SciELO - Scientific Electronic Library Online

 
vol.20IMPLICATIONS OF Erythrina crista-galli EXTRACT AS ANTISIOLYTIC FOR Carassius auratusSODIUM CONTENT IN CHEESES: SUITABILITY TO THE VOLUNTARY AGREEMENT AND LABELING author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Ciência Animal Brasileira

Print version ISSN 1518-2797On-line version ISSN 1809-6891

Ciênc. anim. bras. vol.20  Goiânia  2019  Epub Mar 18, 2019

http://dx.doi.org/10.1590/1809-6891v20e-43549 

CIÊNCIA E TECNOLOGIA DE ALIMENTOS

LACTOSE HYDROLYSIS AND ORGANIC ACIDS PRODUCTION IN YOGURT PREPARED WITH DIFFERENT ONSET TEMPERATURES OF ENZYMATIC ACTION AND FERMENTATION

HIDRÓLISE DA LACTOSE E PRODUÇÃO DE ÁCIDOS ORGÂNICOS EM IOGURTES ELABORADOS COM DIFERENTES TEMPERATURAS DE INICIO DE AÇÃO ENZIMÁTICA E FERMENTAÇÃO

Raphael Ferreira Barros1  * 
http://orcid.org/0000-0002-3605-3716

Camila Sampaio Cutrim1 
http://orcid.org/0000-0002-7109-760X

Marion Pereira da Costa1 
http://orcid.org/0000-0002-3003-6763

Carlos Adam Conte Junior1 
http://orcid.org/0000-0001-6133-5080

Marco Antonio Sloboda Cortez1 
http://orcid.org/0000-0003-0984-2338

1Universidade Federal Fluminense, Niterói, RJ, Brazil

Abstract

The aim of this study was to evaluate lactose hydrolysis between two different initial temperatures of enzymatic and fermentation action to improve the fermentation period by checking the components formed and hydrolysis levels of lactose by HPLC. pH and titratable acidity analysis between treatments - higher initial temperature (HIT), 42 ºC, and lower initial temperature (LIT), 30 ºC - were different (P<0.05) during the fermentation process. HIT obtained lower pH and higher titratable acidity values as 4.6±0.04 and 0.73±0.01 g lactic acid.100 mL-1, respectively, against 4.82±0.01 and 0.64±0.01 g lactic acid.100 mL-1 from LIT. Lactose content was different (P<0.05) between treatments while fermenting; however, at the end of the fermentation, it reached 4.565±0.34 mg.mL-1 and 4.398±0.18 mg.mL-1 for LIT and HIT (P>0,05), respectively. Glucose and galactose remained buoyant during the fermentation period, which indicates its production and consumption at the same time by the lactic acid bacteria involved. The lactic acid concentration in LIT was higher (P<0.05) (18.64±0.62 mg.mL-1) than in HIT (17.56±0.53 mg.mL-1) although citric and acetic acids content decreased throughout the process. In conclusion, the lactase enzyme contributed to reduce the lactose content without affecting the fermentation process. In addition, both treatments obtained lower values of lactose, which is sufficient for the consumption by those who have lactose malabsorption.

