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Revista Brasileira de Parasitologia Veterinária

Print version ISSN 0103-846XOn-line version ISSN 1984-2961

Rev. Bras. Parasitol. Vet. vol.25 no.3 Jaboticabal July/Sept. 2016  Epub Aug 25, 2016

http://dx.doi.org/10.1590/S1984-29612016046 

Research Note

Molecular and morphological evidence of Taenia omissa in pumas (Puma concolor) in the Peruvian Highlands

Evidência molecular e morfológica de Taenia omissa em onça-pardas (Puma concolor) dos Andes Peruanos

Luis Antonio Gomez-Puerta1  * 

Virgilio Alarcon2 

Joel Pacheco3 

Francisco Franco3 

Maria Teresa Lopez-Urbina1 

Armando Emiliano Gonzalez1 

1School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos – UNMSM, Lima, Peru

2Facultad de Agronomía y Zootecnia, Universidad Nacional San Antonio Abad del Cuzco – UNSAAC, Cuzco, Peru

3Instituto Veterinario de Investigaciones Tropicales y de Altura, Universidad Nacional Mayor de San Marcos – UNMSM, Sede Marangani, Cuzco, Perú


Abstract

A total of 41 cestodes were collected during necropsy examination on 2 pumas (Puma concolor) that were found in 2 communities in Canchis province, Cuzco region, Peru, at 4500 meters above sea level (Peruvian Andes). The cestodes were evaluated morphologically and molecularly. A fragment of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) was used as a genetic marker. All the cestodes were identified as Taenia omissa. In the present report, we give a brief description by molecular and morphological diagnosis of the cestodes and compare nucleotide sequences with previous isolates from GenBank. Upon comparison, the sequences showed a difference in the cox1 gene of 5.1 to 5.3% with other teniids sequences. This finding constitutes the first report of T. omissa in Peru and expands the geographic distribution of this parasite.

Keywords:  Taenia omissa; cestode; taeniid; puma; Puma concolor; cytochrome C oxidase subunit 1 gene

Resumo

Um total de quarenta e um cestóides foram coletados durante a necropsia de duas onça-pardas (Puma concolor) encontradas em duas comunidades na província de Canchis, em Cuzco, a 4500 metros acima do nível do mar, nos Andes peruanos. Os cestóides foram avaliados morfologicamente e molecularmente. Um fragmento do gene citocromo C oxidase subunidade 1 (cox1) foi utilizado como marcador genético. Todos os cestóides foram identificados como Taenia omissa. No presente relato, dá-se uma breve descrição dos cestóides e compara-se sequências de nucleotídeos com isolados anteriores presentes no GenBank. Após a comparação, as sequências mostraram uma diferença de 5,1-5,3% entre o gene cox1 e outras sequências de tênias. Esse achado constitui o primeiro relato de T. omissa no Peru e amplia a informação sobre a distribuição geográfica deste parasita.

Palavras-chave:  Taenia omissa; cestoide; tênia; onça-parda; Puma concolor; gene citocromo C oxidase subunidade 1

The genus Taenia Linnaeus, 1758 (Cestoda: Taeniidae) includes approximately 45 established species (HOBERG, 2006; LAVIKAINEN et al., 2008; ROSSIN et al., 2010; HAUKISALMI et al., 2011). Adult stages of these tapeworms develop in the small intestine of carnivorous mammals, and their metacestodes develop in different tissues of herbivorous or omnivorous mammals (ABULADZE, 1964). Many species of felids can act as definitive hosts for at least 14 species of Taenia (LOOS-FRANK, 2000).

The puma (Puma concolor Linnaeus, 1771), also called the mountain lion or cougar, is a large wild felid whose range extends from northern British Columbia in Canada to southern Chile and Argentina. In Peru, pumas are distributed from the rainforest to the Andes mountains, and can be found at altitudes as high as 5800 meters above sea level (LÓPEZ-GONZÁLEZ & GONZÁLEZ-ROMERO, 1998). Many studies about parasites in pumas have now been published (RAUSCH et al., 1983; WAID & PENCE, 1988; RICKARD & FOREYT, 1992; FOSTER et al., 2006; DARE & WATKINS, 2012). However, most of these studies were conducted in North America (RAUSCH et al., 1983; WAID & PENCE, 1988; RICKARD & FOREYT, 1992; FOSTER et al., 2006; DARE & WATKINS, 2012). Approximately 9 species of tapeworms have been found in pumas to date (SCHMIDT & MARTIN, 1978; D’ALESSANDRO et al., 1981; RAUSCH et al., 1983; FOSTER et al., 2006; VIEIRA et al., 2008), of which only four species have been found in South America: Echinococcus oligarthrus and Hydatigera taeniaeformis in Brazil (VIEIRA et al., 2008); and Taenia omissa and Spirometra sp. in Brazil and Paraguay (SCHMIDT & MARTIN, 1978; VIEIRA et al., 2008).

