SciELO - Scientific Electronic Library Online

 
vol.80 número2Análise da micobiota associada à entomofauna em rações a granel para animais domésticosAlternativas para o controle de nematoides gastrintestinais de pequenos ruminantes índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

Compartilhar


Arquivos do Instituto Biológico

versão On-line ISSN 1808-1657

Arq. Inst. Biol. vol.80 no.2 São Paulo abr./jun. 2013

http://dx.doi.org/10.1590/S1808-16572013000200017 

COMUNICAÇÃO CIENTÍFICA SCIENTIFIC COMMUNICATION

 

First report of austrodiplostomum compactum and ithyoclinostomum dimorphum in trahira (hoplias malabaricus) from the middle course of the Rio Doce, Minas Gerais, Brazil

 

Primeiro relato de austrodiplostomum compactum e ithyoclinostomum dimorphum em traíras (hoplias malabaricus) provenientes do médio curso do Rio Doce, Minas Gerais, Brasil

 

 

F. BeleiI; S.R. FerreiraII; L.M. PerinII; F.R. BragaII; W.M.S. SampaioI; J.V. de AraújoII; J.A. DergamI; R.M. TakemotoIII

IUniversidade Federal de Viçosa, Departamento de Biologia Animal, Av. Ph Rolfs, s/no, CEP 36570-000, MG, Brasil. E-mail: ferreirasr2008@hotmail.com
II
Universidade Federal de Viçosa, Departamento de Medicina Veterinária, Viçosa, MG, Brasil
III
Universidade Estadual de Maringá, Núcleo de Pesquisas em Liminologia, Ictiologia e Aquicultura, Maringá, PR, Brasil

 

 


ABSTRACT

Austrodiplostomum compactum and Ithyoclinostomum dimorphum are two trematodes commonly found in trahira, but these parasites were never reported in trahiras from Rio Doce. Thus, the aim of this study is to describe the occurrence of A. compactum and I. dimorphum metacercariae in trahira from the middle course of the Rio Doce and to record the presence of eggs in I. dimorphum metacercariae. The parasites were identified and analyzed using methods described previously. There were found 10 A. compactum metacercariae in the aqueous humor of eyes in four of the trahiras and 12 I. dimorphum  metacercariae encysted in the peritoneal cavity in five of the trahiras. Maceration of the I. dimorphum metacercariae revealed the presence of eggs. These results demonstrate the broad distribution of these parasites and the first report of these parasites in trahira from Rio Doce.

Key words: Metacercariae, Austrodiplostomum compactum, Ithyoclinostomum dimorphum, Rio Doce.


RESUMO

Austrodiplostomum compactum e Ithyoclinostomum dimorphum são dois trematódeos comumente encontrados em traíras, contudo, esses parasitas nunca foram relatados em traíras do Rio Doce. O objetivo do estudo é descrever a ocorrência de metacercárias de A. compactum e I. dimorphum, e presença de ovos em metacercárias de I. dimorphum em traíras provenientes do médio curso do Rio Doce. Os parasitas foram analisados e identificados utilizando métodos descritos anteriormente. Foram encontradas 10 metacercárias de A. compactum no humor aquoso dos olhos em quatro traíras e 12 metacercárias de I. dimorphum encistadas na cavidade peritoneal de cinco traíras. A maceração de metacercárias de I. dimorphum revelou a presença de ovos nestes parasitos. Esses resultados demonstram a ampla distribuição desses trematódeos e este é o primeiro relato destes parasitos em traíra do Rio Doce.

Palavras-chave: Metacercárias, Austrodiplostomum compactum, Ithyoclinostomum dimorphum, Rio Doce.


 

 

Our current knowledge of Brazilian ichthyoparasites indicates that there are approximately 1050 species from 620 different host species (Eiras et al., 2010). This number may be underestimated, since most studies on ichthyoparasitology are concentrated in southern Brazil (Pavanelli et al., 1990; Gallio  et al., 2007; Yamada et al., 2008). In southeast Brazil the ichthyo-parasite fauna has not been well explored (Martins et al., 1999; Brasil-Sato, 2002; Paraguassú; Luque, 2007). Thus, the data obtained to date are not sufficient to elucidate the parasite fauna of Neotropical fishes.