Keywords: β-galactosidase; fermented milk; functional food; HPLC

Resumo

O objetivo deste estudo foi avaliar a hidrólise da lactose em relação a duas diferentes temperaturas iniciais de ação enzimática e fermentação, a fim de melhorar o período de fermentação, verificando a formação de componentes e o nível de hidrolise por CLAE (HPLC). Os resultados das análises de pH e acidez titulável entre os tratamentos com maior temperatura inicial (HIT), 42 ºC, e menor temperatura inicial (LIT), 30 ºC, foram diferentes (P<0,05) durante a fermentação. HIT obteve menores valores de pH e maiores valores de acidez titulável como 4,6±0,04 e 0,73±0,01 g acido láctico.100 mL-1, respectivamente, contra 4,82±0,01 e 0,64±0,01 g acido láctico.100 mL-1 de LIT. O teor de lactose difereriu (P <0,05) entre os tratamentos durante a fermentação; entretanto, no final do processo alcançaram 4,40±0,14 mg.mL-1 e 4,32±0,14 mg.mL-1 para LIT e HIT (P>0,05), respectivamente. Teores de glicose e galactose mantiveram-se dinâmicos. A concentração de ácido láctico em LIT (P<0,05) foi maior (18,64±0,62 mg.mL-1) que em HIT (17,56±0,53 mg.mL-1), embora o teor dos ácidos cítrico e acético tenha diminuído durante o processo. Assim, pode-se concluir que a enzima lactase contribuiu para reduzir o teor de lactose, sem influenciar o processo de fermentação. Além disso, ambos os tratamentos obtiveram valores mais baixos de lactose, o que é suficiente para o consumo por pessoas com intolerância a lactose.

Palavras-chave: β-galactosidase; alimento funcional; CLAE; leite fermentado; HPLC

Introduction

Milk is considered a complete food: source of protein (3.2 w/w); carbohydrates, particularly lactose, (4.7 % w/w), fat (3.6 % w/w); minerals (0.6 w/w); and vitamins. Due to the essential nutrients and the high digestibility (bioavailability) of nutrients, milk and dairy products are considered highly nutritious food(1). Lactose is the main carbohydrate in milk and is formed by one molecule of α-D-glucose and β-D-galactose. In cow’s milk, lactose concentration is around 4.8%, which means 50 to 52% of total solids(2-3). However, this lactose percentage can be detrimental to subjects with impaired or completely absent intestinal lactase activity (lactose malabsorption)(4).

Lactose malabsorption, also known as lactose non-persistent, is due to poor digestion of this carbohydrate(5), causing gastrointestinal symptoms (lactose intolerance)(4). Researchers have shown that more than 65% of the world's population - mostly adults - has a certain level of this disorder. Raithel(6) reported that lactose malabsorption has two different sources: a primary deficiency that includes physiological lactase reduction and autosomal recessive familial lactase deficiency; and a secondary deficiency featuring inflammatory intestinal diseases. Therefore, various technologies are employed for the development of reduced-lactose products, such as milk fermentation and enzymatic hydrolysis.

According to Codex Standards for Fermented Milk(7), yogurt is a fermented milk product by homofermentative acid lactic bacteria. In fermentation process, milk lactose is fermented by specific acid lactic cultures as Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus culminated mainly lactic acid production at the end of the metabolism chain(8-9). Although there is a certain degree of lactose consumption by yogurt starter culture, a considerable amount of lactose remains intact in yogurt(3).

Yogurt presents about 30% less lactose than milk; however, it is still considered relatively high for some lactose malabsorption levels(10). Only the milk fermentation process may not be not sufficient for people who have some level of lactose intolerance; therefore, one of the solutions for such cases is to elaborate a dairy product with reduced lactose or even lactose free, as the ones obtained with fermented milk added of β-galactosidase enzyme. Enzyme use in the manufacture of low-lactose dairy products is nowadays an usual practice(11).

For these reasons, the hydrolysis conditions of milk lactose by the β-galactosidase enzyme action and the fermentation conditions by S. thermophilus and L. delbrueckii subsp. bulgaricus were compared between two treatments with different initial temperatures: low initial temperature (25 ºC) and high initial temperature (40 ºC). Therefore, the aim of this study was to evaluate lactose hydrolysis between two different initial temperatures of enzymatic and fermentation action. Components formation and hydrolysis levels of lactose were also checked, as well as whether the process obtained a low lactose concentration as required by humans with lactose malabsorption.

Materials and Methods

Commercial cow’s whole UHT milk (Ninho®, Nestle, São Paulo, Brazil), was used to elaborate the fermented milk. The milk was acquired in the retail market, transported and stored at room temperature. A whole milk sample was analyzed to indicate its physicochemical quality, determinate titratable acidity (Official Method 947.05), and pH (Official Method 973.41)(12). The pH was determined by inserting a pH probe into milk (pH meter PG1800, CapLab, São Paulo, Brazil) after calibrating it with fresh pH 4.0 and 7.0 standard buffers.

