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Artigos • Ciênc. Florest. 23 (4) • Oct-Dec 2013 • https://doi.org/10.5902/1980509812343   copy

SOMATIC EMBRYOGENESIS AND MORPHOANATOMY OF Ocotea porosa SOMATIC EMBRYOS

EMBRIOGÊNESE SOMÁTICA E MORFOANATOMIA DE EMBRIÕES SOMÁTICOS DE Ocotea porosa

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Ocotea porosa seeds have strong tegument dormancy, recalcitrant behavior, low and irregular germination and that makes its natural propagation difficult. The aim of this study was to establish a protocol of regeneration of Ocotea porosa from somatic embryogenesis. Immature embryonic axes were inoculated on WPM culture medium supplemented with 2.4-D (200 µM) combined or not with hydrolyzed casein or glutamine (0.5 or 1 g l-1), during 90 days. The repetitive embryogenesis was induced on medium with 2.4-D (22.62 µM) combined with 2-iP (2.46 µM) followed by transfer to culture medium with hydrolyzed casein or glutamine (1 g l-1) during 90 days. The maturation of somatic embryos was tested in culture medium containing NAA (0.5 μM) and 2-iP (5; 10 and 20 μM). The highest percentage of somatic embryos induction (8.3%) was observed in WPM culture medium containing 200 µM 2.4-D and 1 g L-1 hydrolyzed casein and the development of somatic embryos occurred indirectly. Repetitive somatic embryogenesis was promoted in WPM medium containing hydrolyzed casein or glutamine. However, the culture medium containing hydrolyzed casein promoted the maintenance of embryogenic capacity for more than two years. During the maturity phase, there was a low progression of globular embryos to cordiform and torpedo stages. The different ontogenetic stages of somatic embryos of Ocotea porosa were characterized by histological studies.

Keywords:
Embryogenic mass; hydrolyzed casein; glutamine; histology

RESUMO

Ocotea porosa apresenta sementes com forte dormência tegumentar, comportamento recalcitrante, baixa germinação e irregularidade, o que dificulta a propagação natural. O objetivo deste trabalho foi estabelecer um protocolo de embriogênese somática para Ocotea porosa. Eixos embrionários zigóticos imaturos foram inoculados em meio de cultura WPM suplementado com 2,4-D (200 µM) combinado ou não com caseína hidrolisada ou glutamina (0,5 ou 1 g l-1) durante 90 dias. A embriogênese repetitiva foi induzida em meio contendo 2,4-D (22,62 µM) combinado com 2-iP (2,46 µM) seguido de transferência para meio de cultura com caseína hidrolisada ou glutamina (1 g l-1), durante 90 dias cada. A maturação dos embriões somáticos foi testada em meio de cultura contendo ANA (0,5 μM) e 2-iP (5, 10 ou 20 μM). A maior porcentagem de embriões somáticos induzidos (8,3%) foi observada em meio de cultura WPM contendo 200 µM de 2,4-D e 1 g l-1 de caseína hidrolisada e o desenvolvimento de embriões somáticos ocorreram de forma indireta. A embriogênese somática repetitiva foi promovida em meio de cultura WPM contendo caseína hidrolisada ou glutamina, no entanto, a caseína hidrolisada promoveu a manutenção da competência embriogênica por mais de dois anos. Na fase de maturação, houve baixa progressão dos embriões globulares para os estádios cordiforme e torpedo. Os diferentes estádios ontogenéticos de embriões somáticos de Ocotea porosa foram caracterizados pelo estudo histológico.

