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Revista Brasileira de Parasitologia Veterinária

Print version ISSN 0103-846XOn-line version ISSN 1984-2961

Rev. Bras. Parasitol. Vet. vol.28 no.1 Jaboticabal Jan./Mar. 2019  Epub Feb 21, 2019 

Original Article

Helminths Assemblage of the bare-faced ibis, Phimosus infuscatus (Lichtenstein, 1823) (Pelecaniformes: Threskiornithidae), in southern Brazil

Helmintos de Phimosus infuscatus (Lichtenstein, 1823) (Pelecaniformes: Threskiornithidae) no extremo sul do Brasil

Simone Scheer1

Carolina Silveira Mascarenhas1 

Márcia Raquel Pegoraro de Macedo1 

Gertrud Muller1 

1 Laboratório de Parasitologia de Animais Silvestres – LAPASIL, Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas – UFPel, Pelotas, RS, Brasil


Birds act as hosts for a variety of parasites, many of these are unreported. The literature provides scant information on the helminth fauna of Phimosus infuscatus. The presence of helminths were investigate in 28 birds from Pelotas, Capão do Leão, and Rio Grande in Rio Grande do Sul. The preparation and identification of helminths followed protocols. Prevalence (P%), mean intensity of infection (MII), and mean abundance (MA) were estimated. The following helminths were found: Hystrichis acanthocephalicus , Dioctophyme renale (larva), Porrocaecum heteropterum , Baruscapillaria sp., Aproctella carinii , Paradeletrocephalus minor, and Cyathostoma sp. (Nematoda); Echinostomatidae gen. sp., Tanaisia valida, and Athesmia sp. (Trematoda: Digenea) and Megalacanthus sp. (Cestoda). The most prevalent species were H. acanthocephalicus, P. heteropterum, Megalacanthus sp., and Echinostomatidae gen. sp. and Megalacanthus sp. had the highest MII and MA. There was a significant difference in the prevalence of H. acantocephalicus between female and male bird hosts. We report Echinostomatidae gen. sp., T. valida, Athesmia sp., Cyathostoma sp., A. carinii, P. minor, D. renale (larva), Baruscapillaria sp., and Megalacanthus sp. for the first time in P. infuscatus in Brazil.

Keywords:  Nematoda; Trematoda; Digenea; Cestoda; parasitological index


As aves atuam como hospedeiros para uma ampla variedade de parasitos, muitos destes ainda desconhecidos. Foram examinadas 28 aves, provenientes dos municípios de Pelotas, Capão do Leão e Rio Grande. A coleta, preparação e identificação dos helmintos seguiu bibliografia específica. A assembleia de helmintos foi analisada através dos índices de prevalência (P%), intensidade média de infecção (IMI) e abundância (AM). A assembleia de helmintos de P. infuscatus estava composta por Nematoda: Hystrichis acanthocephalicus , Dioctophyme renale (larva), Porrocaecum heteropterum , Baruscapillaria sp., Aproctella carinii , Paradeletrocephalus minor, Cyathostoma sp.; Digenea: Echinostomatidae gen. sp., Tanaisia valida, Athesmia sp. e Megalacanthus sp. (Cestoda). As espécies mais prevalentes foram: H. acanthocephalicus, P. heteropterum , Megalacanthus sp. e Echinostomatidae gen. sp. A maior IMI e AM foi de Megalacanthus sp., onde observou-se diferença significativa na prevalência de H. acantocephalicus em hospedeiros fêmeas. Os helmintos Echinostomatidae gen. sp., T. valida, Athesmia sp., Cyathostoma sp., A. carinii, P. minor, D. renale (larva), Baruscapillaria sp., e Megalacanthus sp. são registrados pela primeira vez em P. infuscatus no Brasil.

Palavras-chave:  Nematoda; Trematoda; Digenea; Cestoda; índices parasitológicos


Wildlife birds act as hosts for a wide variety of parasites. To date, many of which are unreported birds, especially the aquatic ones, constitute one of the fundamental groups for ecosystems, and act as excellent environmental sentinel species. They provide nourishment for other species, and help in the control of invasive plants, seed dispersal, and contribute to pollination ( SICK, 2001 ). Moreover, aquatic birds can also disseminate pathogens including viruses, bacteria, and parasites. It is estimated that birds are the ones with the highest parasite diversity among all vertebrates ( DOBSON et al., 2008 ), often these organisms are not seen as important components in terms of biodiversity ( AMATO & AMATO, 2010 ).

