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Brazilian Journal of Food Technology

On-line version ISSN 1981-6723

Braz. J. Food Technol. vol.24  Campinas  2021  Epub Apr 09, 2021

https://doi.org/10.1590/1981-6723.01720 

ORIGINAL ARTICLE

Continuous flow process to make fermented cassava starch substitute: I. Physicochemical and functional properties

Produção de sucedâneo de polvilho azedo em processo contínuo: I. Propriedades físico-químicas e funcionais

Vitor Brito1  * 
http://orcid.org/0000-0002-6304-6415

Renata Santos2 

Jeniffer Narcisa-Oliveira2 

Ivo Demiate3 

Marney Cereda2 
http://orcid.org/0000-0002-8181-7754

1Universidade para o Desenvolvimento do Estado e da Região do Pantanal (UNIDERP), Departamento de Produção e Gestão Agroindustrial, Campo Grande/MS - Brasil

2Universidade Católica Dom Bosco (UCDB), Departamento de Ciências Ambientais e Sustentabilidade Agropecuária, Campo Grande/MS - Brasil

3Universidade Estadual de Ponta Grossa (UEPG), Ponta Grossa/PR - Brasil


Abstract

The Brazilian fermented cassava starch (polvilho azedo) is prized as a raw material for fast food preparation. Its traditional production by natural fermentation and exposure to sunlight favors contamination and impurities, in addition to depending on the climate. The literature explains the characteristic expansion of polvilho azedo as a reaction of the lactic acid produced during fermentation and sun radiation. With the aim to evaluate a commercial continuous flow process, a modular lamp (UV-C) was evaluated to substitute exposure to sunlight. The selected doses were 1,000, 5,000 and 10,000 Joules per liter (J l-1). Native cassava starch suspension in water at 6% (dry basis) was adjusted to 4% lactic acid (dry basis) and exposed to UV-C radiation: (a) without lactic acid, (b) with lactic acid reaction for 1 hour, (c) with ultraviolet radiation, in addition to the combination of (ac) and (bc). The results showed that only the combination of acidification and ultraviolet radiation (bc) allows an expansion (11 cm3 g-1) higher than the commercial polvilho azedo (8.24 cm3 g-1) adopted as standard. It was concluded that is possible to achieve a substitute for fermented cassava starch by acidification of native cassava starch with lactic acid in a continuous flow system starting with a dose around 5,000 J l-1 of UV-C radiation.

Keywords:  Modified starch; Carboxyl; Cassava; Lactic acid; Quality

Resumo

O amido de mandioca fermentado (polvilho azedo) é valorizado como matéria-prima para o preparo de fast food. Sua produção tradicional por fermentação natural e exposição ao sol favorece a contaminação e impurezas, além de depender das condições climáticas. A literatura explica a expansão característica do polvilho azedo como uma reação do ácido lático produzido durante a fermentação e a radiação solar. Com o objetivo de avaliar um processo comercial em fluxo contínuo, uma lâmpada modular (UV-C) foi empregada para substituir a exposição à luz solar. As doses selecionadas foram 1.000, 5.000 e 10.000 Joules por litro (J l-1). A suspensão de amido de mandioca nativo em água a 6% (base seca) foi ajustada para 4% de ácido lático (base seca) e exposta à radiação UV-C: (a) sem ácido lático, (b) com reação de ácido lático por 1 hora, (c) com radiação ultravioleta, além da combinação de (ac) e (bc). Os resultados mostraram que apenas a combinação de acidificação e radiação ultravioleta (bc) permite uma expansão (11 cm3 g-1) superior ao polvilho azedo comercial (8,24 cm3 g-1) adotado como padrão. Concluiu-se que é possível obter um sucedâneo para o amido fermentado pela acidificação do amido de mandioca nativo com ácido lático em um sistema de fluxo contínuo a partir de uma dose em torno de 5.000 J l-1 de radiação UV-C.

