Abstracts
The aim of this study was to determine if Toxoplasma gondii was present in aborted bovine fetuses in Brazil. Histopathology of 105 cases with suspected infectious abortion, analyzed during the period from 2006 to 2008 at Centro de Pesquisa e Desenvolvimento de Sanidade Animal of Instituto Biológico, São Paulo, showed 75 cases with indications of abortion due to apicomplexan protozoa. These cases were submitted to PCR for verification of the laboratory diagnosis. Fetal DNA was extracted from central nervous system, heart, liver, muscle, and/or placenta samples to obtain a 529 bp DNA fragment. T. gondii DNA was not detected in any of the bovine fetuses analyzed, suggesting that it may not be a frequent cause of bovine abortion.
Toxoplasma gondii; abortion; cattle; Brazil; Apicomplexa
O objetivo do presente trabalho foi avaliar a presença de Toxoplasma gondii em fetos bovinos abortados no Brasil. Com base em laudos histopatológicos, de um total de 105 casos com suspeita de aborto infeccioso, recebidos no período de 2006 a 2008, no Centro de Pesquisa e Desenvolvimento de Sanidade Animal do Instituto Biológico, São Paulo, 75 casos foram sugestivos de abortamento por protozoário Apicomplexa e foram submetidos à técnica de PCR para confirmação do diagnóstico laboratorial. O DNA foi extraído a partir de amostras de sistema nervoso central, coração, músculo, fígado e/ou placenta dos fetos para obtenção de um fragmento de DNA de 529pb. Não foi detectada a presença de DNA de T. gondii em qualquer dos fetos bovinos analisados, não sendo este agente infeccioso uma causa frequente de abortamentos.
Toxoplasma gondii; aborto; bovino; Brasil; Apicomplexa
- CABRAL, A.D.; CAMARGO, C.N.; GALLETI, N.T.C.; OKUDA, L.H.; PITUCO, E.M.; DEL FAVA, C. Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR. Revista Brasileira de Parasitologia Veterinária, v.18, n.4, p.14-19, 2009.
- CANADA, N.; MEIRELES, C.S.; ROCHA, A.; CORREIA DA COSTA, J.M.; ERICKSON, M.W.; DUBEY, J.P. Isolation of viable Toxoplasma gondii from naturally infected aborted bovine fetuses. Journal of Parasitology, v.88, n.6, p.1247-1248, 2002.
- DUBEY, J.P. A review of toxoplasmosis in cattle. Veterinary Parasitology, v.22, n.3/4, p.177-202, 1986.
- DUBEY, J.P. Review of Neospora caninum and neosporosis in animals. The Korean Journal of Parasitology, v.41, n.1, p.1-16, 2003.
- ELLIS, J.T. Polymerase chain reaction approaches for the detection of Neospora caninum and Toxoplasma gondii International Journal for Parasitology, v.28, n.7, p.1053-1060, 1998.
- GARCIA, J.L.; NAVARRO, I.T.; OGAWA, L.; OLIVEIRA , R.C. Soroprevalence of Toxoplasma gondii in swine, bovine, ovine and equine, and their correlation with human, felines and canines, from farms in North region of Paraná State, Brazil. Ciência Rural, v.29, n.1, p. 91-97, 1999.
- GOTTSTEIN, B.; HENTRICH, B.; WYSS, R.; THUR. B.; BUSATO, A.; STARK, K. D.; MULLER, N. Molecular and immunodiagnostic investigations on bovine neosporosis in Switzerland. International Journal of Parasitology, v. 28, n.4, p.679-691, 1998.
- HOMAN, W.L.; VERCAMMEN, M.; DE BRAEKELEER , J. Identification of a 200 to 300 fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. International Journal of Parasitology, v.30, n.1, p.69-75, 2000.
- HUGHES, M.J.; WILLIAMS, H.R.; MORLEY, K.E.; COOK, N.A.D.; TERRY, S.R.; MURPH, G.R.; SMITH, E.J.; HIDE, G. The prevalence of Neospora caninum and co-infection with Toxoplasma gondii by PCR analysis in naturally occurring mammal populations. Parasitology, v.132, n.1, p.29-36, 2006.
- MACEDO, M.F.S.B. de; MACEDO, C.A.B. de; EWALD, M.P.C.; MARTINS, G.F.; ZULPO, D.L.; CUNHA, I.A.L. da; TARODA, A.; CARDIM, S.T.; SU, C.; GARCIA, J.L. Isolation and genotyping of Toxoplasma gondii from pregnant dairy cows (Bos taurus) slaughtered. Revista Brasileira de Parasitologia Veterinária, v.21, n.1, p.74-77, 2012.
- MOORE, D.P.; REGIDOR-CERRILLO, J.; MORRELL, E.; POSO, D.B.; LEUNDA, M.R.; LINSCHINKY, L.; ODEÓN, A.C.; ODRIOZOLA, L.M.; ORTEGA-MORA, L.M.; CAMPERO, C.M. The role of Neospora caninum and Toxoplasma gondii in spontaneous bovine abortion in Argentina. Veterinary Parasitology, v.156, n.3/4, p.163-167, 2008.
- REITT, K.; HILBE, M.; VOEGTLIN, A.; CORBOZ, L.; HAESSIG, M.; POSPISCHIL, A. Aetiology of bovine abortion in Switzerland from 1986 to 1995 - a retrospective study with emphasis on detection of Neospora caninum and Toxoplasma gondii by PCR. Journal of Veterinary Medicine, v.54, n.1, p.15-22, 2007.
- SAGER, H.; FISCHER, I.; FURRER, K.; STRASSER, M.; WALDVOGEL, A. Neospora caninum-associated bovine abortions by PCR, histopathology and serology. Veterinary Parasitology, v.102, n.1/2, p.1-15, 2001.
- SANTOS, S.L.; SOUZA COSTA, H. de; GONDIM, L.Q.; SILVA, M.S. da; UZÊDA, R.S.; ABE-SANDES, K.; GONDIM, L.F. Investigation of Neospora caninum, Hammondia sp., and Toxoplasma gondii in tissues from slaughtered beef cattle in Bahia, Brazil. Parasitology Research, v.106, n.2, p.457-461, 2010.
- TENTER, A.M.; HECKEROTH, A.R.; WEISS, L.M. Toxoplasma gondii: from animals to humans. International Journal of Parasitology, v.30, n.12/13, p.1217-1258, 2000.
- YAMAGE, M.; FLECHTNER, O.; GOTTSTEIN, B. Neospora caninum: specific oligonucleotide primers for the detection of brain "cyst" DNA of experimentally infected nude mice by the polymerase chain reaction (PCR). Journal of Parasitology, v.82, n.2, p.272-279, 1996.
Publication Dates
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Publication in this collection
04 June 2013 -
Date of issue
Mar 2013
History
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Received
25 July 2011 -
Accepted
04 Nov 2012
