Diâmetro do folículo no momento da inseminação artificial em tempo fixo e taxa de concepção em vacas Nelore

Ribeiro Filho, Antonio de Lisboa; Ferraz, Priscila Assis; Rodrigues, Alexandra Soares; Bittencourt, Thereza Cristina Borio dos Santos Calmon; Loiola, Marcus Vinícius Galvão; Chalhoub, Marcos

doi:10.5216/cab.v14I4.19678

Resumos

Objetivou-se avaliar a influência do diâmetro do maior folículo no momento da IATF (DFOL) sobre a taxa de concepção de fêmeas Nelore submetidas ao protocolo de IATF. Para tanto, foram sincronizadas 348 fêmeas no dia 0 (D0) com um dispositivo intravaginal de progesterona (P4) associado a 2,0mg de benzoato de estradiol por via intramuscular (im). No dia 8 (D8), após a retirada da P4, administrou-se 500µg de Cloprostenol Sódico im, 0,6mg de cipionato de estradiol im e 300UI de gonadotrofina coriônica equina (eCG) im. No dia 10 (D10), antes de cada IATF, mensurou-se o DFOL de todos os animais por ultrassonografia transretal. O diagnóstico de gestação foi realizado 30 dias após a IATF por ultrassonografia transretal. Os dados foram avaliados pelo pacote estatístico SPSS (versão 19) e as médias do DFOL foram comparadas pelo teste t de Student. A taxa de concepção geral foi de 57,47% e a média geral do DFOL foi de 12,43±2,84mm. Houve diferença (P=0,0001) na comparação do DFOL entre as fêmeas gestantes (13,33±2,40mm) e as fêmeas não gestantes (11,27±2,75mm). Os resultados demonstram um efeito positivo do DFOL sobre a fertilidade de vacas Nelore submetidas a um protocolo de sincronização.

Bovinos; inseminação artificial; sincronização

The objective of this study was to evaluate the influence of the diameter of the largest follicle at the time of TAI (DFOL) on conception rate of Nellore cows submitted to TAI protocol. Therefore, 348 females were synchronized on day 0 (D0) with an intravaginal progesterone device (P4) associated with 2.0mg of estradiol benzoate intramuscularly (im). On day 8 (D8), after P4 removal, 500µg Sodium cloprostenol (im), 0.6mg of estradiol cypionate (im) and 300UI equine chorionic gonadotropin (eCG) (im) were administered. On day 10 (D10), immediately before each TAI, DFOL was measured in all animals by transrectal ultrasonography. Pregnancy diagnosis was performed 30 days after TAI by transrectal ultrasonography. Data were analyzed by SPSS (version 19) and DFOL means were compared by Student's t-test. The overall conception rate was 57.47% and DFOL overall average was 12.43±2.84mm. There were differences (P=0.0001) in the comparison of DFOL of pregnant (13.33±2.40mm) and nonpregnant (11.27±2.75mm) cows. The results showed a positive effect of DFOL on fertility of cows submitted to synchronization protocol.