Palavras-chave:  Amido modificado; Carboxilas; Mandioca; Ácido lático; Qualidade

1 Introduction

Among the products obtained from cassava, fermented cassava starch, known in Brazil as polvilho azedo, stands out as a type of modified starch that has as main attributes a characteristic flavor and expansion property (Cereda, 1993; Demiate & Kotovicz, 2011).

The expansion property of fermented cassava starch in the oven without using yeast or chemical leavening (baking soda) is unique. This expansion has attracted researchers not only because the snake’s preparation with it does not require extrusion, which is expensive, but also because it allows as an offer of baked products without gluten to the market (Brito & Cereda, 2015).

Fermented cassava starch was traditionally used at home or in small bakeries, but its main product, the cheese bread, became a fast food. It is a baked product which can be consumed with or without various fillings (Demiate & Kotovicz, 2011), which requires greater quality control.

The fermentation process yields organic acids with the predominance of lactic acid, which causes partial depolymerization of the polymer chains (Demiate et al., 1999). Exposure to solar ultraviolet (UV) radiation at the drying stage promotes oxidation and all of these modifications result in a plastic dough with baking expansion (Cereda, 1993; Plata-Oviedo & Camargo, 1998).

Fermented cassava starch is not uniform and presents contamination by dust, insects and microorganisms. When polvilho azedo is produced in small scale industries, it may show a variable quality (Brito & Cereda, 2015), which makes the constant search for improvements in the production process attractive (Vatanasuchart et al., 2005).

To explain polvilho azedo’s expansion, the hypothesis is that the reaction of starch with lactic acid catalyzed by UV radiation is instantaneous, but delayed by the slow infiltration of solar UV radiation into the fermented cassava starch on a drying surface. In small industries, the product is revolved by hand, and as this radiation has little penetration, only slightly thicker layers are exposed to solar UV radiation at a time (Cereda, 1993; Franco et al., 2010).

The published research results confirm the changes associated with oxidative degradation of starch by the concomitant use of lactic acid and artificial UV radiation, always provided by lamps set on surfaces where acidified cassava starch is exposed (Vatanasuchart et al., 2005; Marcon et al., 2009; Franco et al., 2010). Moreover, the batch process is difficult to apply at an industrial level and hinders quality standardization. The only way to measure the quality of cassava starch is to use a specific volume in cm3 g-1 (Brito & Cereda, 2015).

In the production of oxidized starches by a continuous system, Tethool et al. (2012) used a suspension of sago starch (Metroxylon sago Rottb) in hydrogen peroxide irradiated with UV (100 to 400 nm) for 15 minutes, having obtained a specific volume of 8.65 cm3 g-1 and an increase of 0.44% in carboxyl groups in the samples. Dewi et al. (2014) employed UV-C (100 to 250 nm) and found a higher carboxyl content (0.93%), but the expansion power of the baked dough presented much lower values (3.8 cm3 g-1).

This study evaluated a substitute for polvilho azedo produced in a commercial continuous flow system with lamps generating UV radiation type C (235-280 nm) in a suspension of 6% cassava starch in water adjusted to 4% of acid lactic (P.A.) on starch dry base and 1 hour as reaction time.

2 Materials and methods

2.1 Starch sample

We used two types of commercial cassava starch: Native starch from Amidos Paranaense® as the substrate of the tests and a sample of polvilho azedo Yoki® as the quality standard.

2.2 Equipment for UV-C treatment in a continuous flow system

For the treatment with ultraviolet (UV) radiation, a modular system of Germetec® Ultraviolet & Infrared Technology Ltda. (Rio de Janeiro - Brazil) was used. A commercial system (reactor + lamp) used for continuous treatment of water was selected. The inner wall of the lamp and the quartz glass outer wall forms a chamber through which the liquid to be treated flows. The reactor model GPJ-MP UV contains a medium-pressure mercury vapor arc lamp of 1,600 W, which emits radiation between 235 and 280 nm. Both the system and the starch treatment were registered under patent application BR10201402768.

