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Revista Brasileira de Parasitologia Veterinária

Print version ISSN 0103-846XOn-line version ISSN 1984-2961

Rev. Bras. Parasitol. Vet. vol.23 no.3 Jaboticabal July/Sept. 2014

https://doi.org/10.1590/S1984-29612014061 

Review Article

Comparative morphology of the species of Libyostrongylus and Codiostomum, parasites from ostriches, Struthio camelus, with a identification key to the species

Morfologia Comparativa das espécies de Libyostrongylus e Codiostomum, parasitas de avestruzes, Struthio camelus, com uma chave para identificação das espécies

Nicole Brand Ederli1  * 

Francisco Carlos Rodrigues de Oliveira2 

1 Campus Universitário do Marajó-Breves, Universidade Federal do Pará – UFPA, Breves, PA, Brasil

2 Laboratório de Sanidade Animal, Hospital Veterinário, Universidade Estadual do Norte Fluminense – UENF, Campos dos Goytacazes, RJ, Brasil


ABSTRACT

One of the most common problems in breeding of ostriches in captivity is the control of parasitic diseases. This work presents keys for the identification of adult nematodes and infective larvae by morphologic and morphometric characteristics. These keys will allow the scientific community to identify the species that infect the ostriches either based on the characteristics of the posterior end of the infective larvae found through a simple fecal exam or by observing the morphology and morphometry of adult worms recovered during necropsies. These keys will facilitate ecological and systematic studies, as well as increase the understanding of the epidemiology of these parasitosis in ostriches.

Key words: Ratites; Nematoda; Strongylida; infective larvae

RESUMO

Um dos problemas mais comuns na criação de avestruzes em cativeiro é o controle das doenças parasitárias. Este trabalho apresenta chaves para a identificação de Nematoda adultos e larvas infectantes através de caracteres morfológicos e morfométricos. Essas chaves de identificação permitirão à comunidade científica o diagnóstico das espécies que infectam as avestruzes com base nas características da extremidade posterior das larvas infectantes encontradas por meio de simples exames fecais ou pela observação da morfologia e morfometria dos espécimes adultos recuperados durante necropsia. Dessa forma, as chaves de identificação facilitarão os estudos ecológicos e sistemáticos, bem como a melhor compreensão da epidemiologia dessas infecções em avestruzes.

Palavras-Chave: Ratitas; Nematoda; Strongylida; larva infectante

The ostriches, Struthio camelus Linnaeus, 1758 are birds belonging to the group of the ratites. These birds originated from Africa, and currently, their commercial breeding has gained economic importance worldwide due to the ability of these birds to adapt to different environments and their lucrative agricultural potential (TULLY; SHANE, 1996). One of the most common problems in breeding ostriches in captivity is the control of parasitic diseases, particularly those caused by parasites that have a direct lifecycle (HUCHZERMEYER, 2005). A diverse helminthic fauna has been reported in ostriches (Table 1), of these, the nematodes are important organisms that limit the development of the ostrich flock.

Table 1. Main parasites reported parasitizing ratites (ostrich, Struthio camelus; Rhea, Rhea americana; Emu, Dromaius novaehollandiae; Cassowary, Casuarius casuarius; Kiwi, Apterys spp.). 