The present study confirms morphologically and molecularly the occurrences of T. omissa parasitizing two adult male pumas in Cuzco, Peru. This finding represents the first report of T. omissa in Peru.

In May and September of 2013, 2 adult male pumas were found dead, apparently killed by poachers, on two farms that were operated as alpaca production systems, located in the Pumaconca (14°18ʼ51.73ʼʼS, 71°09ʼ07.41ʼʼW) and Abra La Raya (14°28ʼ43.03ʼʼS, 71°01ʼ41.34ʼʼW) communities, in the province of Canchis, Cuzco region in Peru, at 4500 meters above sea level. The carcasses of the two pumas were donated for necropsy by the Technical Administration for Forestry and Wildlife of Peru to the veterinary research center IVITA-Marangani, at the Universidad Nacional Mayor de San Marcos. During the necropsy, twenty-eight and thirteen complete cestodes were collected from the small intestines, respectively. The parasites were fixed in 4% formaldehyde and then preserved in 70% ethanol. Some gravid proglottids were preserved in absolute ethanol for molecular studies.

Scoleces were mounted in Berlese’s medium to facilitate observation and measurement of rostellar hooks. The rostellar hooks were measured in accordance with the parameters described by Haukisalmi et al. (2011). Both mature and gravid proglottids were stained with Semichon’s acetocarmine stain and were dehydrated in an ascending alcohol series up to absolute ethanol. Subsequently, the samples were cleared in clove oil and terpineol, and then mounted in Canada balsam.

Photographs were taken using a Carl Zeiss microscope (Axioskop 40). Measurements were made using image analysis software (Leica IM50, version 4.0 R117). The measurements are reported in micrometers unless otherwise stated. Measured characteristics are given as range, with averages and standard error (SE) values in parentheses. The parasite taxonomic nomenclature used in this study follows Loos-Frank (2000) and Rausch (1994). The host taxonomic nomenclature follows Currier (1983).

Total DNA was extracted from three tapeworms from the puma from Pumaconca (To1, To2 and To3) using Chelex100, in accordance with the methodology described by Gadau (2009) with a minor modification. Tissue samples from gravid proglottids of approximately 1-3 mm3 were put into 0.2 mL plastic vials and were kept at 37 °C for 30 minutes. The tissue was then completely dried, and 100 µl of 5% Chelex solution and 1 µl of proteinase K (20 mg/mL) were added into the vials. The vials were then incubated in a thermocycler using the following program: 1 hour at 57 °C, 10 minutes at 95 °C, 1 minute at 37 °C, 10 minutes at 95 °C, and finally 15 minutes at 15 °C. The DNA samples were then stored at –70 °C until use.

PCR was used to amplify an approximately 400-bp fragment of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) using the primers JB3 and JB4.5 (BOWLES & MCMANUS, 1994). The PCR solution was prepared in a 50 µl volume containing 4 µl of template DNA, 0.25 µM of each primer and GoTaq® Green Master Mix, 2X (Promega, Madison, WI, USA). A three-step thermal process consisting of 94 °C for 30 seconds, 55 °C for 30 seconds and 72 °C for 30 seconds was repeated 36 times to amplify the short fragment of cox1 (LIU et al., 2011). The PCR products were analyzed by means of electrophoresis on 1.5% agarose gel with ethidium bromide staining. The PCR products were then sequenced using the Big DyeTM Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA, USA) and an ABI 3100 automated sequencer (Applied Biosystems). The sequences were assembled using the ChromasPro 1.7.6 software (TECHNELYSIUM, 2015). All the sequences were compared with a reference sequence in GenBank using ClustalX (CLUSTAL, 2015). A phylogenetic tree was constructed by means of the neighbor-joining method with the Kimura two-parameter distance, using the MEGA6 software (MEGA, 2015) (TAMURA et al., 2004; TAMURA et al., 2013). Unique nucleotide sequences of the partial cox1 gene of T. omissa from these pumas were deposited in the GenBank database under accession numbers KR095312, KR095313 and KR095314.

All the cestodes studied (41) were identified as T. omissa. The puma from Pumaconca was infected with a total of 28 cestodes (11 mature and 17 immature tapeworms). The puma from Abra La Raya was infected with a total of 13 tapeworms (9 mature and 4 immature tapeworms).