In the modular system, it is possible to add or exchange lamps in sequence and adjust the radiation doses depending on the treatment duration. The lamp used has 32 mm in diameter and 335 mm in length, with an internal diameter of 50 mm and a length of 350 mm, providing a useful volume of 417.79 mL. A stainless-steel cover protects the set. The UV lamp lifetime is continuously marked at every use by a control panel. With the lamp power and the useful volume, the dose was expressed per liter of suspension considering the time in seconds to pass and recirculate the starch suspension through the lamp.

The suspension was placed in a plastic container (1) under mechanical agitation to keep the starch from settling. The dose of 1,000 Joules per liter (J l-1) corresponded to 48 seconds (or 1 lamp), the dose of 5,000 J l-1 corresponded to 240 seconds (or 5 lamps), and the dose of 10,000 J l-1, to 480 seconds (10 lamps).

2.3 Experimental design

The experimental design is organized in four treatments. The independent variables were cassava starch samples treatment with lactic acid and exposed to 3 doses of UV-C radiation. The dependent variable was the quality of the product, measured by expansion in the oven expressed by score of specific volume of baked dough (Brito & Cereda, 2015). A commercial fermented cassava starch was used as standard and a sample treated with acid lactic without UV-C treatment was used as whiteness.

The four treatments were: a) native cassava starch without acidification, (b) acid lactic and (c) 3 doses of ultraviolet radiation, in addition to compounds (ac) and (bc). The UV-C doses were calculated in 1,000, 5,000 and 10,000 J l-1.

All tests were conducted to simulate the conditions of the cassava starch industry, in which the starch slurry concentration in water is 6% on a dry basis (DB) after extraction and before the first centrifuge. The lactic acid concentration was established at 4% in relation to the native starch in dry matter. Therefore, for every 100 l of water, 6 kg of starch and 240 mL of Lactic Acid Synth® PA were used for 1 hour of reaction.

The 20-liter suspension was placed into the plastic container at 20 °C and the residence time was set to 1 hour. At the same time, the acidified suspension was introduced into the reservoir and partly fed by ultraviolet radiation treatment by a continuous flow pump.

2.4 Material preparation for characterization analyses

The starch slurry samples from each treatment were collected, filtered on paper filter using a Kitassato with the aid of a tight vacuum (Tecnal® TE-058). The wet mass retained was dried in an oven (Marconi® MA 037, Piracicaba - Brazil) with renewal forced air at 45 °C for 48 hours, followed by grinding in a mortar.

2.5 Expansion power expressed by specific volume

The specific volume was measured according to Brito & Cereda (2015). The mixture of starch and water was drawn directly on the silicon mold (Tupperware® capacity of 65 mL) at a proportion of 1:1, i.e. 5 mL of drinking water (pH 6.81) was added to 5 g of each starch sample (DB), and the mixture was homogenized with a spatula. The molds with the starch dough were placed directly into an electric oven of the Diplomat Autoforno Electric® brand at 200 °C for 20 minutes. The weight of the samples was obtained from a precision balance, and the volume, by seed displacement. The specific volume corresponding to the expansion power was calculated as the ratio of the volume (cm3) and weight (g) of each sample with the results expressed in cm3 g-1.

2.6 Characterization of the substitute of fermented cassava starch

The substitute of fermented cassava starch was characterized by the following analyses: humidity, in percentage (%), by the Official Methods Analysis (Association of Official Analytical Chemists, 1997); and hydrogen potential (pH) and carboxyl content, according to Smith & Paschall (1967), expressed as a percentage.