The bare-faced ibis Phimosus infuscatus (Lichtenstein, 1823) (Pelecaniformes: Threskiornithidae) is widely distributed in South America including Guyana, Venezuela, Bolivia, Paraguay, Argentina, Uruguay, and Brazil ( SICK, 2001 ). It inhabits shallow water areas such as flooded fields, wetlands, lakes, rice paddies, and urban environments ( BELTON, 2003 ).

There are reports of helminths parasitizing birds from the Threskiornithidae in different parts of the world. However, the helminth fauna of Phimosus infuscatus is little known ( Table 1 ).

Table 1 Helminth fauna of the bare-faced ibis Phimosus infuscatus in South America.  

Helminth Location Reference
Nematoda Hystrichis acantocephalicus Molin, 1861 Brazil Vicente et al. (1995) ; Scheer et al. (2017)
Eustrongylides ignotus Jagerskiold, 1909 Brazil
Tetrameres sp. Brazil Spalding and Forrester, (1993)
Porrocaecum sp. Brazil Vicente et al. (1995)
Porrocaecum heteropterum Diesing, 1851 Brazil Vicente et al. (1995)
Trematoda Stomylotrema sp. Brazil Travassos and Freitas (1942)
Cestoda Chimaerula bonaiGeorgiev e Vaucher, 2000 Paraguay Georgiev and Vaucher, (2000)

The objective of this study was to investigate the prevalence, mean abundance and mean intensity of infection of helminth parasites in P. infuscatus, and compare infections between male and female hosts.

Materials and Methods

Twenty-eight birds (15 males and 13 females) were examined postmortem, of which 21 were captured between December 2015 and February 2016 in the municipality of Rio Grande, State of Rio Grande do Sul, southern Brazil (31°51’12.1”S, 52°18’48.0”W). The other birds (n = 7) were donated by the Núcleo de Reabilitação da Fauna Silvestre and Centro de Triagem de Animais Silvestres da Universidade Federal de Pelotas (NURFS-CETAS/UFPel), where they were they had died. These birds were originated from the municipalities of Pelotas (n = 5) (31°46’19”S; 52°20’33”W) and Capão do Leão (n = 2) (31°46”3”S; 52°26’55”W).

Euthanasia and sampling of these birds were authorized by the Instituto Chico Mendes de Conservação da Biodiversidade – (ICMBIO nº 507541). The research proposal was approved by the Comissão de Ética em Experimentação Animal (CEEA/UFPel nº 8876).

At necropsy, the oral cavity, nostrils, trachea, lungs, esophagus, gizzard, proventriculus, small intestine, cecum, large intestine, cloaca, pancreas, spleen, heart, kidneys, air sacs, and reproductive system were examined and helminths were collected. For parasite sampling, organs and their contents were washed using sieves with 150 μm mesh pores.

Helminths were fixed in AFA (ethyl alcohol, formaldehyde, and acetic acid), and preserved in ethanol 70° GL. Nematodes were clarified with Amann's lactophenol. Trematodes and cestodes were stained with Delafield hematoxylin or with Langeron’s carmine ( AMATO & AMATO, 2010 ).

Nematodes were identified according to the taxonomic keys published by Lengy (1969) , Hendricks et al. (1969) , Measures & Anderson (1985) , Vicente et al. (1995) , Hartwich (2009) , Anderson et al. (2009), and Gibbons (2010) . Morphological identification of digenetic trematodes was based on the taxonomic keys provided by Kanev et al. (2002) , Kostadinova (2005) , Pojmanska (2008) , and Lunaschi et al. (2015) . The identification of cestode was according to Bona (1994) .The estimated parasitological indices were: Prevalence (P%), Mean Intensity of infection (MII), Mean Abundance (MA) and Range (R) ( BUSH et al., 1997 ).

P% and MII were compared between males (n = 15) and females (n = 13). P% was compared using the Chi-square test (X2) whereas MII was compared using the Bootstrap interval (BCα, p<0.05) in the software “Quantitative Parasitology - QP 3.0” ( RÓZSA et al., 2000 ).

Voucher specimens were deposited in ʽʽLaboratório de Parasitologia de Animais Silvestres da Universidade Federal de Pelotasˮ (LAPASIL/UFPel), Pelotas, RS, Brazil, under accession numbers 636-706 and 712.


