Effect of chlorhexidine concentration on the bond strength to dentin in primary teeth

Manfro, Aline Rosler Grings; Reis, Alessandra; Loguercio, Alessandro Dourado; Imparato, José Carlos Pettorossi; Raggio, Daniela Prócida

doi:10.1590/S1980-65232010000100018

Abstracts

PURPOSE: To evaluate the effect of the use of 0.5% and 2% chlorhexidine digluconate on the immediate bond strength of a conventional adhesive system to dentin in primary teeth. METHODS: Twenty-one healthy primary molars were divided into three groups (n=7), being one control (A) and two experimental groups (B and C). After dentin exposure, in Group (A) the adhesive procedure was performed using 37% phosphoric acid gel (15 s); dentin was washed (15 s), air dried (30 s) and rehydrated with water. Groups B and C followed similar procedures but for re-hydration with 0.5% and 2% chlorhexidine, respectively, for 30 s. A resin composite block was built simulating a restoration, and the teeth were stored in distilled water at 37°C for 24 h before the microtensile bond strength test. The bond strength data were analyzed by analysis of variance. RESULTS: No statistically significant difference in bond strength was found among the tested groups (P>0.05) CONCLUSION: The 0.5% and 2% concentrations of chlorhexidine presented similar behavior and caused no adverse effects on the bond strength to dentin in primary teeth.

Chlorhexidine; bond strength; microtensile; primary teeth

OBJETIVO: Avaliar o efeito do uso de digluconato de clorexidina 0,5% e 2% na resistência de união imediata à dentina de dentes decíduos para um sistema adesivo convencional. METODOLOGIA: Vinte e um molares decíduos hígidos foram divididos em três grupos (n=7), sendo um controle e dois experimentais. Após a exposição da dentina, foi realizado no grupo controle (A) o procedimento adesivo utilizando ácido fosfórico gel a 37% (15 s); a superfície foi então lavada (15 s), seca com ar (30 s) e reidratada com água. Os grupos B e C foram idênticos ao grupo A, apenas com diferença no reumidecimento de clorexidina 0,5% e 2% respectivamente, por 30 s. Após a confecção do bloco de resina composta, os dentes foram armazenados em água destilada a 37°C por 24 h antes do teste de microtração. Os dados de resistência de união foram avaliados através de análise de variância. RESULTADOS: Os dados apresentaram distribuição homogênea, não havendo diferença estatisticamente significante entre os grupos (P>0,05). CONCLUSÃO: As concentrações de clorexidina a 0,5% e 2% apresentaram comportamentos similares e não causaram efeitos adversos na resistência de união em dentina de dentes decíduos, quando comparadas ao grupo controle.

Clorexidina; resistência de união; microtração; dentes decíduos

ORIGINAL ARTICLE

Effect of chlorhexidine concentration on the bond strength to dentin in primary teeth

Efeito da concentração de clorexidina na resistência de união à dentina em dentes decíduos

Aline Rosler Grings Manfro^I; Alessandra Reis^II; Alessandro Dourado Loguercio^II; José Carlos Pettorossi Imparato^III; Daniela Prócida Raggio^IV

^IPost Graduation Course in Pediatric Dentistry, Dental Research Center São Leopoldo Mandic, Campinas, SP, Brazil

^IISchool of Dentistry, State University of Ponta Grossa, Ponta Grossa, PR, Brazil

^IIIPost Graduation Program, Dental Research Center São Leopoldo Mandic, Campinas, SP, Brazil

^IVDiscipline of Pediatric Dentistry, University of São Paulo, São Paulo, SP, Brazil

^{Correspondence} Correspondence: Daniela Prócida Raggio Av. Lineu Prestes, 2227 Cidade Universitária São Paulo, SP - Brasil 05508-000 E-mail: danielar@usp.br

ABSTRACT

PURPOSE: To evaluate the effect of the use of 0.5% and 2% chlorhexidine digluconate on the immediate bond strength of a conventional adhesive system to dentin in primary teeth.

