Biological parameters of cattle ticks fed on rabbits

Parâmetros biológicos de carrapatos bovinos alimentados em coelhos

FULL ARTICLE

Biological parameters of cattle ticks fed on rabbits

Parâmetros biológicos de carrapatos bovinos alimentados em coelhos

Maria Alice Zacarias do Amaral^I,III; Márcia Cristina de Azevedo Prata^II; Erik Daemon^III; John Furlong^II

^IAnimal Experimentation Center, Oswaldo Cruz Institute - Fiocruz

^IIEmbrapa Dairy Cattle - EMBRAPA

^IIIFederal University of Juiz de Fora - UFJF

^{Corresponding author}

ABSTRACT

With the objective of encouraging the use of rabbits as alternative hosts for the cattle tick, four rabbits were infested on the ears and back. From the second day of infestation the developmental stages were observed. e duration of larval engorgement and changes were estimated between six and eight days in the region of the back and between 've and seven days in the pinna. e nymphal engorgement and changes occurred at approximately 10.80 ± 2.65 days in the dorsal and 11.00 ± 2.52 days in the ear, with the engorgement of adults and copulation occurring at 7.03 ± 2.45 days, on the dorsal region and 8.55 ± 1.82 days in the region of the pinna. e parasitic period ranged from 21 to 29 days (back) and from 23 to 30 days (pinna). e engorged females of the back and ear weighed on average 34.43 ± 18.73 and 36.30 ± 18.10 mg, respectively. e nutritional and reproductive e/ciency indexes were 17.38 ± 14.27 and 26.85 ± 17.13% (back) and 17.42 ± 12.22% and 30.70 ± 19.80% (pinna). Although not appropriate to maintain successive generations of Rhipicephalus (Boophilus) microplus, rabbits can be used for di:erent stages of engorgement of the ixodid, allowing experimental studies.

Keywords:Rhipicephalus (Boophilus) microplus, alternate host, biological parameters

RESUMO

Com objetivo de viabilizar o uso de coelhos como hospedeiros alternativos do carrapato bovino, foram infestados quatro coelhos nas orelhas e no dorso. A partir do segundo dia da infestação, foram observados os estágios de desenvolvimento dos carrapatos. A duração do ingurgitamento e muda larval foi estimada entre seis e oito dias na região do dorso e entre cinco e sete dias no pavilhão auricular. O ingurgitamento e muda ninfal ocorreram em aproximadamente, 10,80 ± 2,65 dias na região dorsal e 11,00 ± 2,52 dias no pavilhão auricular; e o ingurgitamento e cópula dos adultos ocorreu em 7,03 ± 2,45 dias, na região do dorso e em 8,55 ± 1,82 dias na região do pavilhão auricular . O período parasitário variou de 21 a 29 dias (dorso) e de 23 a 30 dias (pavilhão auricular). As fêmeas ingurgitadas do dorso e orelha pesaram, em média, 34,43 ± 18,73 e 36,30 ± 18,10 mg, respectivamente. Os índices de e'ciência reprodutiva e nutricional foram de 17,38 ± 14,27 e 26,85 ± 17,13% (região do dorso) e de 17,42 ± 12,22% e 30,70 ± 19,80% (pavilhão auricular). Embora não sejam adequados para manter sucessivas gerações de Rhipicephalus (Boophilus) microplus, os coelhos podem ser empregados para o ingurgitamento dos diferentes estádios deste ixodídeo, viabilizando estudos experimentais.

Palavras-chave:Rhipicephalus (Boophilus) microplus, hospedeiro alternativo, parâmetros biológicos

Introduction

Infestation by Rhipicephalus (Boophilus) microplus causes serious economic problems to Brazilian cattle herds. Several authors have reported losses caused by ticks, including lesions of the scalp, decreased productivity, disease transmission, and the cost of acaricide acquisition and application, as well as the need for animal handling facilities. Consequently, there is constant need for investigations relating to controlling this species. Currently, most studies using experimental infestations in cattle are a relatively expensive activity. In this regard, if it were feasible to use rabbits, this would be advantageous due to the lower requirement for physical space, ease of handling and lower costs, thus di:ering considerably from cattle (SRIVASTAVA et al., 1986; GUIMARÃES et al., 1996). In continuation with research carried out previously we sought in this present study to observe the parasitic period and 'll in gaps in the knowledge about the nonparasitic period from evaluation of the biological performance of R. (B.) microplus in body regions of rabbits, in order to facilitate use of rabbits as an alternative host in experimental studies involving this tick species.