All birds were parasitized by at least one species of helminth. Nematodes, cestodes, and digenetic trematodes had prevalences of 92.86%, 82.14% and 75%, respectively. Helminths assemblage of P. infuscatus in southern Brazil included the following: Nematoda: Hystrichis acanthocephalicus (Molin, 1861), Dioctophyme renale (Goeze, 1782) (larva), (Enoplida: Dioctophymatidae), Baruscapillaria sp. (Enoplida: Trichuridae), Cyathostoma sp. (Strongylida: Syngamidae), Paradeletrocephalus minor (Molin, 1861) (Strongylida: Deletrocephalidae), Porrocaecum heteropterum (Diesing, 1851) (Ascaridida: Ascarididae), Aproctella carinii (Pereira & Vaz, 1933) (Spirurida: Onchocercidae); Trematoda, Digenea: Echinostomatidae gen. sp., Tanaisia valida (Freitas, 1951) (Eucotylidae), Athesmia sp. (Dicrocoeliidae); and Cestoda: Megalacanthus sp. (Cyclophyllidea: Dilepididae).

The helminths H. acanthocephalicus, Megalacanthus sp., Echinostomatidae gen. sp., and P. heteropterum were the most prevalent, and occurred in 89.28%, 89.28%, 82.14% and 50% of birds necropsied, respectively ( Table 2 ).

Table 2 Helminths of Phimosus infuscatus (n=28) in southern Brazil and their respective infection sites and parasitological indexes.  

Helminths Site of Infection P (%) MII (SD)* MA (SD)* R
Hystrichis acanthocephalicus Proventricle 89.28 3.87 (±5.26) 3.17 (± 5.0) 1-18
Dioctophyme renale (larva) Esophagus 3.57 1 0.03 1
Porrocaecum heteropterum Small intestine, gizzard 50 29.5 (±74.65) 12.64 (±50.0) 2-265
Baruscapillaria sp. Small intestine 1.71 13 (±11.53) 1.39 (±5.16) 4-26
Aproctella carinii Small intestine 7.14 1.5 (±0.71) 0.11 (±0.42) 1-2
Paradeletrocephalus minor Abdominal cavity 3.57 1 0.03 1
Cyathostoma sp. Trachea 3.57 10 0.35 10
Echinostamatidae gen. sp. Small intestine 82.14 24.23 (±28.27) 18.17 (±26.58) 2-135
Tanaisia valida Renal ducts 3.57 5 0.17 5
Athesmia sp. Renal ducts 3.57 11 0.39 11
Megalacanthus sp. Small intestine 89.28 48.26 (±64.28) 39.64 (±61.0) 1-278

P% (Prevalence), MII (Mean intensity of infection), MA (Mean abundance), R (Range);

*SD = Standard Deviation.

Although H. acanthocephalicus occurred in 89.28% of the birds, it had low levels of MII (3.87) and MA (3.17). In contrast, Megalacanthus sp. presented the highest parasitological indexes of the assemblage: MII= 48.26 helminths/host (1-278), MA= 39.64 and Echinostomatidae gen. sp. and P. hetoropterum had similar MII and MA ( Table 2 ).

With regard to the prevalence of helminth infections in male birds and female birds, it was observed that H. acanthocephalicus, P. heteropterum, Baruscapillaria sp., Echinostomatidae gen. sp., and Megalacanthus sp. were common in both males and females. There were no significant difference in the P% and MII of these helminths according to the host’s genders, except for H. acanthocephalicus, which was significantly more prevalent in females (P=100%) than in males (P=66.7%) ( Table 3 ). The helminths Cyathostoma sp., T. valida, and Athesmia sp. were found only in females, whereas D. renale (larva), P. minor, and A. carinii were found only in males with low infection rates ( Table 3 ).

Table 3 Parasitological indexes in male and female Phimosus infuscatus in southern Brazil.  