METHODS: Twenty-one healthy primary molars were divided into three groups (n=7), being one control (A) and two experimental groups (B and C). After dentin exposure, in Group (A) the adhesive procedure was performed using 37% phosphoric acid gel (15 s); dentin was washed (15 s), air dried (30 s) and rehydrated with water. Groups B and C followed similar procedures but for re-hydration with 0.5% and 2% chlorhexidine, respectively, for 30 s. A resin composite block was built simulating a restoration, and the teeth were stored in distilled water at 37°C for 24 h before the microtensile bond strength test. The bond strength data were analyzed by analysis of variance.

RESULTS: No statistically significant difference in bond strength was found among the tested groups (P>0.05)

CONCLUSION: The 0.5% and 2% concentrations of chlorhexidine presented similar behavior and caused no adverse effects on the bond strength to dentin in primary teeth.

Key words: Chlorhexidine; bond strength; microtensile; primary teeth

RESUMO

OBJETIVO: Avaliar o efeito do uso de digluconato de clorexidina 0,5% e 2% na resistência de união imediata à dentina de dentes decíduos para um sistema adesivo convencional.

METODOLOGIA: Vinte e um molares decíduos hígidos foram divididos em três grupos (n=7), sendo um controle e dois experimentais. Após a exposição da dentina, foi realizado no grupo controle (A) o procedimento adesivo utilizando ácido fosfórico gel a 37% (15 s); a superfície foi então lavada (15 s), seca com ar (30 s) e reidratada com água. Os grupos B e C foram idênticos ao grupo A, apenas com diferença no reumidecimento de clorexidina 0,5% e 2% respectivamente, por 30 s. Após a confecção do bloco de resina composta, os dentes foram armazenados em água destilada a 37°C por 24 h antes do teste de microtração. Os dados de resistência de união foram avaliados através de análise de variância.

RESULTADOS: Os dados apresentaram distribuição homogênea, não havendo diferença estatisticamente significante entre os grupos (P>0,05).

CONCLUSÃO: As concentrações de clorexidina a 0,5% e 2% apresentaram comportamentos similares e não causaram efeitos adversos na resistência de união em dentina de dentes decíduos, quando comparadas ao grupo controle.

Palavras-chave: Clorexidina; resistência de união; microtração; dentes decíduos

Texto completo disponível apenas em PDF.

Full text available only in PDF format.