Parasites Site of Infection Host Author (s)
Protozoa
Balantidium sp. Caecum/Intestine Ostrich, Rhea 1, 2
Cryptosporidium Intestine All 1, 3, 4
Eimeria spp. Intestine All 1, 3
Entamoeba sp. Caecum/Intestine Ostrich, Rhea 1
Giardia Caecum/Intestine Ostrich, Rhea, Emu 1, 3
Histomonas meleagridis Liver Ostrich, Rhea 1, 3, 5
Isospora spp. Intestine Ostrich 1, 3
Leucocytozoon struthionis Blood Ostrich 3
Plasmodium struthionis Blood Ostrich 3
Pleuromonas Large Intestine Ostrich, Rhea 1
Tetratrichomonas Caecum/Intestine Ostrich, Rhea 1
Toxoplasma gondii CNS All 3
Trichomonas Esophagus Ostrich, Emu 3
Trematoda
Fasciola sp. Liver Rhea, Emu 6, 7
Philophthalmus gralli Eyes Ostrich 3
Philophthalmus aweerensis Eyes Rhea 8
Cestoda
Chapmania tauricolis Small Intestine Rhea 9
Davainea spp. Small Intestine Rhea, Emu, Cassowary 3
Houttuynia struthionis Small Intestine Ostrich, Rhea 1, 3, 10
Monoecocestus sp. Small Intestine Rhea 11
Raillietina spp. Intestine Emu 12
Nematoda
Ascaridia orthocerca Intestine Rhea 3
Baylisascaris sp. CNS (Larvae) Ostrich, Rhea 3
Capillaria spp. Intestine Ostrich, Rhea 1, 13
Chandlerella quiscali CNS (larvae) Emu 3
Codiostomum struthionis Caecum Ostrich 3, 14
Cyathostoma variegatum Lungs/Trachea Emu 15
Cyrnea colini Proventriculum/Gizzard Ostrich 3
Deletrocephalus dimidiatus Large Intestine Rhea 3, 11, 16, 17, 18
Deletrocephalus cesarpintoi Large Intestine Rhea 3, 9, 17, 19
Dicheilonema rheae Connective Tissue Ostrich, Rhea 3, 20
Dromaestrongylus bicuspis Small Intestine Emu 3
Habronema incerta Proventriculum/Gizzard Rhea 3
Libyostrongylus spp. Proventriculum/Gizzard Ostrich 1, 3, 21, 22, 24
Odontospirura cetiopenis Proventriculum/Gizzard Rhea 25
Paradeletrocephalus minor Large Intestine Rhea 17, 26
Paranchocerca struthiononus Lungs Ostrich 3
Sicarius uncinipenis Gizzard Rhea 9, 27
Sicarius waltoni Gizzard Rhea 27
Struthiofilaria megalocephala Thoracic cavity Ostrich 3
Syngamus trachea Trachea Ostrich, Rhea, Emu 3
Torquatoides crotophaga Gizzard Rhea 9
Vaznema zschokkei Proventriculum Rhea 27

Authors: 1Ponce Gordo et al. (2002); 2Ederli and Oliveira (2008); 3Foreyt (2005); 4Oliveira et al. (2008); 5Dhillon (1983); 6Soares et al. (2007); 7Vaughan et al. (1997); 8Schuster (2011); 9Zettermann et al. (2005); 10Pintori et al. (2000); 11Giossa et al. (2004); 12O’Callaghan et al. (2000); 13Railliet and Henry (1911); 14Ederli et al. (2008c); 15Rickard et al. (1997); 16Monteiro et al. (2002); 17Freitas and Lent (1947a); 18Travassos (1933); 19Vaz (1936); 20Vakarenko and Sharpilo (2000); 21Ederli et al. (2008); 22Ederli et al. (2008a); 23Bonadiman et al. (2006); 24Hoberg et al. (1995); 25Wehr (1934); 26Acomolli et al. (2006); 27Freitas and Lent (1947b).

In 1880, small nematodes were collected from an ostrich proventriculum in South Africa by Arthur Douglass and sent to Dr. Cobbold, who identified the worms and named the new species Strongylus douglassii in honor of their collector (COBBOLD, 1882). Later, Lane (1923) established the genus Libyostrongylus for some African Trichostrongylidae, where L. douglassii is the type species from ostriches and L. hebrenicutus Lane, 1923, as the type species for gorillas. Gilbert (1937) described a new species L. magnus, being found to-date only in ostriches from the African continent, without recent reports in the world. In this species, unlike others of this genus, the females are smaller than males. Libyostrongylus douglassii and L. hebrenicutus differ in the structure of the terminal bifurcations of the dorsal ray and in the pattern of lateral rays (rays 3 to 5) of the copulatory bursa. Ortlepp (1939) established the genus Paralibyostrongylus; being P. vondwei Ortlepp, 1939, the type species and P. hebrenicutus, and P. nigeriae Baylis, 1928 included in this new genus (HOBERG et al., 1995). Hoberg et al. (1995) described a third species, named L. dentatus Hoberg, Lloyd and Omar, 1995, due to the presence of prominent esophageal teeth. Thus, the genus Libyostrongylus is actually comprised of 3 species: L. douglassii, L. magnus and L. dentatus. Of these, the most common species is L. douglassii, which has been reported in South African flocks (FOCKEMA et al., 1985; MALAN et al., 1988; REINECKE, 1983); Australia (BARTON; SEWARD, 1993; BUTTON et al., 1993; MORE, 1996); the United States (HOBERG et al., 1995); Italy (PINTORI et al., 2000); Scotland (PENNYCOTT; PATTERSON, 2001); Spain, Belgium, Portugal and the Netherlands (PONCE GORDO et al., 2002); Sweden (JANSSON et al., 2002); New Zealand (MACKERETH, 2004; MCKENNA, 2005); Zimbabwe (MUKARATIRWA et al., 2004) and Brazil (BONADIMAN et al., 2006; EDERLI et al., 2008a, b). There have been few reports of 2 other species of the genus Libyostrongylus. Libyostrongylus magnus has been reported only in South Africa (GILBERT, 1937) and L. dentatus only in the United States (HOBERG et al., 1995) and Brazil (BONADIMAN et al., 2006; EDERLI et al., 2008a, b). Because most reports are based only on fecal exams, the low reported rate of infections by these 2 species is probably due to errors in diagnosis because of the similarities in the infective larvae.