Helminths Parasitological Indexes Hosts’ Sex
Male (n = 15) Female (n = 13)
Hystrichis acanthocephalicus P% 66.7 100 *
MII (SD) 3.4 (±2.5) 4.23 (±6.76)
MA (SD) 2.26 (±2.60) 4.23 (±6.76)
R 1-10 1-26
Dioctophyme renale (larva) P% 6.66 0
MII (SD) 1 0
MA (SD) 0.06 (±0.26) 0
R 1 0
Porrocaecum heteropterum P% 53.3 30,8
MII (SD) 9.88 (±9.95) 68.75 (±130.84)
MA (SD) 5.26 (±8.69) 21.15 (±73.28)
R 3-34 2-265
Baruscapillaria sp. P% 6.7 15.4
MII (SD) 9 15 (±15.56)
MA (SD) 0.6 (±2.32) 2.3 9 (±7.20)
R 9 4-26
Aproctella carinii P% 13.33 0
MII (SD) 1.5 (±0.71) 0
MA (SD) 0.2 (±0.56) 0
R 1-2 0
Paradeletrocephalus minor P% 6.66 0
MII (SD) 1 0
MA (SD) 0.06 (±0.26) 0
R 1 0
Cyathostoma sp. P% 0 7.7%
MII (SD) 0 10
MA (SD) 0 0.76
R 0 10
Echinostomatidae gen. sp. P% 75 76.9
MII (SD) 21.90 (±25.68) 26,8 (±32,09)
MA (SD) 18.13 (±23.91) 31 (±30.17)
R 3-92 5-135
Tanaisia valida P% 0 6.66
MII (SD) 0 5
MA (SD) 0 0.33 (±1.39)
R 0 5
Athesmia sp. P% 0 6.66
MII (SD) 0 11
MA (SD) 0 0.73 (±3.05)
R 0 11
Megalacanthus sp. P% 73.3 92.3
MII (SD) 24.09 (±40.07) 70.42 (±75.39)
MA (SD) 18.86 (±35.61) 65.15 (±74.76)
R 1-137 2-278

P% (Prevalence), MII (Mean intensity of infection), MA (Mean abundance), R (Range), SD = Standard Deviation;

* significant value for the X2 test (p <0.05).


The P%, MII, and MA indexes of H. acanthocephalicus, P . heteropterum, Echimostomatidae gen. sp., and Megalacanthus sp. suggest that these helminths are common in P. infuscatus.

These indexes may reflect prey-predator interactions as these helminths have an indirect life cycle in which intermediate hosts and/or paratenic hosts are involved and may be part of the bird's diet.

Hystrichis spp. have been reported in many avian hosts and in several countries in lower prevalences ( KINSELLA et al., 1973 ; CANARIS et al., 2010). In Brazil, H. acanthocephalicus only was reported in the proventricular glands of P. infuscatus, P=89.28% ( SCHEER et al., 2017 ).

Porrocaecum spp. are ascarids that occur in the digestive tract of birds, reptiles, fish, and mammals ( HARTWICH, 2009 ). According to Digiani & Sutton (2001) , there are approximately 40 species of Porrocaecum reported to date, with the highest prevalence in wading birds from the Threskiornithidae that inhabit the Neotropical region.

In Guaminí and Rahue, Argentina, Digiani & Sutton (2001) reported P. heteropterum in the Plegadis chihi (Vieillot, 1817) (Pelecaniformes: Threskiornithidae) and Theristicus melanopis melanopis (Gmelin, 1789) in the small intestine of birds. In P. chihi, one of the seven birds was parasitized, in which 11 helminths were found. In T. melanopis (n=5), 2 birds were parasitized with P. heteropterum. Digiani & Sutton (2001) also examined 56 P. chihi from Punta Blanca, Argentina, and this helminth was not found in any of the birds necropsied. However, in the present survey, significant numbers of P. heteropterum were found in P. infuscatus ( Table 2 ). In Brazil, Vicente et al. (1995) reported P. heteropterum in the small intestine of P. infuscatus and T. caudatus (Boddaert, 1783) (Pelecaniformes: Threskiornithidae). However, information on parasitological indexes is not available.

In Brazil, cestodes belonging to the Dilepididae have been documented in several birds of the Threskiornithidae. Parasitological indexes have not been reported though ( BONA, 1975 ). Bona (1994) cited, for Brazil, Megalacanthus macracantha Furhrmann, 1908, in Belonopterus chilensis cayennensis (Molina, 1982) (Charadriiformes: Charadriidae) and Megalacanthus rostellata Furhmann, 1908 without identifying their host. In Paraguay, the cestode Chimaerula bonai was recorded in P. infuscatus with a prevalence of 100% (n=4) ( GEORGIEV & VAUCHER, 2000 ). In P. infuscatus, Megalacanthus sp. was present in all indexes analyzed, which suggests that this avian host plays an important role in the life cycle of the helminth in this geographic region ( Table 2 ).

