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Articles • Rev. Ambient. Água 9 (4) • Dec 2014 • https://doi.org/10.4136/ambi-agua.1473   copy

Tissue levels of the antioxidant enzymes superoxide dismutase and catalase in fish Astyanax bimaculatus from the Una River Basin

Níveis teciduais das enzimas antioxidantes superóxido dismutase e catalase no peixe Astyanax bimaculatus da bacia hidrográfica do rio Una

Authorship SCIMAGO INSTITUTIONS RANKINGS

Abstracts

This paper seeks to identify the biomarker response to oxidative stress in Astyanax bimaculatus, a freshwater fish, collected from the Una River and its associated water bodies. The fish were collected using fishing nets at three different points on the river basin, namely Fazenda Piloto (FP), Ipiranga (IP) and Remédios (RM), during the period from December 2013 to March 2014. Physical and chemical analyses of the water at the sample locations indicate that IP and RM possibly have larger concentration of either natural or anthropic pollutants as compared to FP. FP can therefore be considered as the point less impacted by pollutants than other points. Hepatic activity of antioxidant stress enzymes, superoxide dismutase (SOD) and catalase (CAT), were measured in the specimens. The levels of SOD were reduced at RM while they were elevated in fish collected at IP. The CAT levels for the fish at RM and IP were about 148.9% and 202.4% above the values at FP, respectively. These results suggest that antioxidant enzymes could be used as biomarkers to measure oxidative stress caused by pollutants in the Una River Basin.

biomonitoring; pollution; radical oxygen species

O presente trabalho teve como objetivo identificar respostas de biomarcadores de estresse oxidativo no peixe de água doce Astyanax bimaculatus, coletado nos rios e corpos d'água da bacia hidrográfica do rio Una. Os peixes foram coletados com rede de pesca entre dezembro de 2013 e março de 2014, em três diferentes pontos da bacia: Fazenda Piloto (FP), Ipiranga (IP) e Remédios (RM). Análises físico-químicas das amostras de água nos pontos de coleta indicaram que IP e RM possivelmente apresentaram uma maior concentração de poluentes, naturais ou antrópicos, quando comparados ao FP. Portanto, FP pode ser considerado como o ponto menos impactado por poluentes em relação aos outros pontos. Níveis hepáticos de atividade das enzimas antioxidantes superóxido dismutase (SOD) e catalase (CAT) foram medidos nos espécimes. Os níveis de SOD foram reduzidos no RM, enquanto que eles foram aumentados em peixes coletados em IP. Os níveis de CAT nos peixes coletados em RM e IP foram 148,9% e 202,4% maiores do que os valores encontrados nos peixes do ponto FP, respectivamente. Os resultados sugerem que as enzimas antioxidantes podem ser usadas como biomarcadoras para mensurar os níveis de stress oxidativo causado pelos poluentes na bacia hidrográfica do rio Una.

biomonitoramento; espécies reativas de oxigênio; poluição

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Publication Dates

  • Publication in this collection
    31 Oct 2014
  • Date of issue
    Dec 2014

History

  • Received
    26 July 2014
  • Accepted
    29 Sept 2014
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Instituto de Pesquisas Ambientais em Bacias Hidrográficas Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHi), Estrada Mun. Dr. José Luis Cembranelli, 5000, Taubaté, SP, Brasil, CEP 12081-010 - Taubaté - SP - Brazil
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