2.7 Rapid Visco Analyzer Viscosity (RVA)

The apparent viscosity of the starch samples submitted to the treatments was determined in Rapid Visco Analyzer (RVA-4 model, Newport ScientificTM, Narabeen, Australia). The samples (8% DB) were dispersed in deionized water with a metal beaker, followed by coupling to the system. The equipment was programmed to start the cycle at 50 °C and sit for 1 minute under this temperature. After this period, the sample was heated at a rate of 6 °C per minute to reach 95 °C, where it remained for 5 minutes. Then, the slurry was cooled to 50 °C (at a rate of 6 °C per minute) and remained at this temperature until the end of the analysis, which occurred 23 minutes afterwards as per the manufacturer's recommendations (Newport Scientific, 1998). The suspension was subjected to rotational shear at 160 rpm throughout the experiment. The properties of the starch pastes were determined using software Thermocline Windows® and the results were expressed in cP (centipoise).

2.8 Paste Transparence

Transparency was evaluated indirectly by transmittance (T%) using spectrophotometry at 650 nm according to Craig et al. (1989). To measure the transparency, a starch suspension was prepared in water at 1% (W:V, dry basis) and placed in a boiling water bath for 30 minutes. The samples were evaluated immediately (hot transparency) and after 24 hours (cold transparency) and the results were expressed as a percentage of transmittance. As standard, was used distilled water.

2.9 Statistical analysis

With the results with normal distribution, the mean and standard deviation (σ) were established. The means were compared by the Tukey test at 5% probability using the Statistica 7.0 software (StatSoft, Inc., 2008).

3 Results and discussion

Tables 1, 2 and 3 present the quality of the commercial polvilho azedo (standard), native cassava starch, and the other modified cassava starches. The same tables also present the characterization of the native and commercial fermented cassava starch and the effect of the variables on the physicochemical and functional properties of the cassava starches after the treatments. The native cassava starch presented the lower specific volume of all of the samples, corroborating the literature (Dias et al., 2007; Marcon et al., 2009; Brito & Cereda, 2015) that reports that the cassava starch has no expansion power without baking soda, yeast or extrusion.

Table 1 Expansion power by specific volume (DB), humidity, pH (DB), and carboxyl (DB) of native cassava starch, commercial polvilho azedo (fermented starch) and samples treated with lactic acid and/or ultraviolet (UV) radiation typeC in a continuous flow system. 

Sample Expansion Humidity pH Carboxyl
cm3 g-1 g 100 g-1 -log [H+] %
Native cassava starch 1.94 ± 0.25 c 10.53 ± 0.09 b 5.68 ± 0.04 c 0.00 ± 0.03 d
Commercial polvilho azedo 8.24 ± 1.65 a 10.60 ± 0.35 b 4.75 ± 0.01 d 0.16 ± 0.00 a
Starch acidified with lactic acid 4.62 ± 0.26 b 11.25 ± 0.25 b 3.18 ± 0.00 d 0.06 ± 0.00 c
Submitted to ultraviolet radiation type C without acidification
1000 J l-1 3.58 ± 0.47 c 13.65 ± 0.13 a 5.92 ± 0.00 c 0.09 ± 0,00 b
5000 J l-1 9.32 ± 3.14 a 8.30 ± 0.09 c 6.16 ± 0.09 b 0.11 ± 0.00 b
10000 J l-1 2.38 ± 0.40 c 10.55 ± 1,42 b 7.25 ± 0.00 a 0.06 ± 0,03 b
Submitted to ultraviolet radiation type C with acidification by lactic acid
1000 J l-1 9.34 ± 0.89 a 13.66 ± 0.21 a 4.23 ± 0.01 d 0.08 ± 0.03 b
5000 J l-1 11.29 ± 1.47 a 13.68 ± 0.20 a 4.62 ± 0.00 d 0.09 ± 0.03 b
10000 J l-1 11.99 ± 0.95 a 13.13 ± 0.06 a 4.88 ± 0.04 d 0.14 ± 0.03 b

In each column, the means values followed by the same letter do not differ by the Tukey test at 5% probability.

Table 2 Critical points of paste viscosity (8% DB) of native cassava starch and polvilho azedo (fermented starch), and samples treated with lactic acid and/or ultraviolet radiation type C in a continuous flow system. 