Palavras-chave:
massa embriogênica; caseína hidrolisada; glutamina; histologia

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REFERENCES

  • BENSON, E. E. In vitro plant recalcitrance: an introduction. In Vitro Cellular Developmental Biology - Plant, Wallinghord, v. 36, n3, p. 141-148, maio/jun. 2000.
  • CARVALHO, P. E. R. Espécies arbóreas brasileiras. Brasília: Embrapa Informação Tecnológica; Colombo, PR: Embrapa Florestas, 2003, 1039p.
  • CARVALHO, D. C. et al. Embriogênese somática do caquizeiro. Revista Brasileira de Fruticultura, Jaboticabal, v. 26, n. 2, p. 280-283, ago. 2004.
  • CANHOTO, J. M. et al. Somatic embryogenesis in Bay Laurel (Laurus nobilis). In: JAIN, S. M.; GUPTA, P. K.; NEWTON, R. J. Somatic embryogenesis in woody plants. Dordrecht: Kluwer Academic Publishers, 1999. v. 4, p. 341-367.
  • CANHOTO, J. M. et al. Protocol for somatic embryogenesis: tamarillo (Cyphomandra betacea (Cav.) Sendt.). In: JAIN, S. M.; GUPTA, P. K. (eds.). Protocol for Somatic embryogenesis in woody plants . Dordrecht: Springer, 2005, p 379-389.
  • DODEMAN, V. L. et al. Zygotic embryogenesis versus somatic embryogenesis. Journal of Experimental Botany, v. 48, p. 1493-1509, 1997.
  • GARCIA-MARTIN, G. et al. Effects of exogenous ABA on embryo maturation and quantification of endogenous levels of ABA and IAA in Quercus suber somatic embryos. Plant Cell, Tissue and Organ Culture, v. 80, p. 171-177, 2005.
  • GARIN, E. et al. Effect of sugars, amino acids, and culture technique on maturation of somatic embryos of Pinus strobus on medium with two gellan gum concentration. Plant Cell, Tissue and Organ Culture , Dordrecht, v. 62, n. 1, p. 27-37, jul. 2000.
  • GEORGE, E. F. Plant propagation by tissue culture. 2nd ed. London: Exegetics Limited, 1996. v. 2.
  • GEORGE, E. F.; DE KLERK, G. J. The Components of Plant Tissue Culture Media I: Macro and Micro-Nutrients. In: GEORGE, E.F.; HALL, M.A.; DE KLERK, G.J. Plant propagation by tissue culture . The Background, 2008. v. 1
  • GUERRA, P. M. et al. Embriogênese somática e semente sintética. In: TORRES, A.; CALDAS L. S.; BUSO, J. A. Técnicas e aplicações da cultura de tecidos de plantas. Brasília: ABCTP/EMBRAPA - CNPH, p. 533-568, 1999.
  • GUERRA, M. P.; HANDRO, W. Somatic embryogenesis and plant regeneration in different organs of Euterpe edulis Mart. (Palmae): control and structural features. Journal of Plant Research, Tokyo, v. 111, n. 1, p. 65-71, mar. 1998.
  • HERNÁNDEZ, I. et al. Vegetative propagation of Quercus suber L. by somatic embryogenesis: II. Plant regeneration from selected cork oak trees. Plant Cell Reports, Berlin, v. 21, n.8, p. 765-770, abr. 2003.
  • IBAMA - Instituto Brasileiro do Meio Ambiente. Portaria n. 37-N, Lista oficial de espécies da flora brasileira ameaçadas de extinção, 3 de abril de 1992.
  • INOUE, M. T.; PUTTON, V. Macropropagação de 12 espécies arbóreas da floresta ombrófila mista. Floresta, Curitiba, v. 37, n. 1, p. 55-61, jan./abr. 2007.
  • JIMÉNEZ, V. M. Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regulator, v. 47, p. 91-110, 2005.
  • KARNOVSKY, M. J. A. Formaldehyde- glutaraldehyde fixative of high osmolality for use in eletron microscopy. Journal of Cell Biology, New Yorkv. 27, n. 10, p. 137-138, 1965.
  • KOMAMINE, A. et al. Mechanisms of somatic embryogenesis in carrot suspension cultures - morphology, physiology, biochemistry, and molecular biology. In Vitro Cellular Developmental Biology - Plant , Wallinghord, v. 41, n. 1, p. 6-10, Jan./Feb. 2005.
  • LEA, P. J. Nitrogen metabolism. In: LEA, P. J.; LEEGOOD, R. C. (eds). Plant Biochemistry and Molecular Biology. New York: Wiley & Sons, 1993. p 155-180.
  • LITZ, R. E. et al. Somatic embryogenesis in mango (Mangifera indica L.). In: JAIN, S. M.; GUPTA, P. K.; NEWTON, R. J. (ed.). Somatic embryogenesis in woody plants, Angiosperms. Dordrecht: Kluwer Academic Publishers , 1995. v. 2, p. 341-357.
  • LLOYD, G.; MCCOWN, B. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Proceedings of International Plant Propagation Society, Ashville, v. 30, p. 421-427, 1980.
  • MARTIN, K. P.; MADASSERY, J. Direct and indirect somatic embryogenesis on cotyledon explants of Quassia amara L., an antileukaemia drug plant. In Vitro Cellular Developmental Biology - Plant , Wallinghord, v. 41, n. 1, p. 54-57, Jan./Feb. 2005.
  • MARTINELLI, L. et al. Morphogenic competence of Vitis rupestris S. secondary somatic embryos with a long culture history. Plant Cell Reports , Berlin, v. 20, n.4, p. 279-284, June 2001.