Data about the life cycle of the genus Libyostrongylus refer only to L. douglassii, which has a direct life cycle typical of Trichostrongylidae, with a pre-patent period of approximately 36 days (THEILER; ROBERTSON, 1915) (Figure 1). The eggs are shed in the feces of the infected hosts and develop in the environment into larvae of first (L1), second (L2) and third (L3) stages (infective larvae). This development occurs at a minimum temperature of 7 to 10°C and maximum of 37°C. Infective larvae display negative geotropism, tending to climb to the tip of vegetation through the moisture layer (MCKENNA, 2005). Under optimal temperature conditions (36°C), the development to infective larvae occurs in approximately 60 hours (BARTON; SEWARD, 1993). Ostriches become infected by the ingestion of the infective larvae, which develops into the fourth larval stage (L4) within the proventricular glands in a period of 4 to 5 days. After approximately 20 days, these larvae develop into juvenile larvae. After the maturation process, copulation occurs and the females release eggs in the proventriculum approximately 33 days after the infection. It takes about 4 days for eggs appear in the feces of the host (THEILER; ROBERTSON, 1915).

Figure 1. Life cycle of Libyostrongylus spp., according to Theiler and Robertson (1915). 

Another species often cited as a parasite from ostriches, although with few reports, is Codiostomum struthionis. It was originally described as Sclerostoma struthionis by Horst in 1885 (POPOVA, 1955). In 1911, Railliet and Henry reclassified this species, and established the genus Codiostomum, being C. struthionis the type species and the only species in the genus (RAILLIET; HENRY, 1911). Since that time, there have been an extremely small number of studies regarding this nematode. However, this species is cited in several scientific publications describing the similarity of its eggs with that of the genus Libyostrongylus (BARTON; SEWARD, 1993; BLACK, 2001; PENNYCOTT; PATTERSON, 2001; SOTIRAKI et al., 2001; PONCE GORDO et al., 2002; HUCHZERMEYER, 2002, 2005; MCKENNA, 2005; TIŠLJAR et al., 2007).

The life cycle of C. struthionis has not been determined. Oliveira et al. (2009) believe that these nematodes have a direct life cycle and the larvae migrate into the host tissue, appearing in the nodules found along the cecum of infected birds, however, this fact has not been confirmed.

According to Ponce Gordo et al. (2002), the occurrence of C. struthionis is often overlooked in Europe due to a possible confusion in the diagnosis by the observation of eggs in the feces because the eggs are similar to those of the genus Libyostrongylus, this misdiagnosis is probably happening on other continents.

There are few reports of the occurrence of C. struthionisinfecting ostrich flocks in South Africa (POPOVA, 1955); Europe (PONCE GORDO et al., 2002); Brazil (EDERLI et al., 2008c) and possibly in Greece, where Sotiraki et al. (2001) found eggs typical of the family Strongylidae in feces of ostriches but did not analyze fecal cultures to confirm the diagnosis. This low incidence of infection is probably due to misdiagnosis because of the similarity of C. struthionis eggs with those from the genus Libyostrongylus. Additionally, there is similarity between the infective larvae of C. struthionis and L. dentatus(EDERLI et al., 2008a, c).