To produce the yogurt, the dosage of 50 U of thermophilic lactic acid culture (Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus strains YF L-812 CHR HANSEN®) to 500 L of milk was used, as suggested by the supplier, aiming at a cellular count of 10(11) CFU.g-1 of product

The β-galactosidase enzyme used in this research to hydrolyze the lactose was Maxilact Lx 5000 (DSM Food Specialties, Delft, Netherlands), which is a refined lactase formulation derived from dairy yeast Kluyveromyces lactis. The dosage used was 0.45 mL of enzyme per liter of milk, as suggested by the supplier. The enzyme was added into milk and stirred at the start of fermentation in both treatments. The lactose hydrolysis and fermentation process were performed at an oven at 41 ± 1 °C during fermentation time.

Yogurt was made by applying the improved traditional method (Figure 1) adapted to laboratory scale(3). It was prepared with whole milk, into two separated groups, using 3 L for each treatment. The first group, Low Initial Temperature (LIT), was prepared with milk at the temperature of 30 °C and the second one, High Initial Temperature (HIT), was prepared with milk at the temperature of 42 °C. In both groups a single-stage process took place with the simultaneous addition of β-galactosidase enzyme and lactic acid culture, using the dosages recommended by the respective manufacturers (as reported above). The fermentation was performed in an oven at 42 °C for 5 hours (300 minutes); thus, after the first hour, both temperatures were the same. The process was interrupted when the pH reached 4.7 ± 0.1. After that, the yogurt was cooled immediately in a refrigerator at 4 °C.

Figure 1 Scheme illustrating a method for yogurt poduction. 

During fermentation, the yogurt was analyzed every each hour to determinate pH and titratable acidity(12). In addition, the yogurt was also analyzed to determinate carbohydrates and organics acids, which was extracted and carried out as following described.

The carbohydrates and organics acids extraction was carried out using a modification of the method described by González de Llano et al.(13), performed in triplicate. Five milliliters of 45 mMol H2SO4 were added onto 1 mL of each sample and homogenized by vortexing for one minute. After that, all samples remained under agitation for one hour in a shaker table (TS - 2000 A VDRL shaker, Biomixer®) and then they were centrifugated at 5500 g for 30 minutes at 4 °C. Finally, the supernatant was filtered through Whatman no. 1 filter paper (Sigma-Aldrich, St. Louis, MO, USA).

Filtered samples were injected (20 µL) in triplicate into an HPLC system consisted of a LC/20 AT pump integrated with CBM-20A and equipped with SPD-M20A diode array and refractive index RID-10A detectors (Shimadzu Corp., Tokyo, Japan). Carbohydrates and lactic acid separations were performed with an HPX-87H 300 x 7.8 mm Aminex cation-exchange column (Bio-Rad, Hercules, CA, USA), maintained at 60 ºC. The mobile phase used was 3 mM H2SO4 at isocratic flow rate at 0.5 mL.min-1. Chromatograms from HPLC and compound quantification were obtained using the LC Solution software (Shimadzu Corp., Tokyo, Japan). Calibration curves were made from standard solutions prepared in Milli-Q water (Millipore, Billerica, MA, USA). The interest peaks were identified by comparing retention times of the standards with the samples. The concentration of each samples components was determined from the area of individual peaks. Carbohydrates identification was performed by refractive index detector while lactic acid by using a diode array detector model monitoring the absorbance at 210 nm.

One-way ANOVA with repeated measures of analytical methods was used to identify differences between pH values, titratable values, organic acids, and carbohydrates content over the yoghurt fermentation, as well as Tukey’s test. Statistical significance was set at a 0.05 level of confidence. All analyses were performed using commercially available statistical package XLSTAT 2013.2.03 (Addinsoft, Paris, France).