Sample Viscosity (cP)
Peak Break Retrogradation tendency
Native cassava starch 2782 ± 1.98 a 851 ± 3.07 a 1677 ± 0.17 a
Commercial polvilho azedo 1233 ± 0.46 b 380 ± 1.67 c 573 ± 3.33 d
Starch acidified with lactic acid 849 ± 0.33 b 80 ± 2.95 d 135 ± 8.94 e
Submitted to ultraviolet radiation type C without acidification
1000 J l-1 2990 ± 0.64 a 851 ± 2.07 a 1670 ± 0.38 a
5000 J l-1 2339 ± 0.18 a 1071 ± 3.43 a 1670 ± 1.83 a
10000 J l-1 2161 ± 1.11 a 676 ± 0.98 b 1179 ± 2.22 b
Submitted to ultraviolet radiation type C with acidification by lactic acid
1000 J l-1 2802 ± 0.91 a 310 ± 2.5 c 821 ± 2.32 c
5000 J l-1 2083 ± 1.77 a 394 ± 3.05 c 599 ± 8.50 d
10000 J l-1 1238 ± 2.74 b 315 ± 10.98 c 528 ± 13.25 d

In each column, means followed by the same letter do not differ by Tukey test at 5% probability.

Table 3 Transparency (1% DB) of native cassava starch and polvilho azedo (fermented starch) and samples treated with lactic acid and/or UV radiation type C in a continuous flow system. 

Sample Transparence (T%)
Paste at 80 °C Paste at 20 °C
Native cassava starch 44.47 ± 0.06 dA 43.63 ± 0.06 eA
Commercial polvilho azedo 63.20 ± 0.00 bA 62.10 ± 0.00 bA
Starch acidified with lactic acid 72.90 ± 0.00 aA 71.00 ± 0.00 aA
Submitted to ultraviolet radiation type C without acidification
1000 J l-1 32.10 ± 0.00 eB 41.73 ± 0.06 eA
5000 J l-1 55.73 ± 0.00 cA 54.73 ± 0.00 cA
10000 J l-1 55.50 ± 0.00 cB 59.30 ± 0.00 cA
Submitted to ultraviolet radiation type C with acidification by lactic acid
1000 J l-1 55.90 ± 0.00 cA 50.00 ± 0.00 dB
5000 J l-1 55.73 ± 0.00 cA 54.73 ± 0.00 cA
10000 J l-1 63.30 ± 0.00 bA 56.70 ± 0.00 cB

There was no significant difference in the same lowercase letters in the column for the treatments and the same uppercase letters in the line, for the paste temperature used, by Tukey test at 5% probability.

The expansion power was considered as the quality index for fermented cassava starch, and the aspect of the baked dough is also an important characteristic. Table 1 shows that the commercial fermented cassava starch presented the higher specific volume, similar only to samples treated with lactic acid under UV-C radiation in all of the doses evaluated.

The native starch (NS) sample presented no expansion (Table 1), as shown by the mark left at the base of the silicone mold in all of the baked doughs, with differences in patterns only at the top as an effect of the treatments. The sample of commercial polvilho azedo (PA) presented more vertical expansion with a slight deformation at the center and the top. The samples treated only with lactic acid (LA) presented a small volume and rounded shape. Similar shapes but with highest values are also visible in the samples treated only with UV-C radiation at doses of 1,000 and 5,000 J l-1. The research data do not yet allow to understand these differences.

Only the sample treated with lactic acid followed by radiation presented an expansion power equivalent to that of the commercial polvilho azedo (PA) with an expansion shape as a mushroom both horizontally and vertically. The dose of 5,000 J l-1, although providing expansion, presented a misshapen appearance by surface deformation. The sample from the treatment with both lactic acid and 5,000 J l-1 presented the best appearance, with a characteristic constriction in the center.

The use of extreme radiation doses (1,000 or 10,000 J l-1) alone on native starch caused an insufficient effect. These results corroborate the results found in the literature about the influence of ultraviolet radiation on the expansion and dough properties.

