Produção in vitro de embriões de bovinos da raça Nelore oriundos de ovócitos de ovários com e sem corpo lúteo

Barbosa, Cristiano Pereira; Toniollo, Gilson Hélio; Guimarães, Ednaldo Carvalho

doi:10.5216/cab.v14i1.12588

Resumos

Comparou-se a produção de embriões a partir de ovócitos oriundos de ovários com e sem corpo lúteo de vacas da raça Nelore, em relação à qualidade e quantidade de ovócitos obtidos, embriões produzidos, taxa de prenhez e proporção dos sexos. Foram realizadas aspirações foliculares dos dois ovários de 219 vacas em 250 seções, igualmente distribuídas em cinco grupos, sendo G1=fêmeas gestantes; G2=não gestantes, com CL e submetidas a tratamento hormonal com progestágeno+BE+PGF2∝; G3=não gestantes, sem CL e com o mesmo tratamento de G2; G4=não gestantes, com presença de CL e sem tratamento; G5=não gestantes, sem presença de CL e sem tratamento. Os resultados foram avaliados pela análise de variância (ANOVA) e aplicado o Teste de Tukey para comparação entre médias, a 5% de significância, pelo programa SISVAR. Já para comparação de proporção de sexos dentro e entre os grupos, foi utilizado o teste de comparação múltipla de proporções. O G4 produziu maior média de ovócitos totais aspirados que G2 e G3. Comparando-se os três grupos que apresentavam CL (G1, G2, G4), o G4 foi superior a G1 e G2 em ovócitos totais, no ovário com e sem CL. Em ovócitos viáveis, G2 foi superior a G1 no ovário com CL. Os ovócitos obtidos de ovários com CL é que resultaram em melhores índices de produção de embriões. As vacas gestantes produziram melhor nos ovários sem CL, com mais ovócitos viáveis (p<0,05) e iguais em embriões (p>0,05), em relação ao ovário com CL. As taxas de prenhez e proporção dos sexos foram semelhantes entre os grupos (p>0,05). A concentração de progesterona (P4) foi diferente entre os grupos, mas não influenciou as variáveis analisadas. A utilização do tratamento hormonal não melhorou os resultados de nenhuma variável. Portanto, o melhor critério de escolha de doadoras Nelore para programas de Produção in vitro (PIV) de embriões é o conhecimento individual de cada animal ao longo das seções de aspiração folicular.

aspiração folicular; progesterona sérica; reprodução animal

This study aimed to compare the quality and quantity of oocytes aspirated from ovaries containing or not corpus luteum (CL), the development of embryos, pregnancy rate and sex ratio among embryos after IVP in Nellore cows. Follicular aspirations were performed in both ovaries of 219 cows in 250 sections, and equally divided into five groups: G1 = pregnant, G2 = not pregnant, with CL and submitted to hormonal therapy with progestin+BE+PGF2∝; G3 = not pregnant, without CL and with the same treatment of G2; G4 = not pregnant, with the presence of CL and no treatment; G5 = not pregnant, without the presence of CL and no treatment. The statistical analysis was performed with analysis of variance (ANOVA) and Tukey Test was applied to compare means at 5% significance level, by SISVAR program. For the comparison of sex ratio within and between groups, the multiple comparison test of proportions was used. The G4 produced higher mean of total aspirated oocytes than G2 and G3. Comparing the three groups with CL, G4 showed higher number of oocytes than G1 and G2 in both ovaries. The G2 presented more viable oocytes than G1 in the CL-containing ovary. The ovaries without CL showed better production in pregnant cows despite of viable oocytes (p<0.05), and similar embryo production (p>0.05) when compared with ovaries presenting CL. Oocytes obtained from ovaries with CL resulted in better rates of embryo production. Pregnancy rates and sex ratio were similar among groups (p>0.05). The P4 concentration was different between groups, but it did not influence any variable. The use of hormonal treatment did not improve the results of any variable. Therefore, choosing Nellore donors for IVP programs depends on knowing each animal individually along the sections of OPU.