Materials and Methods

In the parasitic phase, experimental infestations were established using larvae of R. (B) microplus in four rabbits (Oryctolagus cuniculus). Each animal was inoculated in the ear and back, which constituted the experimental treatments. In each of these regions, 800 infesting larvae were placed as recommended by Guimarães et al. (1996). Containment devices were attached to the upper back and ears, adapting the technique used by Neitz et al. (1971). After each daily collection of the engorged females that had dropped, they were then weighed on an analytical scale and identi'ed in a Petri dish (Figure 1), while kept in a BOD incubator at a temperature of 27 ± 1 ºC and relative humidity of greater than 80%. ey were then observed to detect the onset of oviposition. Twenty-four hours after the onset, (Figure 2) the eggs were collected, weighed and packed in syringes, and then identi'ed and kept in an incubator to monitor the larva hatching process. roughout the laying period, at intervals of 24 ± 1 hours, the egg masses from each female were weighed and transferred to syringes, which were the experimental unit for the processes relating to the incubation and hatching of the larvae. ree days after the end of laying, the females were weighed in order to ascertain the weight loss that had occurred during the process, so that the nutritional e/ciency ratio (NER) could be calculated (BENNETT, 1974). Based on this methodology, we considered each body region to be a treatment area, each tick to be an experimental unit for the parasitic and laying phases, and every syringe containing egg masses from each female to be an experimental unit for the incubation and hatching processes. Using this approach, data on biological parameters were then gathered. e parameters for the period of engorgement and larval changes were the estimated number of days between infestation and the day of obtaining modal nymphs in each body region. e parameters for the period of nymphal engorgement and changes were the weighted average number of modal days taken to obtain nymphs and view every adult in each body region. e parameters for the period of engorgement and mating of adults were the weighted average number of modal days taken to obtain adults and the fall of each female in each body region. e parameters for the percentage recovery of engorged ticks were the percentage of engorged females obtained in relation to half the total number of larvae infesting (number of larvae that would give rise to females, considering the gender ratio of 1:1), pre-laying period (PPP), spawning period (PP), weight of egg mass/female (mg), weight of females at the beginning and end of laying (mg), weight of females three days after laying, incubation period (IP) in each experimental unit (syringe laying of females), percentage hatchability (% E), visual estimation of hatched larvae in relation to the total corresponding to the eggs laid by each female, laying rate (every 24 hours), reproductive e/ciency index (IER) and nutritional e/ciency ratio (NER), as described by Bennett (1974).

Statistical analysis was performed on the data, consisting of Student’s t test at 5% to investigate whether there were any signi'cant di:erences in the biological parameters of the ticks, as determined by local engorgement. If the sampling was characterized as lacking normal distribution, parametric Student’s t tests were replaced by the nonparametric Mann-Whitney test. Values expressed as percentages were transformed into the arcsine root prior to statistical tests.

Results and Discussion

Two hundred forty nine engorged females of R. (B.) microplus were recovered: 10.69% (171) from the animals’ ears and 4.88% (78) from their backs. is low female recovery rate may be associated with intraspecies competition between ticks for the physical space needed, as previously observed by other authors (GUIMARÃES et al., 1996). Another factor that may have in\uenced the low tick recovery is that the animals can drop out of the back restraining device through mechanical reactions from the hosts (BITTENCOURT et al., 1990; DAEMON et al., 1998). Cunha (1978) observed that adult females had died because the in\ammatory reaction had interfered with the blood supply to tissues. Guimarães (1995) found experimentally that the in\ammatory reaction was worsened as the number of infesting larvae increased, and suggested that as the quantity of parasites rose, the toxins in the tissue also rose, thereby increasing the degree of allergic reaction interfering with instar development.

The process of observing the changes and engorgement of the larvae during development of R. (B.) microplus was hampered by di/culty in viewing them because of their small size and the large number of specimens, and these data were only estimated. However, despite this, it is believed that such information may contribute towards further research involving this parasite. It is noteworthy that some parameters for the nonparasitic and parasitic stages of R. (B.) microplus, with rabbits as alternative hosts, were 'rst described in the complementary work by Guimarães et al. (1996).

