Abstracts
In this study, we aimed to determine lignified tissue in young stems of Struthanthus vulgaris Mart. by infrared microspectroscopy and histochemical methods as well as by fluorescence microscopy. Struthanthus vulgaris Mart. is a mistletoe species that belongs to the Loranthaceae family. A brief anatomical description was also carried out. The first procedure for analysis was to elaborate anatomical cross sections (20-30 µm) from young stems before and after treatment with NaOH 1%. This procedure was applied to release possible low molecular mass phenolic compounds. Safranin-astra blue was used to distinguish anatomical tissues while Wiesner test enabled verification of lignified pericyclic fibers. Infrared microspectroscopy analysis confirmed the presence of lignin in this region according to the following spectral signals: 1600 (shoulder), 1511, 1453, 1338 and 1244 cm-1. Analyses of the cross section of young stems under fluorescence microscopy before and after treatment with NaOH 1% allowed us to confirm the presence of low mass phenolic compounds in the region of pericyclic fibers.
mistletoe species; fibers; lignifications
O objetivo deste estudo foi determinar tecidos lignificados em caules jovens de Struthanthus vulgaris Mart., por microspectroscopia no infravermelho, teste histoquímico e microscopia com fluorescência. Esta é uma espécie hemiparasita da família Loranthaceae. Foi também realizada uma breve descrição anatômica. O primeiro procedimento para a análise foi realizar os cortes anatômicos transversais (20-30 µm) do caule jovem, sendo feita a análise antes e após tratamento com NaOH 1% para liberar substâncias fenólicas de baixa massa molecular. Utilizou-se safranina azul de astra para identificar os tecidos anatômicos, enquanto o teste Wiesner permitiu verificar fibras pericíclicas lignificadas. A análise por microespectroscopia no infravermelho confirmou a presença de lignina nesta região por meio dos seguintes sinais espectrais em 1600 (ombro), 1511, 1453, 1338 e 1244 cm-1. As análises da seção transversal do caule jovem sob microscopia de fluorescência antes e após tratamento com NaOH 1% permitiram confirmar a presença de substâncias fenólicas de baixa massa molecular na região das fibras pericíclicas.
espécie hemiparasita; fibras; lignificação
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Publication Dates
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Publication in this collection
16 July 2013 -
Date of issue
June 2013
History
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Received
25 Sept 2012 -
Accepted
07 May 2013