The values of expansion, shape, and appearance of the baked dough in the treatments with UV-C previously acidified with lactic acid starch did not differ from the standard commercial polvilho azedo. It is possible to conclude that the use of both lactic acid and UV-C treatment may develop the expansion power in native cassava starch.

Several authors have reported high values of expanding power in static systems; however, they reported the same values obtained with mechanized revolving of the starch to increase the surface under treatment. Most authors having researched fermented cassava starch improving process performed manual spreading of starch on surfaces. With this method, Franco et al. (2010) obtained an expansion power of 8.08 cm3.g-1 with 10 minutes of irradiation, whereas Vatanasuchart et al. (2003) obtained 12.23 cm3.g-1 for acidified cassava starch irradiated with UV type B or C; however, this result was achieved with seven hours of radiation exposure.

In the continuous system using the commercial equipment proposed in this work, an expansion of about 12 cm3 g-1 was also obtained; it is equivalent to the best values of the static system reported. This result was achieved with a significant shorter time of 240 seconds of UV-C treatment, which would be equivalent to five lamps in line. These results are superior to the 8.65 cm3 g-1 reported by Tethool et al. (2012), who worked with sago starch (M. sago) previously oxidized with hydrogen peroxide and treated with UV radiation in a continuous system afterwards.

Another problem affecting the comparison of results is the use of different methods to measure the expansion power—some still use complex raw materials, such as cheese and milk. To overcome this problem, in this work we used a newly described methodology, which is simpler and reproducible (Brito & Cereda, 2015).

With promising results for a continuous system, it is very important to characterize the substitutive obtained and compare it with the commercial polvilho azedo adopted as standard and with other substitutes. The results for characterization are shown in Table 1. The moisture content of the samples is not an important criterion to compare the substitutes because it can be adjusted after the treatment to comply with the current legislation, but the acidity pH shows a pattern that may represent the effect of the treatments.

The pH of native cassava starch was 5.68, and the commercial polvilho azedo adopted as standard showed low pH (4.75), which is a characteristic of the product and is related to the organic acids formed during fermentation (Brito & Cereda, 2015). In addition, the differences in acidy values observed for the treated samples are a consequence of deliberate acidification with lactic acid. But for the samples of cassava starch that received lactic acid and subsequently were submitted to UV-C treatment, acidity should not be altered; however, this was not the case. The samples submitted to the treatment with UV radiation in three doses presented a gradual and significant increase in the pH value compared to native starch (Table 1) due to lactic acid loss. The same phenomenon did not occur with the samples irradiated with UV-C which had been previously acidified (treatments b and c).

The pH values in the samples submitted to both acidification (b) and UV-C radiation (c) did not differ from that of commercial polvilho azedo but were significantly lower than the values presented by the native starch and higher than the ones found for the sample treated with lactic acid (a) alone. The lower values may be explained by the lactic acid molecule or a part of it grafting on glucose structure. A similar grafting effect was reported for acetic acid and starch reaction by Kumar et al. (2014).

The treatment with UV-C radiation caused an increase of 0.11% of carboxyl groups and 5.0% in specific volume, but 4.62 cm3 g-1 represents a big difference from the expansion of commercial fermented cassava starch adopted as standard. However, the carboxyl content does not follow the variation observed for the expansion power, reducing the importance of this analysis to explain the functional properties of polvilho azedo and its substitute, as show the results obtained by Dewi et al. (2014).

These results emphasize that only the expansion power characterizes the quality of commercial sour cassava starch and its substitute obtained in a continuous process. The other analyses (Table 2) only show that the variables may alter the functional properties as the viscosity caused by the treatment compared to the standard of commercial polvilho azedo.

The sample of native cassava starch was characterized by a peak viscosity of 1,233 cP, breaking of 380 and retrogradation tendency of 573 cP. The sample with the closest value was the one acidified and irradiated at 10,000 J l-1, which did not differ from the standard polvilho azedo, and therefore confirms the expansion results. The literature shows that only the acid treatment may affect the viscosity profile (Bertolini et al., 2000; Kumar et al., 2014), which may have been caused by partial depolymerization of starch after the treatment with lactic acid and photochemical reaction at a higher dosage. In the specific case of polvilho azedo and its substitutive, the molecular structural changes result in variations of the paste properties (Demiate et al., 2000; Vatanasuchart et al., 2003).