  • MERKLE, S. A. et al. Morphogenic aspects of somatic embryogenesis. In: THORPE, T. A. In vitro embryogenesis in plants. Dordrecht: Kluwer Academic, 1995. p. 155-203.
  • MORCILLO, F. et al. Differential effects of glutamine and arginine on 7S globulin accumulation during the maturation of Oil Palm somatic embryos. Plant Cell Reports , Berlin, v. 18, n. 10, p. 868-872, June/July 1999.
  • MOTOIKE, S. Y. et al. Somatic embryogenesis of Myrciaria aureana (Brazilian grape tree). Plant Cell, Tissue and Organ Culture , v. 89, p. 75 - 81, 2007.
  • MOURA-COSTA, P. H. et al. In vitro plantlet regeneration of Ocotea catharinensis, an endangered Brazilian hardwood forest tree. Plant Cell, Tissue and Organ Culture , Dordrecht, v. 35, p. 279-286, 1993.
  • O'BRIEN, T. P. et al. Polychromatic Staining of Plant Cell Walls by Toluidine Blue O. Protoplasma, v. 59, n. 2, p. 368-373, 1965.
  • ORTIZ-LOPEZ, A. et al. Amino acid transporters in plants. Biochimica et Biophysica Actav. 1465, n. 1-2, p. 275-280, maio 2000.
  • PERÁN-QUESADA, R. et al. Factors affecting maturation of avocado somatic embryos. Scientia Horticulturae., v. 102, p. 61-73, 2004.
  • PEREZ-NUÑEZ, M. T. et al. Improved somatic embryogenesis from Cocos nucifera (L) plumule explants. In Vitro Cellular Development Biology - PlantWallinghord, v. 42, n.1, p. 37-43, Jan./Feb. 2006.
  • PINTOS, B. et al. Synthetic seed production from encapsulated somatic embryos of cork oak (Quercus suber L.) and automated growth monitoring. Plant Cell, Tissue and Organ Culture , v. 95, n.2, p. 217-225, Nov. 2008.
  • PINTO, G. et al. Somatic embryogenesis in leaf callus from a mature Quercus suber L. tree. In Vitro Cellular Development Biology - Plant , Wallinghord, v. 38, n.6, p. 569-572, Nov./Dec. 2002.
  • QUEIROZ-FIGUEROA, F. R. et al. Embryo production through somatic embryogenesis can be used to study cell differentiation in plants. Plant Cell, Tissue and Organ Culture , v. 86, n.3, p.285-301, Sept. 2006.
  • RAI, M.K. et al. The role of abscisic acid in plant tissue culture: a review of recent progress. Plant Cell, Tissue Organ and Culture, v. 106, p. 79 -190, 2011.
  • RIBAS, L. L. F. et al. Somatic embryogenesis in Aspidosperma polyneuron Mull. Arg. In: JAIN, S. M.; GUPTA, P. K.; NEWTON, R. J. (ed.). Somatic embryogenesis in woody plants . Dordrecht: Springer , 2000. v. 6, p. 509-537, 2000.
  • SANCHEZ-ROMERO, C. et al. Somatic and zygotic embryogenesis in Avocado. In: MEYIB, A.; SAMAY, J. (ed.) Somatic embryogenesis. Plant Cell Monographsv. 2, p. 271-284, 2006.
  • SANTA-CATARINA, C. et al. Germinação in vitro e embriogênese somática a partir de embriões imaturos de canela sassafrás (Ocotea odorifera Mez). Revista Brasileira de Botânica, São Paulo, v. 24, n. 4, p. 501-510, dez. 2001.
  • SANTA-CATARINA, C. et al. Protocol of somatic embryogenesis: Ocotea catharinensis Mez (Lauraceae). In: JAIN, S. M.; GUPTA, P. K. (ed.). Protocol for Somatic embryogenesis in woody plants . Dordrecht: Springer , 2005. p. 427-443.
  • SANTOS, A. L. et al. Somatic embryogenesis in parana pine (Araucaria angustifolia (Bert.) O. Kuntze). Brazilian Archives of Biology and Technology, v. 45, n. 1, p. 97-106, Mar. 2002.
  • SEMA - Secretaria de Estado do Meio Ambiente. Lista vermelha das plantas ameaçadas de extinção no Estado do Paraná. Curitiba: SEMA: GTZ, 1995.
  • SHI, X. et al. Enhancement of somatic embryogenesis in camphor tree (Cinnamomum camphora L.): osmotic stress and other factors affecting somatic embryo formation on hormone-free medium. Trees, v. 23, p. 1033-1042, 2009.
  • STATSOFT, INC. Statistica (data analysis software system), version 7.1. <http://www.statsoft.com>. 2005.
    » http://www.statsoft.com
  • TITON, M. et al. Efeito dos reguladores de crescimento dicamba e picloram na embriogênese somática em Eucalyptus grandis Revista Árvore, Viçosa, v. 31, n. 003, p. 417-426, maio./jun. 2007.
  • TULECKE, W. et al. Somatic embryogenesis in walnut (Juglans species). In: BAJAJ, Y. P. S. (ed.). Biotechnology in agriculture and forestry, somatic embryogenesis and synthetic seed. Springer-Verlag, 1995. v. 30, p. 370-377.
  • VAHDATI, K. et al. Effect of exogenous ABA on somatic embryo maturation and germination in Persian walnut (Juglans regia L.). Plant Cell, Tissue and Organ Culture , v. 93, p. 163-171, 2008.
  • VIANA, A. M.; MANTELL, S. H. Somatic embryogenesis of Ocotea catharinensis, an endangered tree of the mata atlântica (South Brazil). In: JAIN, S. M.; GUPTA, P. K.; NEWTON, R. J. (ed). Somatic embryogenesis in woody plants . Dordrecht: Kluwer Academic Publishers , 1999. v. 5, p. 197-214.

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