Results

Milk physicochemical analyses results are presented in Table 1. Regarding hydrolysis and fermentation processes, the pH reached at the end of fermentation (4.82 ± 0.01 for LIT and 4.6 ± 0.04 for HIT) was considered a P< 0.05 among the groups (Table 2). Moreover, the titratable acidity presented a similar result with HIT (0.73 g lactic acid.mL-1), higher (P<0.05) than LIT (0.64 g lactic acid.mL-1), as seen in Table 3.

Table 1 Characterization of UHT milk samples regarding pH, titratable acidity, carbohydrates, and organic acids 

Analysis Unit Results
Carbohydrates
(HPLC)
Lactose (mg-tnL1) 56.29±1.009
Glucose (mg.mL1) 0.16±0.007
Galactose (mg.mL1) 0.89±0.044
Lactic acid (mg.mL1) 0.15±0.158
Organic acids (HPLC) Acetic acid (mg.mL1) 13.52±2.958
Citric acid (mg.mL1) 14.24±0.757
pH - 6.58
Titratable acidity (g lactic acid .100mL1) 0.16

Table 2 pH determination values during the whole five hours (300 minutes) of milk fermentation process 

Group Fermentation lime (min)
0 60 120 180 240 300
LIT 6.54±0.02Aa 6.34±0.01Ab 6.13±0.01Ac 5.38±0.01Ad 4.96±0.03Ae 4.82±0.01Af
HIT 6.52±0.01Aa 5.95±0.02Bb 5.36*0.01Bc 4.97±0.02Bd 4.69±0.02Be 4.60±0.04Bf

A-DCapital letters in the columns indicate significant differences among the different treatment, P < 0.05.

a-dLowercase letters in rows indicate significant differences among different times the treatment, P < 0.05.

Table 3 Titratable acidity values measures during the whole five hours (300 minutes) of milk fermentation process 

Group Fermentation time (minutes)
0 60 120 180 240 300
LIT 0.16Aa 0.20Bb 0.29±0.01Bc 0.45±0.01Bd 0.55±0.01Be 0.64±0.01Bf
HIT 0.16Aa 0.27±0.01Ab 0.47±0.01Ac 0.60±0.01Ad 0.67±0.01Ae 0.73±0.01Af

A-DCapital letters in the columns indicate significant differences among the different treatment, P < 0.05.

a-dLowercase letters in rows indicate significant differences among different times the treatment, P < 0.05.

Lactose concentration started at 56.29±1.009 mg.mL-1. On the first four hours, HIT kept the lactose content P<0.05 lower than LIT. Nevertheless, at the end of 300 minutes, the concentrations reached were 4.565 ± 0.34 mg.mL-1 and 4.398 ± 0.18 mg.mL-1 of lactose for LIT and HIT (Table 4), respectively. Hence, with simultaneous fermentation process and lactose enzymatic hydrolysis, this compound decreased in both treatments at the end of fermentation (P>0.05).

Table 4 Carbohydrates and organic acids behavior found in preheat treatment during milk fermentation process 