The immature tapeworms were 2.6-7.6 (4.4; SE: 0.3) cm in total length and 2.1-5.0 (2.9; SE: 0.2) mm in maximum width. All immature tapeworms did not show internal organs. The mature tapeworms had a strobila of 19.7-39.5 (29.7; SE: 2.5) cm in length, and 7.5-9.0 (8.2; SE: 0.2) mm in maximum width. The scoleces measured 977-1312 (1106; SE: 77.2) μm in diameter. Each scolex had four muscular suckers measuring 319-581 (467; SE: 43.2) μm in diameter. Each scolex had a rostellum armed with two rows of hooks (21 to 23 hooks in each row). The rostella measured 457-552 (508; SE: 20.1) μm in diameter. Large hooks measured 243-289 (266; SE: 4.4) μm in length and small hooks 186-229 (208; SE: 3.8) μm (Figure 1A-C). Additional morphological characteristics of the hooks are shown in Table 1.

Figure 1 (A) Rostellum of a specimen of Taenia omissa collected from Puma concolor in Peru. Scale bar = 200 μm; (B) Large hook. Scale bar = 100 μm; (C) Small hook. Scale bar = 100 μm; (D) Mature proglottid. Ov = ovarium; T = testes; Cs = cirrus sac; Vi = vitellarium and Mg = Mehlis’ gland. Scale bar = 2.0 mm. 

Table 1 Hook measurements of Taenia omissa (in µm) isolated from pumas in the Andes mountain range of Peru. Selected parameters were based on the system used by Haukisalmi et al. (2011)

Taenia omissa
N Mean Range
Large hooks 21-23 hooks
Total length (TL) 60 266 243-289
Total width (TW) 20 122 105-136
Basal length (BL) 20 190 173-207
Apical length (AL) 20 125 108-148
Guard length (GL) 20 52 38-63
Guard width (GW) 20 35 29-40
Blade curvature (BC) 20 33 30-36
Handle width (HW) 20 37 28-48
Small hooks 21-23 hooks
Total length (TL) 60 208 186-229
Total width (TW) 20 102 88-113
Basal length (BL) 20 149 135-162
Apical length (AL) 20 96 79-114
Guard length (GL) 20 39 28-49
Blade curvature (BC) 20 29 28-30

N = number.

The width of the mature proglottid was greater than its length (Figure 1D). The mature proglottid was 4.8-5.6 (5.3; SE: 60.1) mm long by 1.8-2.8 (2.3; SE 75.9) mm wide. Each mature proglottid had one set of genital organs. The genital pores alternated irregularly, and were positioned in the middle of the lateral margins of the proglottids. The genital atrium measured 210-239 (227; SE: 5.7) μm wide. The number of testes ranged from 315 to 378, with testes distributed anteriorly and laterally to the ovary between longitudinal osmoregulatory canals. The testes were subspherical and measured 43-74 (56; SE: 1.2) μm in diameter. The cirrus sacs were elongate and measured 357-472 (415; SE: 24.3) μm long by 122-155 (142; SE: 7.2) μm wide, extending across the longitudinal ventral canal. The vagina opened posteriorly to the cirrus sac. The ovaries were bilobate and measured 837-1137 (941; SE: 28.1) μm long by 285-640 (410; SE: 33.7) μm wide, and were distributed in the anterior half of the proglottid. The vitellarium was situated posteriorly to the ovary and measured 522-724 (600; SE: 20.0) μm long by 64-104 (83; SE: 4.2) μm wide. Mehlis’ gland was oval or subspherical and measured 89-123 (109; SE: 5.2) μm long by 80-116 (96; SE: 4.8) μm wide.

Nucleotide sequences of the cox1 gene were generated for PCR-positive isolates from three T. omissa (To1, To2 and To3). The first two were from the Pumaconca puma, and the third was from the Abra La Raya puma. The genetic identity of T. omissa was calculated from the alignments of the nucleotide sequences of the cox1 gene (Figure 2 and 3). Sequences of T. omissa from this study (GenBank Nos. KR095312 (To1), KR095313 (To2) and KR095314 (To3)) had single nucleotide differences of 0.7% between each other. However, the sequences for the To1 and To3 isolates were identical. All the sequences were compared with a sequence previously published for this species of tapeworm that had been collected from a puma in Canada (GenBank No. JX860631) (LAVIKAINEN et al., 2013). The isolate from Canada differed with regard to cox1 by 5.1 – 5.3% from the sequences in this study.

Figure 2 Genetic diversity in Taenia omissa sequences of the cox1 gene in pumas. Dots (.) denote nucleotides identical to those of the T. omissa control (JX860631). Sequences from pumas from Pumaconca (KR095312 and KR095313) and from Abra La Raya (KR095314) 

Figure 3 Phylogenetic relationship for Taenia species, based on cox1 gene. Neighbor-joining tree inferred by means of Kimura two-parameter distances from the partial cox1 gene. Sequences from GenBank were used as references. Sequences marked (*) were obtained in this study. The scale bars are proportional to the number of substitutions per site. 