The treatment that most approximated the viscosity pattern of commercial fermented cassava starch was the one with lactic acid followed by UV-C radiation at 10,000 J l-1. This shows that although the viscosity is an important characteristic of starch and its application, it should not be considered as having a high impact on the quality factor.

The increase in viscosity of the samples treated with lactic acid followed by the application of UV radiation can be attributed to the great formation of hydrogen bonds by photocrosslinking (Fiedorowicz et al., 1999; Bhat & Karim, 2009) or a stronger type of link, which could also explain the baked dough pattern. The viscosity measurement seems to be more appropriate than the carboxylic analysis to evaluate the modifications of the starch in the production of a substitute for polvilho azedo. Due to the highest amount of carboxylic groups (Table 1), they tend to keep the chains separated, reducing the retrogradation tendency in the hot starch paste (Table 2) (Jyothi et al., 2005) and increasing the transparency property (Table 3). The transparency of the hot paste was greater than the cold one, which was expected and is explained by a structural reorganization of the amylose and amylopectin molecules in a retrogradation phenomenon (Hoover, 2001; Silva et al., 2008).

As to the results obtained for pH variation, the samples followed the same order when UV radiation was used, which allows us to conclude that the higher the UV-C dose, the higher the transparency of the paste, both on hot and cold conditions, when retrogradation increases (Table 3).

The results point to the similarity of the pre-acidified cassava starch sample treated with UV-C, beginning at 5,000 J l-1, to the commercial fermented cassava starch. The speed at which the reaction occurred allows us to highlight the advantages of using a modular reactor equipped with lamp which is capable of generating UV radiation type C in a continuous flow system.

4 Conclusion

The results show that it is possible to obtain a substitute of polvilho azedo close to the commercial one by treating cassava starch with both lactic acid and UV-C irradiation in a continuous flow system.

It also shows that the carboxyl content can be a good indicator to evaluate the quality of modified starches by oxidizing, which is only a part of the modification that allows for the expansion of cassava fermented starch. In addition, the transparency and viscosity are only used to emphasize the modifications to the native starch, but do not allow for evaluation of the quality of the fermented cassava starch.

The expansion adopted as quality index showed that the lowest UV-C dose (1,000 J l-1) is enough to provide the native cassava starch with the volume equivalent to the standard commercial fermented cassava starch and allows for the selection of the 5,000 J l-1 dose by allowing a shorter time, which reduces costs for the industry.

With the continuous system, it is possible to avoid the problems of the traditional process of polvilho azedo with fermentation and exposure to sunlight. Its use will allow for standardization of batches and prevent contamination. Additionally, it will make possible to adjust the product to market needs, which may provide greater flexibility to industries with the possibility of including the commercial equipment between the two centrifuges on the cassava starch industry plants, which already exists in industries.

Cite as: Brito, V., Santos, R., Narcisa-Oliveira, J., Demiate, I., & Cereda, M. (2021). Continuous flow process to make fermented cassava starch substitute: I. Physicochemical and functional properties. Brazilian Journal of Food Technology, 24, e2020017. https://doi.org/10.1590/1981-6723.01720

Funding: Ministério da Ciência, Tecnologia e Inovação/ Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq Process 476216/2006-0) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) by the scholarship.

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Received: February 17, 2020; Accepted: September 29, 2020

*Corresponding Author: Vitor Brito, Universidade para o Desenvolvimento do Estado e da Região do Pantanal (UNIDERP), Departamento de Produção e Gestão Agroindustrial, Av. Alexandre Herculano, 1400, CEP: 79035-470, Campo Grande/MS - Brasil, e-mail: britovhs@gmail.com

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