Chemical
compounds
(g.100 ml-1)
Fermentation Time (minutes)
0 60 120 180 240 300
LIT HIT LIT HIT LIT HIT LIT HIT LIT IHT LIT HIT
Lactose 56.29±2.78Aa 52.90±5.42Aa 24.24±1.24Ab 5.73±0.04Bc 5.84±0.11®Bc 5.97±0.09Ab 5.69±0.04®Bcd 5.78±0.08Abc 4.80±0.25Adc 4.71±0.22-Ad 4.40±0.14Ac 4.32±0.14Ac
Glucose 0.16±0.01Af 0.16±0.01Af 48.94±2.20Aa 33.18±0.24®Ba 41.05±1.75Ab 27.33±0.81®Bb 32.51±1.50Ac 25.97±0.90Bc 29.94±0.38Ad 19.97±0.30Be 26.01±1.13Ae 21.72±0.75Bd
Galactose 0.89±0.04 Ad 0.89±0.04 Ae 23.78±1.52Aa 17.61±0.19Ba 23.82±1.08Aa 15.88Bb±0.17 20.11Ab±0.92 16.23Bb±0.62 19.95Ab±0.27 13.30Bb±0.17 18.09Ac±0.82 11.71Bc±0.25
Lactic Acid 0.15±0.02Ae 0.15±0.02Ae 3.18±1.03Bd 7.13±0.09Ad 10.88±0.44®Bc 13.01±0.44Ac 15.15±0.87Bd 17.40±0.63Aa 18.33±0.21Aa 15.42±0.10®Bd 18.64±0.62Aa 17.56±0.53®Ba
Acetic Acid 17.98±1.43Aa 11.15±0.48Ba 16.42±0.87Ab 11.42±0.18Da 15.03±0.51Ab 10.52±0.34Dbc 13.37±0.76Ac 10.86±0.39Dab 13.00±0.18Ac 8.88±0.12Dd 12.01±0.47Ac 10.16±0.22Dc
Citric Acid 14.24±0.76Aab 14.24±0.76Aab 14.90±0.85Aa 9.58±0.22Bb 14.45±0.65Aa 9.28±0.35Bb 13.15±0.79Abc 9.60±0.36Bb 12.96±0.18Acd 7.76±0.12Bc 11.90±0.45Ad 8.91±0.22Bb

a-dLetters indicate significant differences in the treatment, P < 0.05.

A-DLetters indicate significant differences among the different treatments, P < 0.05.

In contrast, glucose and galactose concentration started at a very low value (0.16± 0.007 mg.mL-1 and 0.89±0.044 mg.mL-1, respectively) and increased during the first hour. Then, these compounds decreased slowly and slightly in both treatments. At the end of the process, its glucose and galactose concentrations were P<0.05. LIT ended the process with glucose and galactose concentration equal to 26.010 ± 1.13 mg.mL-1 and 21.72 ± 0.75 mg.mL-1, respectively. Likewise, HIT obtained concentrations of 21.72 ± 0.75 mg.mL-1 of glucose and 14.74 ± 0.25 mg.mL-1 of galactose.

The lactic acid concentration at the beginning of fermentation was 0.152 ± 0.015 mg.mL-1 and after 300 minutes of procedure, the lactic acid concentration reached 18.644 ± 0.62mg.mL-1 in LIT and 17.557 ± 0.53 mg.mL-1 in HIT. From the beginning of procedure to the end, there was a significant difference between the treatments. However, either treatments have had an increase in lactic acid content. Regarding the acetic and citric acids content during fermentation, its amount remained constant around 13.52 ± 2.958 mg.mL-1 and 14.24 ± 0.757 mg.mL-1, respectively.

Discussion

Raw milk was analyzed and its result indicated good quality as expected. During fermentation, the initial pH of milk decreased, while titratable acidity increased in both treatments (LIT and HIT). These decrease and increase were evidently due the lactic acid production by lactose fermentation, as a result of microorganism metabolism. One of the lactic acid bacteria features are their capacity to ferment the mainly carbohydrate in milk and dairy products producing lactic acid as end product. The organic acids produced contribute not only to the flavor and aroma of fermented dairy products but also to their preservation.

Although a lower level of lactose results in a higher level of glucose and galactose, as it is seen in traditional yogurts(20), the addition of the enzyme showed a significantly lower lactose content, however, galactose and glucose values were not very high. The lactose hydrolysis by enzymatic via is one of the most evaluated strategies to achieve yogurt with low lactose content(14). The addition of β-galactosidase changes the carbohydrate pattern, which may affect the sugar consumption by the starter culture. Consequently, this fact could interfere with the production of derived compounds as well as the physicochemical and sensory characteristics of the products(11,15). Furthermore, lactic acid culture cleaved the lactose into its monosaccharides and organic acids component, being lactic acid the predominant one, as previously reported(3,16-19).