animal reproduction; follicular aspiration; serum progesterone

AVERY, B.; MADISON, V.; GREVE, T. Sex and development in bovine in vitro embryo production. Theriogenology, v. 35, n. 5, p. 953-963, 1991.
BACELAR, D.; MAX, M.C.; PADILHA, L.C.; BARREIROS, T.R.R.; SENEDA, M.M. Incremento na obtenção de oócitos em novilhas Nelore (Bos taurus indicus) tratadas com progesterona injetável e benzoato de estradiol. Semina: Ciências Agrárias, Londrina, v. 31, n. 1, p. 163-172, 2010.
BAVISTER, B.D.; HELLEKANT, T.A.R.; PINYOPUMMINTR, T. Development of in vitro matured/in vitro fertilized bovine embryos into morulae and blastocysts in defined culture media. Theriogenology, v. 37, n. 1, p. 127-146, 1992.
BIASE, N.G.; FERREIRA, D.F. Comparações múltiplas e testes simultâneos para parâmetros binomiais de K populações independentes. Revista Brasileira de Biometria, v. 27, n. 3, p. 301-323, 2009.
BLONDIN, P.; BOUSQUET, D.; TWAGIRAMUNGU, H.; BARNES, F.; SIRARD, M.A. Manipulation of follicular development to produce developmentally competent bovine oocytes. Biology of Reproduction, v. 66, n. 1, p. 38-43, 2002.
CAMARGO, L.S.; FREITAS, C.; de SÁ, W. F.; de MORAES FERREIRA, A.; SERAPIÃO R.V.; VIANA, J.H. Gestation length, birth weight and offspring gender ratio of in vitro-produced Gyr (Bos indicus) cattle embryos. Animal Reproduction Science, v. 120, n. 1-4, p. 10-15, 2010.
CHAUBAL, S.A.; FERRE, L.B.; MOLINA, J.A.; FABER, D.C.; BOLS, P.E.; REZAMAND, P.; TIAN, X.; YANG, X. Hormonal treatments for increasing the oocyte and embryo production in an OPU-IVP system. Theriogenology, v. 67, n. 4, p. 719-728, 2007.
CHIAN, R.C.; CHUNG, J.T.; DOWNEY, B.R.; TAN, S.L. Maturational and developmental competence of immature oocytes retrieved from bovine ovaries at different phases of folliculogenesis. Reproductive Biomedicine Online, v. 4, n. 2, p. 127-132, 2002.
DE LOOS, F.; KASTRUP, P.; VAN MAURIK, P.; VAN BENEDEN, T.H.; KRUIP, T.A. Heterologous cell contacts and metabolic coupling in bovine cumulus oocyte complexes. Molecular Reproduction and Development, v. 28, n. 3, p. 255-259, 1991.
DOMÍNGUEZ, M.M. Effects of body condition, reproductive status and breed on follicular population and oocyte quality in cows. Theriogenology, v. 43, n. 8, p. 1405-1418, 1995.
GOODHAND, K.L.; STAINES, M.E.; HUTCHINSON, J.S.; BROADBENT, P.J. In vivo oocyte recovery and in vitro embryo production from bovine oocyte donors treated with progestagen, oestradiol and FSH. Animal Reproduction Science, v. 63, n. 3-4, p. 145–158, 2000.
GUTIÉRREZ-ADÁN, A.; LONERGAN, P.; RIZOS, D.; WARD, F.A.; BOLAND, M.P.; PINTADO, B.; FUENTE, J. Effect of the in vitro culture system on the kinetics of blastocyst development and sex ratio of bovine embryos. Theriogenology, v. 55, n. 5, p. 1117-1126, 2001.
HOLM, P.; BOOTH, P.J.; SCHMIDT, M.H.; GREVE, T.; CALLESEN, H. High bovine blastocyst development in a static in vitro production system using sofaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins. Theriogenology, v. 52, n. 4, p. 683-700, 1999.
MACHADO, G.M.; CARVALHO, J.O.; FILHO, E.S.; CAIXETA, E.S.; FRANCO, M.M.; RUMPF, R.; DODE, M.A. Effect of Percoll volume, duration and force of centrifugation, on in vitro production and sex ratio of bovine embryos. Theriogenology, v. 71, n. 8, p. 1289-1297, 2009.
MACHATKOVÁ, M.; JOKESOVÁ, E.; HORKY, F.; KREPELOVÁ, A. Utilization of the growth phase of the first follicular wave for bovine oocyte collection improves blastocyst production. Theriogenology, v. 54, n. 4, p. 543-550, 2000.