Echinostomatidae Looss, 1899, consists of intestinal digeneans that occur in birds, mammals, and humans, and has a broad geographic distribution. It comprises 355 species and 50 genera. Due to its diversity, many authors report difficulties in the taxonomic identification of members of this family ( KOSTADINOVA, 2005 ). All specimens found parasitizing P. infuscatus belong to the same species, however it was not possible their identification into genus level with the references available. In Argentina, Digiani (2000) reported Dietziella egregia (Dietz, 1909) in P. chihi (n = 62) with prevalence of 46.7%, and MII = 82.35. Dronen & Blend (2008) described Patagifer lamothei in Eudocimus albus Linnaeus, 1758 (Pelecaniformes: Threskiornithidae) (n = 8) in the State of Texas, USA with P = 25% and MII = 6. Both birds have very similar habits to those of the avian host studied in our survey.

The other helminths found in P. infuscatus had low parasitological indexes. Cyathostoma sp., P. minor, D. renale (larva), T. valida, and Athesmia sp. were found infecting one host. This finding suggests that infections may have been accidental through ingestion of intermediate or paratenic hosts. The diet of P. infuscatus is composed of annelids, molluscs, and insects ( BELTON, 2003 ), and these invertebrates may participate in the life cycle of these helminths ( Table 4 ).

Table 4 Helminth parasites of Phimosus infuscatus in southern Brazil and their respective intermediate and paratenic hosts, considering the information available for life cycles of congeners species or species of the same family.  

Taxon Intermediate host Paratenic host References
Hystrichis acanthocephalicus Aquatic oligochaetes anurans Anderson (2000)
Porrocaecum heteropterum Aquatic oligochaetes Anderson (2000)
Dioctophyme renale (larva) Aquatic oligochaetes Anderson (2000)
Aproctela carinii Mosquitoes (Culicidae) Anderson (2000)
Baruscapillaria sp. Monoxene Anderson (2000)
Paradeletrocephalus minor Monoxene Anderson (2000)
Cyathostoma sp. Monoxene earthworms, mollusks, ants and beetles Atkinson et al. (2009)
Echinostomatidae gen. sp. Sweet and sweet gastropod molluscs larvae of anurans Esteban and Muñoz-Antoli (2009)
Athesmia sp. (Dicrocoeliidae) Mollusks and arthropods amphibians and reptiles Pojmanska (2008)
Tanaisia valida (Tanaisiinae) Mollusks Lunaschi et al. (2015)
Megalacanthus sp. (Dilepididae) Annelids, mollusks and arthropods Olsen (1974)

Nematodes Syngamidae Leiper, 1912, may be present in the respiratory and digestive tracts and body cavities of mammals, rodents, and birds. They are known to live in copula, except for Cyathostoma ( BORGSTEEDE & OKULEWICZ, 2001 ). According to Kanarek (2009) , there are six species of Cyathostoma that occur in birds of different orders such as Anseriformes, Charadriiformes, Casuariiformes, Ciconiiformes, Columbiformes, Coraciiformes, Galliformes, Gaviiformes, Gruiformes, Falconiformes, Passeriformes, Pelecaniformes, Psittaciformes, Strigiformes, Struthioniformes, and Sphenisciformes. Cyathostoma phenisci (Baudet, 1937) was reported in Pelecanus erythrorhynchus Gmelin, 1789 (Pelecanidae), in North America ( OVERSTREET & CURRAN, 2005 ). Nevertheless, these studies did not report infection rates. In the State of Florida, USA, Courtney & Forrester (1974) reported the occurrence of C. phenisci in Pelecanus occidentalis Linnaeus, 1766 (Pelecanidae) (n = 57), with an intensity of one to four nematodes which were present in the trachea, lungs, and air sacs. The results of the present study are similar to those reported by Courtney & Forrester (1974) since the prevalence of the infection by this nematode was considered low. The life cycle of Cyathostoma include paratenic hosts that can be ingested by P. infuscatus ( Table 4 ).