Table 1 shows the duration of the larval engorgement process. is change was estimated to take between six and eight days on the dorsum and between 've and seven days in the ear region. Larger quantities of specimens were obtained at the upper limits of each range, and modal days were considered for such processes in each body region. In the literature, there is no data on the duration of each parasitic stage of R. (B.) microplus in rabbits, in order to make comparisons. In this regard, the present study is a pioneer. According to Tate (1941), the larval engorgement and changes in goats took 7 to 18 days, and in sheep, 7 to 12 days, for the larval stage. In experiments on dogs, the same author found that the changes took 8 to 16 days, for the larval stage. In the experiment by Daemon et al. (1998), larval engorgement and changes in goats took 5 to 7 days. e duration of the larval stage in cattle was 5.5 days (HITCHCOCK, 1955a). From analysis on these records, we were able to con'rm that the parasitic larval stage became extended in alternative hosts, possibly through adaptation to host species.

The nymphal engorgement and changes occurred in approximately 10.80 ± 2.65 days in the dorsal region and 11.00 ± 2.52 days in the pinna region. In goats, this process can occur in 7 to 27 days (TATE, 1941; DAEMON et al., 1998). In sheep, the nymphal stage ranged from 7 to 13 days, and in dogs from 8 to 16 days (TATE, 1941). In cattle, the duration of the process was shorter: around 7 to 8 days (HITCHCOCK, 1955a), con'rming previous information on the need for greater adaptation for alternate hosts.

In the present study, engorgement and mating of adults occurred in 7.03 ± 2.45 days on the dorsum and 8.55 ± 1.82 days in the pinna region. In a study on goats, Daemon et al. (1998) obtained an average value of 6.7 days. In cattle, this period was eight days (HITCHCOCK, 1955a). From analysis on these values, it was found that the host species has a considerable and signi'cant in\uences on the immature stages. ere was no evidence for a parasitic phase in the adult Rhipicephalus (Boophilus) microplus.

It was observed that the range for the parasite sampling period in the dorsal region was 21 to 29 days, and it was 23 to 30 days in the ear region. us, there was no signi'cant di:erence between the averages of 25.97 and 26.72 days, respectively, found from the back and ears. ese results are also similar to those found in rabbits (24-31 days) by Guimarães et al. (1996). Tate (1941) obtained parasitic periods in goats (20 to 38 days) and sheep (20 to 37 days) with greater range than what was found in the present work. e range in dogs (22 to 29 days) was similar to what was recorded in rabbits. Daemon et al. (1998) found a shorter average duration of the parasitic phase in goats (22.5 days) than in the present study.

The parasitic period for ticks on cattle reported in the literature is 21.9 days (HITCHCOCK, 1955a), i.e. shorter than the average periods of the present study (25.97 and 26.72 days), thus con'rming the previously mentioned e:ect of the host species on the duration of the change process of R. (B.) microplus. Out of the 78 engorged females (Table 2) recovered from the dorsum, 14 died after weighing, between the 'rst and third day after falling o: the host, before laying commenced. It is believed that this must have occurred due to early detachment of females because of competition among individuals. e same occurred with the females collected from the ear: out of the 171 females, 38 died over the 'rst days, before laying started.

The mean weights of the engorged females were 34.43 and 36.30 mg for the experimental groups from the back and ear, respectively, i.e. showing no signi'cant di:erences at the 5% level. is result allows us to deduce that these two regions o:er similar conditions for feeding and parasitism. However, there are anatomical and physiological di:erences between the two regions, given that the back is a \at region and thus would theoretically tend to facilitate larva attachment and development to the stage of engorged females. Guimarães et al. (1996) found that ear infestation in rabbits, using 800 larvae, produced engorged females with an average weight of 38.88 mg, similar to what was obtained in the present work, within the two body regions. e similarity of results between the two experiments is due possibly to the fact that they used the same methodology, in addition to having used the same host species with similar age and race.

It was observed that the weights of the engorged females were lower than those found in the study by Daemon et al. (1998). Although lower, these were similar to what was obtained with females hosted on cattle (DAVEY et al., 1980; BITTENCOURT et al., 1990; GLÓRIA et al., 1993; BORGES et al., 2001). From analysis on these records, it can be argued that use of rabbits as an alternative host for engorgement of females in the laboratory is a viable alternative when there is no requirement regarding the weight of engorged females. is re\ects the in\uence of host species, based on factors such as the immune system (SRIVASTAVA et al., 1987), the host’s defense mechanism (licking, scratching and immune response) (CASTRO; PEREIRA, 1946) and resistance due to blood levels and nutrition (OLIVEIRA; ALENCAR, 1990; MANGOLD et al., 1994; VIANNA et al., 1995).