MACHATKOVÁ, M.; JOKESOVÁ, E.; PETELÍKOVÁ, J.; DVORÁCEK, V. Developmental competence of bovine embryos derived from oocytes collected at various stages of the estrous cycle. Theriogenology, v. 45, n. 4, p. 801-810, 1996.
MACHATKOVÁ, M.; KRAUSOVÁ, K.; JOKESOVÁ, E.; TOMANEK, M. Developmental competence of bovine oocytes: effects of follicle size and the phase of follicular wave on in vitro embryo production. Theriogenology, v. 61, n. 3, p. 329-335, 2004.
MANJUNATHA, B.M.; GUPTA, P.S.; RAVINDRA, J.P.; DEVARAJ, M.; RAMESH, H.S.; NANDI, S. In vitro developmental competence of buffalo oocytes collected at various stages of the estrous cycle. Theriogenology, v. 68, n. 6, p. 882-888, 2007.
MEINTJES, M.; BELLOW, M.S.; BROUSSARD, J.R.; PAUL, J.B.; GODKE, R.A. Transvaginal aspiration of oocytes from hormone-treated pregnant beef for in vitro fertilization. Journal of Animal Science, v. 73, n. 4, p. 967-974, 1995.
PARRISH, J.J.; SUSKO-PARRISH, J.L.; LEIBFRIED-RUTLEDGE, M.L.; CRITSER, E.S.; EYESTONE, W.H.; FIRST, N.L. Bovine in vitro fertilization with frozen-thawed semen. Theriogenology, v. 25, n. 4, p. 591-600, 1986.
PFEIFER, L.F.M.; SARTORI, R.; PIVATO, I.; RUMPF, R.; NOGUEIRA, G.P.; XAVIER, E.G.; DIONELLO, N.J.L.; CORRÊA, M.N. Effect of circulating progesterone on in vitro developmental competence of bovine oocytes. Animal Reproduction, v. 6, n. 3, p. 473-480, 2009.
RAMOS, A.F.; RUMPF, R.; CÂMARA, J.U.; MOLLO, M.R.; PIVATO, I.; MARQUES, A.P.Jr.; SARTORI, R. Effect of follicular wave synchronization on in vitro embryo production in heifers. Animal Reproduction Science, v. 117, n. 3-4, p. 201-207, 2010.
REIS, A.; METELO, R.; SANTOS, P.; SILVA, F. M. Efeito da estrutura ovárica e da idade de bovinos da raça Holstein Friesian na quantidade e qualidade de ovócitos e de embriões produzidos in vitro Brazilian Journal of Veterinary Research and Animal Science, v. 43, n. 5, p. 629-636, 2006.
REIS, A.; STAINES, M.E.; WATT, R.G.; DOLMAN, D.F.; McEVOY, T.G. Embryo production using defined oocyte maturation and zygote culture media following repeated ovum pick-up (OPU) from FSH-stimulated Simmental heifers. Animal Reproduction Science, v. 72, n. 3-4, p. 137-151, 2002.
SAUVÉ, R. Ultrasound guided follicular aspiration and in vitro fertilization. Arquivos da Faculdade de Veterinaria UFRGS, v. 26, n. 1, p. 141-155, 1998.
SCHAMS, D.; BERISHA, B. Regulation of Corpus Luteum Function in Cattle – an Overview. Reproduction in Domestic Animals, v. 39, n. 4, p. 241–251, 2004.
SUGULLE, A.H.; DOCHI, O.; KOYAMA, H. Developmental competence of bovine oocytes selected by Brilliant Cresyl Blue Staining: Effect of the presence of corpus luteum on embryo development. Journal of Mammalian Ova Research, v. 25, n. 1, p. 50-55, 2008.
TAKUMA, T.; OTSUBO, T.; KUROKAWA, Y.; OTOI, T. Effects of the corpus luteum within the ovary on the follicular dynamics after follicular aspiration and on the developmental competence of aspired oocytes. Reproduction, Fertility and Development, v. 19, n. 1, p. 324, 2007.
TAKUMA, T.; SAKAI, S.; EZOE, D.; ICHIMARU, H.; JINNOUCHI, T.; KAEDEI, Y.; NAGAI, T.; OTOI, T. Effects of season and reproductive phase on the quality, quantity and developmental competence of oocytes aspirated from Japanese black cows. The journal of reproduction and development, v. 56, n. 1, p. 55-59, 2010.