Received: October 1, 2009

Accepted: December 21, 2009

1. Pashley DH, Carvalho RM. Dentine permeability and dentine adhesion. J Dent 1997;25:355-72.
2. Wang Y, Spencer P. Quantifying adhesive penetration in adhesive/dentin interface using confocal Raman microspectroscopy. J Biomed Mater Res 2002;59:46-55.
3. Ferracane JL. Hygroscopic and hydrolytic effects in dental polymer networks. Dent Mater 2006;22:211-22.
4. Hashimoto M, Ohno H, Sano H, Tay FR, Kaga M, Kudou Y, et al. Micromorphological changes in resin-dentin bonds after 1 year of water storage. J Biomed Mater Res 2002;63:306-11.
5. Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, et al. Collagen degradation by host-derived enzymes during aging. J Dent Res 2004;83:216-21.
6. Modesto A, Drake DR. Multiple exposures to chlorhexidine and xylitol: adhesive and biofilm formulation by Streptococcus mutans Curr Microbiol 2006;52:418-23.
7. Sulkala M, Tervahartiala T, Sorsa T, Larmas M, Salo T, Tjärdehane L. Matrix metalloproteinase-8 (MMP-8) is the major collagenase in human dentin. Arch Oral Biol 2007;52:121-7.
8. Hashimoto M, Tay FR, Ohno H, Kaga M, Yiu C, Kumagai H, et al. SEM and TEM analysis of water degradation of human dentinal collagen. J Biomed Mater Res B Appl Biomater 2003;66:287-98.
9. Hebling J, Pashley DH, Tjäderhane L, Tay FR. Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res 2005; 84:741-6.
10. Carrilho MR, Carvalho RM, de Goes MF, di Hipolito V, Geraldeli S, Tay FR, et al. Chlorhexidine preserves dentin bond in vitro. J Dent Res 2007;86:90-4.
11. Fukae M, Kaneko I, Tanabe T, Shimizu M. Metalloproteinases in the mineralized compartments of porcine dentine as detected by substrate-gel electrophoresis. Arch Oral Biol 1991;36:567-73.
12. Carrilho MR, Tay FR, Donnelly AM, Agee KA, Tjäderhane L, Mazzoni A, et al. Host-derived loss of dentin matrix stiffness with solubilization of collagen. J Biomed Mater Res B Appl Biomater 2009;90:373-80.
13. Stanislawczuk R, Amaral RC, Grande CZ, Gagler D, Reis A, Loguercio AD. Chlorhexidine-containing acid conditioner preserves the longevity of resin-dentin bonds. Oper Dent 2009;34:483-92.
14. Reis A, Loguercio AD, Azevedo CLN, Carvalho RM, Singer JM, Grande RHM. Moisture spectrum of demineralized dentin for adhesive systems with different solvent-based. J Adhes Dent 2003;5:183-92.
15. Ercan E, Ozekinci T, Atakul F, Gül K. Antibacterial activity of 2% chlorhexidine gluconate and 5,25% sodium hypochlorite in infected root canal: in vivo study. J Endod 2004;30:84-7.
16. Perdigão J, Denehy GE, Swift EJ Jr. Effects of chlorhexidine on dentin surfaces and shear bond strengths. Am J Dent 1994;7:81-4.
17. Stanley A, Wilson M, Newman HN. The in vitro effects of chlorhexidine on subgingival plaque bacteria. J Clin Periodontol 1989;16:259-64.
18. Greenstein G, Berman C, Jaffin R. Chlorhexidine. An adjunct to periodontal therapy. J Periodontol 1986;57:370-7.
19. Vieira RS, da Silva IA. Bond strength to primary tooth dentin following disinfection with a chlorhexidine solution: an in vitro study. Pediatr Dent 2003;25:49-52.
20. Ersin NK, Candan U, Aykut A, Eronat C, Belli S. No adverse effect to bonding following caries disinfection with chlorhexidine. J Dent Child 2009;76:20-7.
21. Soares CJ, Pereira CA, Pereira JC, Santana FR, Prado CJ. Effect of chlorhexidine application on microtensile bond strength to dentin. Oper Dent 2008;33:183-8.
22. Brackett WW, Tay FR, Brackett MG, Dib A, Sword RJ, Pashley DH. The effect of chlorhexidine on dentin hybrid layers in vivo. Oper Dent 2007; 32:107-11.
23. Breschi L, Cammelli F, Visintini E, Mazzoni A, Vita F, Carrilho M, et al. Influence of chlorhexidine concentration on the durability of etch-and-rinse dentin bonds: a 12-mounth in vitro study. J Adhes Dent 2009;11:191-8.

Correspondence:

Daniela Prócida Raggio

Av. Lineu Prestes, 2227 Cidade Universitária

São Paulo, SP - Brasil 05508-000

E-mail:

danielar@usp.br

Publication Dates

Publication in this collection
26 Aug 2011
Date of issue
2010

History

Received
01 Oct 2009
Accepted
21 Dec 2009

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1. Pashley DH, Carvalho RM. Dentine permeability and dentine adhesion. J Dent 1997;25:355-72.

[2] 2. Wang Y, Spencer P. Quantifying adhesive penetration in adhesive/dentin interface using confocal Raman microspectroscopy. J Biomed Mater Res 2002;59:46-55.

[3] 3. Ferracane JL. Hygroscopic and hydrolytic effects in dental polymer networks. Dent Mater 2006;22:211-22.

[4] 4. Hashimoto M, Ohno H, Sano H, Tay FR, Kaga M, Kudou Y, et al. Micromorphological changes in resin-dentin bonds after 1 year of water storage. J Biomed Mater Res 2002;63:306-11.

[5] 5. Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM, et al. Collagen degradation by host-derived enzymes during aging. J Dent Res 2004;83:216-21.