The pre-laying periods were 3.85 ± 1.80 and 3.39 ± 1.14 days for the back and ear respectively. ese periods were similar, thus showing that the time required for the engorged females to begin laying is irrespective of the body region. Furthermore, this parameter seems not to be in\uenced by the host species in question, given that studies on the nonparasitic phase of R. (B.) microplus in cattle and goats produced results similar to those of the present work (HITCHCOCK, 1955b; ALVARADO; GONZALES., 1979; DAVEY et al., 1980; BITTENCOURT et al., 1990; GLÓRIA et al., 1993).

The oviposition periods of the females on the back (5.55 days ± 1.88) and ears (5.17 days ± 2.05) were statistically similar to what was found by Guimarães et al. (1996), who also used rabbits ('ve to seven days). ese periods were shorter than those recorded in experiments with goats (DAEMON et al., 1998) and cattle (HITCHCOCK, 1955b). is di:erence in the number of laying days between females on rabbits and other species can be explained by the weight of the engorged female. Rabbits present low numbers because there is a correlation between the weight of the engorged female and the number of eggs laid. erefore, smaller females lay fewer eggs, thus taking fewer days for the process, as described by Santos and Furlong (2002).

The weight of the eggs, the residual weight of the females and the indices of reproductive e/ciency and nutrition in the two groups are presented in Table 2. ere was a range from 0.45 to 35.30 mg of the weight of the egg mass from the back, with an average of 6.76 mg, which is statistically similar to the average of 6.90 mg obtained from the pinna region, where the range was from 0.51 mg to 36.45 mg. is similarity can be explained by the fact that the weights of the engorged females in the two regions were similar, thus con'rming the aforementioned direct relationship between the weight of engorged females and number of eggs laid (SANTOS; FURLONG, 2002). e weight of egg masses reported in the literature concerning the tick R. (B.) microplus on cattle and the weight of females were higher than those of the present study (BITTENCOURT et al., 1990; GLÓRIA et al., 1993). is con'rms the statement about the close relationship between these two parameters (weight of engorged females and egg weight). According to Borges et al. (2001), measures that increase the weight of engorged females increase the weight of egg masses (SANTOS; FURLONG, 2002).

The average weights of the females at the end of oviposition, from the dorsal and ear, were 16.04 ± (0.70-48.50) and 17.72 ± (1.06 to 91.60) mg, respectively. ese can be considered statistically similar. ese weights were much lower than those found in the literature for R. (B.) microplus in goats (62.1 and 67.4 mg in the 'rst and second generations respectively) (DAEMON et al., 1998) and cattle (63.60 mg) (GLÓRIA et al., 1993) due to the small size of the engorged females from rabbit hosts. e indices of reproductive e/ciency and nutrition were also low. e range obtained for the 'rst parameter was 1.95 to 89.37% for the dorsum and 2.30 to 88.50% for the ear region, with no statistically signi'cant di:erence between the means of 17.38 and 17.42% obtained from the respective regions. e mean values di:ered signi'cantly from the second parameter: 26.85 and 30.70% respectively from the dorsal region and ears, and these were found to range from 1.02 to 89.90% and from 2.56 to 96.33%.

Both regions produced low engorgement, egg production was signi'cantly lower and the average weights of the egg masses were 6.76 mg from the back and 6.90 mg from the ears. ese re\ect low rates of reproductive e/ciency, thus demonstrating, in the case of this population, low reproductive capacity after engorgement. A similar situation occurred in relation to the nutritional e/ciency index. However, the average weight loss of females at the end of laying did not reach the 74.32% recorded by Alvarado and Gonzales (1979) in their experiment. e blood processing capacity in eggs was low in this study, and it can therefore be assumed that there was considerable deviation of body mass for other metabolic processes, thereby characterizing a situation of discomfort and/or stress for the ticks. Although both these parameters were considered low (IER and NER of 17.38 and 26.85%), compared with 'ndings in the literature on rabbits (IER 29.10% and NER 50.32%) (GUIMARÃES et al., 1996), these results can be correlated with a possible di:erence between populations of the same species. Morphological di:erences relating to tick provenance have been observed (FAMADAS; FACCINI, 1989). ese di:erences may be caused by several factors, such as selection pressure due to acaricides (FURLONG et al., 2004) and geographical isolation, among other causes. ese factors modify the pro'le of a population and consequently, the biological parameters. In relation to the other alternative host (goats), both indices in the present study were found to be low. is parameter may have been in\uenced by the fact that because goats are physiologically similar to the preferred host species for ticks (cows), they provide conditions that are more suitable than those of rabbits. e indices of nutritional and reproductive e/ciency obtained in this study were also lower than those recorded from cattle-hosted ticks by Alvarado and Gonzales (1979) and Glória et al. (1993). Again, these results may have been caused by di:erent factors, but the main factor is the use of hosts that can be considered to be more suitable than rabbits for development of the tick R. (B.) microplus.