According to Mora et al.(21) and De Vin et al.(22), most of the lactic acid bacteria are efficiently capable of using the glucose portion of lactose and releasing galactose into the medium. S. thermophilus is able to metabolize lactose. In addition, the majority of this strains are galactose negative (Gal-). However, some strains are galactose positive (Gal+), i.e., they are able to metabolize galactose(9). This fact may explain the galactose behavior after 60 minutes fermentation.

In preheat treatment, the greater hydrolysis of lactose, observed at the beginning of fermentation, as compared to LIT treatment, is related to the increase in β-galactosidase activity. Whilst β -galactosidases are stable at temperatures between 20 and 37 °C, these enzymes are found to have optimum temperatures around 40 and 50 °C. In this condition, they retained 90-100% of their activity(23). As reported by Chandan and O’Rell(24), 40-45 °C is an optimum growth temperature for yogurt culture. It contributes to HIT quickly decreased pH and ripping. For the Maxilact® manufacturer description, the enzyme used to hydrolyze the lactose is a compound extracted from a milk yeast; therefore, the optimum conditions for their activity are similar to the natural milk pH (6.6) and temperature (35-40 °C).

Conclusion

Our findings confirmed that both treatments obtained a final low lactose concentration although there was P>0.05 between the different initial temperatures (30 or 41 °C. Thus, the enzyme contributed to reduce lactose to a low-level providing for consumption by humans who have lactose malabsorption. The simultaneous enzymatic lactose hydrolysis and lactose-cleaving activity by yogurt cultures did not prevent the fermentation process, regardless of the initial temperature, including proper yogurt fermentation and maintaining the physical and chemical characteristics.

Acknowledgments

We express our thanks to the “Coordenação de Aperfeiçoamento de Pessoal de Nível Superior” (CAPES) and to the “Pró Reitoria de Pesquisa, Pós Graduação e Inovação” of Fluminense Federal University (PROPPI-UFF) for the financial support for this research, in addition to the Veterinary Medicine Graduate Program.

References

1 Park YW, Juárez M, Ramos M, Haenlein GFW. Physico-chemical characteristics of goat and sheep milk. Small Ruminant Research; 2007. 68:88-113. [ Links ]

2 Fox PF. Lactose: Chemistry and Properties. In: McSweeney PLH, Fox PF. Advanced Dairy chemistry: Lactose, Water, Salts and Minor Constituents. 3rd rev. Ireland: Springer; 2009 . p. 1-13. [ Links ]

3 Tamine AY, Robinson RK. Yoghurt: science and technology. 3rd rev. New York: CRC Press; 2007. 791 p. [ Links ]

4 Misselwitz B, Pohl D, Frühauf H, Fried Michael, Vavricka SR and Fox M. Lactose malabsorption and intolerance: pathogenesis, diagnosis and treatment. United Gastroenterology Journal. 2013; 1(3):151-159. [ Links ]

5 Vuorisalo T, Arjamaa O, Vasemägi A, Taavitsainen J, Tourunen A, Saloniemi I. Perspectives in Biology an High Lactose Tolerance in North Europeans: A Result of Migration, Not In Situ Milk Consumption. Perspectives in Biology and Medicine. 2012; 55 (2): 163-174. [ Links ]

6 Raithel M, Weidenhiller M, Hagel AFK, Hetterich U, Neurath MF, Konturek PC. The Malabsorption of Commonly Occurring Mono and Disaccharides. Deutsches Ärzteblatt International. 2013; 110(46): 775-82. [ Links ]

7 Codex (2003) Codex Alimentarius: Milk and Milk Products - Standard for Fermented http://www.codexalimentarius.org/standards/list-of-standards/Milks. Available in: http://www.codexalimentarius.org/standards/list-of-standards. Accessed 01 sep 2016 [ Links ]

8 Adhikari K, Gruw IU, Mustapha A, Fernando LN. Changes in the Profile of Organic Acids in Plain Set and Stirred Yogurts During Manufacture and Refrigerated Storage. Journal of Food Quality. 2002; 25:435-451. [ Links ]