[6] 6. Modesto A, Drake DR. Multiple exposures to chlorhexidine and xylitol: adhesive and biofilm formulation by Streptococcus mutans Curr Microbiol 2006;52:418-23.

[7] 7. Sulkala M, Tervahartiala T, Sorsa T, Larmas M, Salo T, Tjärdehane L. Matrix metalloproteinase-8 (MMP-8) is the major collagenase in human dentin. Arch Oral Biol 2007;52:121-7.

[8] 8. Hashimoto M, Tay FR, Ohno H, Kaga M, Yiu C, Kumagai H, et al. SEM and TEM analysis of water degradation of human dentinal collagen. J Biomed Mater Res B Appl Biomater 2003;66:287-98.

[9] 9. Hebling J, Pashley DH, Tjäderhane L, Tay FR. Chlorhexidine arrests subclinical degradation of dentin hybrid layers in vivo. J Dent Res 2005; 84:741-6.

[10] 10. Carrilho MR, Carvalho RM, de Goes MF, di Hipolito V, Geraldeli S, Tay FR, et al. Chlorhexidine preserves dentin bond in vitro. J Dent Res 2007;86:90-4.

[11] 11. Fukae M, Kaneko I, Tanabe T, Shimizu M. Metalloproteinases in the mineralized compartments of porcine dentine as detected by substrate-gel electrophoresis. Arch Oral Biol 1991;36:567-73.

[12] 12. Carrilho MR, Tay FR, Donnelly AM, Agee KA, Tjäderhane L, Mazzoni A, et al. Host-derived loss of dentin matrix stiffness with solubilization of collagen. J Biomed Mater Res B Appl Biomater 2009;90:373-80.

[13] 13. Stanislawczuk R, Amaral RC, Grande CZ, Gagler D, Reis A, Loguercio AD. Chlorhexidine-containing acid conditioner preserves the longevity of resin-dentin bonds. Oper Dent 2009;34:483-92.

[14] 14. Reis A, Loguercio AD, Azevedo CLN, Carvalho RM, Singer JM, Grande RHM. Moisture spectrum of demineralized dentin for adhesive systems with different solvent-based. J Adhes Dent 2003;5:183-92.

[15] 15. Ercan E, Ozekinci T, Atakul F, Gül K. Antibacterial activity of 2% chlorhexidine gluconate and 5,25% sodium hypochlorite in infected root canal: in vivo study. J Endod 2004;30:84-7.

[16] 16. Perdigão J, Denehy GE, Swift EJ Jr. Effects of chlorhexidine on dentin surfaces and shear bond strengths. Am J Dent 1994;7:81-4.

[17] 17. Stanley A, Wilson M, Newman HN. The in vitro effects of chlorhexidine on subgingival plaque bacteria. J Clin Periodontol 1989;16:259-64.

[18] 18. Greenstein G, Berman C, Jaffin R. Chlorhexidine. An adjunct to periodontal therapy. J Periodontol 1986;57:370-7.

[19] 19. Vieira RS, da Silva IA. Bond strength to primary tooth dentin following disinfection with a chlorhexidine solution: an in vitro study. Pediatr Dent 2003;25:49-52.

[20] 20. Ersin NK, Candan U, Aykut A, Eronat C, Belli S. No adverse effect to bonding following caries disinfection with chlorhexidine. J Dent Child 2009;76:20-7.

[21] 21. Soares CJ, Pereira CA, Pereira JC, Santana FR, Prado CJ. Effect of chlorhexidine application on microtensile bond strength to dentin. Oper Dent 2008;33:183-8.

[22] 22. Brackett WW, Tay FR, Brackett MG, Dib A, Sword RJ, Pashley DH. The effect of chlorhexidine on dentin hybrid layers in vivo. Oper Dent 2007; 32:107-11.

[23] 23. Breschi L, Cammelli F, Visintini E, Mazzoni A, Vita F, Carrilho M, et al. Influence of chlorhexidine concentration on the durability of etch-and-rinse dentin bonds: a 12-mounth in vitro study. J Adhes Dent 2009;11:191-8.