKATOSOVÁ, M.; HOLECKOVÁ, B. Fluorescence in situ hybridisation. Biologia – Section Cellular and Molecular Biology, v.62, n.3, p.243-250, 2007. doi: 10.2478/s11756-007-0043-2.

[16] LEFMANN, M.; SCHWEICKERT, B.; BUCHHOLZ, P.; GOBEL, U.; ULRICHS, T.; SEILER, P.; THEEGARTEN, D.; MOTER, A. Evaluation of Peptide Nucleic Acid-Fluorescence In Situ Hybridization for Identification of Clinically Relevant Mycobacteria in Clinical Specimens and Tissue Sections. Journal of Clinical Microbiology, v.40, n.10, p.3760-3767, 2006. doi: 10.1128/JCM.01435-06.

[17] LEVSKY, J.M.; SINGER, R.H. Fluorescence in situ hybridization: past, present and Future. Journal of Cell Science, v.116, p.2833-2838, 2003. doi: 10.1242/jcs. 00633.

[18] MOTER, A.; GOBEL, U.B. Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms. Journal of Microbiological Methods, v.41, p.85-112, 2000.

[19] NORDHOFF, M.; RUHE, B.; KELLERMEIER, C.; MOTER, A.; SCHMITZ, R.; BRUNNBERG, L.; WIELER, L.H. Association of Treponema spp. with canine periodontitis. Veterinary Microbiology, v.127, p.334-342, 2008. doi: 10.1016/j.vetmic.2007.09.011.

[20] PLOEG, M. Cytochemical nucleic acid research during the twentieth century. European Journal of Histochemistry, v.44, p.7-42, 2000.

[21] PORS, S.E.; HANSEN, M.S.; BISGAARD, M.; JENSEN, H.E. Occurrence and associated lesions of Pasteurella multocida in porcine bronchopneumonia. Veterinary Microbiology, v.150, p.160–166, 2011a. doi: 10.1016/j.vetmic.2011.01.005.

[22] PORS, S.E.; CHADFIELD, M.S.; SØRENSEN, D.B.; OFFENBERG, H.; HEEGAARD, P.M.H.; BISGAARD, M.; JENSEN, H.E. Pathology, Tissue Metalloproteinase Transcription and Haptoglobin Responses in Mice after Experimental Challenge with Different Isolates of Pasteurella multocida Obtained from Cases of Porcine Pneumonia. Journal of Comparative Pathology, v.145, n.2/3, p.251-260, 2011b. doi: 10.1016/j.jcpa.2011.01.002.

[23] RUDKIN, G.T.; STOLAR, B.D. High resolution detection of DNA-RNA hybrids in situ by indirect immunofluorescence. Nature, v.265, n.3, 1977.

[24] SUI, W.; OU, M.; CHEN, J.; WAN, Y.; PENG, H.; QI, M.; HUANG, H.; DAI, Y. Comparison of immuno histochemistry and fluorescence in situ hybridization assessment for Her-2 status in breast cancer. World Journal of Surgical Oncology, v.7, p.83, 2009.

[25] SWINGER R.R.; TUCKER J.D. Fluorescence in situ hybridization: a brief review. Environmental and Molecular Mutagenesis, v.27, p.245-254, 1996.

[26] TANKE, H.J.; DIRKS, R.W.; RAAP, T. FISH and immunocytochemistry: towards visualising single target molecules in living cells. Current Opinion in Biotechnology, v.16, p.49-54, 2005. doi: 10.1016/j.copbio.2004.12.001.

[27] VOLPI, E.V.; BRIDGER, J.M. FISH glossary: an overview of the fluorescence in situ hybridization technique. BioTechniques, v.45, p.385-409, 2008. doi: 10.2144/000112811.

[28] WOLCOT, M.J. Advances in Nucleic Acid-Based Detection Methods. Clinical Microbiology Reviews, v.5, n.4, p.370-386, 1992.

Brasil

Brasil

Hibridização in situ fluorescente: princípios básicos e perspectivas para o diagnóstico de doenças infecciosas em medicina veterinária

Fluorescent in situ hybridization: basic principles and perspectives for diagnosing infectious diseases in veterinary medicine

Resumos

Datas de Publicação

Histórico