The trahira, Hoplias malabaricus Bloch, 1794, has a wide distribution in Central and South America. This fish has nocturnal habits, tolerate low dissolved oxygen concentrations and its abundance is correlated with aquatic vegetation and shallow waters (Shibatta et al., 2002; Reis et al., 2003). According to Luque; Poulin (2007), there are currently 67 species of parasites recorded in H. malabaricus. Due to their feeding habits these species may act as definitive, intermediate and paratenic hosts to helminths (Barros et al., 2007). Austrodiplostomum compactum (Lutz, 1928) and Ithyoclinostomum dimorphum (Diesing, 1850) are trematodes commonly found in trahira. A. compactum is a digenetic trematode belonging to the order Diplostomida, whose metacercaria parasitise the eyes, brain, pericardium, muscle and peritoneal cavity of many Neotropical fishes (Eiras et al., 2010). A. compactum metacercariae have dorsoventrally flattened bodies, small subterminal oral suckers and two pseudosuckers in the anterior region, an acetabulum and short esophagus (Kohn et al., 1995). I. dimorphum is also a diplostomidae digenetic trematode whose metacercariae are found encysted in the muscle, gills, pericardium, peritoneal cavity and oesophagus of fish bodies and have very elongate, narrow, flat bodies with varying length and width (Pavanelli et al., 1990).

The aim of this study was to describe for the first time the occurrence of A. compactum and I. dimorphum metacercariae in trahiras from the middle course of the Doce River, and to record the occurrence of eggs in I. dimorphum metacercariae.

The seven specimens of H. malabaricus Bloch, 1794, were collected in Lago Carioca, a complex of lagoons in the Parque Estadual do Rio Doce (PERD) (19S 46' 53" and 42W 35' 57"). The specimens were collected using gillnets, standardized in various mesh sizes, mounted in the evening and removed the following morning.

The fishes were sedated with clove oil, euthanized and then fixed in formalin at 10% concentration and stored at the Museu de Zoologia "João Moojen" – Universidade Federal de Viçosa (MZUFV) until the analysis.

The specimens eyes were removed and the abdominal cavity was opened to search for and collect the internal parasites. The parasites were identified and analyzed using methods described by Pavanelli et al. (1990), Kohn et al. (1995) and Eiras et al. (2010). Moreover, the parasites were subjected to maceration and observed under a stereomicroscope.

There were found a total of 10 A. compactum,  metacercariae (Fig. 1) in the aqueous humor of eyes in four of the trahiras and 12 I. dimorphum, metacercariae (Fig. 2) encysted in the peritoneal cavity in five of the trahiras. The occurrence of these parasites were previously reported in other fish species in different geographic regions (Pavanelli et al., 1990; Kohn et al., 1995; Machado et al., 1996; Silva - Souza, 1998; Gallio et al., 2007; Paraguassú; Luque, 2007; Yamada et al., 2008; Zica et al., 2009; Paes et al., 2010), but this is the first report of the presence of these parasites in trahira from middle course of the Rio Doce.

 

 

 

Based on analysis by stereomicroscope, the metacercariae of A. compactum presented a dorsoventrally flattened body, subterminal oral sucker, two pseudosuckers in the anterior region, an acetabulum and a short esophagus. The average length of the specimens was 1,121 μm (645-1,227 μm) and the average width measured in the midline of the body was 656 μm (312-987 μm). These morphological characteristics are consistent with other studies (Kohn et al., 1995).

The metacercariae is capable to penetrate in different structures such as body surface, fins, gills and mouth cavity, and the larvae migrate to the eye, and can cause exophthalmoses, retinal detachment, cataract, blindness, lesions and haemorrhages in various tissues, as well as obstruction of blood vessels (Szidat; Nani, 1951). Also these phenomena can lead to fish deaths in cases of high rates of infection (Overstreet; Curran, 2004).

I. dimorphum presented elongate, narrow, flat body. Morphological characteristics in according to Pavanelli et al. (1990). It was not possible to compress the metacercariae between slides because their body was hardened due to their fixation in formalin. These digeneans occur preferentially in the south of Brazil (Gallio et al., 2007), but their distribution extends into southeast Brazil, namely Rio Doce, as described in the present study.

According to Dias et al. (2003) and Pinto et al. (2004), I. dimorphum adults are found in the bird Ardea cocoi, a species found in the upper Parana region, and in birds belonging to the order Ciconiiformes from central-west Brazil. These birds distributed from Panama to Chile, Argentina and Brazil (Sick, 1997). A. cocoi has been reported in Parque do Rio Doce (PERD). This species occurs occasionally and is migratory (Machado; Fonseca, 2000; Lins, 2001; Faria et al., 2006) and may be acting as a disseminator of the parasite. However, there are no reports in the literature regarding the occurrence of I. dimorphum in birds from this region.