Deletrocephalidae Chitwood, 1969, comprises Deletrocephalus Diesing, 1851, and Paradeletrocephalus Freitas & Lent, 1947, which include species commonly found in the large intestine of ratites. They have been often reported in the greater rhea Rhea americana (Linnaeus, 1758) (Rheiformes: Rheidae) in Brazil, Argentina, and Europe ( GORDO et al., 2002 ; ACOMOLLI et al., 2006 ; HOFFMANN et al., 2009 ). The association between P. minor and P. infuscatus may be related to the bird’s feeding habits and habitat. The low parasitological indexes in P. infuscatus and the biology of members of Deletrocephalidae ( Table 4 ) suggest that infections may have been accidental.

Likewise, infection by a larva of Dioctophyme renale (Goeze, 1782) in an avian host may be considered accidental. This nematode is a parasite of wild and domestic mammals and has aquatic oligochaetes, anurans, and fish as intermediate and paratenic hosts, respectively ( Table 4 ) ( ANDERSON, 2000 ).

In aquatic birds in the same region, southern Brazil, Bernardon et al. (2017) documented Baruscapillaria sp. in Ardeidae, Egretta thula (Molina, 1782) (n = 6) and Ardea cocoi Linnaeus, 1766 (n = 5) (Pelecaniformes) with P = 66.6%, 40%, MA = 2.3 and 1.2 and MII = 3.5 e 3.0, respectively, differing in our research ( Table 2 ). The life cycle of Baruscapillaria obsignata (Madsen, 1945) is well-known ( ANDERSON, 2000 ) ( Table 4 ). Aproctella carinii and T. valida were reported in Passeriformes from same region with indexes of infection differing in our research ( MASCARENHAS et al., 2009 ; BERNARDON et al., 2016 )

In Argentina, in hosts Threskiornithidae, A. heterolechithodes was reported in T. caudatus (n = 1) with MII=15 ( LUNASCHI et al., 2015 ), and in P. chihi (n= 60) with P= 8.8% and MII=8.2 ( DIGIANI, 2000 ). In our research the indexes were similar ( Table 2 ).

The diet of P. infuscatus is composed of annelids, molluscs, and insects ( BELTON, 2003 ) which are the intermediate or paratenic hosts (or both) ( Table 4 ) of many of the helminths reported in this survey.

The presence of helminth infections in males and females of endothermic hosts may indicate that the highest prevalence is due to morphological, physiological, and behavioral aspects such as territorialism and social interaction which may vary throughout the life of the host ( POULIN, 1996 ).

The low prevalence of some helminth species may be related to low levels of natural infection and low availability of intermediate hosts in the environment ( ROBINSON et al., 2008 ).

In the State of Rio Grande do Sul, studies carried out in Turdus rufiventris Vieillot, 1818, (Passeriformes: Turdidae) (n=151) ( CALEGARO-MARQUES & AMATO, 2010 ) and in Phalacrocorax brasilianus (Gmelin, 1789) (Suliformes: Phalacrocoracidae) (n = 47) ( MONTEIRO et al., 2011 ) assessed the correlation between the gender of hosts and the occurrence of helminth infections. There were no differences between males and females in terms of the composition and parasitological indexes of helminths. These results are similar to those found in P. infuscatus and is attributed to the similar diet and the habitat shared by males and females.


The infection rates allow us to conclude that helminths Hystrichis acanthocephalicus, Porrocaecum heteropterum, Echinostomatidae gen. sp., and Megalacanthus sp. are common in the assembly of P. infuscatus from southern Brazil.

The nematodes Cyathostoma sp., Aproctella carinii, Paradeletrocephalus minor, Dioctophyme renale (larva), and Baruscapillaria sp., the digenetic trematodes Echinostomatidae gen. sp., Tanaisia valida, and Athesmia sp., and the cestode Megalacanthus sp. are reported for the first time in P. infuscatus in Brazil.

With regard to male and female avian hosts, both have a similar helminth fauna, except for H. acanthocephalicus, which was more prevalent in females.


We thank the Núcleo de Reabilitação da Fauna Silvestre/ Universidade Federal de Pelotas (UFPel) for the donation of birds, and the Instituto Chico Mendes de Conservação da Biodiversidade for the authorization to capture the birds for this research.


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Received: July 01, 2018; Accepted: January 03, 2019

Corresponding author: Simone Scheer. Laboratório de Parasitologia de Animais Silvestres – LAPASIL, Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas – UFPel, Campus Universitário Capão do Leão, CP 354, CEP 96010-900, Pelotas, RS, Brasil. e-mail:

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