9 Anbukkarasi K, UmaMaheswari T, Hemalatha T, Nanda DK, Singh P, Singh R. Preparation of low galactose yogurt using cultures of Gal+ Streptococcus thermophilus in combination with Lactobacillus delbrueckii ssp. bulgaricus. Journal of Food Science and Technology. 2014; 51(9):2183-2189. [ Links ]

10 Schaafsma G. Lactose and lactase derivatives as bioactive ingredients in human nutrition. International Dairy Journal. 2008; 18:458-465. [ Links ]

11 Wolf IV, Vénica CI, Perotti M. Effect of reduction of lactose in yogurts by addition of b-galactosidase enzyme on volatile compound profile and quality parameters. Journal of Food Science and Technology. 2015; Doi:10.1111/ijfs.12745. [ Links ]

12 AOAC. Association Of Official Agricultural Chemists. Official methods of analysis. 19th rev. Gaithersburg , MD -USA: AOAC Intl. 2012. [ Links ]

13 González de Llano D; Rodriguez A; Cuesta P. Effect of lactic starter cultures on the organic acid composition of milk and cheese during ripening - Analysis by HPLC. Journal of Applied Microbiology. 1996; 80:570-576 [ Links ]

14 Mlichova Z, Rosenberg M. Current trends of b-galactosidase application in food technology. Journal of Food and Nutrition Research. 2006; 45:47-54. [ Links ]

15 Vénica CI, Bergamini CV, Zalazar CA,Perotti MC. Effect of lactose hydrolysis during manufacture and storage of drinkable yogurt. Journal of Food & Nutritional Disorders. 2013; 2:5 [ Links ]

16 O'leary VS, Woychik JH. Utilization of lactose, glucose, and galactose by a mixed culture of Streptococcus thermophilus and Lactobacillus bulgaricus in milk treated with lactase enzyme. Applied and Environmental Microbiology. 1976; 32: 89-94 [ Links ]

17 Dave RI, Shah NP. Viability of yoghurt and probiotic bacteria in yoghurts made from commercial starter cultures. International Dairy Journal. 1997; 7(1):31-41 [ Links ]

18 Ordóñez JA., Rodriguez MIC, Álvarez LF, Sanz MLG, Minguillón FDGF, Parales LH, Cortecero MDS. Tecnologia de alimentos - Alimentos de Origem Animal v.2. Porto Alegre: Artmed; 2005. 280p. [ Links ]

19 Walstra P, Wouters JTM, Geurts TJ. Dairy Science and Technology. 2nd rev. London: CRC Press; 2014. 808 p. [ Links ]

20 Costa MP, Frasao BS, Lima BRCC, Rodrigues BL, Conte Junior CA. Simultaneous analysis of carbohydrates and organic acids by HPLC- DAD-RI for monitoring goat's milk yogurts fermentation. Talanta. 2016; 162-170. [ Links ]

21 Mora D, Fortina MG, Parini C, Ricci G, Gatti M, Giraffa G, Manachini PL. Genetic diversity and technological properties of Streptococcus thermophilus strains isolated from dairy products. Journal of Applied Microbiology. 2002; 93(2):278-287. [ Links ]

22 De Vin F, Radström P, Herman L, De Vuyst L. Molecular and biochemical analysis of the galactose phenotype of dairy Streptococcus thermophilus strains reveals four different fermentation profiles. Applied and Environmental Microbiology. 2005; 7:3659-3667. [ Links ]

23 Ustok FI, Tari C, Harsa S. Biochemical and thermal properties of ß-galactosidase enzymes produced by artisanal yoghurt cultures. Food Chemistry. 2010; 119:1114-1120. [ Links ]

24 Chandan RC, O'Rell K. Yogurt: fruit preparations and fl avoring materials In: Chandan RC, Kilara A. Manufacturing Yogurt and Fermented Milks. 2nd rev. West Sussex: Wiley Blackwell; 2013. p. 193-215. [ Links ]

Received: October 04, 2016; Accepted: July 11, 2018

*Corresponding author - raphaelfbarros@veterinario.med.br

Creative Commons License This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.