Artificial insemination; cattle; synchronization

ATKINS, J. A.; SMITH, M. F.; WELLS, K. J.; GEARY, T. W. Factors affecting preovulatory follicle diameter and ovulation rate after gonadotrophin-releasing hormone in postpartum beef cows. Part I: cycling cows. Journal Animal Science, v.88, p. 2300-2310, 2010.
BARUSELLI, P. S.; SALES, J. N. S.; SALA, R. V.; VIEIRA, L. M.; SÁ FILHO, M. F. History, evolution and perspectives of timed artificial insemination programs in Brazil. Animal Reproduction v. 9, p. 139-152, 2012.
BARUSELLI, P. S.; REIS, E. L.; MARQUES, M. O.; NASSER, L. F.; BÓ, G. A. The use of hormonal treatments to improve reproductive performance of anestrous beef cattle in tropical climates. Animal Reproduction Science, v. 82-83, p. 479-486, 2004.
BARUSELLI, P. S.; MARQUES, M. O.; CARVALHO, N. A. T.; MADUREIRA, E. H.; CAMPOS FILHO, E. P. Efeito de diferentes protocolos de inseminação artificial em tempo fixo na deficiência reprodutiva de vacas de corte lactantes. Revista Brasileira de Reprodução Animal, v. 26, n. 3, p. 219-220, 2002.
BÓ, G. A.; BARUSELLI, P. S.; MARTINEZ, M. F. Pattern and manipulation of follicular development in Bos indicus cattle. Animal Reproduction Science, v. 78, p.307-326, 2003.
BÓ, G. A.; ADAMS, G.P; PIERSON, R. A.; MAPLETOFT, R. J. Exogenous control of follicular wave emergence in cattle. Theriogenology, v. 43, p. 31-40, 1995.
BORSATO, E. A.; LUDWIG JR., H. E.; RUBIN, K. C. P.; SAUT, J. P. E.; BARREIROS, T. R. R.; SENEDA, M. M. Relação entre o tamanho do folículo ovulatório e taxa de concepção em novilhas Bos taurus x Bos indicus submetidas a inseminação artificial em tempo fixo. Revista Brasileira de Reprodução Animal, v. 28, n. 3, p. 137-142, 2004.
BUTTER, S. A. A.; PHILLIPS, N. J.; BOE-HANSEN, G. B.; BO, G. A.; BURNS, B. M.; DAWSON, K.; MCGOWAN, M. R. Ovarian responses in Bos indicus heifers treated to synchronise ovulation with intravaginal progesterone releasing devices, oestradiol benzoate, prostaglandin F2α and equine chorionic gonadotrophin. Animal Reproduction Science, v. 129, p. 118-126, 2011.
CARVALHO, J. B. P.; CARVALHO, N. A. T.; REIS, E. L.; NICHI, M.; SOUZA, A. H.; BARUSELLI, P. S. Effect of early luteolysis in progesterone-based timed AI protocols in Bos indicus, Bos indicus x Bos taurus and Bos taurus heifers. Theriogenology, v. 69, p. 167-175, 2008.
DIAS, C. C.; WECHSLER, F. S.; DAY, M. L.; VASCONCELOS, J. L. M. Progesterone concentrations, exogenous equine chorionic gonadotropin, and timing of prostaglandin F2α treatment affect fertility in postpuberal Nelore heifers. Theriogenology, v. 72, p. 378-385, 2009.
HAWK, H. W. Sperm survival and transport in the female reproductive tract. Journal of Dairy Science, v. 77, p.2738-2744, 1983.
HOUGHTON, P.L.; LEMENAGER, R.P.; MOSS, G.E.; HENDRIX, K. S. Prediction of postpartum beef cow body composition using weight to height ratio and visual body condition score. Journal of Animal Science, v.68, p.1428 - 1437, 1990.
INFORZATTO, G. R.; SANTOS, W. R. M.; CLIMENI, B. S. O.; DELLALIBERA, F. L.; FILADELPHO, A. L. Emprego da IATF (Inseminação Artificial em Tempo Fixo) como alternativa na reprodução da pecuária de corte. Revista Científica Eletrônica de Medicina Veterinária, v. 11, p. 1-8, 2008.
GIMENES, L. U; SÁ FILHO, M. F.; CARVALHO, N. A. T.; TORRES-JUNIOR, J. R. S.; SOUZA, A. H.; MADUREIRA, E. H.; TRINCA, L. A.; SARTORELLI, E. S.; BARROS, C. M.; CARVALHO, J. B. P.; MAPLETOFT, R. J.; BARUSELLI, P. S. Follicle deviation and ovulatory capacity in Bos indicus heifers. Theriogenology, v. 69, p. 852-858, 2008.
LAMB, G. C.; STEVENSON, J. S.; KESLER, D. J.; GARVERICK, H. A.; BROWN, D. R.; SALFEN, B. E. Inclusion of an intravaginal progesterone insert plus GnRH and prostaglandin F2α for ovulation control in postpartum suckled beef cows. Journal Animal Science, v. 79, p. 2253-2259, 2001.
LARSON, J. E.; LAMB, G. C.; STEVENSON, J. S.; JOHNSON, S. K.; DAY, M. L.; GEARY, T. W.; KESLER, D. J.; DEJARNETTE, J. M.; SCHRICK, F. N.; DICOSTANZO A.; ARSENEAU, J. D. Synchronization of estrus in suckled beef cows for detected estrus and artificial insemination and timed artificial insemination using gonadotropin-releasing hormone, prostaglandin F2α, and progesterone. Journal of Animal Science, v. 84, p.332-342, 2006.
LONERGAN, P.; O`HARA, L.; FORDE, N. Papel da progesterona do diestro na função endometrial e desenvolvimento do concepto em bovinos. Animal Reproduction, v.10 , n. 3, p. 119-123, 2013.
MACMILLAN, K. L.; BURKE, C. R. Effects of oestrous cycle control on reproductive efficiency. Animal Reproduction Science, v. 42, p. 307-320, 1996.
MENEGHETTI, M.; SÁ FILHO, O. G.; PERES, R. F. G.; LAMB, G. C.; VASCONCELOS, J. L. M. Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows I: Basis for development of protocols. Theriogenology, v. 72, p. 179-189, 2009.
MANN, G. E.; LAMMING, G. E.; Relationship between maternal endocrine environment, early embryo development and inhibition of the luteolytic mechanism in cows. Reproduction, v. 121, p. 175-180, 2001.
PERES, R. F. G.; CLARO JUNIOR, I.; SÁ FILHO, O. G.; NOGUEIRA, G. P.; VASCONCELOS, J. L. M. Strategies to improve fertility in Bos indicus postpubertal heifers and nonlactating cows submitted to fixed-time artificial insemination. Theriogenology, v. 72, p. 681-689, 2009.
PERRY, G. A.; PERRY, B. L. Effect of preovulatory concentrations of estradiol and initiation of standing estrus on uterine pH in beef cows. Domestic Animal Endocrinology, v. 34, p. 333 - 338, 2008.
PERRY, G. A.; SMITH, M. F.; ROBERTS, A. J.; MACNEIL, M. D.; GREARY, T. W. Relationship between size of the ovulatory follicle and pregnancy success in beef heifers. Journal Animal Science, v. 85, p. 684-689, 2007.
PERRY, G. A.; SMITH, M. F.; LUCY, M. C.; GREEN, J. A.; PARKS, T. E.; MACNEIL, M. D.; ANDREW, J. R.; THOMAS, W. G. Relationship between follicle size at insemination and pregnancy success. The National Academy of Sciences of the USA, v. 102, p. 5268-5273, 2005.
SÁ FILHO, M. F.; SANTOS, J. E. P.; FERREIRA, R. M.; SALES, J, N. S.; BARUSELLI, P. S. Importance of estrus on pregnancy submitted to estradiol/progesterone- based timed insemination protocols. Theriogenology, v. 76, p.455-463, 2011.
SÁ FILHO, M. F.; CRESPILHO, A. M.; SANTOS, J. E. P.; PERRY, G. A.; BARUSELLI, P. S. Ovarian follicle diameter at timed insemination and estrous response influence likelihood of ovulation and pregnancy after estrous synchronization with progesterone or progestin-based protocols in suckled Bos indicus cows. Animal Reproduction Science, v. 120, p. 23-30, 2010.
SÁ FILHO, O. G.; MENEGHETTI, M.; PERES, R. F. G.; LAMB, G. C.; VASCONCELOS, J. L. M. Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows II: Strategies and factors affecting fertility. Theriogenology, v. 72, p. 210-218, 2009a.
SÁ FILHO, O. G.; THATCHER, W. W.; VASCONCELOS, J. L. M. Effect of progesterone and/or estradiol treatments prior to induction of ovualation on subsequent luteal lifespan in anestrous Nelore cows. Animal Reproduction Science, v. 112, p. 95-106, 2009b.
SANTOS, J. E. P.; THATCHER, W.W.; CHEBEL, R. C.; CERRI, R. L. A.; GALVÃO, K. N; The effect of embryonic death rates in cattle on the efficacy of estrus synchronization programs. Animal Reproduction Science, v. 82 - 83, p. 513 - 535, 2004.
SIMÕES, R. A. L.; SATRAPA, R. A.; ROSA, F. S.; PIAGENTINI, M.; CASTILHO, A. C. S.; ERENO, R. L.; TRINCA, L. A.; NOGUEIRA, M. F. G.; BURATINI JR, J.; BARROS, C. M. Ovulation rate and its relationship with follicle diameter and gene expression of the LH receptor (LHR) in Nelore cows. Theriogenology, v. 77, p.139-147, 2012.
SIQUEIRA, L. G.; TORRES, C. A. A.; AMORIN, L. S.; SOUZA, E. D.; CAMARGO, L. S. A.; FERNANDES, C. A. C.; VIANA, J. H. M. Interrelationships among morphology, echtexture, and function of the bovine corpus luteum during the estrous cycle. Animal Reproduction Science v. 115, p. 18-28, 2009.
VASCONCELOS, J. L. M.; SARTORI, R.; OLIVEIRA, H. N.; GUINTHER, J. G.; WILTBANK, M. C. Reduction in size of the ovulatory follicle reduces subsequent luteal size and pregnancy rate. Theriogenology, v. 56, p. 307-314, 2001.

Datas de Publicação

Publicação nesta coleção
13 Jan 2014
Data do Fascículo
Dez 2013

Histórico

Recebido
15 Ago 2012
Aceito
11 Set 2013

This work is licensed under a Creative Commons Attribution 4.0 International License.

[1] ATKINS, J. A.; SMITH, M. F.; WELLS, K. J.; GEARY, T. W. Factors affecting preovulatory follicle diameter and ovulation rate after gonadotrophin-releasing hormone in postpartum beef cows. Part I: cycling cows. Journal Animal Science, v.88, p. 2300-2310, 2010.

[2] BARUSELLI, P. S.; SALES, J. N. S.; SALA, R. V.; VIEIRA, L. M.; SÁ FILHO, M. F. History, evolution and perspectives of timed artificial insemination programs in Brazil. Animal Reproduction v. 9, p. 139-152, 2012.

[3] BARUSELLI, P. S.; REIS, E. L.; MARQUES, M. O.; NASSER, L. F.; BÓ, G. A. The use of hormonal treatments to improve reproductive performance of anestrous beef cattle in tropical climates. Animal Reproduction Science, v. 82-83, p. 479-486, 2004.

[4] BARUSELLI, P. S.; MARQUES, M. O.; CARVALHO, N. A. T.; MADUREIRA, E. H.; CAMPOS FILHO, E. P. Efeito de diferentes protocolos de inseminação artificial em tempo fixo na deficiência reprodutiva de vacas de corte lactantes. Revista Brasileira de Reprodução Animal, v. 26, n. 3, p. 219-220, 2002.

[5] BÓ, G. A.; BARUSELLI, P. S.; MARTINEZ, M. F. Pattern and manipulation of follicular development in Bos indicus cattle. Animal Reproduction Science, v. 78, p.307-326, 2003.

[6] BÓ, G. A.; ADAMS, G.P; PIERSON, R. A.; MAPLETOFT, R. J. Exogenous control of follicular wave emergence in cattle. Theriogenology, v. 43, p. 31-40, 1995.

[7] BORSATO, E. A.; LUDWIG JR., H. E.; RUBIN, K. C. P.; SAUT, J. P. E.; BARREIROS, T. R. R.; SENEDA, M. M. Relação entre o tamanho do folículo ovulatório e taxa de concepção em novilhas Bos taurus x Bos indicus submetidas a inseminação artificial em tempo fixo. Revista Brasileira de Reprodução Animal, v. 28, n. 3, p. 137-142, 2004.

[8] BUTTER, S. A. A.; PHILLIPS, N. J.; BOE-HANSEN, G. B.; BO, G. A.; BURNS, B. M.; DAWSON, K.; MCGOWAN, M. R. Ovarian responses in Bos indicus heifers treated to synchronise ovulation with intravaginal progesterone releasing devices, oestradiol benzoate, prostaglandin F2α and equine chorionic gonadotrophin. Animal Reproduction Science, v. 129, p. 118-126, 2011.

[9] CARVALHO, J. B. P.; CARVALHO, N. A. T.; REIS, E. L.; NICHI, M.; SOUZA, A. H.; BARUSELLI, P. S. Effect of early luteolysis in progesterone-based timed AI protocols in Bos indicus, Bos indicus x Bos taurus and Bos taurus heifers. Theriogenology, v. 69, p. 167-175, 2008.

[10] DIAS, C. C.; WECHSLER, F. S.; DAY, M. L.; VASCONCELOS, J. L. M. Progesterone concentrations, exogenous equine chorionic gonadotropin, and timing of prostaglandin F2α treatment affect fertility in postpuberal Nelore heifers. Theriogenology, v. 72, p. 378-385, 2009.

[11] HAWK, H. W. Sperm survival and transport in the female reproductive tract. Journal of Dairy Science, v. 77, p.2738-2744, 1983.

[12] HOUGHTON, P.L.; LEMENAGER, R.P.; MOSS, G.E.; HENDRIX, K. S. Prediction of postpartum beef cow body composition using weight to height ratio and visual body condition score. Journal of Animal Science, v.68, p.1428 - 1437, 1990.

[13] INFORZATTO, G. R.; SANTOS, W. R. M.; CLIMENI, B. S. O.; DELLALIBERA, F. L.; FILADELPHO, A. L. Emprego da IATF (Inseminação Artificial em Tempo Fixo) como alternativa na reprodução da pecuária de corte. Revista Científica Eletrônica de Medicina Veterinária, v. 11, p. 1-8, 2008.

[14] GIMENES, L. U; SÁ FILHO, M. F.; CARVALHO, N. A. T.; TORRES-JUNIOR, J. R. S.; SOUZA, A. H.; MADUREIRA, E. H.; TRINCA, L. A.; SARTORELLI, E. S.; BARROS, C. M.; CARVALHO, J. B. P.; MAPLETOFT, R. J.; BARUSELLI, P. S. Follicle deviation and ovulatory capacity in Bos indicus heifers. Theriogenology, v. 69, p. 852-858, 2008.

[15] LAMB, G. C.; STEVENSON, J. S.; KESLER, D. J.; GARVERICK, H. A.; BROWN, D. R.; SALFEN, B. E. Inclusion of an intravaginal progesterone insert plus GnRH and prostaglandin F2α for ovulation control in postpartum suckled beef cows. Journal Animal Science, v. 79, p. 2253-2259, 2001.

[16] LARSON, J. E.; LAMB, G. C.; STEVENSON, J. S.; JOHNSON, S. K.; DAY, M. L.; GEARY, T. W.; KESLER, D. J.; DEJARNETTE, J. M.; SCHRICK, F. N.; DICOSTANZO A.; ARSENEAU, J. D. Synchronization of estrus in suckled beef cows for detected estrus and artificial insemination and timed artificial insemination using gonadotropin-releasing hormone, prostaglandin F2α, and progesterone. Journal of Animal Science, v. 84, p.332-342, 2006.

[17] LONERGAN, P.; O`HARA, L.; FORDE, N. Papel da progesterona do diestro na função endometrial e desenvolvimento do concepto em bovinos. Animal Reproduction, v.10 , n. 3, p. 119-123, 2013.

[18] MACMILLAN, K. L.; BURKE, C. R. Effects of oestrous cycle control on reproductive efficiency. Animal Reproduction Science, v. 42, p. 307-320, 1996.

[19] MENEGHETTI, M.; SÁ FILHO, O. G.; PERES, R. F. G.; LAMB, G. C.; VASCONCELOS, J. L. M. Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows I: Basis for development of protocols. Theriogenology, v. 72, p. 179-189, 2009.

[20] MANN, G. E.; LAMMING, G. E.; Relationship between maternal endocrine environment, early embryo development and inhibition of the luteolytic mechanism in cows. Reproduction, v. 121, p. 175-180, 2001.

[21] PERES, R. F. G.; CLARO JUNIOR, I.; SÁ FILHO, O. G.; NOGUEIRA, G. P.; VASCONCELOS, J. L. M. Strategies to improve fertility in Bos indicus postpubertal heifers and nonlactating cows submitted to fixed-time artificial insemination. Theriogenology, v. 72, p. 681-689, 2009.

[22] PERRY, G. A.; PERRY, B. L. Effect of preovulatory concentrations of estradiol and initiation of standing estrus on uterine pH in beef cows. Domestic Animal Endocrinology, v. 34, p. 333 - 338, 2008.

[23] PERRY, G. A.; SMITH, M. F.; ROBERTS, A. J.; MACNEIL, M. D.; GREARY, T. W. Relationship between size of the ovulatory follicle and pregnancy success in beef heifers. Journal Animal Science, v. 85, p. 684-689, 2007.

[24] PERRY, G. A.; SMITH, M. F.; LUCY, M. C.; GREEN, J. A.; PARKS, T. E.; MACNEIL, M. D.; ANDREW, J. R.; THOMAS, W. G. Relationship between follicle size at insemination and pregnancy success. The National Academy of Sciences of the USA, v. 102, p. 5268-5273, 2005.

[25] SÁ FILHO, M. F.; SANTOS, J. E. P.; FERREIRA, R. M.; SALES, J, N. S.; BARUSELLI, P. S. Importance of estrus on pregnancy submitted to estradiol/progesterone- based timed insemination protocols. Theriogenology, v. 76, p.455-463, 2011.

[26] SÁ FILHO, M. F.; CRESPILHO, A. M.; SANTOS, J. E. P.; PERRY, G. A.; BARUSELLI, P. S. Ovarian follicle diameter at timed insemination and estrous response influence likelihood of ovulation and pregnancy after estrous synchronization with progesterone or progestin-based protocols in suckled Bos indicus cows. Animal Reproduction Science, v. 120, p. 23-30, 2010.

[27] SÁ FILHO, O. G.; MENEGHETTI, M.; PERES, R. F. G.; LAMB, G. C.; VASCONCELOS, J. L. M. Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows II: Strategies and factors affecting fertility. Theriogenology, v. 72, p. 210-218, 2009a.

[28] SÁ FILHO, O. G.; THATCHER, W. W.; VASCONCELOS, J. L. M. Effect of progesterone and/or estradiol treatments prior to induction of ovualation on subsequent luteal lifespan in anestrous Nelore cows. Animal Reproduction Science, v. 112, p. 95-106, 2009b.

[29] SANTOS, J. E. P.; THATCHER, W.W.; CHEBEL, R. C.; CERRI, R. L. A.; GALVÃO, K. N; The effect of embryonic death rates in cattle on the efficacy of estrus synchronization programs. Animal Reproduction Science, v. 82 - 83, p. 513 - 535, 2004.

[30] SIMÕES, R. A. L.; SATRAPA, R. A.; ROSA, F. S.; PIAGENTINI, M.; CASTILHO, A. C. S.; ERENO, R. L.; TRINCA, L. A.; NOGUEIRA, M. F. G.; BURATINI JR, J.; BARROS, C. M. Ovulation rate and its relationship with follicle diameter and gene expression of the LH receptor (LHR) in Nelore cows. Theriogenology, v. 77, p.139-147, 2012.

[31] SIQUEIRA, L. G.; TORRES, C. A. A.; AMORIN, L. S.; SOUZA, E. D.; CAMARGO, L. S. A.; FERNANDES, C. A. C.; VIANA, J. H. M. Interrelationships among morphology, echtexture, and function of the bovine corpus luteum during the estrous cycle. Animal Reproduction Science v. 115, p. 18-28, 2009.

[32] VASCONCELOS, J. L. M.; SARTORI, R.; OLIVEIRA, H. N.; GUINTHER, J. G.; WILTBANK, M. C. Reduction in size of the ovulatory follicle reduces subsequent luteal size and pregnancy rate. Theriogenology, v. 56, p. 307-314, 2001.

Brasil

Brasil

Diâmetro do folículo no momento da inseminação artificial em tempo fixo e taxa de concepção em vacas Nelore

Follicle diameter at fixed-time artificial insemination and conception rate in Nellore cows

Resumos

Datas de Publicação

Histórico