Datas de Publicação

Publicação nesta coleção
27 Set 2013
Data do Fascículo
Mar 2013

Histórico

Recebido
10 Dez 2010
Aceito
19 Dez 2012

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] AVERY, B.; MADISON, V.; GREVE, T. Sex and development in bovine in vitro embryo production. Theriogenology, v. 35, n. 5, p. 953-963, 1991.

[2] BACELAR, D.; MAX, M.C.; PADILHA, L.C.; BARREIROS, T.R.R.; SENEDA, M.M. Incremento na obtenção de oócitos em novilhas Nelore (Bos taurus indicus) tratadas com progesterona injetável e benzoato de estradiol. Semina: Ciências Agrárias, Londrina, v. 31, n. 1, p. 163-172, 2010.

[3] BAVISTER, B.D.; HELLEKANT, T.A.R.; PINYOPUMMINTR, T. Development of in vitro matured/in vitro fertilized bovine embryos into morulae and blastocysts in defined culture media. Theriogenology, v. 37, n. 1, p. 127-146, 1992.

[4] BIASE, N.G.; FERREIRA, D.F. Comparações múltiplas e testes simultâneos para parâmetros binomiais de K populações independentes. Revista Brasileira de Biometria, v. 27, n. 3, p. 301-323, 2009.

[5] BLONDIN, P.; BOUSQUET, D.; TWAGIRAMUNGU, H.; BARNES, F.; SIRARD, M.A. Manipulation of follicular development to produce developmentally competent bovine oocytes. Biology of Reproduction, v. 66, n. 1, p. 38-43, 2002.

[6] CAMARGO, L.S.; FREITAS, C.; de SÁ, W. F.; de MORAES FERREIRA, A.; SERAPIÃO R.V.; VIANA, J.H. Gestation length, birth weight and offspring gender ratio of in vitro-produced Gyr (Bos indicus) cattle embryos. Animal Reproduction Science, v. 120, n. 1-4, p. 10-15, 2010.

[7] CHAUBAL, S.A.; FERRE, L.B.; MOLINA, J.A.; FABER, D.C.; BOLS, P.E.; REZAMAND, P.; TIAN, X.; YANG, X. Hormonal treatments for increasing the oocyte and embryo production in an OPU-IVP system. Theriogenology, v. 67, n. 4, p. 719-728, 2007.

[8] CHIAN, R.C.; CHUNG, J.T.; DOWNEY, B.R.; TAN, S.L. Maturational and developmental competence of immature oocytes retrieved from bovine ovaries at different phases of folliculogenesis. Reproductive Biomedicine Online, v. 4, n. 2, p. 127-132, 2002.

[9] DE LOOS, F.; KASTRUP, P.; VAN MAURIK, P.; VAN BENEDEN, T.H.; KRUIP, T.A. Heterologous cell contacts and metabolic coupling in bovine cumulus oocyte complexes. Molecular Reproduction and Development, v. 28, n. 3, p. 255-259, 1991.

[10] DOMÍNGUEZ, M.M. Effects of body condition, reproductive status and breed on follicular population and oocyte quality in cows. Theriogenology, v. 43, n. 8, p. 1405-1418, 1995.

[11] GOODHAND, K.L.; STAINES, M.E.; HUTCHINSON, J.S.; BROADBENT, P.J. In vivo oocyte recovery and in vitro embryo production from bovine oocyte donors treated with progestagen, oestradiol and FSH. Animal Reproduction Science, v. 63, n. 3-4, p. 145–158, 2000.

[12] GUTIÉRREZ-ADÁN, A.; LONERGAN, P.; RIZOS, D.; WARD, F.A.; BOLAND, M.P.; PINTADO, B.; FUENTE, J. Effect of the in vitro culture system on the kinetics of blastocyst development and sex ratio of bovine embryos. Theriogenology, v. 55, n. 5, p. 1117-1126, 2001.

[13] HOLM, P.; BOOTH, P.J.; SCHMIDT, M.H.; GREVE, T.; CALLESEN, H. High bovine blastocyst development in a static in vitro production system using sofaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins. Theriogenology, v. 52, n. 4, p. 683-700, 1999.

[14] MACHADO, G.M.; CARVALHO, J.O.; FILHO, E.S.; CAIXETA, E.S.; FRANCO, M.M.; RUMPF, R.; DODE, M.A. Effect of Percoll volume, duration and force of centrifugation, on in vitro production and sex ratio of bovine embryos. Theriogenology, v. 71, n. 8, p. 1289-1297, 2009.

[15] MACHATKOVÁ, M.; JOKESOVÁ, E.; HORKY, F.; KREPELOVÁ, A. Utilization of the growth phase of the first follicular wave for bovine oocyte collection improves blastocyst production. Theriogenology, v. 54, n. 4, p. 543-550, 2000.

[16] MACHATKOVÁ, M.; JOKESOVÁ, E.; PETELÍKOVÁ, J.; DVORÁCEK, V. Developmental competence of bovine embryos derived from oocytes collected at various stages of the estrous cycle. Theriogenology, v. 45, n. 4, p. 801-810, 1996.