The macerate of the I. dimorphum metacercariae revealed the presence of eggs (Fig. 3). The occurrence of eggs in trematode metacercariae is not uncommon, for example, Coitocaecum parvum (Poulin; Cribb, 2002) and Microphallus papillorobustus (Wang; Thomas, 2002). The temporary or permanent absence of a definitive host may be responsible for the shortening of the parasite life cycle (Poulin; Cribb, 2002). I. dimorphum has a complex life cycle, where molluscs, fish and birds act as hosts at different life stages of the parasite (Dias et al., 2003). However, the role of each host is not yet fully understood. Thus, this first report on the occurrence of eggs in I. dimorphum metarcercariae from fish may help to explain the cycle of trematodes.

 

 

This is the first report of the occurrence of A. compactum and I. dimorphum metacercariae in trahiras from the middle course of the Rio Doce. Fish infection by these parasites can cause major economic losses, related to pisciculture and fishing activities, due to the reduction of fish stock and products of lower quality. This report is also important as it contributes to the knowledge of parasite distribution in Brazil.

 

REFERENCES

BARROS, L.A.; MORAES FILHO, J.; OLIVEIRA, R.L. Larvas de nematóides de importância zoonótica encontradas em traíras (Hoplias malabaricus Bloch, 1794) no município de Santo Antonio do Leverger, MT. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v.59, p.533-535, 2007.         [ Links ]

BRASIL-SATO, M.C. Digenea of Salminus brasiliensis (Cuvier, 1817) (Osteichthyes, haracidae) of the São Francisco river basin, Brazil. Revista Brasileira de Parasitologia Veterinária, v.11, p. 95-98, 2002.         [ Links ]

DIAS, M.L.G.G.; SANTOS, M.J.; SOUZA, G.T.R.; MACHADO, M.H.; PAVANELLI, G.C. Scanning electron microscopy of Ithyoclinostomum dimorphum (Trematoda: Clinostomidae), a parasite of Ardea cocoi (Aves: Ardeidea). Parasitology Research, v.90, p.355-358, 2003.         [ Links ]

EIRAS, J.C.; TAKEMOTO, R.M.; PAVANELLI, G.C. Diversidade dos parasitas de peixes de água doce do Brasil. Maringá: Clichetec, 2010. 333p.         [ Links ]

FARIA, C.M.A.; RODRIGUES, M.; AMARAL, F.Q., MÓDENA, E.; FERNANDES, A.M. Aves em fragmento de Mata Atlântica no alto Rio Doce, Minas Gerais: colonização e extinção. Revista Brasileira de Zoologia, v.23, p.1217-1230, 2006.         [ Links ]

GALLIO, M.; SILVA, A.S.; SOARES, J.F.; SILVA, M.K.; SALOMÃO, E.L.; MONTEIRO, S.G. Ocorrência de metacercárias de ithyoclinostomum dimorphum em traíras no Rio Grande do Sul, Brasil: relato de caso. Estudos de Biologia, v.29, p.337-339, 2007.         [ Links ]

KOHN, A.; FERNANDES, B.M.M.; BAPTISTA-FARIAS, M.F.D. Metacercariae of Diplostomum (Austrodiplostomum) compactum (Tematoda, Diplostomatidae) in the eyes of Plagioscion squamosissimus (Teleostei, Sciaenidae) from the Reservoir of the Hidroelectric Power Station of Itaipu, Brazil. Memórias do Instituto Oswaldo Cruz, v.90, p.341-344, 1995.         [ Links ]

LINS, L.V. Diagnóstico ornitológico do Parque Estadual do Rio Doce: Relatório técnico. Belo Horizonte, 2001. Disponível em: <http://www.ief.mg.gov.br>. Acesso em: 1 dez. 2011.         [ Links ]

LUQUE, J.L.; POULIN, R. Metazoan parasite species richness in Neotropical fishes: Hotspots and the geography of biodiversity. Parasitology, v.134, p.865-878, 2007.         [ Links ]

MACHADO, R.B.; FONSECA, G.A.B.F. The avifauna of rio Doce valley, southeastern Brazil, a highly fragmented area. Biotropica, v.32, p.914-924. 2000.         [ Links ]

MACHADO, M.H.; PAVANELLI, G.C.; TAKEMOTO, R.M. Structure and diversity of endoparasitic infracommunities and the trophic level of Pseudoplatystoma corruscans and Schizodon Borelli (Osteichthyes) of the high Paraná river. Memórias do Instituto Oswaldo Cruz, v.91, p.441-448, 1996.         [ Links ]

MARTINS, M.L.; FUJIMOTO, R.Y.; NASCIMENTO, A.A.; MORAES, F.R. Ocorrência de Diplostomum sp. Nordmann, 1832 (Digenea: Diplostomidae) em Plagioscion squamosissimus (Heckel, 1840) proveniente do reservatório de Volta Grande, MG, Brasil. Acta Scientiarum, v.21, p.263-266, 1999.         [ Links ]