Among the secondary causes, it should be mentioned that the host reaction is individual, regardless of the species concerned (CASTRO; PEREIRA, 1946). It was found in rabbits infested with R. (B.) microplus that the in\ammatory reactions caused by tick bites meant that the larvae could not have access to any source of blood. ey therefore fed on substances resulting from the lysis that occurs in the area of acute cellular in'ltration. is would lead to engorgement of larvae with a pallid hue, while retaining the ability to make changes and thereby leading to production of light females with low reproductive performance (CUNHA, 1978; GUIMARÃES, 1995).

Regarding the oviposition rate of the females fed on the two body regions, the average daily peak egg production was reached between the second and third days after the onset of laying. In this range, the total masses of eggs laid ranged from 14.80 to 14.50% for females on the back, and from 16.70 to 16.12% for females on the pinna. e peaks were not so pronounced, with higher concentration of the metabolism between the second and sixth days, regarding the position of females on the dorsal region and between the second and fourth days on the ear.

The periods required for 50% of the egg mass to be laid were three and four days, respectively, in the ear and back regions. It was observed that about 90% of the total average oviposition had been laid by the eighth day in the ear region, and by the ninth day on the dorsum, thus suggesting that the metabolism in the laying process was faster among females on the pinna. It also appears that the curve of the number of eggs laid daily showed similarities with those observed in experiments using di:erent host species conducted by Hitchcock (1955b), Davey et al. (1980) and Glória et al. (1993), with maximum numbers in the early days of laying.

Table 2 presents a range from 19 to 26 days for the incubation period for eggs laid by females in the two regions, thus resulting in a signi'cant di:erence between the averages, of 22.70 and 23.40 days for the back and ears respectively. e di:erence between the averages for the two regions re\ects the need for a longer incubation period for the eggs from the engorged females on the ear to complete the process, and then for the larvae to start to hatch. is faster metabolism of incubation on the back suggests the possibility that an o:set may exist due to the slower metabolism developed during the laying in this region. From analysis on the results presented in relation to the incubation period, there were also changes in the non-parasitic phase, possibly promoted by local engorgement of the ticks (parasitic phase). e reverse process, i.e. changes in the parasitic phase determined by the maintenance conditions during the nonparasitic phase, was noted by Bellato and Daemon (1997) (Rhipicephalus sanguineus) (Latreille, 1806) and Chacón et al. (2002) (A. cajennense). The averages obtained for the incubation period were lower than those recorded with cattle-hosted ticks (23 to 25 days) (DAVEY et al., 1980; BITTENCOURT et al., 1990; GLÓRIA et al., 1993). This difference may be explained by the host species, and a series of factors such as the body region where the host was parasitized and/or the methodology used in each experiment.

No significant difference in the percentage of hatching was observed between the regions. The range obtained was from 5 to 90% (back) and 5 to 95% (pinna), with averages of 35.75 and 34.34% for the back and the pinna. These values were lower than those found by Guimarães et al. (1996), who found a rate of 96.65%. These low values may be a consequence of the aforementioned factors that are responsible for differences in population profiles, which change the biological parameters.

Conclusions

From analysis on the results, we can conclude that the host species has a clear influence on the parasitic period of the larvae and nymphs of R. (B.) microplus. The use of rabbits in experimental studies to engorge R. (B.) microplus is feasible, but with reservations, depending on the type of study that will be performed, for example, when there is no requirement regarding the weight of the engorged females.

Received March 21, 2011

Accepted February14, 2012

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Publication Dates

• Publication in this collection
  23 Apr 2012
• Date of issue
  Mar 2012

History

• Received
  21 Mar 2011
• Accepted
  14 Feb 2012