Until recently, the specific diagnosis of these nematodes was possible only through the identification of adult worms collected during necropsies. Studies carried out by Ederli et al. (2008a) and Ederli et al. (2008c) allowed the distinction of C. struthionis, L. douglassii and L. dentatus through the observation of infective larvae recovered from fecal cultures, thus facilitating diagnosis.

Libyostrongylus spp. is considered the most pathogenic nematode, responsible for 50% of the mortality of juvenile birds and occasionally killing adults (REINECKE, 1983). Until recently, C. struthionis was considered a nonpathogenic parasite (HUCHZERMEYER, 2005); however, a recent study by Oliveira et al. (2009)verified that C. struthionis is responsible for macroscopic lesions in the ostriches' cecum, characterized as mucosal thickening nodules distributed throughout the cecum, and the presence of hemorrhages caused by small ulcers circumscribed to the mucosal edema. Due to these observations, these authors hypothesize that a high parasite load by C. struthionis could result in loss of fitness.

These parasites appear to be specific to ostriches; however, there was a single report of the occurrence of Libyostrongylus in emu, Dromaius novaehollandiae (Lathan, 1790) in Sweden (JANSSON; CHRISTENSSON, 2000; PONCE GORDO et al., 2002), which may have been due an error in diagnosis. The cross-transmission potential of these nematodes to other domestic birds (in particular, other ratites) has not yet been determined. Studies carried out by Zettermann et al. (2005) to detect helminthes in rheas, Rhea americana Linnaeus, 1758, showed that none of the 8 freeliving adult rheas or 12 captive juveniles rheas were infected by Libyostrongylus spp. or C. struthionis. They found 7 other species of parasites in the examined rheas: Sicarius uncinipenis (Molin 1860), Torquatoides crotophaga Williams, 1929, Deletrocephalus dimidiatusDiesing, 1851, D. cesarpintoi Vaz, 1936, Paradeletrocephalus minor Freitas and Lent, 1947, Capillaria venteli Freitas and Almeida, 1935 and Dicheilonema rheae (Owen, 1843). Gallo et al. (2010) conducted a study to evaluate the effects of an experimental infection of chickens by Libyostrongylus spp., where they infected normal and immunosuppressed one-day-old chicks with infective larvae of L. douglassii and L. dentatus; nonetheless, the infection was not established. In some birds they observed the elimination of unsheathed larvae in birds' feces from 1 to 5 days following the infection. Moreover, the presence of eggs of Trichostrongylidae was not observed until the end of the experiment (52 days). Because the unsheathed larvae cannot survive in the environment, chickens are not at risk for the transmission or for acting as a paratenic host. However, a possibility of transmission of these parasites to other birds, mainly other ratites, cannot be discounted.

The present article presents keys for the identification of the main genus (infective larvae and adults) in ostriches to facilitate diagnosis by fecal exams and the observation of adults recovered in necropsies.

Adult Nematodes

The parasites of the genus Libyostrongylus are small nematodes, of a reddish color in vivo and found in the proventriculus and gizzard of ostriches. The genus Libyostrongylusis cited as being found under the koilin layer (HOBERG et al., 1995; HUCHZERMEYER, 2005; EDERLI et al., 2008b); however, a recent study by Ederli and Oliveira (2009) observed differential localization of L. douglassii and L. dentatus within the proventriculum. Libyostrongylus douglassii is found on the mucosa just under the koilin layer, as described in the literature, while L. dentatusis found inserted into the koilin layer. The distinct localization of these 2 species in the proventriculum may be the reason there are so few reports of the presence of L. dentatus. Until the publication of these observations, L. dentatus was sought along with L. douglassii under the koilin layer, possibly resulting in false negative diagnoses for L. dentatus because the koilin layer is removed and discarded during the collection of the parasites. The description of the differential localization of these 2 species of nematodes in the proventriculum may result in an increase in the number of reports of L. dentatus.

The morphological description of L. magnus is published in Greek (GILBERT, 1937), which hinders its access. There are no further studies about the morphology of this species. Hoberg et al. (1995) was able to re-examined some specimens deposited in the International Institute of Parasitology (LSHTM 1317) which was previously identified as L. douglassii in 1933 in Ethiopia. Hoberg et al. (1995) identified those nematodes as L. magnus. According to Hoberg et al. (1995), the measurements of some male and female specimens of L. douglassii described by Durette-Desset and Denke (1978) from an ostrich in Somalia should classify them as L. magnus (Table 2). Unlike other species of the genus, the females of L. magnus are smaller than males and have a larger ovejector when compared with L. douglassii and L. dentatus(Table 2). Libyostrongylus douglassii and L. dentatus can be distinguished by the observation of some morphological characteristics. Libyostrongylus dentatus can be distinguished from congeners by the presence of 3 prominent esophageal teeth (Figure 2B). Both males and females of L. dentatusare longer than L. douglassii (HOBERG et al., 1995; EDERLI et al., 2008b) (Table 2). These 2 species also have differences in the morphology on the cephalic end: L. dentatus has a flat extremity with 3 pairs of rounded cephalic papillae, while L. douglassii has a rounded end with 3 pairs of elongated papillae (EDERLI et al., 2008b). Female L. dentatus can be distinguished from L. douglassii by its longer ovejector (Figures 2A and 3A) and by the presence of a cuticular inflation at the anal level (Figures 2C and 3C). Libyostrongylus magnus was described as having long ovejector, but it can be distinguished from L. dentatus by the larger number of eggs inside (Figure 4A) (SKRJABIN, 1961). Male L. dentatus can be distinguished by an asymmetric dorsal ray (Figure 2D), while other species have a symmetric dorsal ray (Figure 3D and 4D). Moreover, L. dentatushave spicules with the main shaft ending in a rounded point (Figure 2E), while the spicules of L. douglassii have a main shaft ending in a point (Figure 3E) (HOBERG et al., 1995; EDERLI et al., 2008b).

Figure 2. Libyostrongylus dentatus. (A) Ovejector; (B) Anterior end; (C) Female posterior end; (D) Male copulatory bursa; and (E) Spicules. 

Figure 3. Libyostrongylus douglassii. (A) Ovejector; (B) Anterior end; (C) Female posterior end; (D) Male copulatory bursa; and (E) Spicules. 

Figure 4. Libyostrongylus magnus (A) Ovejector; (B) Anterior end; (C) Female posterior end; (D) Male copulatory bursa; and (E) Spicules. Re-drawed from Skrjabin (1961). 

Table 2. Comparison of adult males and females from the species of genus Libyostrongylus Cobbold, 1882. Measurements in micrometers. 

Characters L. douglassii1 L. magnus2 L. dentatus1
Total length (Females) 3,340-5,120 7,560-11,430 7,192-10,450
Total length (Males) 2,240-4,928 13,860-14,580 4,752-5,156
Ovejector length 188-335 1,725 407-503
Spicules length 144-171 220-240 103-130
Prominent esophageal teeth Absent Absent Present
Cuticular inflation at anus level Absent Absent Present
Dorsal ray symmetry Symmetric Symmetric Asymmetric

1 Ederli et al. (2008b).

2Skrjabin (1961).

Codiostomum struthionis are large and robust nematodes, with white coloration in vivo, observed easily in the cecum of ostriches. These nematodes can be easily distinguished from the genus Libyostrongylus because of their greater length. Recently, this species had its morphology and ultrastructure redescribed by Ederli et al. (2008c). Codiostomum struthionis has a well-developed buccal capsule with 2 rows of corona radiata. Males have a strongly curved ventral copulatory bursa, with a large projection of the dorsal lobe and long and slender spicules. Females have prodelphic uteri and a pointed tail tip (EDERLI et al., 2008c).

To facilitate the differentiation of adult nematodes from the genus Libyostrongylus, an identification key is described below:

Infective Larvae

The infective larvae of the genus Libyostrongylus are characterized by the presence of a knob in the larvae tip tail (BARTON; SEWARD, 1993). Bonadiman et al. (2006) reported the presence of L. douglassii and L. dentatus in the northern region of the state of Rio de Janeiro, Brazil, by observing adult worms collected from the proventriculum during necropsies in 3 adult ostriches. They also observed differences in the length and morphology of the infective larvae sheath tail recovered from fecal cultures of samples from the same region. Some larvae had short sheath tail with an acute termination and others had a long and filamentous sheath tail. The authors described these differences as a possible way to distinguish these 2 species, as both were found parasitizing ostrich flocks in the analyzed region (BONADIMAN et al., 2006). Ederli et al. (2008a) confirmed this observation, showing the infective larvae of L. douglassii which has a short sheath tail (average of 29.52 µm ± 4.11; ranging from 20.62 to 36.31 µm), while the infective larvae of L. dentatus has a long sheath tail (average of 61.20 µm ± 2.17; ranging from 40.01 to 84.01 µm) (Figures 5A, B). Following this study, it was possible to differentiate these 2 species by the morphology and morphometry of the infective larvae sheath tail recovered from fecal cultures. Because it was no longer necessary to perform a necropsy to collect adult worms, specific diagnosis was facilitated. However, the infective larvae of C. struthionis also have a long and filamentous sheath tail (average of 110.74 µm ± 13.46, ranging from 85.87 to 143.18 µm), but the tail of the larvae inside the cuticle has an acute termination (Figure 5C) (EDERLI et al., 2008c), which is different from the genus Libyostrongylus, which has a knob on the larvae tip tail (EDERLI et al., 2008a, b) (Figures 5A, B). The total length of the infective larvae is not a good parameter to distinguish the species due to the similarity between nematodes. Libyostrongylus douglassii has a total length of 874.33 µm ± 33.80, ranging from 784.47 to 957.90 µm; L. dentatus784.47 µm ± 43.63, ranging from 735.84 to 947.01 µm and C. struthionis 598.25 µm ± 25.15, ranging from 511.92 to 642.41 µm (EDERLI et al., 2008a, c).

Figure 5. Posterior end of infective larvae. (A) Libyostrongylus douglassii; (B) Libyostrongylus dentatus; and (C) Codiostomum struthionis. 

The infective larvae of L. douglassii have a less-rounded cephalic end and became thinner more abruptly than L. dentatus infective larvae (BONADIMAN et al., 2006), while the infective larvae of C. struthionis have a flat, rounded cephalic end (EDERLI et al., 2008c). These parameters are not reliable in the specific diagnosis of these infective larvae due the similarity between the cephalic ends.

Libyostrongylus douglassii is easily distinguished from L. dentatus and C. struthionis by the infective larvae sheath tail: it is short with an acute termination, differing from the other 2 species, which have a long and filamentous sheath tail (Figure 5). The differential diagnosis between these 2 species is made by the observation of the shape of the larvae tail: L. dentatus has a knob in the tip of the tail (Figure 5B), while C. struthionis has an acute termination in the tip of the tail (Figure 5C) (EDERLI et al., 2008a, c). The comparison of the mean lengths of the infective larvae sheaths tail of L. douglassii and L. dentatus was considered extremely significant (EDERLI et al., 2008a); however, this parameter must be associated with the tail morphology of the larvae, because L. dentatus can be confused with C. struthionis, as mentioned previously. Due to these similarities, the identity of infective larvae can be unclear in fecal examinations, resulting in an error in the diagnosis. This misdiagnosis may be occurring with C. struthionis because there are few reports of this nematode in ostrich flocks (POPOVA, 1955; SOTIRAKI, et al., 2001; PONCE GORDO et al., 2002; EDERLI et al., 2008a). A recent reported the occurrence of C. struthionis, correlating its presence with dry and rainy seasons. However, the authors demonstrated images of infective larvae of L. dentatus, with a knob on the larvae tip tail and a long and filamentous sheath tail (FAGUNDES et al., 2012).

Below is a key to the identification of the infective larvae of nematodes of the genera Libyostrongylus and Codiostomum.

Conclusion

It is possible to distinguish between species of ostrich parasites by the morphological and morphometric characteristics of both adult nematodes and infective larvae, thus facilitating the diagnosis, ecology, systematic and epidemiology study of these parasites.

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Received: April 24, 2014; Accepted: July 7, 2014

*Corresponding author: Nicole Brand Ederli, Campus Universitário do Marajó-Breves, Universidade Federal do Pará – UFPA, Av. Anajás, s/n, Bandeirantes, CEP 68800-000, Breves, PA, Brasil e-mail: ederli@ufpa.br

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