[17] MACHATKOVÁ, M.; KRAUSOVÁ, K.; JOKESOVÁ, E.; TOMANEK, M. Developmental competence of bovine oocytes: effects of follicle size and the phase of follicular wave on in vitro embryo production. Theriogenology, v. 61, n. 3, p. 329-335, 2004.

[18] MANJUNATHA, B.M.; GUPTA, P.S.; RAVINDRA, J.P.; DEVARAJ, M.; RAMESH, H.S.; NANDI, S. In vitro developmental competence of buffalo oocytes collected at various stages of the estrous cycle. Theriogenology, v. 68, n. 6, p. 882-888, 2007.

[19] MEINTJES, M.; BELLOW, M.S.; BROUSSARD, J.R.; PAUL, J.B.; GODKE, R.A. Transvaginal aspiration of oocytes from hormone-treated pregnant beef for in vitro fertilization. Journal of Animal Science, v. 73, n. 4, p. 967-974, 1995.

[20] PARRISH, J.J.; SUSKO-PARRISH, J.L.; LEIBFRIED-RUTLEDGE, M.L.; CRITSER, E.S.; EYESTONE, W.H.; FIRST, N.L. Bovine in vitro fertilization with frozen-thawed semen. Theriogenology, v. 25, n. 4, p. 591-600, 1986.

[21] PFEIFER, L.F.M.; SARTORI, R.; PIVATO, I.; RUMPF, R.; NOGUEIRA, G.P.; XAVIER, E.G.; DIONELLO, N.J.L.; CORRÊA, M.N. Effect of circulating progesterone on in vitro developmental competence of bovine oocytes. Animal Reproduction, v. 6, n. 3, p. 473-480, 2009.

[22] RAMOS, A.F.; RUMPF, R.; CÂMARA, J.U.; MOLLO, M.R.; PIVATO, I.; MARQUES, A.P.Jr.; SARTORI, R. Effect of follicular wave synchronization on in vitro embryo production in heifers. Animal Reproduction Science, v. 117, n. 3-4, p. 201-207, 2010.

[23] REIS, A.; METELO, R.; SANTOS, P.; SILVA, F. M. Efeito da estrutura ovárica e da idade de bovinos da raça Holstein Friesian na quantidade e qualidade de ovócitos e de embriões produzidos in vitro Brazilian Journal of Veterinary Research and Animal Science, v. 43, n. 5, p. 629-636, 2006.

[24] REIS, A.; STAINES, M.E.; WATT, R.G.; DOLMAN, D.F.; McEVOY, T.G. Embryo production using defined oocyte maturation and zygote culture media following repeated ovum pick-up (OPU) from FSH-stimulated Simmental heifers. Animal Reproduction Science, v. 72, n. 3-4, p. 137-151, 2002.

[25] SAUVÉ, R. Ultrasound guided follicular aspiration and in vitro fertilization. Arquivos da Faculdade de Veterinaria UFRGS, v. 26, n. 1, p. 141-155, 1998.

[26] SCHAMS, D.; BERISHA, B. Regulation of Corpus Luteum Function in Cattle – an Overview. Reproduction in Domestic Animals, v. 39, n. 4, p. 241–251, 2004.

[27] SUGULLE, A.H.; DOCHI, O.; KOYAMA, H. Developmental competence of bovine oocytes selected by Brilliant Cresyl Blue Staining: Effect of the presence of corpus luteum on embryo development. Journal of Mammalian Ova Research, v. 25, n. 1, p. 50-55, 2008.

[28] TAKUMA, T.; OTSUBO, T.; KUROKAWA, Y.; OTOI, T. Effects of the corpus luteum within the ovary on the follicular dynamics after follicular aspiration and on the developmental competence of aspired oocytes. Reproduction, Fertility and Development, v. 19, n. 1, p. 324, 2007.

[29] TAKUMA, T.; SAKAI, S.; EZOE, D.; ICHIMARU, H.; JINNOUCHI, T.; KAEDEI, Y.; NAGAI, T.; OTOI, T. Effects of season and reproductive phase on the quality, quantity and developmental competence of oocytes aspirated from Japanese black cows. The journal of reproduction and development, v. 56, n. 1, p. 55-59, 2010.

Brasil

Brasil

Produção in vitro de embriões de bovinos da raça Nelore oriundos de ovócitos de ovários com e sem corpo lúteo

In vitro production of Nelore bovine embryos originated from oocytes from ovaries with and without corpus luteum

Resumos

Datas de Publicação

Histórico