Different species of Taenia have been reported in pumas, including Taenia multiceps, Taenia hydatigena, Taenia ovis, Taenia krabbei, T. omissa and H. taeniaeformis (Syn. Taenia taeniaeformis see Nakao et al., 2013 and Haukisalmi, 2016) (LUHE, 1910; RAUSCH et al., 1983; VIEIRA et al., 2008; WAID & PENCE, 1988). The main criteria for the morphological diagnosis of taeniid cestodes are measurements and numbers of rostellar hooks (Table 2) (RISER, 1956; ABULADZE, 1964; LOOS-FRANK, 2000). Based on the morphological and molecular characteristics of the specimens in this study, which coincided with data reported by other authors (LUHE, 1910; RISER, 1956; JONG, 1966; RAUSCH, 1981; RAUSCH et al., 1983; LOOS-FRANK, 2000; DARE & WATKINS, 2012; LAVIKAINEN et al., 2013), we conclude that the species isolated in this study corresponded to T. omissa (Table 3). This tapeworm has been reported in pumas in Canada, the USA, Paraguay and Brazil (RISER, 1956; SCHMIDT & MARTIN, 1978; VIEIRA et al., 2008; DARE & WATKINS, 2012). The current study demonstrates additional geographic distribution for this parasite.

Table 2 Characetristics of rostellar hooks of Taenia species reported in Puma concolor

T. omissaa T. ovisb T. krabbeib T. hydatigenab T. multicepsb H. taeniaeformisb
Number of hooks 42-46 30-34 26-36 28-36 22-30 34-36
Larger hookc 243-289 170-191 137-195 191-218 157-177 370-402
Small hookc 186-229 111-127 85-141 118-143 98-136 210-261

aPresent study.

b Loos-Frank (2000).

cLength in micrometers.

Table 3 Reported characteristics of rostellar hooks of Taenia omissa

Present study Dare & Watkins (2012) Loos-Frank (2000) Rausch et al. (1983) Rausch (1981) Jong (1966) Riser (1956) Luhe (1910)
Number of hooks 42-46 38-44 40
Larger hooka 266 (243-289) 246.5-276.1 270-297 223-273 232-254 253 240-280 270-290
Small hooka 208 (186-229) 193.7-221.8 192-223 165-223 183-210 203 180-210 190-220

aLength in micrometers.

Morphologically, T. omissa is characterized by proglottids that are wider than they are long, as was demonstrated in this study (Figure 1D). This is also a morphological characteristic of H. taeniaeformis, a common cestode in felids, including pumas (ABULADZE, 1964). However, T. omissa differs from H. taeniaeformis in some morphological characteristics such as the numbers (42-46 vs. 34-36, respectively) and larger rostellar hooks in H. taeniaeformis (Table 2) (RISER, 1956; ABULADZE, 1964; LOOS-FRANK, 2000).

Nucleotide sequences of the cox1 gene showed intraspecific variation of 0.7-5.3% between all the T. omissa isolates, including the isolate from Canada (JX860631). Similar variations have been demonstrated in other taeniid cestodes. Lavikainen et al. (2008) performed molecular phylogeny on various taeniid cestodes and found an intraspecific variation of 0.0-3.4% in Taenia mustelae, 0.0-6.8% in Taenia polyacantha and 1.3-9.8% in H. taeniaeformis (LAVIKAINEN et al., 2008).

The life cycle of T. omissa has been described by Forrester & Rausch (1990), they identified metacestodes collected from white-tailed deer (Odocoileus virginianus) in southern Florida, and morphological analysis showed the metacestodes to be T. omissa cysticerci (FORRESTER & RAUSCH, 1990). Currently, two species of deer are known to be involved in the life cycle of T. omissa, i.e. Odocoileus hemionus and O. virginianus (JENSEN et al., 1982; FORRESTER & RAUSCH, 1990; PYBUS, 1990). In Peru, O. virginianus has wide geographic distribution that includes the Andes mountain range and overlaps with the geographic distribution of the puma (SMITH, 1991). Therefore, we predict that O. virginianus is involved in the life cycle of T. omissa in Peru. Futures research will be needed to identify the animal species involved as an intermediate host in the life cycle of T. omissa in the Peruvian highlands.

Acknowledgements

The authors wish to thank Guissela Zans and Maria de la Barra from the Technical Administration for Forestry and Wildlife of Peru (Administracion Tecnica Forestal y de Fauna Silvestre Cusco, Ministerio de Agricultura y Riego) for donating the pumas used in this study.

§Cysticercosis Working Group in Peru

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Received: January 04, 2016; Accepted: June 13, 2016

*Corresponding author: Luis Antonio Gomez-Puerta. Department of Veterinary Epidemiology and Economics, School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos – UNMSM, Avenida Circunvalacion, 2800, San Borja, Lima 41, Perú. e-mail: lucho92@yahoo.com

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