OVERSTREET, R.M.; CURRAN, S.S. Defeating diplostomoid dangers in USA catfish aquaculture. Folia Parasitologica, v.51, p.153-165, 2004.         [ Links ]

PAES, J.V.K.; CARVALHO, E.D.; SILVA, R.J. Infection by Austrodiplostomum compactum metacercariae in fish from the Nova Avanhandava reservoir, Tietê river, São Paulo State, Brazil. Acta Scientiarum. Biological Sciences, v.32, p.273-278, 2010.         [ Links ]

PARAGUASSÚ, A.R.; LUQUE, J.L. Metazoários parasitos de seis espécies de peixes do reservatório de Lajes, estado do Rio de Janeiro, Brasil. Revista Brasileira de Parasitologia Veterinária, v.16, p.121-128, 2007.         [ Links ]

PAVANELLI, G.C.; SCHAEFFER, G.V.; SANTOS, M.S. Ocorrência e histopatologia de Ithyoclinostomum dimorphum (Diesing, 1850) (Trematoda: Clinostomatidae) em traíras coletadas no rio Paraná. Revista Unimar, v.12, p.69-75, 1990.         [ Links ]

PINTO, R.M.; BARROS, L.A.; TORTELLY, L.; TEI-XEIRA, R.F.; GOMES, D.C. Prevalence and pathology of helminths of ciconiiform birds from the Brazilian swamplands. Journal of Helmintology, v.78, p.259-264, 2004.         [ Links ]

POULIN, R.; CRIBB, T.H. Trematode life cycles: short is sweet? Trends in Parasitology, v.18, p.176-183, 2002.         [ Links ]

REIS, R.E.; KULLANDER, S.O.; FERRARIS, J.C.J. Check list of the freshwater fishes of South and Central America. Porto Alegre: EDIPUCRS, 2003.729p.         [ Links ]

SHIBATTA, O.A.; ORSI, M.L.; BENNEMANN, S.T.; SILVA E SOUZA, A.T. Diversidade e distribuição de peixes na bacia do rio Tibagi. In: MEDRI, M.E.; BIANCHINI, E.; SHIBATTA, O.A.; PIMENTA, J.A. (Ed.). A Bacia do Rio Tibagi. Londrina: EDUEL, 2002. p.403-423.         [ Links ]

SICK, H. Ornitologia brasileira. Rio de Janeiro: Nova Fronteira, 1997. 912p.         [ Links ]

SILVA-SOUZA, A. T. Estudo do parasitismo de Plagioscion squamosissmus (Heckel, 1940) (Perciformes, Sciaenidae) por Diplostomum (Austrodiplostomum) compactum (Lutz, 1928) (Trematoda, Digenea) no Rio Tibagi, PR. 1998. 125f. Tese (Doutorado em Ciências) - Universidade Federal de São Carlos, São Carlos, SP, 1998.         [ Links ]

SZIDAT, L.; NANI, A.  Diplostostomiasis cerebralis delpejerrey. Una grave epizootia que afecta la economia nacional producida por larvas de trematodes que destruyen el cerebro de los pejerreys. Revista del Instituto Nacional de Investigacion de las Ciencias Naturales, v.1, n.8, p.323-384; 1951.         [ Links ]

WANG, C.L.; THOMAS, F. Egg production by metacercariae of Microphallus papillorobustus: a reproductive insurance? Journal of Helminthology, v.76, p.279-28, 2002.         [ Links ]

YAMADA, F.H.; MOREIRA, L.H.A.; CESCHINI, T.L.; TAKEMOTO, R.M.; PAVANELLI, G.C. Novas ocorrências de metacercária de Austrodiplostomum compactum (Lutz, 1928) (Platyhelminthes: digenea) parasito de olhos de peixes da bacia do rio Paraná. Revista Brasileira de Parasitologia Veterinária, v.17, p.163-168, 2008.         [ Links ]

ZICA, E.O.P.; SANTOS, K.R.; RAMOS, I.P.; ZANATTA, A.S.; CARVALHO, E.D.; SILVA, R.J. First case of an infection of the metacercariae of Austrodiplostomum compactum (Lutz, 1928) (Digenea, Diplostomidae) in Hypostomus regani (Ihering, 1905) Siluriformes: Loricariidae). Pan-American Journal of Aquatic Sciences, v.4, p.35-38, 2009.         [ Links ]

 

 

Received on 17/12/11
Accepted on 9/4/13

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons