Pregnancy rates in criollo breed mares after ultrasound-guided follicular aspiration

Taxa de gestação em éguas da raça crioula após aspiração folicular guiada por ultrassom

Abstracts

There are few studies about transvaginal ultrasound-guided follicle aspiration in equine medicine regarding potential complications to future fertility of aspirated mares. In order to evaluate the effect of follicular aspiration on subsequent fertility in mares, two experiments were conducted. In Experiment I, fifteen Criollo mares were allocated to one of three groups according to the diameter of the aspirated follicle during estrus: 25-29mm (n=4; Group 1); 30-34mm (n=6; Group 2); > 35mm (n=5; Group 3) and control group (n=15; Group 4). In Experiment II, the follicular aspiration was attempted in twenty-five mares during diestrous, when at least four follicles (> 5mm) were seen in the transrectal ultrasonography of both ovaries. All visible follicles, between 4 and 8 mm, were aspirated. Thirty-one mares served as control. In Experiments I and II, the pregnancy rates in the following cycle after aspiration were 75.0% (Group 1), 83.3% (Group 2), 60.0% (Group 3), and 73.3% (Group 4 - Control); and 76.0% in the aspirated diestrous group and 77.4% in the control group (non aspirated), respectively. On both experiments, pregnancy rates were similar (P>0.05) in treated and control mares. The results of this study show that the conception rates of the first estrus period following follicular aspiration are not affected by the procedure.

equine; fertility; follicular aspiration; mares

Há poucos estudos sobre aspiração folicular transvaginal guiada por ultrassom na medicina equina abordando complicações futuras na fertilidade das éguas aspiradas. Com o objetivo de avaliar o efeito da aspiração folicular na fertilidade das éguas, foram conduzidos dois experimentos. No experimento I, 15 éguas da raça Crioula foram distribuídas em três grupos de acordo com o diâmetro do folículo aspirado durante o estro: 25-29mm (n=4; grupo 1); 30-34mm (n=6; grupo 2); > 35mm (n=5; grupo 3) e grupo controle (n=15; grupo 4). No experimento II, a aspiração folicular foi realizada em 25 éguas durante o diestro quando pelo menos 4 folículos (>5mm) foram observados na ultrassonografia transretal em ambos os ovários. Foram aspirados todos os folículos visíveis, entre 4 e 8 mm. Trinta e uma éguas serviram como controle. No experimento I, a taxa de prenhez no ciclo seguinte a aspiração foi de 75% (grupo 1), 83,3% (grupo 2), 60% (grupo 3), e 73,3% (grupo 4). No experimento II foi de 76% no grupo aspirado e 77,4% no grupo controle (não aspirado). Em ambos os experimentos, as taxas de prenhez foram similares (P>0,05). Os resultados mostram que a taxa de concepção no primeiro ciclo após a aspiração folicular não é afetada pelo procedimento.

Aspiração folicular; éguas; equino; fertilidade

• ¹
  Carnevale EM, Ginther OJ. Use of a linear ultrasonic transducer for the transvaginal aspiration and transfer oocytes in the mare. J. Equine Vet. Sci. 1993, 13(6): 331-333. Available in: http://www.sciencedirect.com/science/article/pii/S0737080606811184#
• ²
  Brück I, Raun K, Synnestvedt B, et al Follicle aspiration in the mare using a transvaginal ultrasound - guided technique. Equine Vet. J. 1992, 24(1): 58-59. Available in: http://www.ncbi.nlm.nih.gov/pubmed/1555542
• ³
  Gaetano M, Barbara M, Eleonora I, et al Fertility in the mare after repeated transvaginal ultrasound-guided aspirations. Anim. Reprod. Sci. 2005, 88(3/4): 299-308. Available in: http://www.sciencedirect.com/science/article/pii/S0378432005000254
• ⁴
  Mozzaquatro FD, Verstegen JP, Douglas RH, et al Luteal function induced by transvaginal ultrasonic-guided follicular aspiration in mares. Anim. Reprod. Sci. 2010, 119(1/2): 56-62. Available in: http://www.sciencedirect.com/science/article/pii/S0378432009003042
• ⁵
  Mozzaquatro FD, Verstegen JP, Douglas RH, et al Progesterone production in mares and echographic evaluation of the corpora lutea formed after follicular aspiration. Reprod. Dom. Anim. 2012, 47(2): 288-292. Available in: http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0531.2011.01856.x/pdf
• ⁶
  Bennett SJ, Waterstone JJ, Cheng WC, et al Complications of transvaginal ultrasound-directed follicle aspiration: a review of 2670 consecutive procedures. J. Assist. Reprod. Genet. 1993, 10(1): 72-77. Available in: http://www.ncbi.nlm.nih.gov/pubmed/8499683
• ⁷
  Dicker D, Ashkenazi J, Feldberg D, et al Severe abdominal complications after transvaginal ultrasonographically guided retrieval of oocytes for in vitro fertilization and embryo transfer. Fertil. Steril. 1993, 59(6): 1313-1315. Available in: http://www.ncbi.nlm.nih.gov/pubmed/8495784
• ⁸
  Weber JA, Causey RC, Emmans EE. Induction of luteolysis in mares by ultrasound-guided intraluteal treatment with PGF2α. Theriogenology. 2001, 55(9): 1769-1776. Available in: http://ac.els-cdn.com/S0093691X01005192/1-s2.0-S0093691X01005192-main.pdf?_tid=b0e1bf04-39ee-11e4-80b4-00000aab0f02&acdnat=1410465986_dcb5ec207278430066d159ccbc705dba
• ⁹
  Vanderwall DK, Woods GL. Severe internal hemorrhage resulting from transvaginal ultrasound-guided follicle aspiration in a mare. J. Equine Vet. Sci. 2002, 22(2): 84-86. Available in: http://ac.els-cdn.com/S0737080602700944/1-s2.0-S0737080602700944-main.pdf?_tid=064c2f42-39ef-11e4-b3f2-00000aab0f01&acdnat=1410466129_67b3aa88431392f1a70c274621a0d28d
• ¹⁰
  Velez IC, Arnold C, Jacobson CC, et al Effects of repeated transvaginal aspiration of immatures follicles on mare health and ovarian status. Equine Vet. J. 2012, 44: 78-83. Available in: http://www.deepdyve.com/lp/wiley/effects-of-repeated-transvaginal-aspiration-of-immature-follicles-on-Xcix1Ms1pk
• ¹¹
  Silva CAM, Barros SS., Esquerre RA, et al A biópsia endometrial na avaliação da fertilidade na égua. Pesq. Vet. Bras. 1987, 7(4): 131-133. Available in: http://www.scielo.br/scielo.php?script=sci_nlinks&ref=000112&pid=S0100-736X200700120000700030&lng=pt
• ¹²
  Kenney RM. Cyclic and pathologic changes of the mare endometrium as detected by biopsy, with a note on early embryonic death. J. Am. Vet. Med. Assoc. 1978,172(3): 241-262. Available in: http://www.ncbi.nlm.nih.gov/pubmed/621166
• ¹³
  Goudet G, Bézard J, Duchamp G, et al Equine oocyte competence for nuclear and cytoplasmic in vitro maturation: effect of follicle size and hormonal environment. Biol. Reprod. 1997, 57(2): 232-245. Available in: http://www.biolreprod.org/content/57/2/232.long

Publication Dates

• Publication in this collection
  15 Oct 2014
• Date of issue
  Sept 2014

History

• Received
  10 Sept 2013
• Accepted
  10 Sept 2014